@article{bierlein_hedgespeth_azcarate-peril_stauffer_gookin_2021, title={Dysbiosis of fecal microbiota in cats with naturally occurring and experimentally induced Tritrichomonas foetus infection}, volume={16}, ISSN={["1932-6203"]}, DOI={10.1371/journal.pone.0246957}, abstractNote={The protozoal pathogen Tritrichomonas foetus infects the colon of domestic cats and is a major cause of chronic colitis and diarrhea. Treatment failure is common, but antibiotics may improve clinical signs in a subset of cats, leading researchers to question involvement of the colonic microbiota in disease pathogenesis. Studies performed in women with venereal Trichomonas vaginalis infections have revealed that dysbiosis of host microbiota contributes to pathogenicity with similar findings also found in mice with intestinal Tritrichomonas musculis The aim of this study was to characterize differences in the fecal microbiota of cats with and without naturally occurring T. foetus infection and in a group of kittens prior to and after experimentally induced infection. Archived fecal DNA from cats undergoing testing for T. foetus infection (n = 89) and experimentally infected kittens (n = 4; at pre-, 2 weeks, and 9 weeks post-infection) were analyzed by sequencing of 16S rRNA genes. Amongst the naturally infected population, the genera Megamonas and Helicobacter were significantly increased in prevalence and abundance in cats testing positive for T. foetus infection. In the group of four experimentally infected kittens, fecal samples post-infection had significantly lower abundance of genus Dialister and Megamonas and greater abundance of the class Betaproteobacteria and family Succinivibrionaceae. We hypothesize that T. foetus promotes dysbiosis by competition for fermentable substrates used by these bacteria and that metabolic byproducts may contribute to the pathogenesis of colonic inflammation and diarrhea. Future studies are warranted for the measurement of fecal concentrations of microbial and protozoal metabolites in cats with T. foetus infection for the identification of potential therapeutic targets.}, number={2}, journal={PLOS ONE}, author={Bierlein, Metzere and Hedgespeth, Barry A. and Azcarate-Peril, M. Andrea and Stauffer, Stephen H. and Gookin, Jody L.}, year={2021}, month={Feb} }
 @article{kuzma_gould_brown_collins_delborne_frow_esvelt_guston_leitschuh_oye_et al._2018, title={A roadmap for gene drives: using institutional analysis and development to frame research needs and governance in a systems context}, volume={5}, ISSN={2329-9460 2329-9037}, url={http://dx.doi.org/10.1080/23299460.2017.1410344}, DOI={10.1080/23299460.2017.1410344}, abstractNote={ABSTRACTThe deployment of gene drives is emerging as an alternative for protecting endangered species, controlling agricultural pests, and reducing vector-borne diseases. This paper reports on a workshop held in February 2016 to explore the complex intersection of political, economic, ethical, and ecological risk issues associated with gene drives. Workshop participants were encouraged to use systems thinking and mapping to describe the connections among social, policy, economic, and ecological variables as they intersect within governance systems. In this paper, we analyze the workshop transcripts and maps using the Institutional Analysis and Development (IAD) framework to categorize variables associated with gene drive governance and account for the complexities of socio-ecological systems. We discuss how the IAD framework can be used in the future to test hypotheses about how features of governance systems might lead to certain outcomes and inform the design of research programs, public engagement, and...}, number={S1}, journal={Journal of Responsible Innovation}, publisher={Taylor & Francis}, author={Kuzma, J. and Gould, F. and Brown, Z. and Collins, J. and Delborne, J. and Frow, E. and Esvelt, K. and Guston, D. and Leitschuh, C. and Oye, K. and et al.}, year={2018}, pages={S13–S39} }