@article{wang_kunz_hardesty_steevens_norberg-king_hammer_bauer_augspurger_dunn_martinez_et al._2021, title={Method Development for a Short-Term 7-Day Toxicity Test with Unionid Mussels}, volume={40}, url={http://dx.doi.org/10.1002/etc.5225}, DOI={10.1002/etc.5225}, abstractNote={AbstractThe US Environmental Protection Agency's short‐term freshwater effluent test methods include a fish (Pimephales promelas), a cladoceran (Ceriodaphnia dubia), and a green alga (Raphidocelis subcapitata). There is a recognized need for additional taxa to accompany the three standard species for effluent testing. An appropriate additional taxon is unionid mussels because mussels are widely distributed, live burrowed in sediment and filter particles from the water column for food, and exhibit high sensitivity to a variety of contaminants. Multiple studies were conducted to develop a relevant and robust short‐term test method for mussels. We first evaluated the comparative sensitivity of two mussel species (Villosa constricta and Lampsilis siliquoidea) and two standard species (P. promelas and C. dubia) using two mock effluents prepared by mixing ammonia and five metals (cadmium, copper, nickel, lead, and zinc) or a field‐collected effluent in 7‐day exposures. Both mussel species were equally or more sensitive (more than two‐fold) to effluents compared with the standard species. Next, we refined the mussel test method by first determining the best feeding rate of a commercial algal mixture for three age groups (1, 2, and 3 weeks old) of L. siliquoidea in a 7‐day feeding experiment, and then used the derived optimal feeding rates to assess the sensitivity of the three ages of juveniles in a 7‐day reference toxicant (sodium chloride [NaCl]) test. Juvenile mussels grew substantially (30%–52% length increase) when the 1‐ or 2‐week‐old mussels were fed 2 ml twice daily and the 3‐week‐old mussels were fed 3 ml twice daily. The 25% inhibition concentrations (IC25s) for NaCl were similar (314–520 mg Cl/L) among the three age groups, indicating that an age range of 1‐ to 3‐week‐old mussels can be used for a 7‐day test. Finally, using the refined test method, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7‐day NaCl test with L. siliquoidea. Eleven laboratories successfully completed the test, with more than 80% control survival and reliable growth data. The IC25s ranged from 296 to 1076 mg Cl/L, with a low (34%) coefficient of variation, indicating that the proposed method for L. siliquoidea has acceptable precision. Environ Toxicol Chem 2021;40:3392–3409. © 2021 SETAC}, number={12}, journal={ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY}, author={Wang, Ning and Kunz, James L. and Hardesty, Douglas K. and Steevens, Jeffery A. and Norberg-King, Teresa and Hammer, Edward J. and Bauer, Candice R. and Augspurger, Tom and Dunn, Suzanne and Martinez, David and et al.}, year={2021}, month={Nov}, pages={3392–3409} }
 @article{buczek_archambault_gregory cope_heilman_2020, title={Evaluation of Juvenile Freshwater Mussel Sensitivity to Multiple Forms of Florpyrauxifen-Benzyl}, volume={105}, ISSN={["1432-0800"]}, url={https://doi.org/10.1007/s00128-020-02971-1}, DOI={10.1007/s00128-020-02971-1}, number={4}, journal={BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY}, publisher={Springer Science and Business Media LLC}, author={Buczek, Sean B. and Archambault, Jennifer M. and Gregory Cope, W. and Heilman, Mark A.}, year={2020}, month={Oct}, pages={588–594} }
 @article{willis_gravelie_bishop_buczek_cope_2020, title={Managing the Invasive Cyanobacterium Lyngbya wollei in a Southeastern USA Reservoir: Evaluation of a Multi-year Treatment Program}, volume={231}, ISBN={1573-2932}, url={http://dx.doi.org/10.1007/s11270-020-04532-y}, DOI={10.1007/s11270-020-04532-y}, number={5}, journal={WATER AIR AND SOIL POLLUTION}, author={Willis, Ben E. and Gravelie, Joseph T. and Bishop, West M. and Buczek, Sean B. and Cope, W. Gregory}, year={2020} }
 @article{willis_gravelie_bishop_buczek_cope_2020, title={Managing the Invasive CyanobacteriumLyngbya wolleiin a Southeastern USA Reservoir: Evaluation of a Multi-year Treatment Program (vol 231, 196, 2020)}, volume={231}, ISSN={["1573-2932"]}, DOI={10.1007/s11270-020-04663-2}, abstractNote={The authors realized post publication that pertinent information from the Acknowledgments section was inadvertently omitted.}, number={6}, journal={WATER AIR AND SOIL POLLUTION}, author={Willis, Ben E. and Gravelie, Joseph T. and Bishop, West M. and Buczek, Sean B. and Cope, W. Gregory}, year={2020}, month={Jun} }
 @article{buczek_cope_mclaughlin_kwak_2018, title={Effects of Turbidity, Sediment, and Polyacrylamide on Native Freshwater Mussels}, volume={54}, ISSN={["1752-1688"]}, url={https://doi.org/10.1111/1752-1688.12639}, DOI={10.1111/1752-1688.12639}, abstractNote={AbstractTurbidity is a ubiquitous pollutant adversely affecting water quality and aquatic life in waterways globally. Anionic polyacrylamide (PAM) is widely used as an effective chemical flocculent to reduce suspended sediment (SS) and turbidity. However, no information exists on the toxicity of PAM‐flocculated sediments to imperiled, but ecologically important, freshwater mussels (Unionidae). Thus, we conducted acute (96 h) and chronic (24 day) laboratory tests with juvenile fatmucket (Lampsilis siliquoidea) and three exposure conditions (nonflocculated settled sediment, SS, and PAM‐flocculated settled sediment) over a range of turbidity levels (50, 250, 1,250, and 3,500 nephelometric turbidity units). Survival and sublethal endpoints of protein oxidation, adenosine triphosphate (ATP) production, and protein concentration were used as measures of toxicity. We found no effect of turbidity levels or exposure condition on mussel survival in acute or chronic tests. However, we found significant reductions in protein concentration, ATP production, and oxidized proteins in mussels acutely exposed to the SS condition, which required water movement to maintain sediment in suspension, indicating responses that are symptoms of physiological stress. Our results suggest anionic PAM applied to reduce SS may minimize adverse effects of short‐term turbidity exposure on juvenile freshwater mussels without eliciting additional lethal or sublethal toxicity.}, number={3}, journal={JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION}, author={Buczek, Sean B. and Cope, W. Gregory and McLaughlin, Richard A. and Kwak, Thomas J.}, year={2018}, month={Jun}, pages={631–643} }
 @article{buczek_cope_mclaughlin_kwak_2017, title={ACUTE TOXICITY OF POLYACRYLAMIDE FLOCCULANTS TO EARLY LIFE STAGES OF FRESHWATER MUSSELS}, volume={36}, ISSN={["1552-8618"]}, DOI={10.1002/etc.3821}, abstractNote={AbstractPolyacrylamide has become an effective tool for reducing construction‐related suspended sediment and turbidity, which are considered to have significant adverse impacts on aquatic ecosystems and are a leading cause of the degradation of North American streams and rivers. However, little is known about the effects of polyacrylamide on many freshwater organisms, and prior to the present study, no information existed on the toxicity of polyacrylamide compounds to native freshwater mussels (family Unionidae), one of the most imperiled faunal groups globally. Following standard test guidelines, we exposed juvenile mussels (test duration 96 h) and glochidia larvae (test duration 24 h) to 5 different anionic polyacrylamide compounds and 1 non‐ionic compound. Species tested included the yellow lampmussel (Lampsilis cariosa), an Atlantic Slope species that is listed as endangered in North Carolina; the Appalachian elktoe (Alasmidonta raveneliana), a federally endangered Interior Basin species; and the washboard (Megalonaias nervosa), a common Interior Basin species. We found that median lethal concentrations (LC50s) of polyacrylamide ranged from 411.7 to >1000 mg/L for glochidia and from 126.8 to >1000 mg/L for juveniles. All LC50s were orders of magnitude greater (2–3) than concentrations typically recommended for turbidity control (1–5 mg/L), regardless of their molecular weight or charge density. The results demonstrate that the polyacrylamide compounds tested were not acutely toxic to the mussel species and life stages tested, indicating minimal risk of short‐term exposure from polyacrylamide applications in the environment. However, other potential uses of polyacrylamide in the environment (e.g., wastewater treatment, paper processing, mining, algae removal) and their chronic or sublethal effects remain uncertain and warrant additional investigation. Environ Toxicol Chem 2017;36:2715–2721. Published 2017 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.}, number={10}, journal={ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY}, publisher={Wiley-Blackwell}, author={Buczek, Sean B. and Cope, W. Gregory and McLaughlin, Richard A. and Kwak, Thomas J.}, year={2017}, month={Oct}, pages={2715–2721} }