@misc{busato_gordon_chaudhari_jensen_akyol_andersen_williams_2023, title={Compositionality, sparsity, spurious heterogeneity, and other data-driven challenges for machine learning algorithms within plant microbiome studies}, volume={71}, ISSN={["1879-0356"]}, url={https://doi.org/10.1016/j.pbi.2022.102326}, DOI={10.1016/j.pbi.2022.102326}, abstractNote={The plant-associated microbiome is a key component of plant systems, contributing to their health, growth, and productivity. The application of machine learning (ML) in this field promises to help untangle the relationships involved. However, measurements of microbial communities by high-throughput sequencing pose challenges for ML. Noise from low sample sizes, soil heterogeneity, and technical factors can impact the performance of ML. Additionally, the compositional and sparse nature of these datasets can impact the predictive accuracy of ML. We review recent literature from plant studies to illustrate that these properties often go unmentioned. We expand our analysis to other fields to quantify the degree to which mitigation approaches improve the performance of ML and describe the mathematical basis for this. With the advent of accessible analytical packages for microbiome data including learning models, researchers must be familiar with the nature of their datasets.}, journal={CURRENT OPINION IN PLANT BIOLOGY}, author={Busato, Sebastiano and Gordon, Max and Chaudhari, Meenal and Jensen, Ib and Akyol, Turgut and Andersen, Stig and Williams, Cranos}, year={2023}, month={Feb} }