Works (6)
Updated: July 13th, 2023 21:20
2020 journal article
Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1)
PROTEIN JOURNAL, 39(5), 461–471.
author keywords: LRR-RLK; Post-translational modifications; N-glycosylation; LC– MS; Phylogenetic analysis
MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins / biosynthesis; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / isolation & purification; Gene Expression; Phylogeny; Protein Kinases / biosynthesis; Protein Kinases / chemistry; Protein Kinases / genetics; Protein Kinases / isolation & purification; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification
TL;DR:
This work describes expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC–MS/MS and computational sequence-based analyses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: November 16, 2020
2019 review
Plant Leucine-Rich Repeat Receptor Kinase (LRR-RK): Structure, Ligand Perception, and Activation Mechanism
[Review of ]. MOLECULES, 24(17).
author keywords: receptor kinase; LRR-RK; signaling; X-ray crystallography
MeSH headings : Leucine / chemistry; Ligands; Models, Molecular; Peptides / chemistry; Peptides / genetics; Peptides / metabolism; Plant Growth Regulators; Plant Physiological Phenomena; Plant Proteins / chemistry; Plant Proteins / physiology; Plants / genetics; Plants / metabolism; Protein Binding; Protein Conformation; Protein Interaction Domains and Motifs; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / physiology; Signal Transduction; Structure-Activity Relationship
TL;DR:
The structures of many plant LRR-RK receptors in complex with corresponding ligands have been solved by X-ray crystallography, revealing new mechanisms of ligand-induced receptor kinase activation.
(via Semantic Scholar)
open access via www.mdpi.com (publisher PDF)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: October 21, 2019
2018 journal article
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (138).
author keywords: Biochemistry; Issue 138; Insect cell; baculovirus; expression vector; secretory protein; signal peptide; GP67; hemolin; bacmid; transfection; cell culture; glycosylation; protein expression; protein crystallization
MeSH headings : Animals; Baculoviridae / genetics; Baculoviridae / metabolism; Genetic Vectors; Insecta / genetics; Plant Proteins / metabolism
TL;DR:
This newly designed modified vector system successfully produced a high yield of soluble recombinant secreted plant receptor proteins of Arabidopsis thaliana, and is an improved tool that can potentially be used for the expression of recombinantsecretory proteins in the animal kingdom.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 16, 2018
2018 journal article
The Extracellular Domain of Pollen Receptor Kinase 3 is structurally similar to the SERK family of co-receptors
SCIENTIFIC REPORTS, 8.
MeSH headings : Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Flowers / growth & development; Ovule / metabolism; Plant Proteins / metabolism; Pollen / metabolism; Pollen Tube / growth & development; Pollination; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / metabolism; Reproduction / physiology; Signal Transduction / physiology
TL;DR:
The authors' structural analyses of PRK3 have provided a template for future functional studies of the PRK family of LRR-RLK receptors in the regulation of pollen tube development, and determined the crystal structure of the extracellular domain (ecd) ofPRK3 at 2.5 Å, which resembles the SERKfamily of plant co-receptors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Differential CLE peptide perception by plant receptors implicated from structural and functional analyses of TDIF-TDR interactions
PLOS ONE, 12(4).
MeSH headings : Amino Acid Sequence; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Binding Sites; Molecular Structure; Protein Binding; Protein Kinases / metabolism; Sequence Homology, Amino Acid
TL;DR:
These studies will open different unknown avenues of TDR-TDIF signaling pathways that will enhance knowledge in this field highlighting the receptor ligand interaction, receptor activation, signaling network, modes of action and will serve as a structure function relationship model between the ligand and the receptor for various similar leucine-rich repeat receptor-like kinases (LRR-RLKs).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
X-ray crystallographic studies of the extracellular domain of the first plant ATP receptor, DORN1, and the orthologous protein from Camelina sativa
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 72, 782–787.
author keywords: plant ATP receptor; DORN1; lectin receptor kinase I.9; glycosylation; Arabidopsis thaliana; Camelina sativa
MeSH headings : Adenosine Triphosphate / chemistry; Adenosine Triphosphate / metabolism; Amino Acid Sequence; Arabidopsis / chemistry; Arabidopsis / metabolism; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Baculoviridae / genetics; Baculoviridae / metabolism; Brassicaceae / chemistry; Brassicaceae / metabolism; Cloning, Molecular; Crystallization; Crystallography, X-Ray; Gene Expression; Glycosylation; Plasmids / chemistry; Plasmids / metabolism; Protein Kinases / chemistry; Protein Kinases / genetics; Protein Kinases / metabolism; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; X-Ray Diffraction
TL;DR:
The extracellular domain of the A. thaliana ATP receptor DORN1 has been expressed and purified; a protein orthologous to Dorn1 from C. sativa has been crystallized and its X-ray diffraction data have been collected.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018