Sean D. Stowe

Works (6)

Updated: July 23rd, 2023 19:25

2019 journal article

The Solution Structures and Interaction of SinR and SinI: Elucidating the Mechanism of Action of the Master Regulator Switch for Biofilm Formation in Bacillus subtilis

JOURNAL OF MOLECULAR BIOLOGY, 432(2), 343–357.

By: M. Milton *, G. Draughn n, B. Bobay n, S. Stowe n, A. Olson n, E. Feldmann n, R. Thompson*, K. Myers n ...

author keywords: Biofilms; Bacillus subtilis; Transcriptional regulation; NMR solution structure
MeSH headings : Amino Acid Sequence / genetics; Bacillus subtilis / genetics; Bacillus subtilis / ultrastructure; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / ultrastructure; Biofilms / growth & development; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / ultrastructure; Gene Expression Regulation, Bacterial / genetics; Molecular Docking Simulation; Mutation / genetics; Protein Binding / genetics; Protein Conformation
TL;DR: This work presents the first full-length structural model of tetrameric SinR using a hybrid approach combining high-resolution solution NMR, chemical crosslinking, mass spectrometry, and molecular docking, and provides an evidence-based mechanism that confirms how disruption of the SinR tetramer by SinI regulates gene expression. (via Semantic Scholar)
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Source: Web Of Science
Added: February 27, 2020

2015 journal article

Membrane-Permeabilizing Activity of Reverse-Amide 2-Aminoimidazole Antibiofilm Agents Against Acinetobacter baumannii

CURRENT DRUG DELIVERY, 12(2), 223–230.

By: S. Stowe*, R. Thompson*, L. Peng*, Z. Su*, M. Blackledge*, G. Draughn, W. Coe, E. Johannes* ...

author keywords: 2-aminoimidazole; Acinetobacter baumannii; biofilm; membrane permeabilization; reverse amide; SEM
MeSH headings : Acinetobacter baumannii / drug effects; Amides / chemistry; Anti-Bacterial Agents / chemistry; Anti-Bacterial Agents / pharmacology; Biofilms / drug effects; Cell Membrane Permeability / drug effects; Cells, Cultured; Cross Infection; Imidazoles / chemistry; Imidazoles / pharmacology; Microbial Sensitivity Tests
TL;DR: A model is proposed that describes how the structure of RA-2AI allows it to insert itself into and disrupt the fluidity of the membrane, creating an opportunity for increased molecular permeability. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2013 journal article

Chemical shift assignments and secondary structure prediction of the master biofilm regulator, SinR, from Bacillus subtilis

BIOMOLECULAR NMR ASSIGNMENTS, 8(1), 155–158.

By: S. Stowe n, A. Olson n, R. Losick * & J. Cavanagh n

author keywords: NMR; SinR; Bacillus subtilis; Biofilm regulation
MeSH headings : Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Biofilms; Nuclear Magnetic Resonance, Biomolecular; Protein Structure, Secondary
TL;DR: The full-length backbone and side chain chemical shift values in addition to the experimentally derived secondary structure predictions are reported as the first step towards directly studying the complex interaction dynamics between SinR and SinI. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2012 journal article

Evaluation of the toxicity of 2-aminoimidazole antibiofilm agents using both cellular and model organism systems

DRUG AND CHEMICAL TOXICOLOGY, 35(3), 310–315.

By: S. Stowe n, A. Tucker*, R. Thompson*, A. Piper*, J. Richards*, S. Rogers*, L. Mathies n, C. Melander*, J. Cavanagh*

author keywords: 2-aminoimidazole; adjuvant; biofilm; Caenorhabditis elegans; toxicity; Toxicity of 2-aminoimidazole; antibiofilm agents
MeSH headings : Adjuvants, Pharmaceutic / toxicity; Animals; Anti-Bacterial Agents / pharmacology; Anti-Bacterial Agents / toxicity; Biofilms / drug effects; Caenorhabditis elegans; Cell Line; Dose-Response Relationship, Drug; Environmental Exposure; Fertility / drug effects; Humans; Imidazoles / pharmacology; Imidazoles / toxicity; Keratinocytes / metabolism; Tetrazolium Salts; Thiazoles; Toxicity Tests
TL;DR: The most effective antibiofilm compound had significant effects when used in conjunction with several standard antibiotics against resistant bacteria and was found to maintain the same low level of toxicity as when acting alone, bolstering its candidacy for further testing as an adjuvant. (via Semantic Scholar)
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Added: August 6, 2018

2012 journal article

Identification of BfmR, a Response Regulator Involved in Biofilm Development, as a Target for a 2-Aminoimidazole-Based Antibiofilm Agent

BIOCHEMISTRY, 51(49), 9776–9778.

By: R. Thompson n, B. Bobay n, S. Stowe n, A. Olson n, L. Peng n, Z. Su n, L. Actis *, C. Melander n, J. Cavanagh n

MeSH headings : Acinetobacter baumannii / drug effects; Acinetobacter baumannii / growth & development; Bacterial Proteins / chemistry; Bacterial Proteins / physiology; Biofilms / drug effects; Imidazoles / pharmacology; Microscopy, Confocal; Models, Molecular
TL;DR: The 2AI compound studied here represents a unique small molecule scaffold that targets bacterial response regulators and is cell permeable, while pull-down assays identified BfmR, a response regulator that is the master controller of biofilm formation, as a target for this compound. (via Semantic Scholar)
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Added: August 6, 2018

2011 review

Anti-biofilm compounds derived from marine sponges

[Review of ]. Marine Drugs, 9(10), 2010–2035.

By: S. Stowe, J. Richards, A. Tucker, R. Thompson, C. Melander & J. Cavanagh

Source: NC State University Libraries
Added: August 6, 2018

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