@article{fan_foster_zhao_mukherjee_shrestha_parsons_kathariou_2022, title={Genomic Analysis Reveals That Isolation Temperature on Selective Media Introduces Genetic Variation in Campylobacter jejuni from Bovine Feces}, volume={11}, ISSN={["2076-0817"]}, DOI={10.3390/pathogens11060678}, abstractNote={Campylobacter jejuni is commonly isolated on selective media following incubation at 37 °C or 42 °C, but the impact of these temperatures on genome variation remains unclear. Previously, Campylobacter selective enrichments from the feces of steers before and after ceftiofur treatment were plated on selective agar media and incubated at either 37 °C or 42 °C. Here, we analyzed the whole genome sequence of C. jejuni strains of the same multilocus sequence typing (MLST)-based sequence type (ST) and isolated from the same sample upon incubation at both temperatures. Four such strain pairs (one ST8221 and three ST8567) were analyzed using core genome and whole genome MLST (cgMLST, wgMLST). Among the 1970 wgMLST loci, 7–25 varied within each pair. In all but one of the pairs more (1.7–8.5 fold) new alleles were found at 42 °C. Most frameshift, nonsense, or start-loss mutations were also found at 42 °C. Variable loci CAMP0575, CAMP0912, and CAMP0913 in both STs may regularly respond to different temperatures. Furthermore, frameshifts in four variable loci in ST8567 occurred at multiple time points, suggesting a persistent impact of temperature. These findings suggest that the temperature of isolation may impact the sequence of several loci in C. jejuni from cattle.}, number={6}, journal={PATHOGENS}, author={Fan, Sicun and Foster, Derek and Zhao, Shaohua and Mukherjee, Sampa and Shrestha, Yesha and Parsons, Cameron and Kathariou, Sophia}, year={2022}, month={Jun} }
 @article{fan_foster_miller_osborne_kathariou_2021, title={Impact of Ceftiofur Administration in Steers on the Prevalence and Antimicrobial Resistance of Campylobacter spp.}, volume={9}, ISSN={["2076-2607"]}, DOI={10.3390/microorganisms9020318}, abstractNote={Bacterial resistance to ceftiofur raises health concerns due to ceftiofur’s extensive veterinary usage and structural similarity with the human antibiotic ceftriaxone. Ceftiofur crystalline-free acid (CCFA) and ceftiofur hydrochloride (CHCL) are ceftiofur types used therapeutically in cattle, but their potential impacts on Campylobacter prevalence and antimicrobial resistance remain unclear. In this study two groups of steers were each treated with CCFA or CHCL. In vivo active drug concentrations were measured and fecal samples were analyzed for Campylobacter for up to 42 days post-treatment. Following administration, the colonic concentration of ceftiofur initially increased then dropped to pre-treatment levels by day 8. The estimated prevalence of Campylobacter spp. was significantly (p = 0.0009) higher during the first week after CCFA treatment than after CHCL treatment (81.3% vs. 45.2%). Campylobacter jejuni predominated overall, with other Campylobacter spp. mainly identified in the first week after CCFA treatment. No treatment impacts were noted on ceftiofur minimum inhibitory concentration (MIC) for C. jejuni (10–20 μg/mL). More C. jejuni genotypes were detected in CCFA-treated than CHCL-treated steers. These findings suggest that ceftiofur did not significantly impact Campylobacter prevalence or ceftiofur MIC. However, CHCL may be preferable due to the lower likelihood of temporary increases in Campylobacter prevalence.}, number={2}, journal={MICROORGANISMS}, author={Fan, Sicun and Foster, Derek and Miller, William G. and Osborne, Jason and Kathariou, Sophia}, year={2021}, month={Feb} }
 @article{fan_breidt_price_perez-diaz_2017, title={Survival and Growth of Probiotic Lactic Acid Bacteria in Refrigerated Pickle Products}, volume={82}, ISSN={["1750-3841"]}, DOI={10.1111/1750-3841.13579}, abstractNote={Abstract}, number={1}, journal={JOURNAL OF FOOD SCIENCE}, author={Fan, Sicun and Breidt, Fred and Price, Robert and Perez-Diaz, Ilenys}, year={2017}, month={Jan}, pages={167–173} }