Sean G. Simpson

Works (8)

Updated: July 17th, 2023 21:18

2022 journal article

Allogeneic and xenogeneic lymphoid reconstitution in a RAG2(-/-)IL2RG(y/-) severe combined immunodeficient pig: A preclinical model for intrauterine hematopoietic transplantation

FRONTIERS IN VETERINARY SCIENCE, 9.

By: R. Sper  n, J. Proctor n, O. Lascina n, L. Guo  n, K. Polkoff n, T. Kaeser n , S. Simpson n, L. Borst n  ...

author keywords: severe combined immunodeficiency; pig; xenotransplantation; allotransplantation; hematopoietic; RAG2; IL2RG; transgenic
TL;DR: The data shows that the pig thymus provides a microenvironment conducive to engraftment, survival and development of human T-cells and provides evidence that the developing T-cell compartment can be populated to a significant extent by human cells in large animals. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 14, 2022

2022 journal article

LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis

SCIENTIFIC REPORTS, 12(1).

By: K. Polkoff n, N. Gupta  n, A. Green n, Y. Murphy n, J. Chung n, K. Gleason n, S. Simpson*, D. Walker n, B. Collins n, J. Piedrahita n 

MeSH headings : Animals; Animals, Genetically Modified; Biomarkers / metabolism; Hair Follicle / metabolism; Humans; Morphogenesis; Receptors, G-Protein-Coupled / genetics; Receptors, G-Protein-Coupled / metabolism; Skin; Stem Cells / metabolism; Swine
TL;DR: It is demonstrated that LGR5 is a marker of hair follicle stem cells across species in homeostasis and development and the strong similarities and important differences in expression patterns, gene expression profiles, and developmental processes between species are reported. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 20, 2022

2021 journal article

Patch grafting, strategies for transplantation of organoids into solid organs such as liver

BIOMATERIALS, 277.

By: W. Zhang *, G. Lanzoni *, H. Hani *, D. Overi *, V. Cardinale *, S. Simpson n, W. Pitman*, A. Allen* ...

author keywords: Liver; Biliary tree; Stem/progenitors; Cell therapies; Grafting; Matrix-metalloproteinases (MMPs); Hyaluronans
MeSH headings : Animals; Cell Differentiation; Hepatocytes; Liver; Organoids; Stem Cells; Swine
TL;DR: RNA-seq analyses revealed that engraftment correlated with expression of matrix-metalloproteinases (MMPs), especially secreted isoforms that were found expressed strongly by organoids, less so by MSCs, and minimally, if at all, by adult cells. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 4, 2021

2020 journal article

In Vitro Validation of Transgene Expression in Gene-Edited Pias Using CRISPR Transcriptional Activators

CRISPR JOURNAL, 3(5), 409–418.

By: K. Polkoff n, J. Chung n, S. Simpson*, K. Gleason n & J. Piedrahita n 

MeSH headings : Animals; Animals, Genetically Modified / genetics; CRISPR-Associated Protein 9 / genetics; CRISPR-Associated Protein 9 / metabolism; Cells, Cultured; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Editing / methods; Gene Expression; Nuclear Transfer Techniques; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism; Swine; Trans-Activators / genetics; Trans-Activators / metabolism; Transgenes
TL;DR: It is shown that CRISPR-dCas9 transcriptional activators can be used to validate functional transgene insertion in nonexpressing easily cultured cells such as fibroblasts and is a tool that could be used across disciplines and animal species to save time and resources by verifying expected outcomes of gene edits before generating live animals. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: November 24, 2020

2018 journal article

High mobility group A2 (HMGA2) deficiency in pigs leads to dwarfism, abnormal fetal resource allocation, and cryptorchidism

Proceedings of the National Academy of Sciences, 115(21), 5420–5425.

By: J. Chung n, X. Zhang n, B. Collins n, R. Sper  n, K. Gleason n, S. Simpson n, S. Koh  n, J. Sommer n ...

author keywords: HMGA2; dwarfism; swine; gene editing; organ size
MeSH headings : Animals; Cryptorchidism / etiology; Cryptorchidism / pathology; Dwarfism / etiology; Dwarfism / pathology; Female; Fetal Diseases / etiology; Fetal Diseases / pathology; Genotype; HMGA2 Protein / deficiency; HMGA2 Protein / genetics; Litter Size; Male; Nuclear Transfer Techniques / veterinary; Pregnancy; Reproduction; Swine; Swine Diseases / etiology; Swine Diseases / pathology
TL;DR: The results show that the effect of HMGA2 with respect to growth regulation is highly conserved among mammals and opens up the possibility of regulating body and organ size in a variety of mammalian species including food and companion animals. (via Semantic Scholar)
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Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2016 journal article

Effect of Plant Species, Fertilizer Acidity/Basicity, and Fertilizer Concentration on pH of Soilless Root Substrate

HORTSCIENCE, 51(12), 1596–1601.

By: K. Jeong n, P. Nelson n, C. Niedziela  n & D. Dickey n

author keywords: alkalinity; electrical conductivity; root media
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Cloning, characterization, and expression analysis of the pig (Sus scrofa) C1q tumor necrosis factor-related protein-5 gene

Molecular Vision, 18(12-14), 92–102.

By: J. Sommer, V. Chavali, S. Simpson, R. Ayyagari & R. Petters

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

Transgenic Stra8-EYFP pigs: a model for developing male germ cell technologies

TRANSGENIC RESEARCH, 21(2), 383–392.

By: J. Sommer n, L. Jackson n, S. Simpson n, E. Collins n, J. Piedrahita n  & R. Petters n

author keywords: Pig; Transgenic; Stra8; Spermatogonia; Marker gene
MeSH headings : Adaptor Proteins, Signal Transducing; Animals; Animals, Genetically Modified / genetics; Animals, Genetically Modified / metabolism; Blotting, Western; Cells, Cultured; Cloning, Molecular; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / metabolism; Gene Expression Regulation, Developmental; Genes, Reporter; Germ Cells / cytology; Germ Cells / metabolism; Homeodomain Proteins / genetics; Homeodomain Proteins / metabolism; Immunohistochemistry; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Male; Mice; Microscopy, Fluorescence / methods; Mitochondria / genetics; Mitochondria / metabolism; Nuclear Transfer Techniques; Plasmids / genetics; Plasmids / metabolism; Promoter Regions, Genetic; Proteins / genetics; Proteins / metabolism; Swine; Testis / cytology; Testis / metabolism; Transfection; Transgenes; Tretinoin / pharmacology
TL;DR: Transgenic pigs expressing mitochondrial localized enhanced yellow fluorescent protein (EYFP-mito) under control of the germ cell specific Stimulated by Retinoic Acid 8 (Stra8) promoter indicates that these pigs will be useful by providing labelled cells for use in such technologies such as germ cell transplantation and in vitro spermatogenic studies. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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