Sean G. Simpson

Works (10)

Updated: January 15th, 2026 14:32

2025 article

A preclinical pig model of Angelman syndrome mirrors the early developmental trajectory of the human condition

Myers, L. S., Christian, S. G., Simpson, S., Sper, R., Taylor, C., Montes, L., … Dindot, S. V. (2025, July 21). Proceedings of the National Academy of Sciences, Vol. 122.

By: L. Myers *, S. Christian *, S. Simpson n, R. Sper  n, C. Taylor*, L. Montes*, T. Jepp *, D. Ramos* ...

author keywords: angelman syndrome; UBE3A; Susscrofa; pig; CRISPR/Cas9
MeSH headings : Animals; Angelman Syndrome / genetics; Angelman Syndrome / physiopathology; Angelman Syndrome / pathology; Disease Models, Animal; Ubiquitin-Protein Ligases / genetics; Ubiquitin-Protein Ligases / metabolism; Humans; Swine; Brain / growth & development; Brain / pathology; Animals, Newborn; Male; Behavior, Animal; Female
topics (OpenAlex): Genetic Syndromes and Imprinting; Growth Hormone and Insulin-like Growth Factors
Sources: Web Of Science, NC State University Libraries
Added: August 18, 2025

2025 article

A preclinical pig model of Angelman syndrome mirrors the early developmental trajectory of the human condition

Myers, L. S., Christian, S. G., Simpson, S., Sper, R., Taylor, C., Montes, L., … Dindot, S. V. (2025, March 11). (Vol. 3). Vol. 3.

By: L. Myers *, S. Christian *, S. Simpson n, R. Sper  n, C. Taylor*, L. Montes *, T. Jepp *, D. Ramos* ...

topics (OpenAlex): Genetic Syndromes and Imprinting
Sources: NC State University Libraries, NC State University Libraries
Added: January 13, 2026

2022 article

Allogeneic and xenogeneic lymphoid reconstitution in a RAG2−/−IL2RGy/− severe combined immunodeficient pig: A preclinical model for intrauterine hematopoietic transplantation

Sper, R. B., Proctor, J., Lascina, O., Guo, L., Polkoff, K., Kaeser, T., … Piedrahita, J. A. (2022, October 14). Frontiers in Veterinary Science, Vol. 9.

By: R. Sper  n, J. Proctor n, O. Lascina n, L. Guo  n, K. Polkoff n, T. Kaeser n , S. Simpson n, L. Borst n  ...

author keywords: severe combined immunodeficiency; pig; xenotransplantation; allotransplantation; hematopoietic; RAG2; IL2RG; transgenic
topics (OpenAlex): Xenotransplantation and immune response; Hematopoietic Stem Cell Transplantation; Diabetes and associated disorders
TL;DR: The data shows that the pig thymus provides a microenvironment conducive to engraftment, survival and development of human T-cells and provides evidence that the developing T-cell compartment can be populated to a significant extent by human cells in large animals. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 14, 2022

2022 article

LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis

Polkoff, K. M., Gupta, N. K., Green, A. J., Murphy, Y., Chung, J., Gleason, K. L., … Piedrahita, J. A. (2022, June 1). Scientific Reports, Vol. 12.

By: K. Polkoff n, N. Gupta  n, A. Green n, Y. Murphy n, J. Chung n, K. Gleason n, S. Simpson*, D. Walker n, B. Collins n, J. Piedrahita n 

MeSH headings : Animals; Animals, Genetically Modified; Biomarkers / metabolism; Hair Follicle / metabolism; Humans; Morphogenesis; Receptors, G-Protein-Coupled / genetics; Receptors, G-Protein-Coupled / metabolism; Skin; Stem Cells / metabolism; Swine
topics (OpenAlex): Hair Growth and Disorders; Cancer and Skin Lesions; Skin and Cellular Biology Research
TL;DR: It is demonstrated that LGR5 is a marker of hair follicle stem cells across species in homeostasis and development and the strong similarities and important differences in expression patterns, gene expression profiles, and developmental processes between species are reported. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 20, 2022

2021 journal article

Patch grafting, strategies for transplantation of organoids into solid organs such as liver

Biomaterials, 277, 121067.

By: W. Zhang *, G. Lanzoni *, H. Hani *, D. Overi *, V. Cardinale *, S. Simpson n, W. Pitman*, A. Allen* ...

author keywords: Liver; Biliary tree; Stem/progenitors; Cell therapies; Grafting; Matrix-metalloproteinases (MMPs); Hyaluronans
MeSH headings : Animals; Cell Differentiation; Hepatocytes; Liver; Organoids; Stem Cells; Swine
topics (OpenAlex): Liver physiology and pathology; Tissue Engineering and Regenerative Medicine; Pancreatic function and diabetes
TL;DR: RNA-seq analyses revealed that engraftment correlated with expression of matrix-metalloproteinases (MMPs), especially secreted isoforms that were found expressed strongly by organoids, less so by MSCs, and minimally, if at all, by adult cells. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 4, 2021

2020 article

In Vitro Validation of Transgene Expression in Gene-Edited Pigs Using CRISPR Transcriptional Activators

Polkoff, K. M., Chung, J., Simpson, S. G., Gleason, K., & Piedrahita, J. A. (2020, October 1). The CRISPR Journal, Vol. 3, pp. 409–418.

By: K. Polkoff n, J. Chung n, S. Simpson*, K. Gleason n & J. Piedrahita n 

MeSH headings : Animals; Animals, Genetically Modified / genetics; CRISPR-Associated Protein 9 / genetics; CRISPR-Associated Protein 9 / metabolism; Cells, Cultured; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Editing / methods; Gene Expression; Nuclear Transfer Techniques; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism; Swine; Trans-Activators / genetics; Trans-Activators / metabolism; Transgenes
topics (OpenAlex): CRISPR and Genetic Engineering; Animal Genetics and Reproduction; Virus-based gene therapy research
TL;DR: It is shown that CRISPR-dCas9 transcriptional activators can be used to validate functional transgene insertion in nonexpressing easily cultured cells such as fibroblasts and is a tool that could be used across disciplines and animal species to save time and resources by verifying expected outcomes of gene edits before generating live animals. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: November 24, 2020

2018 journal article

High mobility group A2 (HMGA2) deficiency in pigs leads to dwarfism, abnormal fetal resource allocation, and cryptorchidism

Proceedings of the National Academy of Sciences, 115(21), 5420–5425.

By: J. Chung n, X. Zhang n, B. Collins n, R. Sper  n, K. Gleason n, S. Simpson n, S. Koh  n, J. Sommer n ...

author keywords: HMGA2; dwarfism; swine; gene editing; organ size
MeSH headings : Animals; Cryptorchidism / etiology; Cryptorchidism / pathology; Dwarfism / etiology; Dwarfism / pathology; Female; Fetal Diseases / etiology; Fetal Diseases / pathology; Genotype; HMGA2 Protein / deficiency; HMGA2 Protein / genetics; Litter Size; Male; Nuclear Transfer Techniques / veterinary; Pregnancy; Reproduction; Swine; Swine Diseases / etiology; Swine Diseases / pathology
topics (OpenAlex): Animal Genetics and Reproduction; Cancer-related molecular mechanisms research; Genetic and phenotypic traits in livestock
TL;DR: The results show that the effect of HMGA2 with respect to growth regulation is highly conserved among mammals and opens up the possibility of regulating body and organ size in a variety of mammalian species including food and companion animals. (via Semantic Scholar)
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Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2016 article

Effect of Plant Species, Fertilizer Acidity/Basicity, and Fertilizer Concentration on pH of Soilless Root Substrate

Jeong, K. Y., Nelson, P. V., Niedziela, C. E., & Dickey, D. A. (2016, December 1). HortScience, Vol. 51, pp. 1596–1601.

By: K. Jeong n, P. Nelson n, C. Niedziela  n & D. Dickey n

author keywords: alkalinity; electrical conductivity; root media
topics (OpenAlex): Composting and Vermicomposting Techniques; Seedling growth and survival studies; Plant Growth Enhancement Techniques
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Cloning, characterization, and expression analysis of the pig (Sus scrofa) C1q tumor necrosis factor-related protein-5 gene

Molecular Vision, 18(12-14), 92–102.

By: J. Sommer, V. Chavali, S. Simpson, R. Ayyagari & R. Petters

Source: NC State University Libraries
Added: August 6, 2018

2011 article

Transgenic Stra8-EYFP pigs: a model for developing male germ cell technologies

Sommer, J. R., Jackson, L. R., Simpson, S. G., Collins, E. B., Piedrahita, J. A., & Petters, R. M. (2011, August 8). Transgenic Research, Vol. 21, pp. 383–392.

By: J. Sommer n, L. Jackson n, S. Simpson n, E. Collins n, J. Piedrahita n  & R. Petters n

author keywords: Pig; Transgenic; Stra8; Spermatogonia; Marker gene
MeSH headings : Adaptor Proteins, Signal Transducing; Animals; Animals, Genetically Modified / genetics; Animals, Genetically Modified / metabolism; Blotting, Western; Cells, Cultured; Cloning, Molecular; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / metabolism; Gene Expression Regulation, Developmental; Genes, Reporter; Germ Cells / cytology; Germ Cells / metabolism; Homeodomain Proteins / genetics; Homeodomain Proteins / metabolism; Immunohistochemistry; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Male; Mice; Microscopy, Fluorescence / methods; Mitochondria / genetics; Mitochondria / metabolism; Nuclear Transfer Techniques; Plasmids / genetics; Plasmids / metabolism; Promoter Regions, Genetic; Proteins / genetics; Proteins / metabolism; Swine; Testis / cytology; Testis / metabolism; Transfection; Transgenes; Tretinoin / pharmacology
topics (OpenAlex): Animal Genetics and Reproduction; CRISPR and Genetic Engineering; Virus-based gene therapy research
TL;DR: Transgenic pigs expressing mitochondrial localized enhanced yellow fluorescent protein (EYFP-mito) under control of the germ cell specific Stimulated by Retinoic Acid 8 (Stra8) promoter indicates that these pigs will be useful by providing labelled cells for use in such technologies such as germ cell transplantation and in vitro spermatogenic studies. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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