@article{bodle_hamouda_cai_williams_bernacki_loboa_2019, title={Primary Cilia Exhibit Mechanosensitivity to Cyclic Tensile Strain and Lineage-Dependent Expression in Adipose-Derived Stem Cells}, volume={9}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-019-43351-y}, abstractNote={Abstract}, journal={SCIENTIFIC REPORTS}, author={Bodle, Josephine and Hamouda, Mehdi S. and Cai, Shaobo and Williams, Ramey B. and Bernacki, Susan H. and Loboa, Elizabeth G.}, year={2019}, month={May} }
 @article{johnson_macpherson_smith_block_keyton_2016, title={Facilitating Teamwork in Adolescent and Young Adult Oncology}, volume={12}, ISSN={["1935-469X"]}, DOI={10.1200/jop.2016.013870}, abstractNote={ A case of a young adult patient in the days immediately after a cancer diagnosis illustrates the critical importance of three interrelated core coordinating mechanisms—closed-loop communication, shared mental models, and mutual trust—of teamwork in an adolescent and young adult multidisciplinary oncology team. The case illustrates both the opportunities to increase team member coordination and the problems that can occur when coordination breaks down. A model for teamwork is presented, which highlights the relationships among these coordinating mechanisms and demonstrates how balance among them works to optimize team function and patient care. Implications for clinical practice and research suggested by the case are presented. }, number={11}, journal={JOURNAL OF ONCOLOGY PRACTICE}, author={Johnson, Rebecca H. and Macpherson, Catherine Fiona and Smith, Ashley W. and Block, Rebecca G. and Keyton, Joann}, year={2016}, month={Nov}, pages={1067-+} }
 @article{tambe_di_zhang_bernacki_el-shafei_king_2015, title={Novel genipin-collagen immobilization of polylactic acid (PLA) fibers for use as tissue engineering scaffolds}, volume={103}, ISSN={["1552-4981"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84937440015&partnerID=MN8TOARS}, DOI={10.1002/jbm.b.33285}, abstractNote={Abstract}, number={6}, journal={JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS}, author={Tambe, Nisarg and Di, Jin and Zhang, Ze and Bernacki, Susan and El-Shafei, Ahmed and King, Martin W.}, year={2015}, month={Aug}, pages={1188–1197} }
 @article{bodle_teeter_hluck_hardin_bernacki_loboa_2014, title={Age-Related Effects on the Potency of Human Adipose-Derived Stem Cells: Creation and Evaluation of Superlots and Implications for Musculoskeletal Tissue Engineering Applications}, volume={20}, ISSN={1937-3384 1937-3392}, url={http://dx.doi.org/10.1089/ten.TEC.2013.0683}, DOI={10.1089/ten.tec.2013.0683}, abstractNote={Human adipose-derived stem cells (hASC) are now a prevalent source of adult stem cells for studies in tissue engineering and regenerative medicine. However, researchers utilizing hASC in their investigations often encounter high levels of donor-to-donor variability in hASC differentiation potential. Because of this, conducting studies with this primary cell type can require extensive resources to generate statistically significant data. We present a method to generate pooled donor cell populations, termed "superlots," containing cell populations derived from four to five age-clustered donors. The goal of generating these superlots was to 1) increase experimental throughput, 2) to utilize assay resources more efficiently, and 3) to begin to establish global hASC differentiation behaviors that may be associated with donor age. With our superlot approach, we have validated that pooled donor cell populations exhibit proliferative activity representing the combined behavior of each individual donor cell line. Further, the superlots also exhibit differentiation levels roughly approximating the average combined differentiation levels of each individual donor cell line. We established that high donor-to-donor variability exists between the pre-, peri-, and postmenopausal age groupings and that proliferation and differentiation characteristics can vary widely, independent of age. Interestingly, we did observe that cell lines derived from postmenopausal donors demonstrated a relatively high proclivity for osteogenic differentiation and a relatively lowered proclivity for adipogenic differentiation as compared with cells derived from pre- and perimenopausal donors. In general, superlots effectively represented the average differentiation behavior of each of their contributing cell populations and could provide a powerful tool for increasing experimental throughput to more efficiently utilize resources when studying hASC differentiation.}, number={12}, journal={Tissue Engineering Part C: Methods}, publisher={Mary Ann Liebert Inc}, author={Bodle, Josephine C. and Teeter, Stephanie D. and Hluck, Brandon H. and Hardin, Joseph W. and Bernacki, Susan H. and Loboa, Elizabeth G.}, year={2014}, month={Dec}, pages={972–983} }
 @article{springer_harrysson_marcellin-little_bernacki_2014, title={In vitro dermal and epidermal cellular response to titanium alloy implants fabricated with electron beam melting}, volume={36}, ISSN={["1873-4030"]}, DOI={10.1016/j.medengphy.2014.07.004}, abstractNote={Transdermal osseointegrated prostheses (TOPs) are emerging as an alternative to socket prostheses. Electron beam melting (EBM) is a promising additive manufacturing technology for manufacture of custom, freeform titanium alloy (Ti6Al4V) implants. Skin ongrowth for infection resistance and mechanical stability are critically important to the success of TOP, which can be influenced by material composition and surface characteristics. We assessed viability and proliferation of normal human epidermal keratinocytes (NHEK) and normal human dermal fibroblasts (NHDF) on several Ti6Al4V surfaces: solid polished commercial, solid polished EBM, solid unpolished EBM and porous unpolished EBM. Cell proliferation was evaluated at days 2 and 7 using alamarBlue(®) and cell viability was analyzed with a fluorescence-based live-dead assay after 1 week. NHDF and NHEK were viable and proliferated on all Ti6Al4V surfaces. NHDF proliferation was highest on commercial and EBM polished surfaces. NHEK was highest on commercial polished surfaces. All EBM Ti6Al4V discs exhibited an acceptable biocompatibility profile compared to solid Ti6Al4V discs from a commercial source for dermal and epidermal cells. EBM may be considered as an option for fabrication of custom transdermal implants.}, number={10}, journal={MEDICAL ENGINEERING & PHYSICS}, author={Springer, Jessica Collins and Harrysson, Ola L. A. and Marcellin-Little, Denis J. and Bernacki, Susan H.}, year={2014}, month={Oct}, pages={1367–1372} }
 @article{canbolat_tang_bernacki_pourdeyhimi_khan_2011, title={Mammalian Cell Viability in Electrospun Composite Nanofiber Structures}, volume={11}, ISSN={["1616-5195"]}, DOI={10.1002/mabi.201100108}, abstractNote={Abstract}, number={10}, journal={MACROMOLECULAR BIOSCIENCE}, author={Canbolat, Mehmet Fatih and Tang, Christina and Bernacki, Susan H. and Pourdeyhimi, Behnam and Khan, Saad}, year={2011}, month={Oct}, pages={1346–1356} }
 @article{mccullen_zhan_onorato_bernacki_loboa_2010, title={Effect of Varied Ionic Calcium on Human Adipose-Derived Stem Cell Mineralization}, volume={16}, ISSN={["1937-3341"]}, DOI={10.1089/ten.tea.2009.0691}, abstractNote={Human adipose-derived stem cells (hASCs) are a relatively abundant and accessible stem cell source with multilineage differentiation capability and have great potential for bone tissue engineering applications. The success of bone tissue engineering is intimately linked with the production of a mineralized matrix that mimics the natural mineral present within native bone. In this study, we examined the effects of ionic calcium levels of 1.8 (normal concentration in cell culture medium), 8, and 16 mM on hASCs seeded in both two-dimensional monolayer and three-dimensional electrospun scaffolds and cultured in either complete growth medium (CGM) or osteogenic differentiation medium (ODM). The impact of calcium supplementation on hASC viability, proliferation, and mineral deposition was determined. hASCs remained viable for all experimental treatments. hASC proliferation increased with the addition of 8 mM Ca(2+) CGM, but decreased for the 16 mM Ca(2+) CGM treatment. Materials deposited by hASCs were analyzed using four techniques: (1) histological staining with Alizarin Red S, (2) calcium quantification, (3) Fourier transform infrared spectroscopy, and (4) wide-angle X-ray diffraction. Mineral deposition was significantly enhanced under both growth and osteogenic medium conditions by increasing extracellular Ca(2+). The greatest mineral deposition occurred in the ODM 8 mM Ca(2+) treatment group. Fourier transform infrared spectroscopy analysis indicated that elevated calcium concentrations of 8 mM Ca(2+) significantly increased both PO(4) amount and PO(4) to protein ratio for ODM. X-ray diffraction indicated that mineral produced with elevated Ca(2+) in both CGM and ODM had a crystalline structure characteristic of hydroxyapatite. Ionic calcium should be considered a potent regulator in hASC mineralization and could serve as a potential treatment for inducing prompt ossification of hASC-seeded scaffolds for bone tissue engineering prior to implantation.}, number={6}, journal={TISSUE ENGINEERING PART A}, author={McCullen, Seth D. and Zhan, Jackie and Onorato, Maureen L. and Bernacki, Susan H. and Loboa, Elizabeth G.}, year={2010}, month={Jun}, pages={1971–1981} }
 @article{marvel_okrasinski_bernacki_loboa_dayton_2010, title={The Development and Validation of a LIPUS System With Preliminary Observations of Ultrasonic Effects on Human Adult Stem Cells}, volume={57}, ISSN={["1525-8955"]}, DOI={10.1109/tuffc.2010.1645}, abstractNote={To study the potential effects of low-intensity pulsed ultrasound (LIPUS) on cell response in vitro, the ability to alter LIPUS parameters is required. However, commercial LIPUS systems have very little control over parameter selection. In this study, a custom LIPUS system was designed and validated by exploring the effects of using different pulse repetition frequency (PRF) parameters on human adipose derived adult stem cells (hASCs) and bone marrow derived mesenchymal stem cells (hMSCs), two common stem cell sources for creating bone constructs in vitro. Changing the PRF was found to affect cellular response to LIPUS stimulation for both cell types. Proliferation of LIPUS-stimulated cells was found to decrease for hASCs by d 7 for all three groups compared with unstimulated control cells (P = 0.008, 0.011, 0.014 for 1 Hz, 100 Hz and 1 kHz PRF, respectively) and for hMSCs by d 14 (donor 1: P = 0.0005, 0.0002, 0.0003; donor 2: P = 0.0003, 0.0002, 0.0001; for PRFs of 1 Hz, 100 Hz, and 1 kHz, respectively). Additionally, LIPUS was shown to strongly accelerate osteogenic differentiation of hASCs based on amount of calcium accretion normalized by total DNA (P = 0.003, 0.001, 0.003, and 0.032 between control/100 Hz, control/1 kHz, 1 Hz/1 kHz, and 100 Hz/1 kHz pulse repetition frequencies, respectively). These findings promote the study of using LIPUS to induce osteogenic differentiation and further encourage the exploration of LIPUS parameter optimization. The custom LIPUS system was successfully designed to allow extreme parameter variation, specifically PRF, and encourages further studies.}, number={9}, journal={IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL}, author={Marvel, Skylar and Okrasinski, Stan and Bernacki, Susan H. and Loboa, Elizabeth and Dayton, Paul A.}, year={2010}, month={Sep}, pages={1977–1984} }
 @article{hanson_marvel_bernacki_banes_aalst_loboa_2009, title={Osteogenic Effects of Rest Inserted and Continuous Cyclic Tensile Strain on hASC Lines with Disparate Osteodifferentiation Capabilities}, volume={37}, ISSN={["1573-9686"]}, DOI={10.1007/s10439-009-9648-7}, abstractNote={We investigated the effects of two types of cyclic tensile strain, continuous and rest inserted, on osteogenic differentiation of human adipose-derived adult stem cells (hASCs). The influence of these mechanical strains was tested on two hASC lines having different mineral deposition potential, with one cell line depositing approximately nine times as much calcium as the other hASC line after 14 days of culture in osteogenic medium on tissue culture plastic. Results showed that both continuous (10% strain, 1 Hz) and rest inserted cyclic tensile strain (10% strain, 1 Hz, 10 s rest after each cycle) regimens increased the amount and rate of calcium deposition for both high and low calcium depositing hASC lines as compared to unstrained controls. The response was similar for both types of tensile strain for a given cell line, however, cyclic tensile strain had a much stronger osteogenic effect on the high calcium depositing hASC line, suggesting that mechanical loading has a greater effect on cell lines that already have an innate ability to produce bone as compared to cell lines that do not. This is the first study to investigate the osteodifferentiation effects of cyclic tensile strain on hASCs and the first to show that both continuous (10%, 1 Hz) and rest inserted (10%, 1 Hz, 10 s rest) cyclic tensile strain accelerate hASC osteodifferentiation and increase calcium accretion.}, number={5}, journal={ANNALS OF BIOMEDICAL ENGINEERING}, author={Hanson, Ariel D. and Marvel, Skylar W. and Bernacki, Susan H. and Banes, Albert J. and Aalst, John and Loboa, Elizabeth G.}, year={2009}, month={May}, pages={955–965} }
 @article{van aalst_reed_han_andrady_hromadka_bernacki_kolappa_collins_loboa_2008, title={Cellular Incorporation Into Electrospun Nanofibers}, volume={60}, ISSN={0148-7043}, url={http://dx.doi.org/10.1097/sap.0b013e318168db3e}, DOI={10.1097/SAP.0b013e318168db3e}, abstractNote={Nanofibers are an emerging scaffold for tissue engineering. To date no one has reported cell incorporation into nanofibers. Human foreskin fibroblasts and human adipose-derived adult stem cells (hADAS) were grown to confluence, resuspended in phosphate-buffered saline, and then solubilized in polyvinyl alcohol (PVA). Nanofibers were created using an electrospinning technique across an electric potential of 20 kV. Cell interaction with nanofibers was assessed with optical microscopic imaging and scanning electron microscopy. PVA nanofibers with incorporated cells were then solubilized in phosphate-buffered saline; cell viability was assessed by trypan blue exclusion. Viable cells were allowed to proliferate. Chondrogenesis in fibroblasts was induced with TGF-&bgr;1. Both fibroblasts and hADAS survived the electrospinning process and were incorporated into PVA nanofibers. hADAS cell proliferation was negligible; however, fibroblasts proliferated and showed retained ability to undergo chondrogenesis. Cells can be incorporated into nanofibers, with maintained viability, proliferation, and function.}, number={5}, journal={Annals of Plastic Surgery}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={van Aalst, John A. and Reed, Courtney R. and Han, Li and Andrady, Tony and Hromadka, Michael and Bernacki, Susan and Kolappa, Kamalkumar and Collins, James B. and Loboa, Elizabeth G.}, year={2008}, month={May}, pages={577–583} }
 @misc{bernacki_wall_loboa_2008, title={Isolation of human mesenchymal stem cells from bone and adipose tissue}, volume={86}, journal={Stem cell culture}, author={Bernacki, S. H. and Wall, M. E. and Loboa, E. G.}, year={2008}, pages={257–278} }
 @article{mccullen_stevens_roberts_clarke_bernacki_gorga_loboa_2007, title={Characterization of electrospun nanocomposite scaffolds and biocompatibility with adipose-derived human mesenchymal stem cells}, volume={2}, number={2}, journal={International Journal of Nanomedicine}, author={McCullen, S. D. and Stevens, D. R. and Roberts, W. A. and Clarke, L. I. and Bernacki, S. H. and Gorga, R. E. and Loboa, E. G.}, year={2007}, pages={253–263} }
 @article{finger_sargent_dulaney_bernacki_loboa_2007, title={Differential effects on messenger ribonucleic acid expression by bone marrow-derived human mesenchymal stem cells seeded in agarose constructs due to ramped and steady applications of cyclic hydrostatic pressure}, volume={13}, ISSN={["1076-3279"]}, DOI={10.1089/ten.2006.0290}, abstractNote={This study investigated the differential effects of ramped and steady applications of cyclic hydrostatic pressure (CHP) on chondrogenic differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) in 3-dimensional culture in the absence of transforming growth factor-beta (TGF-beta). A custom hydrostatic pressure system was designed and manufactured. hMSCs were seeded in agarose and exposed to steady (7.5 MPa) or ramped (1 MPa to 7.5 MPa over a 14-day period) CHP for 4 h/d at f = 1 Hz for 14 days. Real-time reverse transcriptase polymerase chain reaction analysis was performed on days 0, 4, 9, and 14 to determine changes in messenger ribonucleic acid (mRNA) expression levels of Sox9, aggrecan, collagen I, and collagen II. Collagen II and aggrecan mRNA expression remained unchanged. Collagen I increased at day 4 in CHP specimens before decreasing to levels at or below same-day unloaded controls at days 9 and 14. On average, ramped and steady regimens of CHP increased Sox9, with the largest upregulation occurring at day 4 in response to steady pressure. These findings indicate that hydrostatic pressure may induce chondrogenesis in hMSC-seeded agarose constructs without TGF-beta, and that hMSCs are capable of withstanding high initial pressures that may initiate chondrogenesis faster than lower pressures.}, number={6}, journal={TISSUE ENGINEERING}, author={Finger, Allison R. and Sargent, Carolyn Y. and Dulaney, Katherine O. and Bernacki, Susan H. and Loboa, Elizabeth G.}, year={2007}, month={Jun}, pages={1151–1158} }
 @article{wall_bernacki_loboa_2007, title={Effects of serial passaging on the adipogenic and osteogenic differentiation potential of adipose-derived human mesenchymal stem cells}, volume={13}, ISSN={["1076-3279"]}, DOI={10.1089/ten.2006.0275}, abstractNote={Adipose-derived human mesenchymal stem cells (hMSCs) will be more valuable for tissue engineering applications if they can be extensively subcultured without loss of phenotype and multilineage differentiation ability. This study examined the effects of serial passaging on growth rate, gene expression, and differentiation potential of adipose-derived hMSCs. Differentiation was assessed by analyzing changes in messenger RNA (mRNA) expression of osteogenic and adipogenic marker genes and by determining production of calcium deposits and lipid vacuoles. Cells cultured in osteogenic medium for 2 weeks upregulated expression of alkaline phosphatase mRNA relative to cells in growth medium, and deposited calcium. Calcium deposition decreased in cells from passages 4 to 6 but returned to levels near or above those of primary cells by passage 10. Cells cultured in adipogenic medium upregulated expression of lipoprotein lipase and peroxisome proliferator activated receptor-gamma mRNA relative to cells in growth medium, and formed lipid vacuoles at all passages. By passage 8, however, cells in adipogenic medium also deposited calcium. Growth rate was stable through passage 5, then decreased. The results of this study indicate that adipose-derived hMSCs are capable of both adipogenic and osteogenic differentiation through 10 passages (34 population doublings) but that osteogenic differentiation may start to dominate at later passages.}, number={6}, journal={TISSUE ENGINEERING}, author={Wall, Michelle E. and Bernacki, Susan H. and Loboa, Elizabeth G.}, year={2007}, month={Jun}, pages={1291–1298} }
 @article{sumanasinghe_bernacki_loboa_2006, title={Osteogenic differentiation of human mesenchymal stem cells in collagen matrices: Effect of uniaxial cyclic tensile strain on bone morphogenetic protein (BMP-2) mRNA expression}, volume={12}, ISSN={["1076-3279"]}, DOI={10.1089/ten.2006.12.3459}, abstractNote={Human mesenchymal stem cells (hMSCs) differentiate down an osteogenic pathway with appropriate mechanical and/or chemical stimuli. This study describes the successful culture of hMSCs in 3D collagen matrices under mechanical strain. Bone marrow-derived hMSCs were seeded in linear 3D type I collagen matrices and subjected to 0%, 10%, or 12% uniaxial cyclic tensile strain at 1 Hz for 4 h/day for 7 or 14 days. Cell viability studies indicated that hMSCs remained viable throughout the culture period irrespective of the applied strain level. Real-time RT-PCR studies indicated a significant increase in BMP-2 mRNA expression levels in hMSCs strained at 10% compared to the same day unstrained controls after both 7 and 14 days. An increase in BMP-2 was also observed in hMSCs subjected to 12% strain, but the increase was significant only in the 14-day sample. This is the first report of the culture of bone marrow-derived hMSCs in 3D collagen matrices under cyclic strain, and the first demonstration that strain alone can induce osteogenic differentiation without the addition of osteogenic supplements. Induction of bone differentiation in 3D culture is a critical step in the creation of bioengineered bone constructs.}, number={12}, journal={TISSUE ENGINEERING}, author={Sumanasinghe, Ruwan D. and Bernacki, Susan H. and Loboa, Elizabeth G.}, year={2006}, month={Dec}, pages={3459–3465} }