Shu Jiang

author keywords: Vibrational Stark effect; Hemoglobin; 4-mercaptobenzonitrile; Enzyme kinetics; Electric field

MeSH headings : Animals; Catalysis; Kinetics; Mutation; Peroxidase / chemistry; Peroxidase / genetics; Peroxidases / chemistry; Peroxidases / genetics; Polychaeta / enzymology; Protein Conformation; Spectroscopy, Fourier Transform Infrared; Static Electricity; Vibration

topics (OpenAlex): Hemoglobin structure and function; Spectroscopy and Quantum Chemical Studies; Electron Spin Resonance Studies

TL;DR: By labeling the protein with 4-mercaptobenzonitrile (MBN), a Stark probe molecule, this work provides further evidence that the diffusion control of the catalytic process arises from the electrostatic repulsion between the enzyme and the negatively charged substrate. (via Semantic Scholar)

author keywords: Enzyme kinetics; Catalytic efficiency; Hemichrome; Resonance Raman; Molecular dynamics

MeSH headings : Animals; Biocatalysis; Heme / chemistry; Hemoglobins / chemistry; Hemoglobins / genetics; Histidine / chemistry; Histidine / genetics; Iron / chemistry; Kinetics; Molecular Dynamics Simulation; Mutagenesis, Site-Directed; Peroxidases / chemistry; Peroxidases / genetics; Polychaeta / chemistry; Polychaeta / enzymology; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; Thermodynamics; Threonine / chemistry; Threonine / genetics

topics (OpenAlex): Hemoglobin structure and function; Heme Oxygenase-1 and Carbon Monoxide; Erythrocyte Function and Pathophysiology

TL;DR: It is confirmed that subtle changes in the conformation of H55 affect the catalytic efficiency of DHP, and a balance of enzymatic rate and protein stability with respect to hemichrome formation appears to be optimum in wild type DHP (WT-DHP). (via Semantic Scholar)