@misc{kothari_singh_nath_kumar_rai_kaushal_omar_pandey_jain_2020, title={Immune Dysfunction and Multiple Treatment Modalities for the SARS-CoV-2 Pandemic: Races of Uncontrolled Running Sweat?}, volume={9}, ISSN={["2079-7737"]}, DOI={10.3390/biology9090243}, abstractNote={Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a global pandemic threat with more than 11.8 million confirmed cases and more than 0.5 million deaths as of 3 July 2020. Given the lack of definitive pharmaceutical interventions against SARS-CoV-2, multiple therapeutic strategies and personal protective applications are being used to reduce the risk of high mortality and community spread of this infection. Currently, more than a hundred vaccines and/or alternative therapeutic regimens are in clinical trials, and some of them have shown promising results in improving the immune cell environment and controlling the infection. In this review, we discussed high-performance multi-directory strategies describing the uncontrolled deregulation of the host immune landscape associated with coronavirus disease (COVID-19) and treatment strategies using an anti-neoplastic regimen. We also followed selected current treatment plans and the most important on-going clinical trials and their respective outcomes for blocking SARS-CoV-2 pathogenesis through regenerative medicine, such as stem cell therapy, chimeric antigen receptors, natural killer (NK) cells, extracellular vesicular-based therapy, and others including immunomodulatory regimens, anti-neoplastic therapy, and current clinical vaccine therapy.}, number={9}, journal={BIOLOGY-BASEL}, author={Kothari, Ashish and Singh, Vanya and Nath, Uttam Kumar and Kumar, Sandeep and Rai, Vineeta and Kaushal, Karanvir and Omar, Balram Ji and Pandey, Atul and Jain, Neeraj}, year={2020}, month={Sep} }
 @article{kumar_thompson_2009, title={Simultaneous excision of two transgene flanking sequences and resolution of complex integration loci}, volume={69}, ISSN={["1573-5028"]}, DOI={10.1007/s11103-008-9402-9}, abstractNote={In planta excision techniques have proven useful both for basic biology and applied biotechnology. In this report, we describe a simple site-specific recombination (SSR) strategy that simultaneously removes pre-defined DNA sequences from both sides of a transgenic "gene of interest," leaving only the desired gene and short sequences from the recombinase recognition site. We have used the FLP/FRT SSR system to provide a proof of concept, though any of several other SSR systems could be used in the same way. The frequency of double excision ranged from 33% to 83% in different transgenic lines. We show that a single SSR reaction can simultaneously carry out double excisions and resolve complex transgene loci at high frequency. The method has direct biotechnological application and provides a useful tool for basic research.}, number={1-2}, journal={PLANT MOLECULAR BIOLOGY}, author={Kumar, Sandeep and Thompson, William F.}, year={2009}, month={Jan}, pages={23–32} }
 @article{allen_flores-vergara_krasnyanski_kumar_thompson_2006, title={A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide}, volume={1}, ISSN={["1750-2799"]}, DOI={10.1038/nprot.2006.384}, abstractNote={We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from plant tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5-30 microg of total DNA from 200 mg of tissue fresh weight, depending on plant species and tissue source. It can be completed in as little as 5-6 h.}, number={5}, journal={NATURE PROTOCOLS}, author={Allen, G. C. and Flores-Vergara, M. A. and Krasnyanski, S. and Kumar, S. and Thompson, W. F.}, year={2006}, pages={2320–2325} }
 @misc{kumar_allen_thompson_2006, title={Gene targeting in plants: fingers on the move}, volume={11}, ISSN={["1360-1385"]}, DOI={10.1016/j.tplants.2006.02.002}, abstractNote={Zinc-finger endonucleases (ZFNs) make targeted double-stranded breaks in genomic DNA and, thus, stimulate recombination and repair processes at specific sites. ZFNs can now be harnessed to stimulate homologous recombination and gene targeting in plants, which represents a major step towards modifying the plant genome more precisely. ZFN-mediated gene targeting is likely to become a powerful tool for genome research and genetic engineering. Zinc-finger endonucleases (ZFNs) make targeted double-stranded breaks in genomic DNA and, thus, stimulate recombination and repair processes at specific sites. ZFNs can now be harnessed to stimulate homologous recombination and gene targeting in plants, which represents a major step towards modifying the plant genome more precisely. ZFN-mediated gene targeting is likely to become a powerful tool for genome research and genetic engineering.}, number={4}, journal={TRENDS IN PLANT SCIENCE}, author={Kumar, S and Allen, GC and Thompson, WF}, year={2006}, month={Apr}, pages={159–161} }
 @article{kumar_fladung_2003, title={Forest tree transgenesis and functional genomics: From fast forward to reverse genetics}, volume={52}, number={06-May}, journal={Silvae Genetica}, author={Kumar, S. and Fladung, M.}, year={2003}, pages={229-} }