@article{miranda_murphy_marshall_cowger_leath_2007, title={Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.)}, volume={114}, ISSN={0040-5752 1432-2242}, url={http://dx.doi.org/10.1007/s00122-007-0530-4}, DOI={10.1007/s00122-007-0530-4}, abstractNote={A single gene controlling powdery mildew resistance was identified in the North Carolina germplasm line NC96BGTD3 (NCD3) using genetic analysis of F(2) derived lines from a NCD3 X Saluda cross. Microsatellite markers linked to this Pm gene were identified and their most likely order was Xcfd7, 10.3 cM, Xgdm43, 8.6 cM, Xcfd26, 11.9 cM, Pm gene. These markers and the Pm gene were assigned to chromosome 5DL by means of Chinese Spring Nullitetrasomic (Nulli5D-tetra5A) and ditelosomic (Dt5DL) lines. A detached leaf test showed a distinctive disease reaction to six pathogen isolates among the NCD3 Pm gene, Pm2 (5DS) and Pm34 (5DL). An allelism test showed independence between Pm34 and the NCD3 Pm gene. Together, the tests provided strong evidence for the presence of a novel Pm gene in NCD3, and this gene was designated Pm35.}, number={8}, journal={Theoretical and Applied Genetics}, publisher={Springer Science and Business Media LLC}, author={Miranda, L. M. and Murphy, J. P. and Marshall, D. and Cowger, C. and Leath, S.}, year={2007}, month={Mar}, pages={1451–1456} } @article{miranda_perugini_srnic_brown-guedira_marshall_leath_murphy_2007, title={Genetic mapping of a Triticum monococcum-derived powdery mildew resistance gene in common wheat}, volume={47}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2007.01.0053}, abstractNote={Powdery mildew of wheat (Triticum aestivum L.) is a major fungal disease caused by Blumeria graminis DC f. sp. tritici A microsatellite linkage map was developed for the T. monococcum‐derived powdery mildew resistant gene present in the North Carolina germplasm line NCBGT96A6 (NCA6). Genetic analysis of F2‐derived lines from the cross NCA6 × ‘Saluda’ indicated a single gene controlled powdery mildew resistance. Four microsatellite markers linked to the NCA6 Pm gene mapped to chromosome 7AL. The most likely order was Xcfa2123‐0.9 cM–Xbarc121‐1.7 cM resistance gene/Xcfa2019‐3.0 cM‐Xgwm332 A detached‐leaf test indicated the disease reaction response of the NCA6 Pm gene was different from the five known alleles at the Pm1 locus on 7AL. Deletion interval mapping showed a large physical to genetic distance ratio for these microsatellite marker loci. This may be due to suppressed recombination between the introgressed T. monococcum segment and the homologous region of the T. aestivum cultivar Saluda. Our results suggested that the NCA6 Pm gene is likely a novel source of resistance to powdery mildew but additional allelism studies are needed to establish the relationship between this locus and the other known Pm loci on 7AL.}, number={6}, journal={CROP SCIENCE}, author={Miranda, L. M. and Perugini, L. and Srnic, G. and Brown-Guedira, G. and Marshall, D. and Leath, S. and Murphy, J. P.}, year={2007}, pages={2323–2329} } @article{miranda_murphy_marshall_leath_2006, title={Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.)}, volume={113}, ISSN={["1432-2242"]}, DOI={10.1007/s00122-006-0397-9}, abstractNote={Powdery mildew is a major fungal disease in wheat growing areas worldwide. A novel source of resistance to wheat powdery mildew present in the germplasm line NC97BGTD7 was genetically characterized as a monogenic trait in greenhouse and field trials using F(2) derived lines from a NC97BGTD7 X Saluda cross. Microsatellite markers were used to map and tag this resistance gene, now designated Pm34. Three co-dominant microsatellite markers linked to Pm34 were identified and their most likely order was established as: Xbarc177-5D, 5.4cM, Pm34, 2.6cM, Xbarc144-5D, 14cM, Xgwm272-5D. These microsatellite markers were previously mapped to the long arm of the 5D chromosome and their positions were confirmed using Chinese Spring nullitetrasomic Nulli5D-tetra5A and ditelosomic Dt5DL lines. Pm2, the only other known Pm gene on chromosome 5D, has been mapped to the short arm and its specificity is different from that of Pm34.}, number={8}, journal={THEORETICAL AND APPLIED GENETICS}, author={Miranda, L. M. and Murphy, J. P. and Marshall, D. and Leath, S.}, year={2006}, month={Nov}, pages={1497–1504} } @article{srnic_murphy_lyerly_leath_marshall_2005, title={Inheritance and chromosomal assignment of powdery mildew resistance genes in two winter wheat germplasm lines}, volume={45}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2004.0530}, abstractNote={Powdery mildew of wheat (Triticum aestivum L.), caused by Blumeria graminis DC f. sp. tritici Em. Marchal, occurs annually in eastern North America resulting in reduced grain yield and end‐use quality in susceptible cultivars. The objectives of this study were to determine the inheritance, chromosomal location, and linkage with molecular markers of powdery mildew resistance genes in the two recently released germplasm lines NC96BGTA4 and NC99BGTAG11. Between 99 and 194 F2:3 progenies plus parents in two populations, ‘Saluda’ × NC96BGTA4 and Saluda × NC99BGTAG11, were evaluated in greenhouse and field nurseries for reaction to powdery mildew infection. Results indicated that the germplasm lines each contained a different, partially dominant, major resistance gene. The two segregating populations were subjected to amplified fragment length polymorphism (AFLP) and simple sequence repeat, or microsatellite (SSR) analyses. Both resistance genes were located on the long arm of chromosome 7A. The most likely locus order indicated that the resistance gene in NC96BGTA4 was flanked by the SSR loci Xbarc292 and Xwmc525 The resistance gene in NC99BGTAG11 was most likely flanked by the AFLP markers XE38M54‐196 and XE36M55‐126, and the SSR loci Xgwm332 and Xwmc525 Both genes mapped to a chromosome arm that contains the powdery mildew resistance loci Pm1 and Pm9 The resistance genes in the two germplasms are different from the Pm1a allele. Our mapping results suggested that the resistance genes were not alleles at the Pm1 or Pm9 loci, but further allelism tests are necessary to determine the relationships both between the two genes themselves and between the two genes and named Pm loci on chromosome 7AL.}, number={4}, journal={CROP SCIENCE}, author={Srnic, G and Murphy, JP and Lyerly, JH and Leath, S and Marshall, DS}, year={2005}, pages={1578–1586} } @article{murphy_navarro_leath_bowman_weisz_ambrose_pate_fountain_2004, title={Registration of 'NC-Neuse' wheat}, volume={44}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2004.1479}, abstractNote={Crop ScienceVolume 44, Issue 4 p. 1479-1480 Registration of Cultivar Registration of ‘NC-Neuse’ Wheat J.P. Murphy, Corresponding Author J.P. Murphy njpm@unity.ncsu.edu Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (njpm@unity.ncsu.edu)Search for more papers by this authorR.A. Navarro, R.A. Navarro Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorS. Leath, S. Leath Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorD.T. Bowman, D.T. Bowman Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorP.R. Weisz, P.R. Weisz Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorL.G. Ambrose, L.G. Ambrose Beaufort Co. CES, 155 Airport Rd., Washington, NC, 27889Search for more papers by this authorM.H. Pate, M.H. Pate MidState Mills, Inc., P.O. Box 350, Newton, NC, 28658Search for more papers by this authorM.O. Fountain, M.O. Fountain Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author J.P. Murphy, Corresponding Author J.P. Murphy njpm@unity.ncsu.edu Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (njpm@unity.ncsu.edu)Search for more papers by this authorR.A. Navarro, R.A. Navarro Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorS. Leath, S. Leath Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorD.T. Bowman, D.T. Bowman Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorP.R. Weisz, P.R. Weisz Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorL.G. Ambrose, L.G. Ambrose Beaufort Co. CES, 155 Airport Rd., Washington, NC, 27889Search for more papers by this authorM.H. Pate, M.H. Pate MidState Mills, Inc., P.O. Box 350, Newton, NC, 28658Search for more papers by this authorM.O. Fountain, M.O. Fountain Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author First published: 01 July 2004 https://doi.org/10.2135/cropsci2004.1479Citations: 17 Research supported in part by grants from the North Carolina Small Grains Growers Association, the North Carolina Foundation Seed Producers, Inc., and the North Carolina Crop Improvement Association. Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume44, Issue4July–August 2004Pages 1479-1480 RelatedInformation}, number={4}, journal={CROP SCIENCE}, author={Murphy, JP and Navarro, RA and Leath, S and Bowman, DT and Weisz, PR and Ambrose, LG and Pate, MH and Fountain, MO}, year={2004}, pages={1479–1480} } @article{fraser_murphy_leath_van sanford_2003, title={Effect of inoculation with selected isolates of Stagonospora nodorum on field evaluations of host resistance in winter wheat}, volume={87}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS.2003.87.10.1213}, abstractNote={ Although Stagonospora nodorum blotch occurs annually in North Carolina, selection for resistance in wheat (Triticum aestivum) breeding nurseries is hampered by the infrequent occurrence of heavy and timely disease pressure. The objective of this study was to compare estimates of host resistance in a population of 147 random winter wheat lines evaluated in epidemics produced by natural infection versus epidemics supplemented by inoculation with selected isolates. Two isolates were chosen from a set of 43 collected in North Carolina based on their aggressiveness on four wheat cultivars in a controlled environment test. Field experiments utilized a split-plot design with three replications. The main plots were inoculation treatments and the subplots were the 147 wheat genotypes. The inoculation treatments were (i) selected isolate A (more aggressive) alone, (ii) selected isolate B (less aggressive) alone, (iii) a combination of isolates A plus B, and (iv) natural infection. Selected isolate treatments were applied at Feekes growth stage 9 to 10.1, and disease intensity was measured two or three times at 14-day intervals postinoculation. The study was conducted at one location in the 1996-97 season and two locations in the 1997-98 season. High levels of natural infection occurred, and no differences were observed among the four inoculation treatments for mean disease intensity in any of the three environments. Within environments, genotype-by-inoculation treatment variance was significant in the two environments inoculated with selected isolates at growth stage 9 but not in the environment inoculated at growth stage 10.1. Magnitudes of genetic variation and heritability for Stagonospora nodorum blotch resistance were not consistently associated with main plot treatments, and inoculation with selected isolates masked genetic variation for resistance in two treatments in one environment. Genotype rank correlations for Stagonospora nodorum blotch resistance between inoculation treatments varied from zero to 0.69 within environments, but only a single correlation between inoculation treatments in different environments was observed. Estimates of host resistance in epidemics supplemented with selected isolates did not consistently agree with estimates in epidemics produced by natural infection. Our results did not support the routine use of supplemental inoculation of wheat breeding nurseries with selected isolates of S. nodorum as a means of increasing genetic gain for host resistance. }, number={10}, journal={PLANT DISEASE}, author={Fraser, DE and Murphy, JP and Leath, S and Van Sanford, DA}, year={2003}, month={Oct}, pages={1213–1220} } @article{murphy_navarro_leath_bowman_2002, title={Registration of 'NC Hulless' oat}, volume={42}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2002.0311}, abstractNote={Crop ScienceVolume 42, Issue 1 p. 311-311 Registration of Cultivars Registration of ‘NC Hulless’ Oat J.P. Murphy, Corresponding Author J.P. Murphy njpm@unity.ncsu.edu Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (njpm@unity.ncsu.edu)Search for more papers by this authorR.A. Navarro, R.A. Navarro Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorS. Leath, S. Leath USDA-ARS, Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorD.T. Bowman, D.T. Bowman Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author J.P. Murphy, Corresponding Author J.P. Murphy njpm@unity.ncsu.edu Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (njpm@unity.ncsu.edu)Search for more papers by this authorR.A. Navarro, R.A. Navarro Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorS. Leath, S. Leath USDA-ARS, Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorD.T. Bowman, D.T. Bowman Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author First published: 01 January 2002 https://doi.org/10.2135/cropsci2002.3110 Research supported in part by grants from the North Carolina Small Grains Growers Association, Inc. and the USDA-ARS. Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Volume42, Issue1January–February 2002Pages 311-311 RelatedInformation}, number={1}, journal={CROP SCIENCE}, author={Murphy, JP and Navarro, RA and Leath, S and Bowman, DT}, year={2002}, pages={311–311} } @article{murphy_navarro_leath_2002, title={Registration of NC99BGTAG11 wheat germplasm resistant to powdery mildew}, volume={42}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2002.1382}, abstractNote={Crop ScienceVolume 42, Issue 4 p. 1382-1382 Registration of Germplasm Registration of NC99BGTAG11 Wheat Germplasm Resistant to Powdery Mildew J.P. Murphy, Corresponding Author J.P. Murphy njpm@unity.ncsu.edu Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (njpm@unity.ncsu.edu)Search for more papers by this authorR.A. Navarro, R.A. Navarro Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorS. Leath, S. Leath USDA-ARS, Dep. Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author J.P. Murphy, Corresponding Author J.P. Murphy njpm@unity.ncsu.edu Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (njpm@unity.ncsu.edu)Search for more papers by this authorR.A. Navarro, R.A. Navarro Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorS. Leath, S. Leath USDA-ARS, Dep. Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author First published: 01 July 2002 https://doi.org/10.2135/cropsci2002.1382Citations: 7 Research supported in part by the North Carolina Small Grains Growers Association, Inc. Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume42, Issue4July–August 2002Pages 1382-1382 RelatedInformation}, number={4}, journal={CROP SCIENCE}, author={Murphy, JP and Navarro, RA and Leath, S}, year={2002}, pages={1382–1382} } @article{everts_leath_finney_2001, title={Impact of powdery mildew and leaf rust on milling and baking quality of soft red winter wheat}, volume={85}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS.2001.85.4.423}, abstractNote={ Changes in milling and baking quality (especially flour yield) of soft red winter wheat can have a large economic impact on flour mills. To determine the relationship between early-season powdery mildew and late-season leaf rust on flour yield, flour protein, alkaline water retention capacity, and kernel texture (softness equivalent), a study was conducted over 2 years at Kinston and Plymouth, NC. Different levels of powdery mildew and leaf rust developed on three winter wheat cultivars that varied in levels of disease resistance, the presence of seed treatment, and the presence and timing of foliar fungicide application. In Kinston and Plymouth in 1989-90, where leaf rust occurred early, the softness equivalent score was lower in wheat grown from seed treated with triadimenol. The following year, when the leaf rust epidemic increased later, foliar fungicide application reduced disease and resulted in lower softness equivalent scores in both Plymouth and Kinston for cv. Saluda and in Kinston for cv. Coker 983. A regression model was developed to describe the relationship between the log of the area under the disease progress curves and adjusted flour yield (AFY). The AFY of Saluda was reduced in the presence of powdery mildew such that %AFY = 103.96 - 0.92 (log AUMPC). }, number={4}, journal={PLANT DISEASE}, author={Everts, KL and Leath, S and Finney, PL}, year={2001}, month={Apr}, pages={423–429} } @article{walker_leath_hagler_murphy_2001, title={Variation among isolates of Fusarium graminearum associated with Fusarium head blight in North Carolina}, volume={85}, ISSN={["0191-2917"]}, DOI={10.1094/PDIS.2001.85.4.404}, abstractNote={ Fusarium head blight (FHB) can reduce yield of wheat and decrease the value of harvested grain by accumulation of detrimental toxins. Understanding the variability of the fungal population associated with infection could improve disease control strategies. Sixty-six isolates of Fusarium graminearum associated with FHB were collected in North Carolina and tested for in vitro growth rate, in vitro production of deoxynivalenol (DON) and zearalenone, and pathogenicity on three cultivars of soft red winter wheat. Significant differences among isolates were found for all three traits. Randomly Amplified Polymorphic DNA (RAPD) analysis revealed high levels of genotypic diversity among isolates. Isolates of F. graminearum, F. culmorum, and F. avenaceum acquired from the Pennsylvania State University Fusarium Center were included for comparison in all tests. In vivo levels of DON were measured for the five isolates associated with the highest levels of disease and the five isolates associated with the lowest levels of disease, and no significant differences were found. However, all ten isolates produced detectable levels of DON in vivo. Mean disease ratings ranged from 3.4 to 96.4%, in vitro (DON) levels ranged from 0 to 7176.2 ppm, and zearalenone ranged from 0 to 354.7 ppm, among isolates. A multiple regression model using in vitro growth, in vitro DON, and zearalenone production, collection location, wheat cultivar of isolate origin, plot, tillage conditions, and previous crop as independent variables and percent blighted tissue as the dependent variable was developed. The cumulative R2 value for the model equaled 0.27 with in vitro rate of growth making the largest contribution. Analysis of phenotype and genotype among isolates demonstrated diversity in a single plot, in a single location, and in North Carolina. Genotypic and phenotypic diversity were significant under both conventional and reduced tillage conditions, and diversity was high regardless of whether the previous crop had been a host or non-host for F. graminearum. These data indicate a variable pathogen population of F. graminearum exists in North Carolina, and members of this population can be both highly pathogenic on wheat and produce high levels of detrimental toxins, indicating a potential threat for problems with FHB within the state. }, number={4}, journal={PLANT DISEASE}, author={Walker, SL and Leath, S and Hagler, WM and Murphy, JP}, year={2001}, month={Apr}, pages={404–410} } @article{navarro_murphy_leath_shi_2000, title={Registration of NC97BGTAB9 and NC97BGTAB10 wheat germplasm lines resistant to powdery mildew}, volume={40}, number={5}, journal={Crop Science}, author={Navarro, R. A. and Murphy, J. P. and Leath, S. and Shi, A.}, year={2000}, pages={1508–1509} } @article{brown-guedira_cox_gill_sears_leath_1999, title={Registration of KS96WGRC37 powdery mildew-resistant hard white winter wheat germplasm}, volume={39}, number={2}, journal={Crop Science}, author={Brown-Guedira, G. L. and Cox, T. S. and Gill, B. S. and Sears, R. G. and Leath, S.}, year={1999}, pages={596} } @article{cox_bockus_gill_sears_harvey_leath_brown-guedira_1999, title={Registration of KS96WGRC40 hard red winter wheat germplasm resistant to wheat curl mite, stagnospora leaf blotch, and septoria leaf blotch}, volume={39}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1999.0011183X003900020070x}, abstractNote={KS96WGRC40 (Reg. no. GP-559, PI 604225) is a hard red winter wheat (Triticum aestivum L.) with resistance to wheat curl mite, stagonospora leaf blotch and septoria leaf blotch developed cooperatively by the USDA-ARS, the Kansas Agricultural Experiment Station, and the Wheat Genetics Resource Center. It was released as a germplasm in August 1996. The pedigree of KS96WGRC40 is KS93U69*2/TA 2397. KS93U69 is a sister line of KS90WGRC10 (TAM 107'*3/TA 2460), and TA 2397 and TA 2460 are accessions ofAegilops tauschii Coss. collected near Sisiar in Afghanistan and Khoshyailagh in Iran, respectively. KS96WGRC40 was formed by bulking seed from six plants of the germplasm line KS95WGRC33 that showed high levels of resistance to the wheat curl mite (Aceria tosichilla Keifer). Wheat curl mites of the Wakeeney strain were used to infest 11-d-old seedlings at the rate of five mites per plant. Mite populations per plant 10 d later were as follows: TAM 107, 71.2; KS93U69, 53.3; TA2397, 0.0; KS96WGRC40, 6.2. KS96WGRC40, like KS95WGRC33, is resistant to stagonospora and septoria leaf blotches [caused by Stagonospora nodorum (Berk.) Castellani & E.G. Germane and Septoria tritici Roberge in Desmaz., respectively] when inoculated as seedlings in the greenhouse and under natural infection in the field. Both germplasms had significantly longer green leaf duration than TAM 107 or KS93U69 in the field under septoria leaf-blotch infection at Parsons, KS, in 1993 and 1994, and under stagonospora leaf-blotch infection at Hutchinson and Manhattan, KS, and Laurel Springs, NC, in 1996. They had lower seedling-infection scores for both diseases than did TAM 107 in greenhouse tests at Manhattan. When inoculated in seedling experiments with a field-collected isolate of Se. tritici from Riley County, Kansas, the germplasms had 4% of leaf area infected, compared with 28% for KS93U69, 41% for TAM 107, and 29% for 'Karl 92', the resistant check cultivar. In replicated tests in the greenhouse in 1995, seedlings of KS95WGRC33 inoculated with St. nodorum had 36% of leaf area infected, compared with 50.4% for TAM 107, and 23.5% for Jagger, the resistant check cultivar. The wheat curl mite and leaf blotch resistance of KS96WGRC40 are derived from TA 2397. The genetic basis of the resistance has not been determined. KS96WGRC40 also carries the gene Lr41 for resistance to leaf rust (caused by Puccinia recondita Roberge ex Desmaz.) from TA 2460 via KS93U69. It is similar to TAM 107 in days to heading, plant height, and general phenotype. Small quantities (2 g) of seed of KS96WGRC40 are available upon written request. Appropriate recognition of source should be given when this germplasm contributes to research or development of a new breeding line or cultivar. Seed stocks are maintained by the Wheat Genetics Resource Center, Dep. of Agronomy, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502.}, number={2}, journal={CROP SCIENCE}, author={Cox, TS and Bockus, WW and Gill, BS and Sears, RG and Harvey, TL and Leath, S and Brown-Guedira, GL}, year={1999}, pages={597–597} } @article{murphy_leath_huynh_navarro_shi_1999, title={Registration of NC96BGTA4, NC96BGTA5, and NC96BGTA6 wheat germplasm}, volume={39}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1999.0011183X003900030065x}, abstractNote={Crop ScienceVolume 39, Issue 3 cropsci1999.0011183X003900030065x p. 883-884 Registration of Germplasm Registration of NC96BGTA4, NC96BGTA5, and NC96BGTA6 Wheat Germplasm J. P. Murphy, Corresponding Author J. P. Murphy [email protected] Dep. of Crop ScienceCorresponding author ([email protected]).Search for more papers by this authorS. Leath, S. Leath Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorD. Huynh, D. Huynh Dep. of Crop ScienceSearch for more papers by this authorR. A. Navarro, R. A. Navarro Dep. of Crop ScienceSearch for more papers by this authorA. Shi, A. Shi USDA-ARS and Dep. of Plant PathologySearch for more papers by this author J. P. Murphy, Corresponding Author J. P. Murphy [email protected] Dep. of Crop ScienceCorresponding author ([email protected]).Search for more papers by this authorS. Leath, S. Leath Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorD. Huynh, D. Huynh Dep. of Crop ScienceSearch for more papers by this authorR. A. Navarro, R. A. Navarro Dep. of Crop ScienceSearch for more papers by this authorA. Shi, A. Shi USDA-ARS and Dep. of Plant PathologySearch for more papers by this author First published: 01 May 1999 https://doi.org/10.2135/cropsci1999.0011183X003900030065xCitations: 7AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article.Citing Literature Volume39, Issue3May–June 1999Pages 883-884 RelatedInformation}, number={3}, journal={CROP SCIENCE}, author={Murphy, JP and Leath, S and Huynh, D and Navarro, RA and Shi, A}, year={1999}, pages={883–884} } @article{murphy_leath_huynh_navarro_shi_1999, title={Registration of NC97BGTD7 and NC97BGTD8 wheat germplasms resistant to powdery mildew}, volume={39}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1999.0011183X003900030066x}, abstractNote={and Ma. Saluda contains the Pm3a gene (3) and was susceptible in all greenhouse and evaluations conducted in the development of the germplasms. The direct diploid to hexaploid transfer methodology followed during backcrossing was similar to that outlined by Gill and Raupp (1). We utilized A-genome diploids as donor parents in place of the D-genome diploids described in that study. The hexaploid x diploid cross, Fj embryo rescue, and development of BC,F,, BC^, and BC2F2 seed were conducted during the 1988 to 1991 winter greenhouse seasons. Field selection using the pedigree breeding method was initiated with BC2F2 bulk populations in the 1991-1992 season. Natural powdery mildew epiphytotics occurred each year. Selection was primarily for mildew resistance during Feekes (2) Growth Stages 8 to 10.5, but whenever possible additional selection for heading date, plant height, and straw strength was conducted using the Saluda phenotype as the benchmark. Each germplasm line traces to a single headrow harvested in 1996. Laboratory evaluations for powdery mildew resistance using the detached leaf technique were conducted during development of these germplasms and again just prior to release. Laboratory evaluations were completed with 2-cm pieces of the primary leaves floated on 0.5% water agar amended with 50 mg L~' benzimidazole and evaluated as summarized elsewhere (3). Each line was tested for homogeneity by inoculating two replicate leaf pieces from 16 plants with four distinct isolates. Line NC96BGTA6 had 3.8% susceptible offtypes, whereas NC96BGTA4 and NC96BGTA5 were homogeneous. In addition, the three germplasms were inoculated with 30 isolates of the powdery mildew fungus with distinct differences in virulence formula and aggressiveness. These isolates had virulence to all previously identified alleles for powdery mildew resistance, with the possible exception ofPmlS. NC96BGTA4, NC96BGTA5, and NC96BGTA6 showed susceptible reactions to the 6, 0, and 1 isolates, respectively. None exhibited a susceptible reaction to commonly occurring isolates. Data and pedigree analysis showed that these germplasms contained at least one resistance gene in addition to Pm3a. Small quantities of seed (2 g) of each germplasm line are available upon written request to the corresponding author. Appropriate recognition of source should be given if this germplasm contributes to research or development of new cultivars.}, number={3}, journal={CROP SCIENCE}, author={Murphy, JP and Leath, S and Huynh, D and Navarro, RA and Shi, A}, year={1999}, pages={884–885} } @article{shi_leath_murphy_1998, title={A major gene for powdery mildew resistance transferred to common wheat from wild einkorn wheat}, volume={88}, ISSN={["0031-949X"]}, DOI={10.1094/PHYTO.1998.88.2.144}, abstractNote={ A major gene for resistance to wheat powdery mildew (Blumeria graminis f. sp. tritici = Erysiphe graminis f. sp. tritici) has been successfully transferred into hexaploid common wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) from wild einkorn wheat (Triticum monococcum subsp. aegilopoides, 2n = 2x = 14, AA). NC96BGTA5 is a germ plasm line with the pedigree Saluda × 3/PI427662. The response patterns for powdery mildew resistance in NC96BGTA5 were tested with 30 differential isolates of B. graminis f. sp. tritici, and the line was resistant to all tested isolates. The analyses of P1, P2, F1, F2, and BC1F1 populations derived from NC96BGTA5 revealed two genes for wheat powdery mildew resistance in the NC96BGTA5 line. One gene, Pm3a, was from its recurrent parent Saluda, and the second was a new gene introgressed from wild einkorn wheat. The gene was determined to be different from Pm1 to Pm21 by gene-for-gene and pedigree analyses. The new gene was identified as linked to the Pm3a gene based on the F2 and BC1F1 populations derived from a cross between NC96BGTA5 and a susceptible cultivar NK-Coker 68-15, and the data indicated that the gene was located on chromosome 1A. It is proposed that this new gene be designated Pm25 for wheat powdery mildew resistance in NC96BGTA5. Three random amplified polymorphic DNA markers, OPX061050, OPAG04950, and OPAI14600, were found to be linked to this new gene. }, number={2}, journal={PHYTOPATHOLOGY}, author={Shi, AN and Leath, S and Murphy, JP}, year={1998}, month={Feb}, pages={144–147} } @article{walker_leath_murphy_lommel_1998, title={Selection for resistance and tolerance to oat mosaic virus and oat golden stripe virus in hexaploid oats}, volume={82}, ISSN={["0191-2917"]}, DOI={10.1094/PDIS.1998.82.4.423}, abstractNote={ Coker 716, a hexaploid oat cultivar resistant to both oat mosaic virus (OMV) and oat golden stripe virus (OGSV) was crossed to three susceptible cultivars (Brooks, Madison, and Tech) to form three individual populations. Individual breeding lines were derived from each cross in the F2 generation and tested in plots consisting of equally spaced individual hills in OMV- and OGSV-infested soils and non-infested soils to evaluate resistance and yield loss of individual lines. Foliar symptoms, harvest index, and yield loss were examined as selection criteria for resistant genotypes. The study was conducted over 2 years at two North Carolina locations that differed in soil type and climate. Multiple regression models describing yield loss in each cross due to rating, year, and location were calculated. Coefficients of multiple determination in these models ranged from 0.39 to 0.51. Yield loss ranged from 39 to 60% among different crosses. Infection by OMV and OGSV accounted for the majority of yield loss in two of the populations. Disease severity varied widely over years and locations. The results suggest that selection of lines with symptomatic tissue of 10% or less, or selection of tolerant lines, is needed for breeding progress. }, number={4}, journal={PLANT DISEASE}, author={Walker, SL and Leath, S and Murphy, JP and Lommel, SA}, year={1998}, month={Apr}, pages={423–427} } @article{niewoehner_leath_1998, title={Virulence of Blumeria graminis f. sp. tritici on winter wheat in the eastern United States}, volume={82}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS.1998.82.1.64}, abstractNote={ Samples of perithecia of Blumeria graminis f. sp. tritici from senescing wheat leaves were collected by cooperators from 17 states. Ascospores were discharged from perithecia and single-spore isolates were characterized for virulence genes using a differential host series containing 15 known resistance genes. A total of 520 isolates from 17 states were characterized in 1993 and 1994. Virulence frequencies and complexity and racial composition were examined. The data were analyzed for associations among sets of virulence genes and the geographical distribution of phenotypes. Virulence to Pm3c, Pm3f, pm5, Pm6, and Pm7 was present in all states surveyed. Since 1990, virulence to Pm3a has increased in the northeast, and virulence to Pm1, Pm4b, Pm8, and Pm17 has increased across the area surveyed. The resistance genes Pm12 and Pm16 remain highly effective in the southeastern United States. An increase in virulence frequencies and complexity of isolates was observed. }, number={1}, journal={PLANT DISEASE}, author={Niewoehner, AS and Leath, S}, year={1998}, month={Jan}, pages={64–68} } @article{murphy_griffey_finney_leath_1997, title={Agronomic and grain quality evaluations of Triticum aestivum x Aegilops tauschii backcross populations}, volume={37}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1997.0011183X003700060047x}, abstractNote={Aegilops tauschii Coss., a diploid progenitor of common wheat, Triticum aestivum L., is a valuable source of pest resistance alleles. However, interspecific populations generated for pest‐resistant germplasm development may contain beneficial alleles for other important traits. The objective of this research was to evaluate eight agronomic and grain quality traits in three soft red winter wheat × Ae. tauschii backcross populations. A total of 385 BC2F2‐derived lines were grown at locations in North Carolina and Virginia for two seasons. Grain quality evaluations were conducted at the USDA‐ARS Soft Wheat Quality Laboratory. Fifty‐four percent of lines did not differ significantly from their recurrent parent, averaged over all eight traits. In general, distributions were negatively skewed for grain yield and test weight and positively skewed for heading date, plant height, flour protein concentration, and alkaline water retention capacity. Line distributions for flour yield and softness equivalent were population dependent. Twenty‐three lines were significantly superior to their recurrent parent for one or more grain quality traits and similar to the recurrent parent for all remaining traits. Researchers who generate interspecific T. aestivum × Ae. tauschii populations for pest‐resistant germplasm development can identify lines with beneficial alleles governing other traits in an acceptable cultivated background if the progeny undergo additional screening.}, number={6}, journal={CROP SCIENCE}, author={Murphy, JP and Griffey, CA and Finney, PL and Leath, S}, year={1997}, pages={1960–1965} } @article{murphy_navarro_leath_murphy_bowman_1997, title={Registration of 'Rodgers' oat}, volume={37}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1997.0011183X003700030073x}, abstractNote={Crop ScienceVolume 37, Issue 3 cropsci1997.0011183X003700030073x p. 1017-1017 Registration of Cultivars Registration of ‘Rodgers’ Oat J. P. Murphy, Corresponding Author J. P. Murphy [email protected] Dep. of Crop ScienceCorresponding author ([email protected]).Search for more papers by this authorR. A. Navarro, R. A. Navarro Dep. of Crop ScienceSearch for more papers by this authorS. Leath, S. Leath USDA-ARS and Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorC. F. Murphy, C. F. Murphy USDA-ARS, BARC-West, Beltsville, MD, 20705Search for more papers by this authorD. T. Bowman, D. T. Bowman Dep. of Crop ScienceSearch for more papers by this author J. P. Murphy, Corresponding Author J. P. Murphy [email protected] Dep. of Crop ScienceCorresponding author ([email protected]).Search for more papers by this authorR. A. Navarro, R. A. Navarro Dep. of Crop ScienceSearch for more papers by this authorS. Leath, S. Leath USDA-ARS and Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorC. F. Murphy, C. F. Murphy USDA-ARS, BARC-West, Beltsville, MD, 20705Search for more papers by this authorD. T. Bowman, D. T. Bowman Dep. of Crop ScienceSearch for more papers by this author First published: 01 May 1997 https://doi.org/10.2135/cropsci1997.0011183X003700030073xCitations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume37, Issue3May–June 1997Pages 1017-1017 RelatedInformation}, number={3}, journal={CROP SCIENCE}, author={Murphy, JP and Navarro, RA and Leath, S and Murphy, CF and Bowman, DT}, year={1997}, pages={1017–1017} } @article{brown-guedira_gill_cox_leath_1997, title={Transfer of disease resistance genes from Triticum araraticum to common wheat}, volume={116}, number={2}, journal={Plant Breeding}, author={Brown-Guedira, G. L. and Gill, B. S. and Cox, T. S. and Leath, S.}, year={1997}, pages={105–112} } @article{leath_cox_bockus_1994, title={Resistance to Septoria tritici and S. nodorum in common wheat x Triticum tauschii backcross populations}, volume={38}, number={3}, journal={Hodowla Roslin, Aklimatyzacja i Nasiennictwo}, author={Leath, S. and Cox, T. S. and Bockus, W. W.}, year={1994}, pages={165} } @article{leath_scharen_dietzholmes_lund_1993, title={FACTORS ASSOCIATED WITH GLOBAL OCCURRENCES OF SEPTORIA-NODORUM BLOTCH AND SEPTORIA-TRITICI BLOTCH OF WHEAT}, volume={77}, ISSN={["0191-2917"]}, DOI={10.1094/PD-77-1266}, abstractNote={Changes in incidence and severity of Septoria nodorum blotch (SNB) and Septoria tritici blotch (STB) have been noted in recent years in several wheat-growing areas of the world. A questionnaire was designed to identify factors associated with occurrence and development of these diseases. The questionnaire was sent to Septoria workers worldwide, and 71 responses were received and analyzed. The most important factors found relating to disease occurrence were: 1) latitude, 2) non-growing season precipitation, 3) growing season precipitation, 4) application of phosphorus, and 5) frequency of minimum or reduced tillage}, number={12}, journal={PLANT DISEASE}, author={LEATH, S and SCHAREN, AL and DIETZHOLMES, ME and LUND, RE}, year={1993}, month={Dec}, pages={1266–1270} } @article{leath_bruckner_1991, title={REACTION OF WINTER OAT GERM PLASM TO AN EPIDEMIC OF OAT POWDERY MILDEW}, volume={75}, ISSN={["0191-2917"]}, DOI={10.1094/PD-75-0807}, abstractNote={In the spring of 1989, an epidemic of oat powdery mildew occurred naturally in four randomized blocks near Tifton, Georgia, in which 31 winter oat lines had been planted in the fall of 1988, Plots were rated on 14 and 18 April for percentage of foliage covered with mildew and for infection type (0-4 scale). Differences existed among cultivars on both assessment dates. Severity levels increased from a mean of 29.2 to 32.9% between assessment dates, and all cultivars showed symptoms; oat lines ranged from 7.5 to 59,4% in severity and from 1.4 to 4.0 in infection type (...)}, number={8}, journal={PLANT DISEASE}, author={LEATH, S and BRUCKNER, PL}, year={1991}, month={Aug}, pages={807–809} } @article{leath_heun_1990, title={IDENTIFICATION OF POWDERY MILDEW RESISTANCE GENES IN CULTIVARS OF SOFT RED WINTER-WHEAT}, volume={74}, ISSN={["0191-2917"]}, DOI={10.1094/PD-74-0747}, abstractNote={Twenty-two soft red winter wheat (Triticum aestivum) cultivars were inoculated with isolates of Erysiphe graminis f. sp. tritici to determine genes for resistance. Cultivars were tested with a total of 27 isolates that had been characterized from reactions on differential host lines. Genes determinations were completed separately in two laboratories with different isolates and the results were combined. Intact 10-day-old seedlings or detached primary leaves on benzimidazole-amended agar were inoculated, and evaluations based on pustule number and type were made 10-14 days later (...)}, number={10}, journal={PLANT DISEASE}, author={LEATH, S and HEUN, M}, year={1990}, month={Oct}, pages={747–752} } @article{leath_thakur_leonard_1990, title={VARIATION IN EXPRESSION OF MONOGENIC RESISTANCE IN CORN TO EXSEROHILUM-TURCICUM RACE-3 UNDER DIFFERENT TEMPERATURE AND LIGHT REGIMES}, volume={80}, ISSN={["0031-949X"]}, DOI={10.1094/Phyto-80-309}, abstractNote={Expression of monogenic resistance in near-isogenic maize inbred lines H4460Ht1, H4460Ht2 and H4460Ht3 against isolates of races 1 and 3 of Exserohilum turcicum (Setosphaeria turcica) was determined under different temp. and light intensity regimes. These environmental conditions influenced lesion type, number, length and sporulation of the fungus. In general, isolates produced more lesions at 22/18 than at 26/22°C and more lesions at 162 or 324 than at 647 µmol m-2 s-1 at 22/18°. Lesions were also larger (P=0.05) at the reduced light intensities. Virulence of 3 races of S. turcica was clearly expressed and consistent with earlier reports at 22/18° day/night temp. and low light intensity (324 or 162 µmol m-2 s-1), but inconsistencies arose at 26/22° day/night temp. and 647 µmol m-2 s-1 light intensity. Resistance of Ht1, Ht2 and Ht3 was often incomplete at 22/18° day/night temp. and low light intensity. Race 3 could be readily recognized at a 22/18° day/night temp. regime with the differential reaction clearest at a light intensity of 324 µmol m-2 s-1}, number={3}, journal={PHYTOPATHOLOGY}, author={LEATH, S and THAKUR, RP and LEONARD, KJ}, year={1990}, month={Mar}, pages={309–313} }