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Works (4)
Updated: April 5th, 2024 11:47
2023 journal article
MitoTracker: A useful tool in need of better alternatives
EUROPEAN JOURNAL OF CELL BIOLOGY, 102(4).
author keywords: MitoTracker; Mitochondria; Fluorescent probes; Membrane potential
TL;DR:
The aim is to offer alternative dyes and discuss their advantages and disadvantages, and offer options for software with alternatives to MitoTracker to expedite future experimental design.
(via Semantic Scholar)
open access via doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 18, 2023
2020 journal article
Recurrent mismatch binding by MutS mobile clamps on DNA localizes repair complexes nearby
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 117(30), 17775–17784.
author keywords: smFRET; MMR; mismatch repair; MutS
MeSH headings : Adenosine Triphosphatases / metabolism; Adenosine Triphosphate / metabolism; Base Pair Mismatch; DNA / chemistry; DNA / genetics; DNA / metabolism; DNA Mismatch Repair; Hydrolysis; Models, Molecular; Multiprotein Complexes / metabolism; MutL Proteins / chemistry; MutL Proteins / metabolism; MutS DNA Mismatch-Binding Protein / chemistry; MutS DNA Mismatch-Binding Protein / metabolism; Structure-Activity Relationship
TL;DR:
It is revealed that after the initial mobile clamp formation event, MutS undergoes frequent cycles of mismatch rebinding and mobile clamp reformation without releasing DNA, and mechanisms that localize repair complexes to the vicinity of the mismatch are supported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 17, 2020
2018 journal article
Coordinated protein and DNA conformational changes govern mismatch repair initiation by MutS
NUCLEIC ACIDS RESEARCH, 46(20), 10782–10795.
MeSH headings : Base Pair Mismatch / genetics; Base Pairing / physiology; DNA / chemistry; DNA Mismatch Repair / genetics; Escherichia coli; Fluorescence Resonance Energy Transfer; Genomic Instability / genetics; Models, Molecular; MutS DNA Mismatch-Binding Protein / chemistry; MutS DNA Mismatch-Binding Protein / genetics; MutS DNA Mismatch-Binding Protein / metabolism; Mutant Proteins / chemistry; Mutant Proteins / metabolism; Nucleic Acid Conformation; Protein Binding / physiology; Protein Conformation; Protein Domains / genetics; Protein Isoforms / chemistry; Protein Isoforms / genetics; Protein Isoforms / metabolism
TL;DR:
A unified model of coordinated MutS and DNA conformational changes wherein initiation of mismatch repair is governed by a balance of DNA bending/unbending energetics and MutS conformationalChanges coupled to its nucleotide binding properties is proposed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 11, 2019
2018 review
Single Molecule FRET: A Powerful Tool to Study Intrinsically Disordered Proteins
[Review of ]. BIOMOLECULES, 8(4).
author keywords: single molecule biophysics; FRET; intrinsically disordered protein; IDP
MeSH headings : Fluorescence Resonance Energy Transfer; Intrinsically Disordered Proteins / chemistry; Intrinsically Disordered Proteins / metabolism; Phosphorylation; Single Molecule Imaging
TL;DR:
The power of applyingsmFRET to IDPs is illustrated by discussing two cases in the literature of protein systems for which smFRET has successfully reported phosphorylation-induced modification (but not elimination) of the disordered properties that have been connected to impacts on the related biological function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 14, 2019
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