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Works (16)
Updated: April 4th, 2024 18:07
2005 journal article
Identification of Arabidopsis thaliana transformants without selection reveals a high occurrence of silenced T-DNA integrations
PLANT JOURNAL, 41(3), 464–477.
author keywords: transcriptional gene silencing; selection bias; plant transformation; T-DNA integration; matrix attachment region; selectable marker removal
MeSH headings : Arabidopsis / genetics; Chromosome Mapping; DNA, Bacterial / genetics; Kanamycin; Mutation; Plants, Genetically Modified; Polymerase Chain Reaction; RNA Interference / physiology; Seedlings / genetics; Selection Bias; Transformation, Genetic / genetics; Transgenes / genetics
TL;DR:
The data presented here suggest that selection bias can account for at least some of the observed non-random integration of T-DNA into the Arabidopsis genome.
(via Semantic Scholar)
onlinelibrary.wiley.com (publisher PDF)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
The rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells: A flow cytometric study
PLANT CELL, 17(2), 418–429.
MeSH headings : Agrobacterium tumefaciens / genetics; DNA, Bacterial / genetics; Flow Cytometry / methods; Gene Expression Regulation, Plant; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Matrix Attachment Regions; Plants, Genetically Modified; Promoter Regions, Genetic / genetics; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Tobacco / cytology; Tobacco / genetics; Transformation, Genetic; Transgenes / genetics
TL;DR:
Flow cytometry is used to measure green fluorescent protein (GFP) expression in individual tobacco cells from lines transformed by Agrobacterium tumefaciens to conclude that MAR-mediated overall increases in transgene expression involve both likelihood and magnitude.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 journal article
An auxin-inducible gene from loblolly pine ( Pinus taeda L.) is differentially expressed in mature and juvenile-phase shoots and encodes a putative transmembrane protein
Planta, 218(6), 916–927.
author keywords: adventitious root formation; auxin; gene expression (5NG4); maturation; nodulin; Pinus
MeSH headings : Amino Acid Sequence; Arabidopsis / genetics; Down-Regulation; Gene Expression Regulation, Plant; Indoleacetic Acids / metabolism; Indoleacetic Acids / pharmacology; Molecular Sequence Data; Phylogeny; Pinus taeda / genetics; Pinus taeda / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plant Shoots / growth & development; Plant Shoots / metabolism; Sequence Alignment; Sequence Homology, Amino Acid
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
2003 journal article
Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3 ' MAR
TRANSGENIC RESEARCH, 12(1), 3–12.
author keywords: gene silencing; matrix attachment regions; transformation; transgene expression
MeSH headings : 3' Untranslated Regions / genetics; DNA Primers / chemistry; Gene Expression Regulation, Plant; Genes, Plant / physiology; Glucuronidase / genetics; Glucuronidase / metabolism; Hot Temperature; Nuclear Matrix / physiology; Plant Leaves / genetics; Plant Leaves / growth & development; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Polymerase Chain Reaction; Promoter Regions, Genetic / genetics; Tobacco / genetics; Tobacco / growth & development; Transformation, Genetic; Transgenes / physiology; Up-Regulation
TL;DR:
Most transgenes flanked by RB7 MAR elements showed a large reduction in the number of low expressing GUS transformants relative to control constructs without MARs, which is important for understanding the role of matrix attachment region in transgene expression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 journal article
E-coli chromosomal DNA in a transgene locus created by microprojectile bombardment in tobacco
TRANSGENIC RESEARCH, 11(3), 311–313.
author keywords: DNA purity; E. coli chromosomal DNA; microprojectile bombardment; transformation; transgene locus
MeSH headings : Biolistics; Escherichia coli / genetics; Plants, Genetically Modified; Tobacco / genetics; Transgenes
TL;DR:
In the process of characterising the integration site of a transgene in the tobacco genome, a 260 base pair fragment of E. coli chromosomal DNA was discovered despite the fact that standard procedures were implemented to remove contaminating E. bacteria from the plasmid DNA used in the transformation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
15. Life on Land
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2001 personal communication
Increasing loop domain size does not diminish effects of matrix attachment regions on transgene expression in tobacco cells in culture
Mendu, N., Massel, M., & Spiker, S. (2001, May 4).
MeSH headings : Biolistics; Cells, Cultured; Chromatin / metabolism; DNA, Recombinant / genetics; DNA, Recombinant / metabolism; Gene Expression; Gene Transfer Techniques; Genes, Reporter; Glucuronidase / biosynthesis; Glucuronidase / genetics; Nuclear Matrix / metabolism; Nucleosomes / metabolism; Plants, Toxic; Tobacco / cytology; Tobacco / metabolism; Transgenes
TL;DR:
It is suggested that in the previous work, the 16 nucleosomes that would be expected to form on 3 kb of DNA bounded by MARs may be below or near the minimum required to form stable, folded chromatin ¢bers in vivo, as close proximity to the nuclear matrix may sterically inhibit nucleosome formation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2001 patent
Matrix attachment regions
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions
TRANSGENIC RESEARCH, 10(5), 465–470.
author keywords: cell differentiation and proliferation; chromatin; matrix; scaffold attachment region (MAR/SAR); plant high mobility group-I/Y protein (HMG-I/Y); transgene expression; tobacco
MeSH headings : Cell Differentiation; Cells, Cultured; Gene Expression; HMGA1a Protein / genetics; HMGA1a Protein / metabolism; Plants, Genetically Modified; Tobacco / cytology; Tobacco / genetics
TL;DR:
It appears that tobacco nuclear matrix attachment region function is not enhanced simply because cells in culture divide rapidly and are not differentiated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2000 review
Use of matrix attachment regions (MARs) to minimize transgene silencing
[Review of ]. PLANT MOLECULAR BIOLOGY, 43(2-3), 361–376.
author keywords: chromatin structure; gene silencing; MAR; nuclear matrix; nuclear scaffold; SAR
MeSH headings : Animals; DNA / genetics; DNA / metabolism; Gene Expression Regulation, Plant; Gene Silencing; Humans; Nuclear Matrix / metabolism; Nuclear Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Protein Binding; Transgenes / genetics
TL;DR:
This review explores current data and presents several plausible models to explain MAR effects on transgene expression and results in higher and more stable expression in transgenic plants or cell lines.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1999 journal article
A tobacco matrix attachment region reduces the loss of transgene expression in the progeny of transgenic tobacco plants
PLANT JOURNAL, 18(3), 253–263.
TL;DR:
In primary transgenic plants, the average uidA expression in plants with MARs was twofold greater than in control plants without MARs, but there was no effect on variation of expression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1999 journal article
Characterization of randomly-obtained matrix attachment regions (MARs) from higher plants
BIOCHEMISTRY, 38(39), 12795–12804.
MeSH headings : Base Sequence; Cloning, Molecular; DNA, Plant; Molecular Sequence Data; Nuclear Matrix / metabolism; Plants / metabolism
TL;DR:
Binding strength is a function of both numbers of binding sites and affinity for the sites, and a newly identified motif, the "90%AT Box," correlates better with binding strength than any of the previously identified motifs investigated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 patent
Method of increasing expression for foreign genes in plant cells
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1998 patent
Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco
PLANT CELL, 9(8), 1251–1264.
MeSH headings : Amino Acid Sequence; Base Sequence; Chromosome Mapping; Crosses, Genetic; Cytogenetics; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; DNA, Plant / chemistry; DNA, Plant / genetics; DNA, Recombinant / chemistry; DNA, Recombinant / genetics; Gene Expression; Genes, Plant; In Situ Hybridization, Fluorescence; Molecular Biology; Molecular Sequence Data; Plants, Genetically Modified; Plants, Toxic; Sequence Homology, Amino Acid; Species Specificity; Tobacco / genetics
TL;DR:
The T-DNA structure, flanking DNA sequences, and chromosomal location of four independent transgene loci in tobacco have been determined and two of these loci were stably expressed in the homozygous condition over many generations, whereas the other two loci became unstable after several generations of homozygosity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
15. Life on Land
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1997 journal article
The tomato RBCS3A promoter requires integration into the chromatin for correct organ-specific regulation
FEBS LETTERS, 415(1), 91–95.
author keywords: chromatin; matrix attachment region; organ-specific; RBCS promoter; tomato
MeSH headings : Antigens, Nuclear; Biolistics; Cell Nucleus / metabolism; Chromatin / metabolism; DNA-Binding Proteins / analysis; Gene Expression Regulation, Plant; Genes, Plant / genetics; Genes, Reporter; Solanum lycopersicum / enzymology; Solanum lycopersicum / genetics; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Plants, Genetically Modified; Plasmids / genetics; Promoter Regions, Genetic / genetics; Protein Binding; Ribulose-Bisphosphate Carboxylase / genetics; Transformation, Genetic
TL;DR:
Fruit‐specific repression of the RBCS3A promoter was observed in transgenic plants, but not after ballistic transient transformation, indicating that chromatin integration is necessary for its correct organ‐specific regulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1996 journal article
Nuclear Matrix Attachment Regions and Transgene Expression in Plants
Plant Physiology, 110(1), 15–21.
TL;DR:
The hypothesized role of MARs in chromatin structure, how MARs are isolated and characterized, what effects MARs have had on the expression of transgenes and the models that have been evoked to explain those effects are discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
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