Steven L. Spiker
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Works (16)
Updated: July 5th, 2023 16:04
2005 journal article
Identification of Arabidopsis thaliana transformants without selection reveals a high occurrence of silenced T-DNA integrations
PLANT JOURNAL, 41(3), 464–477.
co-author countries:
United States of America 🇺🇸
author keywords: transcriptional gene silencing; selection bias; plant transformation; T-DNA integration; matrix attachment region; selectable marker removal
MeSH headings : Arabidopsis / genetics; Chromosome Mapping; DNA, Bacterial / genetics; Kanamycin; Mutation; Plants, Genetically Modified; Polymerase Chain Reaction; RNA Interference / physiology; Seedlings / genetics; Selection Bias; Transformation, Genetic / genetics; Transgenes / genetics
onlinelibrary.wiley.com (publisher PDF)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
The rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells: A flow cytometric study
PLANT CELL, 17(2), 418–429.
co-author countries:
United States of America 🇺🇸
MeSH headings : Agrobacterium tumefaciens / genetics; DNA, Bacterial / genetics; Flow Cytometry / methods; Gene Expression Regulation, Plant; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Matrix Attachment Regions; Plants, Genetically Modified; Promoter Regions, Genetic / genetics; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Tobacco / cytology; Tobacco / genetics; Transformation, Genetic; Transgenes / genetics
Sources: Web Of Science, ORCID
Added: August 6, 2018
2004 journal article
An auxin-inducible gene from loblolly pine ( Pinus taeda L.) is differentially expressed in mature and juvenile-phase shoots and encodes a putative transmembrane protein
Planta, 218(6), 916–927.
co-author countries:
United States of America 🇺🇸
author keywords: adventitious root formation; auxin; gene expression (5NG4); maturation; nodulin; Pinus
MeSH headings : Amino Acid Sequence; Arabidopsis / genetics; Down-Regulation; Gene Expression Regulation, Plant; Indoleacetic Acids / metabolism; Indoleacetic Acids / pharmacology; Molecular Sequence Data; Phylogeny; Pinus taeda / genetics; Pinus taeda / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plant Shoots / growth & development; Plant Shoots / metabolism; Sequence Alignment; Sequence Homology, Amino Acid
Sources: Web Of Science, Crossref, ORCID
Added: August 6, 2018
2003 journal article
Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3 ' MAR
TRANSGENIC RESEARCH, 12(1), 3–12.
co-author countries:
United States of America 🇺🇸
author keywords: gene silencing; matrix attachment regions; transformation; transgene expression
MeSH headings : 3' Untranslated Regions / genetics; DNA Primers / chemistry; Gene Expression Regulation, Plant; Genes, Plant / physiology; Glucuronidase / genetics; Glucuronidase / metabolism; Hot Temperature; Nuclear Matrix / physiology; Plant Leaves / genetics; Plant Leaves / growth & development; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Polymerase Chain Reaction; Promoter Regions, Genetic / genetics; Tobacco / genetics; Tobacco / growth & development; Transformation, Genetic; Transgenes / physiology; Up-Regulation
Sources: Web Of Science, ORCID
Added: August 6, 2018
2002 journal article
E-coli chromosomal DNA in a transgene locus created by microprojectile bombardment in tobacco
TRANSGENIC RESEARCH, 11(3), 311–313.
co-author countries:
United States of America 🇺🇸
author keywords: DNA purity; E. coli chromosomal DNA; microprojectile bombardment; transformation; transgene locus
MeSH headings : Biolistics; Escherichia coli / genetics; Plants, Genetically Modified; Tobacco / genetics; Transgenes
Source: Web Of Science
Added: August 6, 2018
2001 personal communication
Increasing loop domain size does not diminish effects of matrix attachment regions on transgene expression in tobacco cells in culture
Mendu, N., Massel, M., & Spiker, S. (2001, May 4).
co-author countries:
United States of America 🇺🇸
MeSH headings : Biolistics; Cells, Cultured; Chromatin / metabolism; DNA, Recombinant / genetics; DNA, Recombinant / metabolism; Gene Expression; Gene Transfer Techniques; Genes, Reporter; Glucuronidase / biosynthesis; Glucuronidase / genetics; Nuclear Matrix / metabolism; Nucleosomes / metabolism; Plants, Toxic; Tobacco / cytology; Tobacco / metabolism; Transgenes
Source: Web Of Science
Added: August 6, 2018
2001 patent
Matrix attachment regions
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions
TRANSGENIC RESEARCH, 10(5), 465–470.
co-author countries:
United States of America 🇺🇸
author keywords: cell differentiation and proliferation; chromatin; matrix; scaffold attachment region (MAR/SAR); plant high mobility group-I/Y protein (HMG-I/Y); transgene expression; tobacco
MeSH headings : Cell Differentiation; Cells, Cultured; Gene Expression; HMGA1a Protein / genetics; HMGA1a Protein / metabolism; Plants, Genetically Modified; Tobacco / cytology; Tobacco / genetics
Sources: Web Of Science, ORCID
Added: August 6, 2018
2000 review
Use of matrix attachment regions (MARs) to minimize transgene silencing
[Review of ]. PLANT MOLECULAR BIOLOGY, 43(2-3), 361–376.
co-author countries:
United States of America 🇺🇸
author keywords: chromatin structure; gene silencing; MAR; nuclear matrix; nuclear scaffold; SAR
MeSH headings : Animals; DNA / genetics; DNA / metabolism; Gene Expression Regulation, Plant; Gene Silencing; Humans; Nuclear Matrix / metabolism; Nuclear Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Protein Binding; Transgenes / genetics
Sources: Web Of Science, ORCID
Added: August 6, 2018
1999 journal article
A tobacco matrix attachment region reduces the loss of transgene expression in the progeny of transgenic tobacco plants
PLANT JOURNAL, 18(3), 253–263.
Sources: Web Of Science, ORCID
Added: August 6, 2018
1999 journal article
Characterization of randomly-obtained matrix attachment regions (MARs) from higher plants
BIOCHEMISTRY, 38(39), 12795–12804.
co-author countries:
United States of America 🇺🇸
MeSH headings : Base Sequence; Cloning, Molecular; DNA, Plant; Molecular Sequence Data; Nuclear Matrix / metabolism; Plants / metabolism
Sources: Web Of Science, ORCID
Added: August 6, 2018
1998 patent
Method of increasing expression for foreign genes in plant cells
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1998 patent
Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco
PLANT CELL, 9(8), 1251–1264.
co-author countries:
Austria 🇦🇹
MeSH headings : Amino Acid Sequence; Base Sequence; Chromosome Mapping; Crosses, Genetic; Cytogenetics; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; DNA, Plant / chemistry; DNA, Plant / genetics; DNA, Recombinant / chemistry; DNA, Recombinant / genetics; Gene Expression; Genes, Plant; In Situ Hybridization, Fluorescence; Molecular Biology; Molecular Sequence Data; Plants, Genetically Modified; Plants, Toxic; Sequence Homology, Amino Acid; Species Specificity; Tobacco / genetics
Source: Web Of Science
Added: August 6, 2018
1997 journal article
The tomato RBCS3A promoter requires integration into the chromatin for correct organ-specific regulation
FEBS LETTERS, 415(1), 91–95.
co-author countries:
Germany 🇩🇪 United States of America 🇺🇸
author keywords: chromatin; matrix attachment region; organ-specific; RBCS promoter; tomato
MeSH headings : Antigens, Nuclear; Biolistics; Cell Nucleus / metabolism; Chromatin / metabolism; DNA-Binding Proteins / analysis; Gene Expression Regulation, Plant; Genes, Plant / genetics; Genes, Reporter; Solanum lycopersicum / enzymology; Solanum lycopersicum / genetics; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Plants, Genetically Modified; Plasmids / genetics; Promoter Regions, Genetic / genetics; Protein Binding; Ribulose-Bisphosphate Carboxylase / genetics; Transformation, Genetic
Source: Web Of Science
Added: August 6, 2018
1996 journal article
Nuclear Matrix Attachment Regions and Transgene Expression in Plants
Plant Physiology, 110(1), 15–21.
co-author countries:
United States of America 🇺🇸
Sources: Web Of Science, Crossref, ORCID
Added: August 6, 2018
