Stefano Menegatti

Characterization and implications of host-cell protein aggregates in biopharmaceutical processing

Oh, Y. H., Becker, M. L., Mendola, K. M., Choe, L. H., Min, L., Lee, K. H. H., … Lenhoff, A. M. (2023, January 12). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 1.

Development of peptide affinity ligands for the purification of polyclonal and monoclonal Fabs from recombinant fluids

JOURNAL OF CHROMATOGRAPHY A, 1687.

Integrated in silico and experimental discovery of trimeric peptide ligands targeting Butyrylcholinesterase

COMPUTATIONAL BIOLOGY AND CHEMISTRY, 102.

Molecular Engineering of Cyclic Azobenzene-Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo-Affinity Chromatography

Prodromou, R., Moore, B. D., Chu, W., Deal, H., San Miguel, A., Brown, A. C., … Menegatti, S. (2023, January 25). ADVANCED FUNCTIONAL MATERIALS, Vol. 1.

Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

WENNING, C. H. U., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, February 21). (Vol. 2). Vol. 2.

De novo discovery of peptide-based affinity ligands for the fab fragment of human immunoglobulin G

JOURNAL OF CHROMATOGRAPHY A, 1669.

An accelerated antibody aggregation test based on time sequenced dynamic light scattering

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 653.

Development of peptide ligands for the purification of a-1 antitrypsin from cell culture fluids

JOURNAL OF CHROMATOGRAPHY A, 1679.

Nonwoven Membranes with Infrared Light-Controlled Permeability

Ramesh, S., Davis, J., Roros, A., Zhou, C., He, N., Gao, W., … Genzer, J. (2022, September 9). ACS APPLIED MATERIALS & INTERFACES, Vol. 9.

Potent Antibacterial Composite Nonwovens Functionalized with Bioactive Peptides and Polymers

Barbieri, E., Cutright, C. C., Ramesh, S., Khan, S. A., Efimenko, K., Genzer, J., & Menegatti, S. (2022, August 23). ADVANCED MATERIALS INTERFACES, Vol. 8.

Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes

MEMBRANES, 12(10).

Self-healing and repair of fabrics: A comprehensive review of the application toolkit

[Review of ]. MATERIALS TODAY, 54, 90–109.

Toward an Aptasensor for Monitoring of Tacrolimus

2022 IEEE SENSORS.

Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents

Sripada, S. A., Chu, W., Williams, T. I., Teten, M. A., Mosley, B. J., Carbonell, R. G., … Menegatti, S. (2022, April 22). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 4.

Design and in situ biosynthesis of precision therapies against gastrointestinal pathogens

CURRENT OPINION IN PHYSIOLOGY, 23.

Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests

PROTEOMIC PROFILING, 2 EDITION, Vol. 2261, pp. 489–506.

Dual-Responsive Microgels for Structural Repair and Recovery of Nonwoven Membranes for Liquid Filtration

ACS APPLIED POLYMER MATERIALS, 3(3), 1508–1517.

Engineering Next Generation Cyclized Peptide Ligands for Light-Controlled Capture and Release of Therapeutic Proteins

ADVANCED FUNCTIONAL MATERIALS, 31(27).

In Silico Identification and Experimental Validation of Peptide-Based Inhibitors Targeting Clostridium difficile Toxin A

Xiao, X., Sarma, S., Menegatti, S., Crook, N., Magness, S. T., & Hall, C. K. (2021, December 29). ACS CHEMICAL BIOLOGY, Vol. 12.

Peptides and pseudopeptide ligands: a powerful toolbox for the affinity purification of current and next-generation biotherapeutics

JOURNAL OF CHROMATOGRAPHY A, 1635.

Purification of polyclonal immunoglobulin G from human serum using peptide-based adsorbents

Chu, W., Sripada, S. A., Reese, H. R., Bhandari, D., Adams, A., Sly, J., … Menegatti, S. (2021, October 20). AICHE JOURNAL, Vol. 10.

Quantitative Yeast-Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein-Protein Interactions

ACS SYNTHETIC BIOLOGY, 10(3), 505–514.

Removal of host cell proteins from cell culture fluids by weak partitioning chromatography using peptide-based adsorbents

SEPARATION AND PURIFICATION TECHNOLOGY, 257.

Resorbable elastomers for implantable medical devices: highlights and applications

Turner, B., Ramesh, S., Menegatti, S., & Daniele, M. (2021, December 29). POLYMER INTERNATIONAL, Vol. 12.

Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(4).

Surface-Bound Microgels for Separation, Sensing, and Biomedical Applications

Cutright, C. C., Harris, J. L., Ramesh, S., Khan, S. A., Genzer, J., & Menegatti, S. (2021, August 16). ADVANCED FUNCTIONAL MATERIALS, Vol. 8.

Synthetic Platelet Microgels Containing Fibrin Knob B Mimetic Motifs Enhance Clotting Responses

ADVANCED THERAPEUTICS, 4(5).

Ultrasound-Powered Implants: A Critical Review of Piezoelectric Material Selection and Applications

Turner, B. L., Senevirathne, S., Kilgour, K., McArt, D., Biggs, M., Menegatti, S., & Daniele, M. A. (2021, July 8). ADVANCED HEALTHCARE MATERIALS, Vol. 7.

A multiscale coarse-grained model to predict the molecular architecture and drug transport properties of modified chitosan hydrogels

SOFT MATTER, 16(47).

Affibody-Binding Ligands

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(11).

Chitosan Hydrogels for Synergistic Delivery of Chemotherapeutics to Triple Negative Breast Cancer Cells and Spheroids

PHARMACEUTICAL RESEARCH, 37(7).

Chromatographic assay to probe the binding energy and mechanisms of homologous proteins to surface-bound ligands

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1136.

Discovery of Membrane-Permeating Cyclic Peptides via mRNA Display

BIOCONJUGATE CHEMISTRY, 31(10), 2325–2338.

Dual-Affinity Ratiometric Quenching (DARQ) Assay for the Quantification of Therapeutic Antibodies in CHO-S Cell Culture Fluids

ANALYTICAL CHEMISTRY, 92(24), 16274–16283.

Exploring the physicochemical and morphological properties of peptide‐hybridized dendrimers ( DendriPeps ) and their aggregates

Journal of Polymer Science, 58(16), 2234–2247.

Highly Efficient 1-Octene Hydroformylation at Low Syngas Pressure: From Single-Droplet Screening to Continuous Flow Synthesis

ACS CATALYSIS, 10(14), 7535–7542.

Isolation of Chemically Cyclized Peptide Binders Using Yeast Surface Display

ACS COMBINATORIAL SCIENCE, 22(10), 519–532.

Modified gaphene oxide (GO) particles in peptide hydrogels: a hybrid system enabling scheduled delivery of synergistic combinations of chemotherapeutics

JOURNAL OF MATERIALS CHEMISTRY B, 8(17), 3852–3868.

Nonwoven fiber mats with thermo-responsive permeability to inorganic and organic electrolytes

JOURNAL OF MEMBRANE SCIENCE, 616.

Novel peptide ligands for antibody purification provide superior clearance of host cell protein impurities

JOURNAL OF CHROMATOGRAPHY A, 1625.

Novel peptoid-based adsorbents for purifying IgM and IgG from polyclonal and recombinant sources

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1137.

Packing density, homogeneity, and regularity: Quantitative correlations between topology and thermoresponsive morphology of PNIPAM-co-PAA microgel coatings

APPLIED SURFACE SCIENCE, 508.

Past, Present, and Future of Affinity-based Cell Separation Technologies

[Review of ]. ACTA BIOMATERIALIA, 112, 29–51.

Peptide science: A "rule model" for new generations of peptidomimetics

[Review of ]. ACTA BIOMATERIALIA, 102, 35–74.

Photoinduced reconfiguration to control the protein-binding affinity of azobenzene-cyclized peptides

JOURNAL OF MATERIALS CHEMISTRY B, 8(33), 7413–7427.

Platelet-rich plasma lysate displays antibiofilm properties and restores antimicrobial activity against synovial fluid biofilms in vitro

JOURNAL OF ORTHOPAEDIC RESEARCH, 38(6), 1365–1374.

Purification of animal immunoglobulin G (IgG) using peptoid affinity ligands

BIOTECHNOLOGY PROGRESS.

Targeted capture of Chinese hamster ovary host cell proteins: Peptide ligand binding by proteomic analysis

BIOTECHNOLOGY AND BIOENGINEERING, 117(2), 438–452.

Use of Target-Displaying Magnetized Yeast in Screening mRNA-Display Peptide Libraries to Identify Ligands

ACS COMBINATORIAL SCIENCE, 22(12), 738–744.

Affordable Microfluidic Bead-Sorting Platform for Automated Selection of Porous Particles Functionalized with Bioactive Compounds

SCIENTIFIC REPORTS, 9(1).

DendriPeps: Expanding Dendrimer Functionality by Hybridizing Poly(amidoamine) (PAMAM) Scaffolds with Peptide Segments

MACROMOLECULAR RAPID COMMUNICATIONS, 40(22).

Discovery and Evaluation of Peptide Ligands for Selective Adsorption and Release of Cas9 Nuclease on Solid Substrates

BIOCONJUGATE CHEMISTRY, 30(12), 3057–3068.

Multiplexed Competitive Screening of One-Bead-One-Component Combinatorial Libraries Using a ClonePix 2 Colony Sorter

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(20).

Optimization of Sequence, Display, and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(1).

Screening Yeast Display Libraries against Magnetized Yeast Cell Targets Enables Efficient Isolation of Membrane Protein Binders

ACS COMBINATORIAL SCIENCE, 21(12), 817–832.

Silica resins and peptide ligands to develop disposable affinity adsorbents for antibody purification

BIOCHEMICAL ENGINEERING JOURNAL, 145, 53–61.

Tailoring the Chemical Modification of Chitosan Hydrogels to Fine-Tune the Release of a Synergistic Combination of Chemotherapeutics

BIOMACROMOLECULES, 20(8), 3126–3141.

Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(7).

Translating antibody-binding peptides into peptoid ligands with improved affinity and stability

JOURNAL OF CHROMATOGRAPHY A, 1602, 284–299.

Treating Tumors at Low Drug Doses Using an Aptamer-Peptide Synergistic Drug Conjugate

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 58(5), 1437–1441.

Purification of human erythropoietin by affinity chromatography using cyclic peptide ligands

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1085, 1–12.

Quantum dot enabled lateral flow immunoassay for detection of cardiac biomarker NT-proBNP

Sensing and Bio-Sensing Research, 21, 46–53.

A hyaluronic acid conjugate engineered to synergistically and sequentially deliver gemcitabine and doxorubicin to treat triple negative breast cancer

Journal of Controlled Release, 267, 191–202.

Design, selection, and development of cyclic peptide ligands for human erythropoietin

JOURNAL OF CHROMATOGRAPHY A, 1500, 105–120.

A combined approach for predicting the cytotoxic effect of drug-nanoaggregates

Journal of Materials Chemistry B, 4(40), 6516–6523.

DAFODIL: A novel liposome-encapsulated synergistic combination of doxorubicin and 5FU for low dose chemotherapy

JOURNAL OF CONTROLLED RELEASE, 229, 154–162.

De Novo Design of Skin-Penetrating Peptides for Enhanced Transdermal Delivery of Peptide Drugs

ADVANCED HEALTHCARE MATERIALS, 5(5), 602–609.

Design of protease-resistant peptide ligands for the purification of antibodies from human plasma

Journal of Chromatography A, 1445, 93–104.

Low-molecular-weight polymer-drug conjugates for synergistic anticancer activity of camptothecin and doxorubicin combinations

Nanomedicine, 11(9), 1139–1151.

Elasticity of Nanoparticles Influences Their Blood Circulation, Phagocytosis, Endocytosis, and Targeting

ACS Nano, 9(3), 3169–3177.

Peptides as skin penetration enhancers: Mechanisms of action

Journal of Controlled Release, 199, 168–178.

Synergistic antitumor activity of camptothecin–doxorubicin combinations and their conjugates with hyaluronic acid

Journal of Controlled Release, 210, 198–207.

Synthesis and Characterization of a Self-Fluorescent Hyaluronic Acid-Based Gel for Dermal Applications

Advanced Healthcare Materials, 4(15), 2297–2305.

Platelet-like Nanoparticles: Mimicking Shape, Flexibility, and Surface Biology of Platelets To Target Vascular Injuries

ACS Nano, 8(11), 11243–11253.

Peptide-based affinity adsorbents with high binding capacity for the purification of monoclonal antibodies

Industrial & Engineering Chemistry Research, 52(26), 8800–8811.

Reversible cyclic peptide libraries for the discovery of affinity ligands

Analytical Chemistry, 85(19), 9229–9237.

mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands

Biotechnology and Bioengineering, 110(3), 857–870.

Alkaline-stable peptide ligand affinity adsorbents for the purification of biomolecules

Journal of Chromatography A, 1245, 55–64.

Process for purification of monoclonal antibody expressed in transgenic Lemna plant extract using dextran-coated charcoal and hexamer peptide affinity resin

Journal of Chromatography A, 1260, 61–66.

Purification of polyclonal antibodies from Cohn fraction II + III, skim milk, and whey by affinity chromatography using a hexamer peptide ligand

Journal of Separation Science, 35(22), 3139–3148.

Performance of hexamer peptide ligands for affinity purification of immunoglobulin G from commercial cell culture media

Journal of Chromatography A, 1218(13), 1691–1700.