Works (105)
Updated: November 18th, 2024 15:45
2024 review
Advances and opportunities in process analytical technologies for viral vector manufacturing
[Review of ]. BIOTECHNOLOGY ADVANCES, 74.
author keywords: Biomanufacturing; Inline; At -line; Online; Viral Vectors; Gene Therapy; Process Analytical Technology; CQA; Viral Vector Characterization; Biosensors
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 8, 2024
2024 article
Factors affecting product association as a mechanism of host-cell protein persistence in bioprocessing
Oh, Y. H., Becker, M. L., Mendola, K. M., Choe, L. H., Min, L., Lee, K. H., … Lenhoff, A. M. (2024, January 19). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 1.
author keywords: dissociation constant; host-cell protein; monoclonal antibody; product association; protein A chromatography
TL;DR:
A significant role for HCP-mAb association is indicated in determining HCP persistence through protein A chromatography, presumably through binding of HCP-mAb complexes to the resin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 29, 2024
2024 journal article
Mechanistic model-based characterization of size-exclusion-mixed-mode resins for removal of monoclonal antibody fragments
Journal of Chromatography A, 1718, 464717.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
10. Reduced Inequalities
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: March 25, 2024
2024 journal article
Mixed-mode size-exclusion silica resin for polishing human antibodies in flow-through mode
Journal of Chromatography A, 1720, 464772.
author keywords: Antibody fragments; Product polishing; Mixed -mode ligands; Silica resin; Flow -through chromatography
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 29, 2024
2024 article
Multiangle Light Scattering as a Lentivirus Purification Process Analytical Technology
Sripada, S. A., Barbieri, E., Shastry, S., Wuestenhagen, E., Aldinger, A., Rammo, O., … Menegatti, S. (2024, May 28). ANALYTICAL CHEMISTRY, Vol. 5.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 11, 2024
2024 journal article
Peptide ligands for the affinity purification of adenovirus from HEK293 and vero cell lysates
JOURNAL OF CHROMATOGRAPHY A, 1736.
author keywords: Affinity chromatography; Adenovirus; Viral vectors; Gene therapy; Peptide ligands
Sources: Web Of Science, NC State University Libraries
Added: October 14, 2024
2024 article
Peptide ligands for the universal purification of exosomes by affinity chromatography
Kilgore, R. E., Moore, B. D., Sripada, S. A., Chu, W., Shastry, S., Barbieri, E., … Menegatti, S. (2024, August 4). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 8.
author keywords: affinity chromatography; bioseparations; exosomes; gene delivery; peptide ligands
open access via doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 14, 2024
2024 journal article
Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins
JOURNAL OF CHROMATOGRAPHY A, 1734.
author keywords: Gene therapy; Adeno-associated virus; Peptide ligands; Affinity chromatography; Transduction activity
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 1, 2024
2023 journal article
Biodegradable elastomeric circuit boards from citric acid-based polyesters
NPJ FLEXIBLE ELECTRONICS, 7(1).
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 19, 2023
2023 article
Breakdown of polyethylene therepthalate microplastics under saltwater conditions using engineered Vibrio natriegens
Li, T., Menegatti, S., & Crook, N. (2023, September 14). AICHE JOURNAL, Vol. 9.
author keywords: microplastics; PET biodegradation; surface display; Vibrio natriegens
TL;DR:
This project leveraged synthetic biology to develop a whole‐cell biocatalyst capable of depolymerizing PET in seawater environments by using the fast‐growing, nonpathogenic, moderate halophile Vibrio natriegens.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
11. Sustainable Cities and Communities
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
14. Life Below Water
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 15, 2023
2023 journal article
Cellulose Acetate-Stabilized Pickering Emulsions: Preparation, Rheology, and Incorporation of Agricultural Active Ingredients
ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 11(42), 15178–15191.
author keywords: nonsporulating microbes; double yielding; Pseudomonas simiae; microstructure; controlledrelease
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 1, 2023
2023 journal article
Design of 8-mer peptides that block <i>Clostridioides difficile</i> toxin A in intestinal cells
COMMUNICATIONS BIOLOGY, 6(1).
TL;DR:
An approach that combines a Pep tide B inding D esign (PepBD) algorithm, molecular-level simulations, a rapid screening assay, and surface plasmon resonance (SPR) measurements to develop peptide inhibitors that block Toxin A in colon epithelial cells is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 27, 2023
2023 article
Identification and characterization of CHO host-cell proteins in monoclonal antibody bioprocessing
Oh, Y. H., Mendola, K. M., Choe, L. H., Min, L., Lavoie, A. R., Sripada, S. A., … Lenhoff, A. M. (2023, October 25). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 10.
author keywords: bioprocessing; Chinese hamster ovary cells; host-cell proteins; monoclonal antibodies; purification
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 6, 2023
2023 journal article
Molecular Engineering of Cyclic Azobenzene‐Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo‐Affinity Chromatography
Advanced Functional Materials, 33(14).
author keywords: affinity ligands; azobenzene-peptide hybrids; biomolecular recognition; factor VIII; photo-affinity chromatography
TL;DR:
The cyclic azobenzene‐peptide ligands introduced in this report demonstrate a novel route integrating gentle operational conditions in a rapid and efficient bioprocess for the purification of life‐saving biotherapeutics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: February 20, 2023
2023 article
One-Step Quantification of anti-Covid-19 Antibodies via Dual Affinity Ratiometric Quenching Assays
Kilgour, K. M., Turner, B. L., Daniele, M., & Menegatti, S. (2023, June 27). ANALYTICAL CHEMISTRY, Vol. 6.
MeSH headings : Animals; Cricetinae; Humans; COVID-19 / diagnosis; SARS-CoV-2; CHO Cells; Cricetulus; Antibodies, Viral
TL;DR:
Evaluated the performance of the Dual-Affinity Ratiometric Quenching (DARQ) assay for the quantification of anti-SARS-CoV-2 antibodies in bioprocess harvests and intermediate fractions and human fluids indicates that the DARQ assay is a highly reproducible and rapid test, whose sensitivity, limit of detection, and dynamic range are independent of sample complexity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 10, 2023
2023 article
Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates
WENNING, C. H. U., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, February 21). (Vol. 2). Vol. 2.
Source: ORCID
Added: February 22, 2023
2023 article
Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates
Chu, W., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, July 12). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 7.
author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
TL;DR:
Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 7, 2023
2023 journal article
Peptide ligands targeting the vesicular stomatitis virus G (VSV-G) protein for the affinity purification of lentivirus particles
BIOTECHNOLOGY AND BIOENGINEERING, 121(2), 618–639.
author keywords: affinity chromatography; cell therapy; lentivirus; peptide ligands; vesicular stomatitis virus G protein
TL;DR:
This study introduces peptides targeting the enveloped protein Vesicular stomatitis virus G (VSV‐G) to serve as affinity ligands for the chromatographic purification of LV particles.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 29, 2024
2023 article
Peptonics: A new family of cell-protecting surfactants for the recombinant expression of therapeutic proteins in mammalian cell cultures
Zhang, K., Barbieri, E., Lebarre, J., Rameez, S., Mostafa, S., & Menegatti, S. (2023, October 18). BIOTECHNOLOGY JOURNAL, Vol. 10.
author keywords: antibodies; mammalian cells; Peptonics; peptoids; Pluronics; surfactants
TL;DR:
Peptonics, a novel family of peptide and peptoid surfactants whose monomer composition and sequence are designed to achieve high cell viability and productivity at a fraction of chain length and cost of Pluronics, can improve significantly purification of mAbs, thus increasing their availability at lower cost to patients.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 6, 2023
2023 journal article
Pseudo-affinity capture of K. phaffii host cell proteins in flow-through mode: Purification of protein therapeutics and proteomic study
Separation and Purification Technology, 326, 124777.
Contributors: S. Sripada n, D. Elhanafi*, L. Collins n , T. Williams n , M. Linova *, J. Woodley *, C. Boi n , n
author keywords: Komagataella phaffii; Chromatography; Protein purification; Monoclonal antibodies; Antibody fragment; Proteomics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 23, 2023
2023 journal article
Purification of a monoclonal antibody using a novel high-capacity multimodal cation exchange nonwoven membrane
Separation and Purification Technology, 317, 123920.
author keywords: Salt-tolerant multimodal ligand; Membrane adsorber; Membrane chromatography; Nonwoven membranes; Monoclonal antibodies (mAbs); High-risk HCPs
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: June 5, 2023
2023 article
Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography
Shastry, S., Chu, W., Barbieri, E., Greback-Clarke, P., Smith, W. K., Cummings, C., … Menegatti, S. (2023, September 28). BIOTECHNOLOGY JOURNAL, Vol. 9.
author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
TL;DR:
This study introduces a cohort of peptide ligands that mimic the biorecognition activity of the AAV receptor (AAVR) and anti-AAV antibody A20, enable product elution under near-physiological conditions (pH 6.0) and grant extended reusability by withstanding multiple regenerations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 10, 2023
2023 journal article
The downstream bioprocess toolbox for therapeutic viral vectors
JOURNAL OF CHROMATOGRAPHY A, 1709.
author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
TL;DR:
This study presents a comprehensive overview of the downstream bioprocess toolbox for viral vectors established in the last decade, and discusses present efforts and future directions contributing to the success of this promising class of biological medicines.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023
2023 article
Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy
2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS.
author keywords: adeno-associated virus; AAV2; immunosensor; electrochemical impedance spectroscopy; viral vector
TL;DR:
The preliminary development of an immunosensor for measuring the titer of adeno-associated virus 2 (AAV2), which may be deployed for rapid quantification of product yield during AAV biomanufacturing.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 4, 2023
2023 article
Towards electrochemical control of pH for regeneration of biosensors
2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS.
author keywords: palladium; regeneration; affinity; pH; immunosensor
TL;DR:
The development of a “solid-state” regeneration method provides significant value for extending the utility of affinity-based biosensors, such as electrochemical immunosensors and aptasensors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 4, 2023
2023 article
Vapor phase synthesis of metal-organic frameworks on a nanofibrous aerogel creates enhanced functionality
Rahmanian, V., Ebrahim, M. Z. A., Razavi, S., Abdelmigeed, M., Barbieri, E., Menegatti, S., … Khan, S. A. (2023, November 6). JOURNAL OF MATERIALS CHEMISTRY A, Vol. 11.
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 11, 2023
2022 journal article
<p>De novo discovery of peptide-based affinity ligands for the fab fragment of human immunoglobulin G</p>
JOURNAL OF CHROMATOGRAPHY A, 1669.
author keywords: Affinity ligands; Ligand design; Biorecognition; Protein chromatography; Fragment antigen binding
MeSH headings : Humans; Immunoglobulin Fab Fragments / chemistry; Immunoglobulin G; Ligands; Peptides; Tissue Distribution
TL;DR:
An integrated computational and experimental scheme is described to de novo design 9-mer peptides that bind to Fab fragments that lay the groundwork for future development of these ligands towards biomanufacturing translation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 2, 2022
2022 journal article
An accelerated antibody aggregation test based on time sequenced dynamic light scattering
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 653.
author keywords: Protein colloidal stability; Protein aggregation; Dynamic light scattering; Antibodies
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 21, 2022
2022 article
Characterization and implications of host-cell protein aggregates in biopharmaceutical processing
Oh, Y. H., Becker, M. L., Mendola, K. M., Choe, L. H., Min, L., Lee, K. H. H., … Lenhoff, A. M. (2023, January 12). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 1.
author keywords: host-cell proteins; monoclonal antibody; protein aggregation; protein purification; size-exclusion chromatography; unfolded protein response
MeSH headings : Cricetinae; Animals; Humans; Cricetulus; Protein Aggregates; Biological Products; Antibodies, Monoclonal; Proteomics / methods; CHO Cells
TL;DR:
The methods and findings described here expand the understanding of the origins and potential behavior of HCPs in cell‐based biopharmaceutical processes and may be instrumental in improving existing techniques for HCP detection and clearance.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 6, 2023
2022 journal article
Development of peptide affinity ligands for the purification of polyclonal and monoclonal Fabs from recombinant fluids
JOURNAL OF CHROMATOGRAPHY A, 1687.
author keywords: Peptide ligands; Affinity chromatography; Fragment antigen binding; Monoclonal antibodies; Cell culture harvests
MeSH headings : Cricetinae; Animals; Humans; Cricetulus; Ligands; CHO Cells; Molecular Docking Simulation; Protein Binding; Peptides / chemistry; Antibodies, Monoclonal; Chromatography, Affinity
TL;DR:
The de novo discovery of peptide ligands that target different regions of human Fab and enable product release under mild conditions are discovered and afforded values of yield and purity comparable to those provided by Protein L resins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 17, 2023
2022 journal article
Development of peptide ligands for the purification of a-1 antitrypsin from cell culture fluids
JOURNAL OF CHROMATOGRAPHY A, 1679.
author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022
2022 journal article
Integrated in silico and experimental discovery of trimeric peptide ligands targeting Butyrylcholinesterase
COMPUTATIONAL BIOLOGY AND CHEMISTRY, 102.
author keywords: Acetylcholinesterase; Butyrylcholinesterase; Purification; Molecular docking; Affinity ligands
MeSH headings : Butyrylcholinesterase / metabolism; Cholinesterase Inhibitors / chemistry; Ligands; Molecular Docking Simulation; Peptides
TL;DR:
A marked anti-correlated conformational movement governed by the ionic strength and pH of the aqueous environment is revealed, which ultimately controls BChE binding and release during chromatographic purification and provides useful guidance for ligand design and affinity maturation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 17, 2023
2022 article
Nonwoven Membranes with Infrared Light-Controlled Permeability
Ramesh, S., Davis, J., Roros, A., Zhou, C., He, N., Gao, W., … Genzer, J. (2022, September 9). ACS APPLIED MATERIALS & INTERFACES, Vol. 9.
author keywords: smart-gated membranes; photothermal effect; stimuli-responsive hydrogels; nonwovens; graphene oxide
MeSH headings : Gels; Graphite; Microgels; Permeability; Polypropylenes; Temperature
TL;DR:
This study presents the development of the first composite nonwoven fiber mats (NWFs) with infrared light-controlled permeability and shows potential toward processing temperature-sensitive bioactive ingredients or remote-controlled bioreactors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 7, 2022
2022 article
Potent Antibacterial Composite Nonwovens Functionalized with Bioactive Peptides and Polymers
Barbieri, E., Cutright, C. C., Ramesh, S., Khan, S. A., Efimenko, K., Genzer, J., & Menegatti, S. (2022, August 23). ADVANCED MATERIALS INTERFACES, Vol. 8.
author keywords: antimicrobial coating; bacitracin; controlled release; E; coli; polymyxin B; S; aureus; surface functionalization
TL;DR:
The proposed antibacterial fabric outperforms its commercial counterparts in terms of biocompatibility, showing virtually no adverse effect on human epidermal keratinocytes and demonstrate affordable and scalable routes for developing antimicrobial NWFs that efficiently eliminate resilient pathogenic bacteria.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 24, 2022
2022 journal article
Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes
Membranes, 12(10), 944.
author keywords: adeno-associated virus (AAV); viral vector purification; nonwovens; membrane adsorber; anion exchange; cation exchange; membrane chromatography
TL;DR:
A strong anion-exchange membrane was developed by UV grafting glycidyl methacrylate on a polybutylene terephthalate nonwoven followed by functionalization with triethylamine, resulting in a quaternary amine ligand (AEX-TEA membrane) that exhibited a high AAV2 binding capacity and achieved excellent DNA clearance and a recovery of infectivity higher that that reported using ion-ex exchange resin chromatography.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: November 14, 2022
2022 review
Self-healing and repair of fabrics: A comprehensive review of the application toolkit
[Review of ]. MATERIALS TODAY, 54, 90–109.
author keywords: Self-repair; Self-healing; Fabrics; Nonwovens; Property recovery; Repair
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 15, 2022
2022 article
Toward an Aptasensor for Monitoring of Tacrolimus
2022 IEEE SENSORS.
author keywords: aptamer; tacrolimus; surface plasmon resonance; square wave voltammetry
TL;DR:
A multi-aptamer system to extend the dynamic range and improve surface functionality to limit non-specific binding is explored and limited specificity for the TAC aptasensors was observed with SWV against cyclosporine, amoxicillin, and tetracycline.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 27, 2023
2022 article
Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents
Sripada, S. A., Chu, W., Williams, T. I., Teten, M. A., Mosley, B. J., Carbonell, R. G., … Menegatti, S. (2022, April 22). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 4.
Contributors: S. Sripada n, W. Chu n, T. Williams n , M. Teten n, B. Mosley n, R. Carbonell n, A. Lenhoff *, S. Cramer *
author keywords: CHO; flow-through chromatography; host cell proteins; monoclonal antibodies; peptide-based adsorbents
MeSH headings : Animals; Antibodies, Monoclonal / chemistry; CHO Cells; Cell Culture Techniques; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Peptides / chemistry; Proteomics / methods
TL;DR:
An ensemble of peptide ligands that target the HCPs in Chinese hamster ovary (CHO) cell culture fluids and enable mAb purification via flow‐through affinity chromatography are introduced and their integration into LigaGuard™, an affinity adsorbent featuring an equilibrium binding capacity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 10, 2022
2021 journal article
Design and in situ biosynthesis of precision therapies against gastrointestinal pathogens
CURRENT OPINION IN PHYSIOLOGY, 23.
TL;DR:
Recent progress in understanding of small molecules that inhibit specific virulence mechanisms point to an emerging integrative approach to treatment of gastrointestinal diseases, comprising microbiologists, peptide chemists, and synthetic biologists.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 26, 2021
2021 article
Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests
PROTEOMIC PROFILING, 2 EDITION, Vol. 2261, pp. 489–506.
Contributors: R. Lavoie n, T. Williams n , R. Blackburn *, R. Carbonell n & n
author keywords: Proteomics; Mass spectrometry; Host cell proteins; Monoclonal antibodies; Peptide ligands; Flow-through chromatography
MeSH headings : Animals; CHO Cells; Cricetulus; Enzyme-Linked Immunosorbent Assay; Humans; Ligands; Microscopy, Fluorescence; Peptide Library; Peptides / metabolism; Protein Binding; Proteins / isolation & purification; Proteins / metabolism; Proteomics; Solid Phase Extraction; Tandem Mass Spectrometry
TL;DR:
This work describes the development of HCP-binding peptide ligands, especially focusing on the steps of peptide selection via library screening and quantification of H CP removal via proteomics by mass spectrometry.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 8, 2021
2021 journal article
Dual-Responsive Microgels for Structural Repair and Recovery of Nonwoven Membranes for Liquid Filtration
ACS APPLIED POLYMER MATERIALS, 3(3), 1508–1517.
author keywords: stimuli-responsive; microgels; nonwovens; permeability; healing; fabric repair
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 13, 2021
2021 journal article
Engineering Next Generation Cyclized Peptide Ligands for Light-Controlled Capture and Release of Therapeutic Proteins
ADVANCED FUNCTIONAL MATERIALS, 31(27).
author keywords: azobenzene; cyclized peptide; light‐ controlled biorecognition; microfluidic devices; photochromic switch; photo‐ isomerization kinetics
TL;DR:
A framework for prototyping photo‐affinity adsorbents comprising azobenzene‐cyclized peptides (ACPs) conjugated to translucent porous beads (ChemMatrix) and it is demonstrated that the isomerization at different light intensities is correlated to the cyclization geometry.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 24, 2021
2021 article
In Silico Identification and Experimental Validation of Peptide-Based Inhibitors Targeting Clostridium difficile Toxin A
Xiao, X., Sarma, S., Menegatti, S., Crook, N., Magness, S. T., & Hall, C. K. (2021, December 29). ACS CHEMICAL BIOLOGY, Vol. 12.
MeSH headings : Bacterial Toxins / antagonists & inhibitors; Bacterial Toxins / chemistry; Computer Simulation; Drug Design; Enterotoxins / antagonists & inhibitors; Enterotoxins / chemistry; Models, Molecular; Peptides / chemistry; Peptides / pharmacology; Protein Binding; Protein Conformation; Protein Domains; Reproducibility of Results
TL;DR:
This work used a computational strategy that integrates a peptide binding design algorithm and explicit-solvent atomistic molecular dynamics simulation to determine promising toxin A-targeting peptides that can recognize and bind to the catalytic site of the TcdA glucosyltransferase domain (GTD).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 30, 2021
2021 article
Purification of polyclonal immunoglobulin G from human serum using peptide-based adsorbents
Chu, W., Sripada, S. A., Reese, H. R., Bhandari, D., Adams, A., Sly, J., … Menegatti, S. (2021, October 20). AICHE JOURNAL, Vol. 10.
author keywords: affinity ligands; LigaGuard (TM); LigaTrap (TM); polyclonal antibodies; protein chromatography
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 1, 2021
2021 journal article
Quantitative Yeast-Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein-Protein Interactions
ACS SYNTHETIC BIOLOGY, 10(3), 505–514.
author keywords: yeast two-hybrid; protein-protein interactions; protein engineering; yeast surface display; post-translational modification; cDNA library
MeSH headings : Gene Library; Genes, Reporter; Protein Binding; Protein Domains; Protein Interaction Maps; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Smad3 Protein / chemistry; Smad3 Protein / metabolism; Transcription Factors / chemistry; Transcription Factors / metabolism; Two-Hybrid System Techniques
TL;DR:
A quantitative yeast-yeast two-hybrid system that not only enables the discovery of specific protein-protein interactions but also efficient, quantitative estimation of their binding affinities (KD), and it was shown that qYY2H could also quantitatively evaluate binding interactions mediated by post-translational modifications on the bait protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 29, 2021
2021 article
Resorbable elastomers for implantable medical devices: highlights and applications
Turner, B., Ramesh, S., Menegatti, S., & Daniele, M. (2021, December 29). POLYMER INTERNATIONAL, Vol. 12.
author keywords: elastomers; implantable medical devices; resorbable; bioelectronics
Sources: Web Of Science, NC State University Libraries
Added: January 10, 2022
2021 journal article
Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(4).
author keywords: yeast display library; cyclic peptide; Yes Associated Protein (YAP) 65; WW domain; transglutaminase
MeSH headings : Adaptor Proteins, Signal Transducing / chemistry; Adaptor Proteins, Signal Transducing / metabolism; Albumins / metabolism; Binding Sites; Combinatorial Chemistry Techniques; Endoplasmic Reticulum / metabolism; Flow Cytometry; Ligands; Muramidase / metabolism; Peptides, Cyclic / isolation & purification; Peptides, Cyclic / pharmacology; Protein Binding; Protein Engineering / methods; Transcription Factors / chemistry; Transcription Factors / metabolism; Transglutaminases / metabolism; YAP-Signaling Proteins; Yeasts / genetics; Yeasts / growth & development
TL;DR:
The usefulness of yeast surface display for screening transglutaminase-cyclized peptide libraries, and efficient identification of cyclic peptide ligands, are demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: March 22, 2021
2021 article
Surface-Bound Microgels for Separation, Sensing, and Biomedical Applications
Cutright, C. C., Harris, J. L., Ramesh, S., Khan, S. A., Genzer, J., & Menegatti, S. (2021, August 16). ADVANCED FUNCTIONAL MATERIALS, Vol. 8.
author keywords: deposition techniques; hydrogel microparticles; stimuli-responsive polymers; surface coating; surface morphology
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 17, 2021
2021 journal article
Synthetic Platelet Microgels Containing Fibrin Knob B Mimetic Motifs Enhance Clotting Responses
Advanced Therapeutics, 4(5).
author keywords: bleeding; Clotting; fibrin; hemostasis; microgel
TL;DR:
Soft microgels decorated with the peptide AHRPYAAK are found to significantly increase clot density in vitro and decrease bleeding in a rodent trauma model in vivo, indicating that FAMCYs are capable of recapitulating the platelet‐mimetic properties of previous designs while utilizing a less costly, more translational design.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 5, 2021
2021 article
Ultrasound-Powered Implants: A Critical Review of Piezoelectric Material Selection and Applications
Turner, B. L., Senevirathne, S., Kilgour, K., McArt, D., Biggs, M., Menegatti, S., & Daniele, M. A. (2021, July 8). ADVANCED HEALTHCARE MATERIALS, Vol. 7.
author keywords: energy harvesting; implantable medical devices; piezoelectric devices; ultrasound; wireless power transfer
MeSH headings : Electric Power Supplies; Movement; Prostheses and Implants; Ultrasonography
TL;DR:
Among the various forms of WPT, ultrasound powering offers distinct advantages such as low tissue‐mediated attenuation, a higher approved safe dose (720 mW cm−2), and improved efficiency at smaller device sizes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 19, 2021
2020 journal article
A multiscale coarse-grained model to predict the molecular architecture and drug transport properties of modified chitosan hydrogels
SOFT MATTER, 16(47).
MeSH headings : Chitosan; Doxorubicin; Drug Liberation; Hydrogels; Polymers
TL;DR:
A multiscale coarse-grained model is presented to investigate the structure of networks of chitosan chains modified with acetyl, butanoyl, or heptanoysl moieties, as well as the diffusion of drugs doxorubicin and gemcitabine through the resulting networks.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: January 4, 2021
2020 journal article
Affibody-Binding Ligands
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(11).
author keywords: affibody; peptide ligands; affinity chromatography; biomanufacturing; protein purification
MeSH headings : Amino Acid Sequence; Humans; Immunoglobulin G / metabolism; Ligands; Molecular Docking Simulation; Peptide Library; Peptides / chemistry; Peptides / metabolism; Recombinant Fusion Proteins / metabolism; Serum Albumin, Human / metabolism; Temperature
TL;DR:
This work presents the development of affibody-binding peptides to be utilized as ligands for their purification from bacterial lysates and demonstrates that IGKQRI can be employed as ligand in affibODY purification processes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: July 20, 2020
2020 journal article
Chitosan Hydrogels for Synergistic Delivery of Chemotherapeutics to Triple Negative Breast Cancer Cells and Spheroids
PHARMACEUTICAL RESEARCH, 37(7).
MeSH headings : Acetylation; Antineoplastic Combined Chemotherapy Protocols / administration & dosage; Antineoplastic Combined Chemotherapy Protocols / chemistry; Antineoplastic Combined Chemotherapy Protocols / pharmacology; Cell Line, Tumor; Cell Survival / drug effects; Chitosan / chemistry; Deoxycytidine / administration & dosage; Deoxycytidine / analogs & derivatives; Deoxycytidine / chemistry; Deoxycytidine / pharmacology; Doxorubicin / administration & dosage; Doxorubicin / chemistry; Doxorubicin / pharmacology; Drug Carriers; Drug Compounding; Drug Liberation; Drug Synergism; Female; Humans; Hydrogels; Kinetics; Spheroids, Cellular; Triple Negative Breast Neoplasms / drug therapy; Triple Negative Breast Neoplasms / pathology; Gemcitabine
TL;DR:
The ACS hydrogel delivery system accomplishes drug release kinetics and molar ratio that affords strong therapeutically synergism and provides a strong pre-clinical demonstration of the potential of the proposed system for complementing surgical resection of aggressive solid tumors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 10, 2020
2020 journal article
Discovery of Membrane-Permeating Cyclic Peptides via mRNA Display
BIOCONJUGATE CHEMISTRY, 31(10), 2325–2338.
MeSH headings : Animals; Cell Line; Cell-Penetrating Peptides / chemistry; Cell-Penetrating Peptides / genetics; Cell-Penetrating Peptides / pharmacokinetics; Drosophila melanogaster / embryology; Gene Library; Humans; Mice; Models, Molecular; NIH 3T3 Cells; Peptides, Cyclic / chemistry; Peptides, Cyclic / genetics; Peptides, Cyclic / pharmacokinetics; Permeability; RNA, Messenger / genetics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 21, 2020
2020 journal article
Dual-Affinity Ratiometric Quenching (DARQ) Assay for the Quantification of Therapeutic Antibodies in CHO-S Cell Culture Fluids
ANALYTICAL CHEMISTRY, 92(24), 16274–16283.
MeSH headings : Animals; Antibodies, Monoclonal / analysis; Antibodies, Monoclonal / immunology; Antibodies, Monoclonal / therapeutic use; Antigens / immunology; CHO Cells; Cricetulus; Spectrometry, Fluorescence / methods
TL;DR:
A novel dual-affinity ratiometric quenching (DARQ) assay, which combines selective biorecognition andQuenching of fluorescence signals for rapid and sensitive quantification of therapeutic monoclonal antibodies (mAbs).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 11, 2021
2020 journal article
Exploring the physicochemical and morphological properties of peptide‐hybridized dendrimers ( DendriPeps ) and their aggregates
Journal of Polymer Science, 58(16), 2234–2247.
author keywords: dendrimets; DendriPeps; molecular dynamics; PAMAM; reversible aggregation
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: July 27, 2020
2020 journal article
Highly Efficient 1-Octene Hydroformylation at Low Syngas Pressure: From Single-Droplet Screening to Continuous Flow Synthesis
ACS CATALYSIS, 10(14), 7535–7542.
author keywords: hydroformylation; flow chemistry; BISBI; continuous organic synthesis; membrane flow reactor
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 17, 2020
2020 journal article
Isolation of Chemically Cyclized Peptide Binders Using Yeast Surface Display
ACS COMBINATORIAL SCIENCE, 22(10), 519–532.
author keywords: cyclic peptides; interleukin-17 (IL-17); library screening; yeast-display libraries; affinity ligands
MeSH headings : Biophysical Phenomena; Cell Membrane / chemistry; Cyclization; Flow Cytometry; Humans; Interleukin-17 / chemistry; Molecular Docking Simulation; Peptide Library; Peptides, Cyclic / isolation & purification; Protein Binding; Protein Conformation; Protein Engineering; Recombinant Proteins / chemistry; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / ultrastructure; Structure-Activity Relationship; Surface Properties
TL;DR:
The results show that yeast surface display can be used to efficiently isolate and characterize cyclic peptides generated by chemical modification from combinatorial libraries.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 9, 2020
2020 journal article
Modified gaphene oxide (GO) particles in peptide hydrogels: a hybrid system enabling scheduled delivery of synergistic combinations of chemotherapeutics
JOURNAL OF MATERIALS CHEMISTRY B, 8(17), 3852–3868.
MeSH headings : Antibiotics, Antineoplastic / chemistry; Antibiotics, Antineoplastic / pharmacology; Cell Proliferation / drug effects; Cell Survival / drug effects; Doxorubicin / chemistry; Doxorubicin / pharmacology; Drug Delivery Systems; Drug Screening Assays, Antitumor; Graphite / chemistry; Humans; Hydrogels / chemistry; Molecular Dynamics Simulation; Particle Size; Peptides / chemistry; Surface Properties; Tumor Cells, Cultured
TL;DR:
A homogeneous composite comprising modified graphene oxide (GO) nanoparticles embedded in a Max8 peptide hydrogel, which provides controlled kinetics and molar ratios of release of doxorubicin (DOX) and gemcitabine (GEM) and is tested against a triple negative breast cancer cell line.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 1, 2020
2020 journal article
Nonwoven fiber mats with thermo-responsive permeability to inorganic and organic electrolytes
JOURNAL OF MEMBRANE SCIENCE, 616.
author keywords: Stimuli-responsive hydrogels; "Smart gating" membranes; Stimuli-controlled permeability; Fiber coating; Controlled transport
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 12, 2020
2020 journal article
Novel peptide ligands for antibody purification provide superior clearance of host cell protein impurities
JOURNAL OF CHROMATOGRAPHY A, 1625.
author keywords: Peptide affinity ligands; Protein A; mAb purification; Affinity chromatography; Peptide design
MeSH headings : Amino Acid Sequence; Animals; Antibodies, Monoclonal / isolation & purification; Antibodies, Monoclonal / metabolism; CHO Cells; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Ligands; Peptides / chemical synthesis; Peptides / chemistry; Peptides / metabolism; Protein Binding; Staphylococcal Protein A / chemistry; Staphylococcal Protein A / metabolism
TL;DR:
WQRHGI-WorkBeads resin was finally characterized by purification of a monoclonal antibody from a Chinese Hamster Ovary cell culture harvest, affording a remarkable HCP LRV of 2.7, and consistent product yield and purity over 100 chromatographic cycles.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 10, 2020
2020 review
Past, Present, and Future of Affinity-based Cell Separation Technologies
[Review of ]. ACTA BIOMATERIALIA, 112, 29–51.
author keywords: Cell purification; Immunoaffinity; MACS; FACS; Microfluidics
MeSH headings : Cell Separation; Chromatography, Affinity; Flow Cytometry; Ligands; Regenerative Medicine
TL;DR:
This review discusses past and current methods for developing cell-targeting affinity ligands and their application in cell purification, along with the benefits and challenges associated with different purification formats, and presents new technologies towards improving the viability and throughput of cell products for tissue engineering and regenerative medicine.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
9. Industry, Innovation and Infrastructure
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 3, 2020
2020 journal article
Peptides and pseudopeptide ligands: a powerful toolbox for the affinity purification of current and next-generation biotherapeutics
JOURNAL OF CHROMATOGRAPHY A, 1635.
author keywords: Affinity ligands; peptides; peptoids; pseudopeptides; biotherapeutics
MeSH headings : Antibodies / isolation & purification; Biological Products / isolation & purification; Chemistry, Pharmaceutical / methods; Chromatography, Affinity; Family Characteristics; Humans; Ligands; Peptides / isolation & purification; Peptoids / chemistry; Proteins / isolation & purification
TL;DR:
This work presents a comprehensive and systematic review of the literature on peptide-based ligands and their use in the affinity purification of established and upcoming biological drugs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 25, 2021
2020 journal article
Photoinduced reconfiguration to control the protein-binding affinity of azobenzene-cyclized peptides
JOURNAL OF MATERIALS CHEMISTRY B, 8(33), 7413–7427.
MeSH headings : Amino Acid Sequence; Azo Compounds / chemistry; Hydrogen-Ion Concentration; Isomerism; Osmolar Concentration; Peptides, Cyclic / chemistry; Photochemical Processes
TL;DR:
This study demonstrates a general method to develop azobenzene-cyclized peptide ligands with light-controlled affinity for target proteins by designing photo-switchable peptides targeting vascular cell adhesion marker 1 (VCAM1), a cell marker implicated in stem cell function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 14, 2020
2020 journal article
Platelet-rich plasma lysate displays antibiofilm properties and restores antimicrobial activity against synovial fluid biofilms in vitro
JOURNAL OF ORTHOPAEDIC RESEARCH, 38(6), 1365–1374.
author keywords: biofilms; equine model; infectious arthritis; platelet-rich plasma; Staphylococcus aureus
MeSH headings : Aminoglycosides / pharmacology; Animals; Anti-Bacterial Agents / pharmacology; Arthritis, Infectious / drug therapy; Biofilms / drug effects; Female; Horses; Male; Molecular Weight; Platelet-Rich Plasma; Synovial Fluid / microbiology
TL;DR:
Overall, PRP‐L exhibited synergism with amikacin against aminoglycoside tolerant biofilm aggregates with greater activity against gram‐positive bacteria, which could lead to a decrease in morbidity and mortality associated with infectious arthritis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 27, 2020
2020 article
Purification of animal immunoglobulin G (IgG) using peptoid affinity ligands
BIOTECHNOLOGY PROGRESS.
MeSH headings : Animals; Chickens; Chromatography, Affinity; Goats; Immunoglobulin G / chemistry; Immunoglobulin G / immunology; Immunoglobulin G / isolation & purification; Immunoglobulins / chemistry; Immunoglobulins / isolation & purification; Ligands; Mice; Peptoids / chemistry; Protein Binding / drug effects; Rabbits; Sheep
TL;DR:
The peptoid PL16, previously isolated from an ensemble of peptoid variants of the IgG-binding peptide HWRGWV, was utilized in this work as affinity ligand on WorkBeads resin for the purification of immunoglobulin G from a variety of mammalian sources and chicken immunoglobeulin Y (IgY).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 31, 2020
2020 journal article
Removal of host cell proteins from cell culture fluids by weak partitioning chromatography using peptide-based adsorbents
SEPARATION AND PURIFICATION TECHNOLOGY, 257.
Contributors: R. Lavoie n, W. Chu n, J. Lavoie n, Z. Hetzler n, T. Williams n , R. Carbonell n, n
author keywords: Host cell proteins; Monoclonal antibodies; Flow-through chromatography; Weak partitioning mode; Polishing; Proteomics; Peptide ligands
TL;DR:
Proteomic analysis of the effluents demonstrated that 6HP+4MP-Toyopearl adsorbent removes HCPs known for their immunogenicity or IgG co-elution or degradation, demonstrating the potential of these peptide-based resins as HCP scrubbers in mAb purification processes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 11, 2021
2020 journal article
Use of Target-Displaying Magnetized Yeast in Screening mRNA-Display Peptide Libraries to Identify Ligands
ACS COMBINATORIAL SCIENCE, 22(12), 738–744.
author keywords: yeast surface display; yeast magnetization; peptide ligands; mRNA-display library; ligand discovery
MeSH headings : Ligands; Mitochondrial Membrane Transport Proteins / chemistry; Models, Molecular; Peptide Library; RNA, Messenger / chemistry; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae Proteins / chemistry; Transcription Factors / chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 11, 2021
2019 journal article
Affordable Microfluidic Bead-Sorting Platform for Automated Selection of Porous Particles Functionalized with Bioactive Compounds
SCIENTIFIC REPORTS, 9(1).
MeSH headings : Combinatorial Chemistry Techniques / instrumentation; Humans; Image Processing, Computer-Assisted; Immunoglobulin G / chemistry; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques / instrumentation; Peptide Library; Porosity; Proof of Concept Study; Unsupervised Machine Learning
TL;DR:
A low-cost microfluidics-based platform enabling automated screening of small porous beads from solid-phase peptide libraries with high sensitivity and specificity, to identify leads with high binding affinity for a biological target is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 28, 2019
2019 journal article
Chromatographic assay to probe the binding energy and mechanisms of homologous proteins to surface-bound ligands
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1136.
author keywords: Peptide ligands; IgG subclasses; pISep dual pH and salt LC gradients; Isothermal titration calorimetry; In silico docking, IgG peptide interaction forces
MeSH headings : Adsorption; Calorimetry; Chromatography, Affinity / methods; Humans; Hydrogen-Ion Concentration; Immunoglobulin G / chemistry; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Ligands; Molecular Dynamics Simulation; Peptides / chemistry; Peptides / metabolism; Protein Binding; Recombinant Proteins / chemistry; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sodium Chloride / chemistry; Static Electricity
TL;DR:
The effectiveness of the pISep toolbox for probing the differential binding of homologous proteins to a reference ligand and informing the optimization of platform processes for the purification and fractionation of biotherapeutics is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: February 17, 2020
2019 journal article
DendriPeps: Expanding Dendrimer Functionality by Hybridizing Poly(amidoamine) (PAMAM) Scaffolds with Peptide Segments
MACROMOLECULAR RAPID COMMUNICATIONS, 40(22).
author keywords: internally functionalized dendrimers; orthogonal protection strategies; poly(amidoamine); polymer-peptide chemistry
MeSH headings : Amino Acids / chemistry; Dendrimers / chemistry; Dendrimers / metabolism; Drug Delivery Systems / methods; Molecular Dynamics Simulation; Molecular Structure; Peptides / chemistry; Peptides / metabolism; Tissue Scaffolds / chemistry
TL;DR:
The precise control over the number, type, and topological placement of functional groups expands the functional behavior of DendriPeps beyond current PAMAM dendrimers toward new frontiers or colloids, drug delivery vectors, and catalysis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 14, 2019
2019 journal article
Discovery and Evaluation of Peptide Ligands for Selective Adsorption and Release of Cas9 Nuclease on Solid Substrates
BIOCONJUGATE CHEMISTRY, 30(12), 3057–3068.
MeSH headings : Adsorption; Amino Acid Sequence; Binding Sites; CRISPR-Associated Protein 9 / isolation & purification; CRISPR-Associated Protein 9 / metabolism; Drug Discovery; Ligands; Molecular Docking Simulation; Peptide Library; Peptides / metabolism; Resins, Synthetic / chemistry; Substrate Specificity
TL;DR:
This work represents the first effort at developing short synthetic Cas9-binding peptides and demonstrates their applicability as affinity ligands for the purification of a Cas nuclease.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 13, 2020
2019 journal article
Multiplexed Competitive Screening of One-Bead-One-Component Combinatorial Libraries Using a ClonePix 2 Colony Sorter
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(20).
author keywords: library screening; bioactive peptides; ligand development; affinity chromatography; affinity ligand; ClonePix 2
MeSH headings : Animals; Cell Line; Chromatography, Affinity / methods; Drug Discovery / methods; Humans; Immunoglobulin G; Ligands; Optical Imaging / methods; Peptide Library; Small Molecule Libraries
TL;DR:
This work presents a method for multiplexed solid-phase peptide library screening using a ClonePix 2 Colony Picker that integrates orthogonal fluorescent labeling for positive selection against a target protein and negative selection against competitor species with semi-quantitative tracking of target vs. competitor binding for every library bead.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 16, 2019
2019 journal article
Novel peptoid-based adsorbents for purifying IgM and IgG from polyclonal and recombinant sources
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1137.
author keywords: Peptoid ligand; Affinity purification; Protein chromatography; IgG and IgM; Antibody therapeutics
MeSH headings : Animals; CHO Cells; Chromatography, Affinity / methods; Cricetinae; Cricetulus; HEK293 Cells; Humans; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Immunoglobulin M / isolation & purification; Immunoglobulin M / metabolism; Peptoids / chemistry; Peptoids / metabolism; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism
TL;DR:
The potential of the peptoid ligand for manufacturing polyclonal IgG- and IgM-enriched formulations and monoclonal IgM therapeutics is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 10, 2020
2019 journal article
Optimization of Sequence, Display, and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(1).
author keywords: affinity chromatography; peptide ligand; antibody purification; binding capacity; process optimization; aggregate removal
TL;DR:
The use of peptide ligand HWRGWV and its cognate sequences to develop affinity adsorbents that compete with Protein A in terms of binding capacity and quality of the eluted product demonstrate the potential of peptides as alternative to Protein A for the purification of therapeutic antibodies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 11, 2019
2019 journal article
Packing density, homogeneity, and regularity: Quantitative correlations between topology and thermoresponsive morphology of PNIPAM-co-PAA microgel coatings
APPLIED SURFACE SCIENCE, 508.
author keywords: Stimuli-responsive microgels; Self-assembled monolayers; Spin coating; Particle interactions; Particle packing
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 14, 2020
2019 review
Peptide science: A "rule model" for new generations of peptidomimetics
[Review of ]. ACTA BIOMATERIALIA, 102, 35–74.
author keywords: Peptides; Peptoids; Simulation; Peptidomimetics; Biomaterials
MeSH headings : Amino Acid Sequence; Molecular Dynamics Simulation; Peptidomimetics / chemistry; Peptoids / chemistry; Protein Structure, Secondary; Protein Structure, Tertiary; Sequence Analysis, Protein
TL;DR:
Techniques to determine primary, secondary, and tertiary structure of peptides, and how they have been adapted to investigate peptoid structure are examined.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 27, 2020
2019 journal article
Screening Yeast Display Libraries against Magnetized Yeast Cell Targets Enables Efficient Isolation of Membrane Protein Binders
ACS COMBINATORIAL SCIENCE, 21(12), 817–832.
author keywords: yeast magnetization; combinatorial library screening; ligand discovery; yeast surface display; membrane protein; Sso7d; nanobody
MeSH headings : Combinatorial Chemistry Techniques; Drug Evaluation, Preclinical; Ferric Compounds / chemistry; Ferric Compounds / pharmacology; Magnetic Phenomena; Mitochondria / drug effects; Mitochondria / metabolism; Mitochondrial Membrane Transport Proteins / antagonists & inhibitors; Mitochondrial Membrane Transport Proteins / isolation & purification; Mitochondrial Membrane Transport Proteins / metabolism; Nanoparticles / chemistry; Protein Binding / drug effects; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / antagonists & inhibitors; Saccharomyces cerevisiae Proteins / isolation & purification; Saccharomyces cerevisiae Proteins / metabolism; Small Molecule Libraries / chemistry; Small Molecule Libraries / pharmacology
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 30, 2019
2019 journal article
Tailoring the Chemical Modification of Chitosan Hydrogels to Fine-Tune the Release of a Synergistic Combination of Chemotherapeutics
BIOMACROMOLECULES, 20(8), 3126–3141.
MeSH headings : Antineoplastic Combined Chemotherapy Protocols / chemistry; Antineoplastic Combined Chemotherapy Protocols / pharmacology; Breast Neoplasms / drug therapy; Chitosan / chemistry; Deoxycytidine / administration & dosage; Deoxycytidine / analogs & derivatives; Doxorubicin / administration & dosage; Drug Carriers / chemistry; Drug Liberation; Drug Synergism; Female; Humans; Hydrogels / chemistry; Polymers / chemistry; Gemcitabine
TL;DR:
A computational toolbox that models the migration of a drug pair through a hydrated network of polysaccharide chains modified with hydrophobic moieties is developed and the potential of this model in guiding the design of chemotherapeutic hydrogels to combat cancer is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 3, 2019
2019 journal article
Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(7).
author keywords: therapeutic antibodies; host cell proteins; protein purification; peptide ligands
MeSH headings : Animals; CHO Cells; Chromatography, Affinity; Chromatography, Liquid; Cricetinae; Cricetulus; Humans; Peptides / chemistry; Peptides / isolation & purification; Proteomics / methods
TL;DR:
Development of synthetic peptide ligands capable of capturing a broad spectrum of Chinese hamster ovary (CHO) HCPs with a combination of peptide species that allow for advanced mixed-mode binding is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 6, 2019
2019 journal article
Targeted capture of Chinese hamster ovary host cell proteins: Peptide ligand binding by proteomic analysis
BIOTECHNOLOGY AND BIOENGINEERING, 117(2), 438–452.
Contributors: R. Lavoie n, A. Fazio n, T. Williams n , R. Carbonell n & n
author keywords: host cell proteins; monoclonal antibodies; polishing; peptide ligands; protein purification
MeSH headings : Animals; Antibodies, Monoclonal / isolation & purification; CHO Cells; Cricetinae; Cricetulus; Ligands; Peptides / chemistry; Peptides / metabolism; Protein Binding; Proteins / analysis; Proteins / chemistry; Proteins / isolation & purification; Proteins / metabolism; Proteomics / methods; Recombinant Proteins / isolation & purification
TL;DR:
A family of synthetic peptides that target HCPs and exhibit low binding to IgG product are developed and found that these peptide ligands exhibit superior HCP binding capability compared to those of the benchmark commercial resins commonly used in mAb purification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 2, 2019
2019 journal article
Translating antibody-binding peptides into peptoid ligands with improved affinity and stability
JOURNAL OF CHROMATOGRAPHY A, 1602, 284–299.
author keywords: Peptoid ligands; Molecular docking; Affinity chromatography; Antibodies; Protein A mimetic
MeSH headings : Adsorption; Alkalies / chemistry; Amino Acid Sequence; Animals; Antibodies / metabolism; Antibody Affinity; Binding Sites; CHO Cells; Cattle; Cricetinae; Cricetulus; Humans; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Ligands; Molecular Docking Simulation; Peptides / chemistry; Peptoids / chemistry; Protein Binding; Proteolysis; Temperature
TL;DR:
This work proposes a route for translation utilizing the IgG-binding peptide HWRGWV as reference sequence and forms a method guiding the design of peptoid variants of cognate peptide ligands, and help addressing the challenges that, despite the structural similarity, the peptide-to-peptoid translation presents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 16, 2019
2018 journal article
Purification of human erythropoietin by affinity chromatography using cyclic peptide ligands
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1085, 1–12.
author keywords: Cyclic peptide ligand; Erythropoietin purification; Affinity chromatography; Chinese hamster ovary; Pichia pastoris
MeSH headings : Animals; CHO Cells; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Erythropoietin / analysis; Erythropoietin / chemistry; Erythropoietin / isolation & purification; Erythropoietin / metabolism; Humans; Hydrogen-Ion Concentration; Peptides, Cyclic / chemistry; Peptides, Cyclic / metabolism; Pichia; Proteins
TL;DR:
Results compare well with values obtained using wheat germ agglutinin agarose and clearly indicate the potential of the cyclic peptide resins as a viable tool for rHuEPO purification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2018 journal article
Quantum dot enabled lateral flow immunoassay for detection of cardiac biomarker NT-proBNP
Sensing and Bio-Sensing Research, 21, 46–53.
author keywords: NT-proBNP; cardiac biomarker; lateral flow; immunoassay; quantum dot
TL;DR:
A case study using two monoclonal antibodies for NT-proBNP, respectively, as detector and capture antibodies is presented, highlighting a method for high-resolution, low-cost quantification of NT- proBNP as a point-of-care, single-step diagnostic sensor.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2018 journal article
Silica resins and peptide ligands to develop disposable affinity adsorbents for antibody purification
BIOCHEMICAL ENGINEERING JOURNAL, 145, 53–61.
author keywords: Silica resins; Peptide ligands; Monoclonal antibodies; Llama antibodies; Rabbit antibodies
TL;DR:
It is demonstrated that porous silica, a non-traditional substrate for protein purification, shows great promise as potentially single-use affinity adsorbent forprotein purification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 13, 2019
2018 journal article
Treating Tumors at Low Drug Doses Using an Aptamer-Peptide Synergistic Drug Conjugate
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 58(5), 1437–1441.
author keywords: aptamer-drug conjugate; cancer; peptide scaffolds; synergy; targeted drug-delivery
MeSH headings : Antineoplastic Combined Chemotherapy Protocols / chemistry; Antineoplastic Combined Chemotherapy Protocols / therapeutic use; Aptamers, Nucleotide / chemistry; Camptothecin / chemistry; Camptothecin / therapeutic use; Cell Line; Cell Proliferation / drug effects; Doxorubicin / chemistry; Doxorubicin / therapeutic use; Drug Screening Assays, Antitumor; Humans; Maximum Tolerated Dose; Molecular Structure; Neoplasms / drug therapy; Neoplasms / pathology; Peptides / chemistry
TL;DR:
A modular framework has been developed that selectively delivers drug combinations at synergistic ratios via tumor-targeting aptamers for effective low-dose treatment and represents a generalizable strategy for the safe and consistent delivery of combination drugs in oncology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 4, 2019
2017 journal article
A hyaluronic acid conjugate engineered to synergistically and sequentially deliver gemcitabine and doxorubicin to treat triple negative breast cancer
Journal of Controlled Release, 267, 191–202.
author keywords: Drug combinations; Polymer drug conjugates; Synergy; Breast cancer; Gemcitabine; Doxorubcin
MeSH headings : Animals; Antibiotics, Antineoplastic / administration & dosage; Antibiotics, Antineoplastic / chemistry; Antimetabolites, Antineoplastic / administration & dosage; Antimetabolites, Antineoplastic / chemistry; Antineoplastic Combined Chemotherapy Protocols / administration & dosage; Antineoplastic Combined Chemotherapy Protocols / chemistry; Cell Line, Tumor; Cell Survival / drug effects; Deoxycytidine / administration & dosage; Deoxycytidine / analogs & derivatives; Deoxycytidine / chemistry; Dose-Response Relationship, Drug; Doxorubicin / administration & dosage; Doxorubicin / chemistry; Drug Carriers / administration & dosage; Drug Carriers / chemistry; Drug Liberation; Drug Synergism; Humans; Hyaluronic Acid / administration & dosage; Hyaluronic Acid / chemistry; Mice; Mice, Inbred BALB C; Prodrugs / administration & dosage; Prodrugs / chemistry; Triple Negative Breast Neoplasms / drug therapy; Gemcitabine
TL;DR:
It is shown that polymer conjugates that release GEM faster than DOX are more effective at killing triple negative breast cancer cells in vitro and the optimal dual drug conjugate more effectively inhibits the growth of an aggressive, orthotopic 4T1 tumor model in vivo than free DOX and GEM and the single drug HA conjugating.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2017 journal article
Design, selection, and development of cyclic peptide ligands for human erythropoietin
JOURNAL OF CHROMATOGRAPHY A, 1500, 105–120.
author keywords: Erythropoietin; Cyclic peptide ligands; Ligand screening; Affinity chromatography; Affinity maturation; In-silico docking
MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Erythropoietin / chemistry; Humans; Kinetics; Ligands; Molecular Sequence Data; Peptides, Cyclic / chemistry; Peptides, Cyclic / isolation & purification; Protein Binding
TL;DR:
Mutagenesis studies performed on FSLLSH with natural and non-natural amino acid substitutions led to the identification of critical EPO-binding determinants, and the discovery of new peptide ligands that facilitate EPO recovery.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
A combined approach for predicting the cytotoxic effect of drug-nanoaggregates
Journal of Materials Chemistry B, 4(40), 6516–6523.
TL;DR:
In silico and spectroscopic analysis indicate that released etoposide nanoaggregates have a different structure, stability, and bioactivity, which depend on the pH experienced during the release, compared to those released from dextran-etoposid conjugates.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: March 26, 2019
2016 journal article
DAFODIL: A novel liposome-encapsulated synergistic combination of doxorubicin and 5FU for low dose chemotherapy
JOURNAL OF CONTROLLED RELEASE, 229, 154–162.
author keywords: Drug combinations; Synergistic; Chemotherapy; Liposomes; Nanoparticles
MeSH headings : Animals; Antibiotics, Antineoplastic / administration & dosage; Antibiotics, Antineoplastic / chemistry; Antibiotics, Antineoplastic / pharmacokinetics; Antibiotics, Antineoplastic / therapeutic use; Antimetabolites, Antineoplastic / administration & dosage; Antimetabolites, Antineoplastic / chemistry; Antimetabolites, Antineoplastic / pharmacokinetics; Antimetabolites, Antineoplastic / therapeutic use; Cell Line; Cell Line, Tumor; Doxorubicin / administration & dosage; Doxorubicin / chemistry; Doxorubicin / pharmacokinetics; Doxorubicin / therapeutic use; Drug Combinations; Female; Fluorouracil / administration & dosage; Fluorouracil / chemistry; Fluorouracil / pharmacokinetics; Fluorouracil / therapeutic use; Humans; Liposomes; Mice, Inbred BALB C; Neoplasms / drug therapy; Neoplasms / metabolism; Neoplasms / pathology; Polyethylene Glycols / chemistry; Tryptophan / chemistry; Tumor Burden / drug effects
TL;DR:
This work demonstrates a novel PEGylated liposome system, named DAFODIL (Doxorubicin And 5-Flurouracil Optimally Delivered In a Liposome) that inarguably offers superior therapeutic efficacies compared to free drug administrations and provides a chemotherapy vehicle which can both reduce toxicity and improve therapeutic efficacy.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
De Novo Design of Skin-Penetrating Peptides for Enhanced Transdermal Delivery of Peptide Drugs
ADVANCED HEALTHCARE MATERIALS, 5(5), 602–609.
MeSH headings : Administration, Cutaneous; Adult; Amino Acid Sequence; Cell Survival / drug effects; Computer Simulation; Cyclosporine / metabolism; Cyclosporine / pharmacology; Drug Delivery Systems / methods; Drug Design; Epidermal Cells; Humans; Keratinocytes / cytology; Keratinocytes / drug effects; Keratins / metabolism; Mass Spectrometry; Peptide Library; Peptides / administration & dosage; Peptides / chemistry; Peptides / pharmacology; Peptides / toxicity; Pharmaceutical Preparations / administration & dosage; Skin / drug effects; Skin Absorption / drug effects; Thermodynamics
TL;DR:
A computational process for generating and screening virtual libraries of disulfide‐cyclic peptides against keratin and cyclosporine A (CsA) to identify SPPs capable of enhancing transdermal CsA delivery and represents an effective procedure for the de novo design of skin‐penetrating peptides for the delivery of desired therapeutic or cosmetic agents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Design of protease-resistant peptide ligands for the purification of antibodies from human plasma
Journal of Chromatography A, 1445, 93–104.
author keywords: Peptide affinity ligands; Polyclonal antibodies; Affinity chromatography; Proteolysis; Protease; Molecular docking
MeSH headings : Animals; Antibodies / isolation & purification; Binding Sites; Blood Chemical Analysis / methods; Chromatography, Affinity; Chymotrypsin / metabolism; Humans; Ligands; Peptides / chemistry; Protein Binding; Trypsin / metabolism
TL;DR:
A strategy is presented for developing variants of peptide ligands with enhanced biochemical stability for the purification of antibodies from animal sera and higher selectivity than the original sequences, due to electrical neutrality.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Low-molecular-weight polymer-drug conjugates for synergistic anticancer activity of camptothecin and doxorubicin combinations
Nanomedicine, 11(9), 1139–1151.
author keywords: combination chemotherapy; drug delivery; linker chemistry; low-molecular-weight conjugates; polymer-drug conjugates
MeSH headings : Antineoplastic Combined Chemotherapy Protocols; Camptothecin / administration & dosage; Camptothecin / chemistry; Cell Line, Tumor; Cell Proliferation / drug effects; Doxorubicin / administration & dosage; Doxorubicin / chemistry; Drug Delivery Systems; Drug Synergism; Humans; Hyaluronic Acid / chemistry; Molecular Weight; Neoplasms / drug therapy; Polymers / chemistry; Polyvinyl Alcohol / chemistry
TL;DR:
This study shows the high therapeutic potential of low MW polymer-drug conjugated to synergistic combinations of camptothecin and doxorubicin for polychemotherapy delivery, and provides further insight into the development of polymer- Drugs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Elasticity of Nanoparticles Influences Their Blood Circulation, Phagocytosis, Endocytosis, and Targeting
ACS Nano, 9(3), 3169–3177.
author keywords: elasticity; nanogel; nanoemulsion; biodistribution; circulation; targeting; nanomedicine; nanoparticles
MeSH headings : Animals; Blood / metabolism; Cell Line, Tumor; Elastic Modulus; Humans; Hydrogels / chemistry; Hydrogels / metabolism; Hydrogels / pharmacokinetics; Mice; Nanoparticles; Particle Size; Phagocytosis; Polyethylene Glycols / chemistry; Structure-Activity Relationship; Tissue Distribution
TL;DR:
Tuning nanoparticle elasticity potentially offers a method to improve the biological fate of nanoparticles by offering enhanced circulation, reduced immune system uptake, and improved targeting.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2015 journal article
Synergistic antitumor activity of camptothecin–doxorubicin combinations and their conjugates with hyaluronic acid
Journal of Controlled Release, 210, 198–207.
author keywords: Chemotherapy; Drug combinations; Polymer drug conjugate; Synergistic
MeSH headings : Animals; Antineoplastic Agents, Phytogenic / administration & dosage; Antineoplastic Agents, Phytogenic / chemistry; Antineoplastic Combined Chemotherapy Protocols / administration & dosage; Apoptosis / drug effects; Camptothecin / administration & dosage; Camptothecin / chemistry; Cell Line, Tumor; Doxorubicin / administration & dosage; Doxorubicin / chemistry; Drug Synergism; Female; Humans; Hyaluronic Acid / administration & dosage; Hyaluronic Acid / chemistry; Mice, Inbred BALB C; Neoplasms / drug therapy; Neoplasms / pathology; Topoisomerase I Inhibitors / administration & dosage; Topoisomerase I Inhibitors / chemistry; Topoisomerase II Inhibitors / administration & dosage; Topoisomerase II Inhibitors / chemistry; Tumor Burden / drug effects
TL;DR:
This study elucidates a method to identify synergistic drug combinations and translate them to in vivo by preserving the synergistic ratio via conjugation to a carrier polymer, thus opening a promising approach to translate drug combinations to clinically viable treatment regimens.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2015 journal article
Synthesis and Characterization of a Self-Fluorescent Hyaluronic Acid-Based Gel for Dermal Applications
Advanced Healthcare Materials, 4(15), 2297–2305.
MeSH headings : Animals; Biocompatible Materials / chemistry; Cell Line; Cell Proliferation / drug effects; Delayed-Action Preparations; Drug Liberation; Humans; Hyaluronic Acid / chemistry; Hydrogels / chemistry; Keratinocytes / drug effects; Keratinocytes / metabolism; Polymers; Rheology; Skin / drug effects; Skin / metabolism; Swine; Tissue Engineering
TL;DR:
The presented results demonstrate the applicability of the binary mixture for in situ gelation and the potential of the proposed system for a variety of biomedical applications and the safety of the HA‐based conjugates is demonstrated on human keratinocytes.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2014 journal article
Peptides as skin penetration enhancers: Mechanisms of action
Journal of Controlled Release, 199, 168–178.
author keywords: Cell penetrating peptide; Cyclosporine; Keratin; Transporter; Topical; Transcellular
MeSH headings : Cells, Cultured; Computer Simulation; Cyclosporine / administration & dosage; Cyclosporine / pharmacokinetics; Drug Carriers / adverse effects; Drug Carriers / chemistry; Drug Carriers / pharmacology; Humans; Keratinocytes / drug effects; Keratins / metabolism; Lipids / chemistry; Peptides / adverse effects; Peptides / chemistry; Peptides / pharmacology; Skin / chemistry; Skin / drug effects; Skin Absorption / drug effects; Spectroscopy, Fourier Transform Infrared; Structure-Activity Relationship; Water Loss, Insensible / drug effects
TL;DR:
Among the peptides tested, SPACE peptide was found to be least toxic to keratinocytes, and among the most effective at delivering CsA into the skin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2014 journal article
Platelet-like Nanoparticles: Mimicking Shape, Flexibility, and Surface Biology of Platelets To Target Vascular Injuries
ACS Nano, 8(11), 11243–11253.
author keywords: platelets; synthetic cells; layer-by-layer; nanotechnology; hemostat; vascular targeting
MeSH headings : Animals; Blood Platelets / pathology; Cell Adhesion; Cell Shape; Female; Mice; Mice, Inbred BALB C; Microscopy, Electron, Scanning; Nanoparticles; Platelet Aggregation; Spectroscopy, Fourier Transform Infrared; Vascular System Injuries / pathology
TL;DR:
Platelet-like nanoparticles (PLNs) offer a nanoscale technology that integrates platelet-mimetic biophysical and biochemical properties for potential applications in injectable synthetic hemostats and vascularly targeted payload delivery.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2013 journal article
Reversible cyclic peptide libraries for the discovery of affinity ligands
Analytical Chemistry, 85(19), 9229–9237.
MeSH headings : Ligands; Molecular Structure; Peptide Library; Peptides, Cyclic / analysis; Tandem Mass Spectrometry
TL;DR:
A method is shown for synthesis of the irreversible cyclic peptide corresponding to the proposed depsipeptide structure, to make the ligand stable to the aqueous acid and alkaline conditions encountered in affinity chromatographic applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Alkaline-stable peptide ligand affinity adsorbents for the purification of biomolecules
Journal of Chromatography A, 1245, 55–64.
author keywords: Peptide ligand; Affinity adsorbent; NaOH stability; Modification of surface chemistry; Solid-phase peptide synthesis; Cleaning-in-place
MeSH headings : Adsorption; Alkalies / chemistry; Chromatography, Affinity / instrumentation; Chromatography, Affinity / methods; Immunoglobulin G / chemistry; Immunoglobulin G / isolation & purification; Ligands; Peptides / chemistry; Protein Binding
TL;DR:
It was found that the ligand leaching was caused by the coupling of a significant amount of peptide by alkaline-labile ester bonds, and the method presented has a broad application since it is valid for any peptide ligand identified for the purification of a biopharmaceutical target.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Peptide-based affinity adsorbents with high binding capacity for the purification of monoclonal antibodies
Industrial & Engineering Chemistry Research, 52(26), 8800–8811.
TL;DR:
The development of affinity resins based on hexapeptide ligand HWRGWV for recovering monoclonal antibodies from cell culture fluids and the reaction conditions of peptide coupling were optimized to maximize the binding capacity of the resulting adsorbents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Process for purification of monoclonal antibody expressed in transgenic Lemna plant extract using dextran-coated charcoal and hexamer peptide affinity resin
Journal of Chromatography A, 1260, 61–66.
author keywords: Dextran-coated charcoal; Transgenic plants; Hexapeptide ligand; IgG purification; Monoclonal antibody; Phenolics; Lemna minor
MeSH headings : Antibodies, Monoclonal / isolation & purification; Antibodies, Monoclonal / metabolism; Araceae / chemistry; Araceae / metabolism; Charcoal / chemistry; Chromatography, Affinity / methods; Dextrans / chemistry; Humans; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Peptides / chemistry; Peptides / metabolism; Plant Extracts / chemistry; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / metabolism; Protein Binding; Staphylococcal Protein A / chemistry; Staphylococcal Protein A / isolation & purification
TL;DR:
The development of a pretreatment and affinity separation process for the purification of a monoclonal antibody from transgenic Lemna plant extract and the performance of the hexamer peptide resin after the pretreatment step was found to be similar to that obtained with a commercial Protein A resin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Purification of polyclonal antibodies from Cohn fraction II + III, skim milk, and whey by affinity chromatography using a hexamer peptide ligand
Journal of Separation Science, 35(22), 3139–3148.
author keywords: Affinity chromatography; Immunoglobulin G; Milk; Peptide ligands; Plasma
MeSH headings : Animals; Antibodies / chemistry; Antibodies / isolation & purification; Blood Proteins / chemistry; Blood Proteins / isolation & purification; Cattle; Chromatography, Affinity / instrumentation; Chromatography, Affinity / methods; Humans; Ligands; Milk / chemistry; Milk Proteins / chemistry; Milk Proteins / isolation & purification; Peptides / chemistry; Whey Proteins
TL;DR:
HWRGWV, a peptide that binds specifically to the Fc fragment of human immunoglobulin G (IgG), was used for the purification of IgG from Cohn fraction II + III of human plasma and from bovine skim milk and whey.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2012 journal article
mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands
Biotechnology and Bioengineering, 110(3), 857–870.
author keywords: cyclic peptide; mRNA display; Fc binding; affinity ligand; library
MeSH headings : Humans; Immunoglobulin Fc Fragments / metabolism; Peptide Library; Peptides, Cyclic / chemistry; Peptides, Cyclic / genetics; Peptides, Cyclic / isolation & purification; Peptides, Cyclic / metabolism; Protein Binding; Protein Engineering / methods; RNA, Messenger / genetics; Solid-Phase Synthesis Techniques / methods
TL;DR:
The discovery, synthesis and characterization of novel cyclic peptide affinity ligands that bind the Fc portion of human Immunoglobulin G (IgG; hFc) are described and the approach can be extended to efficiently generate and evaluatecyclic peptides for other targets of interest.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Performance of hexamer peptide ligands for affinity purification of immunoglobulin G from commercial cell culture media
Journal of Chromatography A, 1218(13), 1691–1700.
author keywords: Hexapeptide ligand; IgG purification; Monoclonal antibody; Affinity chromatography; Regeneration
MeSH headings : Adsorption; Animals; Antibodies, Monoclonal / chemistry; Antibodies, Monoclonal / isolation & purification; CHO Cells; Caprylates; Cell Culture Techniques; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Culture Media; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin G / chemistry; Immunoglobulin G / isolation & purification; Oligopeptides / chemistry; Phosphoric Acids; Protein Binding; Sodium Chloride; Urea
TL;DR:
Issues related to the successful application of the hexapeptide ligands, on a commercial methacrylate chromatographic resin, for the purification of IgG from mammalian cell culture fluids are addressed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
Employment
Updated: November 14th, 2018 12:31
2015 - present
North Carolina State University Raleigh, NC, US
Assistant Professor
Chemical and Biomolecular Engineering
Education
Updated: November 14th, 2018 12:33
2009 - 2013
North Carolina State University Raleigh, NC, US
Ph.D.
Chemical and Biomolecular Engineering
2006 - 2008
Università degli Studi di Bologna Bologna, Emilia-Romagna, IT
M.Sc.
Ingegneria Chimica
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