@article{fogarty_smith_sheridan_hu_islamovic_reid_jackson_maughan_ames_jellen_et al._2020, title={Identification of mixed linkage beta-glucan quantitative trait loci and evaluation of AsCslF6 homoeologs in hexaploid oat}, volume={60}, ISSN={["1435-0653"]}, DOI={10.1002/csc2.20015}, abstractNote={AbstractHexaploid oat (Avena sativa L.) is a commercially important cereal crop due in part to (1‐3,1‐4)‐β‐D‐glucan (β‐glucan), a hemicellulose important to human health. Cellulose synthase‐like (Csl) genes have been shown to contribute to β‐glucan production, with CslF6 likely being of major importance. Here, we report a genome‐wide association study (GWAS) to identify quantitative trait loci (QTLs) controlling β‐glucan production in oat. Three panels of elite accessions (Spring, Winter, and World Diversity) of oat were grown in multiple North American locations and genotyped using the Oat 6K Custom Infinium iSelect BeadChip. Independent GWAS were performed on each set of accessions and locations, with a meta‐analysis identifying 58 significantly associated markers. Synteny with the barley (Hordeum vulgare L.) genome identified four major regions of interest revealing the CslF and CslH gene families along with UGPase and AGPase as candidate genes. Subgenome‐specific expression of the A, C, and D AsCslF6 homoeologs revealed that AsCslF6_C is the least expressed in all tissue types and time points, with low‐β‐glucan varieties recording the highest proportion of AsCslF6_C expression. Linkage mapping of the homoeologs placed AsCslF6_D on consensus linkage group Mrg02 overlapping with QTL 2.2 and AsCslF6_A on Mrg12 flanked by markers associated with QTL 12.2. Many QTLs identified in this study were homoeologous, representing different gene copies duplicated in ancestral genomes, suggesting that multiple homoeologous copies of β‐glucan biosynthesis genes are contributing to the overall phenotype.}, number={2}, journal={CROP SCIENCE}, author={Fogarty, Melissa C. and Smith, Scott M. and Sheridan, Jaime L. and Hu, Gongshe and Islamovic, Emir and Reid, Rob and Jackson, Eric W. and Maughan, Peter J. and Ames, Nancy P. and Jellen, Eric N. and et al.}, year={2020}, pages={914–933} } @article{chaffin_huang_smith_bekele_babiker_gnanesh_foresman_blanchard_jay_reid_et al._2016, title={A Consensus Map in Cultivated Hexaploid Oat Reveals Conserved Grass Synteny with Substantial Subgenome Rearrangement}, volume={9}, ISSN={1940-3372}, url={http://dx.doi.org/10.3835/plantgenome2015.10.0102}, DOI={10.3835/plantgenome2015.10.0102}, abstractNote={Hexaploid oat (Avena sativa L., 2n = 6x = 42) is a member of the Poaceae family and has a large genome (∼12.5 Gb) containing 21 chromosome pairs from three ancestral genomes. Physical rearrangements among parental genomes have hindered the development of linkage maps in this species. The objective of this work was to develop a single high‐density consensus linkage map that is representative of the majority of commonly grown oat varieties. Data from a cDNA‐derived single‐nucleotide polymorphism (SNP) array and genotyping‐by‐sequencing (GBS) were collected from the progeny of 12 biparental recombinant inbred line populations derived from 19 parents representing oat germplasm cultivated primarily in North America. Linkage groups from all mapping populations were compared to identify 21 clusters of conserved collinearity. Linkage groups within each cluster were then merged into 21 consensus chromosomes, generating a framework consensus map of 7202 markers spanning 2843 cM. An additional 9678 markers were placed on this map with a lower degree of certainty. Assignment to physical chromosomes with high confidence was made for nine chromosomes. Comparison of homeologous regions among oat chromosomes and matches to orthologous regions of rice (Oryza sativa L.) reveal that the hexaploid oat genome has been highly rearranged relative to its ancestral diploid genomes as a result of frequent translocations among chromosomes. Heterogeneous chromosome rearrangements among populations were also evident, probably accounting for the failure of some linkage groups to match the consensus. This work contributes to a further understanding of the organization and evolution of hexaploid grass genomes.}, number={2}, journal={The Plant Genome}, publisher={Crop Science Society of America}, author={Chaffin, Ashley S. and Huang, Yung-Fen and Smith, Scott and Bekele, Wubishet A. and Babiker, Ebrahiem and Gnanesh, Belaghihalli N. and Foresman, Bradley J. and Blanchard, Steven G. and Jay, Jeremy J. and Reid, Robert W. and et al.}, year={2016}, pages={0} } @article{smith_maughan_2015, title={SNP genotyping using KASPar assays}, volume={1245}, journal={Plant genotyping: methods and protocols}, author={Smith, S. M. and Maughan, P. J.}, year={2015}, pages={243–256} }