2017 journal article

Sensitivity enhancement for membrane proteins reconstituted in parallel and perpendicular oriented bicelles obtained by using repetitive cross-polarization and membrane-incorporated free radicals

JOURNAL OF BIOMOLECULAR NMR, 67(2), 135–144.

By: S. Koroloff n, D. Tesch n, E. Awosanya n & A. Nevzorov n

author keywords: Oriented-sample NMR (OS-NMR); Sensitivity enhancement; Paramagnetic relaxation enhancement; Repetitive cross-polarization; Membrane proteins; Pf1; Spectroscopic assignment; Magnetically aligned bicelles
MeSH headings : Bacteriophage Pf1; Free Radicals / chemistry; Magnetic Resonance Spectroscopy / methods; Magnetic Resonance Spectroscopy / standards; Membrane Proteins / chemistry; Signal-To-Noise Ratio; Viral Proteins / chemistry
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles

JOURNAL OF MAGNETIC RESONANCE, 256, 14–22.

By: S. Koroloff n & A. Nevzorov n

author keywords: Repetitive cross-polarization (REP-CP); Multiple CP contacts; Low RF powers; Sensitivity enhancement; Oriented samples; Membrane proteins; Solid-state NMR
MeSH headings : Algorithms; Computer Simulation; Lipid Bilayers / chemistry; Magnetic Fields; Magnetic Resonance Spectroscopy / methods; Membrane Proteins / chemistry; Membrane Proteins / radiation effects; Membrane Proteins / ultrastructure; Models, Chemical; Models, Molecular; Protein Conformation; Radio Waves; Reproducibility of Results; Sensitivity and Specificity
TL;DR: The recently developed repetitive cross-polarization (REP-CP) sequence is optimized to further increase the magnetization transfer efficiency for membrane proteins reconstituted in magnetically aligned bicelles and its performance is compared to single-contact Hartmann-Hahn cross- polarization, CP-MOIST and the adiabatic transfer. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

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