Scott Collins
Works (9)
Updated: July 13th, 2023 21:15
2022 journal article
A target expression threshold dictates invader defense and prevents autoimmunity by CRISPR-Cas13
CELL HOST & MICROBE, 30(8), 1151-+.
MeSH headings : Autoimmunity / genetics; Bacteriophages / genetics; CRISPR-Cas Systems; Plasmids; RNA
Source: Web Of Science
Added: November 21, 2022
2021 journal article
In Situ Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic Saccharomyces boulardii
ACS SYNTHETIC BIOLOGY, 10(5), 1039–1052.
author keywords: Saccharomyces boulardii; probiotic engineering; metabolic engineering; colonization profile; in situ production; beta-carotene
MeSH headings : Animals; Antineoplastic Agents / metabolism; CRISPR-Cas Systems; Feces / chemistry; Female; Gastrointestinal Microbiome; Gastrointestinal Tract / metabolism; Gastrointestinal Tract / microbiology; Gene Editing / methods; Gene Expression; Indoles / metabolism; Male; Metabolic Engineering / methods; Mice; Mice, Inbred C57BL; Microorganisms, Genetically-Modified; Multigene Family; Plasmids / genetics; Probiotics / metabolism; Promoter Regions, Genetic; Provitamins / biosynthesis; Saccharomyces boulardii / genetics; Saccharomyces boulardii / metabolism; Saccharomyces cerevisiae / genetics; beta Carotene / biosynthesis
Sources: Web Of Science, ORCID
Added: May 6, 2021
2021 journal article
Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch
NUCLEIC ACIDS RESEARCH, 49(5), 2985–2999.
MeSH headings : CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems; DNA / chemistry; Endodeoxyribonucleases / metabolism; Escherichia coli / genetics; Phosphotransferases (Alcohol Group Acceptor) / genetics; Protein Biosynthesis; RNA / chemistry; RNA / metabolism; RNA, Messenger / metabolism; Transcription, Genetic
academic.oup.com (publisher PDF)
Source: Web Of Science
Added: June 10, 2021
2020 journal article
A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9
SCIENCE ADVANCES, 6(29).
Source: Web Of Science
Added: August 10, 2020
2020 journal article
An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system
METHODS, 172, 42–50.
author keywords: Anti-CRISPR proteins; Cas9; Genome editing; sgRNA; TXTL
MeSH headings : Bacteriophages / metabolism; CRISPR-Associated Protein 9 / antagonists & inhibitors; CRISPR-Associated Protein 9 / metabolism; CRISPR-Cas Systems / genetics; Enzyme Assays / instrumentation; Enzyme Assays / methods; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / immunology; Escherichia coli / virology; Escherichia coli Proteins / antagonists & inhibitors; Escherichia coli Proteins / metabolism; Fluorescence; Gene Editing / methods; Genes, Reporter / genetics; Green Fluorescent Proteins / chemistry; Green Fluorescent Proteins / genetics; Host-Pathogen Interactions / genetics; Host-Pathogen Interactions / immunology; Protein Biosynthesis; Transcription, Genetic; Viral Proteins / metabolism
Source: Web Of Science
Added: April 6, 2020
2020 article
Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing without Promiscuous Deamination
Collins, S. P., & Beisel, C. L. (2020, September 3). MOLECULAR CELL, Vol. 79, pp. 703–704.
MeSH headings : CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Cytosine; DNA, Single-Stranded; Deamination; Gene Editing
Source: Web Of Science
Added: October 12, 2020
2019 journal article
An educational module to explore CRISPR technologies with a cell-free transcription-translation system
SYNTHETIC BIOLOGY, 4(1).
author keywords: CRISPR; Cas9; education modules; synthetic biology; TXTL
Source: Web Of Science
Added: February 27, 2020
2018 journal article
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
MOLECULAR CELL, 69(1), 146-+.
MeSH headings : CRISPR-Cas Systems / genetics; Cell-Free System / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA, Bacterial / genetics; Endonucleases / metabolism; Escherichia coli / genetics; Genetic Engineering / methods; Oryza / genetics; Protein Biosynthesis / genetics; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic / genetics
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Short DNA Containing chi Sites Enhances DNA Stability and Gene Expression in E-coli Cell-Free Transcription-Translation Systems
BIOTECHNOLOGY AND BIOENGINEERING, 114(9), 2137–2141.
author keywords: Escherichia coli; gene circuits; prototyping; RecBCD; synthetic biology; TXTL
MeSH headings : Cell-Free System / physiology; DNA, Bacterial / genetics; Escherichia coli / genetics; Exodeoxyribonuclease V / genetics; Gene Expression Regulation, Bacterial / genetics; Genetic Engineering / methods; Protein Biosynthesis / genetics; Transcription, Genetic / genetics
Source: Web Of Science
Added: August 6, 2018
