Works (9)

Updated: July 13th, 2023 21:15

2022 journal article

A target expression threshold dictates invader defense and prevents autoimmunity by CRISPR-Cas13

CELL HOST & MICROBE, 30(8), 1151-+.

By: E. Vialetto*, Y. Yu*, S. Collins n, K. Wandera*, L. Barquist* & C. Beisel n

MeSH headings : Autoimmunity / genetics; Bacteriophages / genetics; CRISPR-Cas Systems; Plasmids; RNA
Source: Web Of Science
Added: November 21, 2022

2021 journal article

In Situ Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic Saccharomyces boulardii

ACS SYNTHETIC BIOLOGY, 10(5), 1039–1052.

By: D. Durmusoglu n, I. Al'Abri n, S. Collins n, J. Cheng n, A. Eroglu n, C. Beisel n, N. Crook n

author keywords: Saccharomyces boulardii; probiotic engineering; metabolic engineering; colonization profile; in situ production; beta-carotene
MeSH headings : Animals; Antineoplastic Agents / metabolism; CRISPR-Cas Systems; Feces / chemistry; Female; Gastrointestinal Microbiome; Gastrointestinal Tract / metabolism; Gastrointestinal Tract / microbiology; Gene Editing / methods; Gene Expression; Indoles / metabolism; Male; Metabolic Engineering / methods; Mice; Mice, Inbred C57BL; Microorganisms, Genetically-Modified; Multigene Family; Plasmids / genetics; Probiotics / metabolism; Promoter Regions, Genetic; Provitamins / biosynthesis; Saccharomyces boulardii / genetics; Saccharomyces boulardii / metabolism; Saccharomyces cerevisiae / genetics; beta Carotene / biosynthesis
Sources: Web Of Science, ORCID
Added: May 6, 2021

2021 journal article

Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch

NUCLEIC ACIDS RESEARCH, 49(5), 2985–2999.

By: S. Collins n, W. Rostain*, C. Liao* & C. Beisel n

MeSH headings : CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems; DNA / chemistry; Endodeoxyribonucleases / metabolism; Escherichia coli / genetics; Phosphotransferases (Alcohol Group Acceptor) / genetics; Protein Biosynthesis; RNA / chemistry; RNA / metabolism; RNA, Messenger / metabolism; Transcription, Genetic
Source: Web Of Science
Added: June 10, 2021

2020 journal article

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9


By: D. Collias n, R. Leenay n, R. Slotkowski n, Z. Zuo*, S. Collins n, B. McGirr n, J. Liu*, C. Beisel n

Source: Web Of Science
Added: August 10, 2020

2020 journal article

An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system

METHODS, 172, 42–50.

By: K. Wandera*, S. Collins n, F. Wimmer*, R. Marshall*, V. Noireaux* & C. Beisel n

author keywords: Anti-CRISPR proteins; Cas9; Genome editing; sgRNA; TXTL
MeSH headings : Bacteriophages / metabolism; CRISPR-Associated Protein 9 / antagonists & inhibitors; CRISPR-Associated Protein 9 / metabolism; CRISPR-Cas Systems / genetics; Enzyme Assays / instrumentation; Enzyme Assays / methods; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / immunology; Escherichia coli / virology; Escherichia coli Proteins / antagonists & inhibitors; Escherichia coli Proteins / metabolism; Fluorescence; Gene Editing / methods; Genes, Reporter / genetics; Green Fluorescent Proteins / chemistry; Green Fluorescent Proteins / genetics; Host-Pathogen Interactions / genetics; Host-Pathogen Interactions / immunology; Protein Biosynthesis; Transcription, Genetic; Viral Proteins / metabolism
Source: Web Of Science
Added: April 6, 2020

2020 article

Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing without Promiscuous Deamination

Collins, S. P., & Beisel, C. L. (2020, September 3). MOLECULAR CELL, Vol. 79, pp. 703–704.

By: S. Collins n & C. Beisel n

MeSH headings : CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Cytosine; DNA, Single-Stranded; Deamination; Gene Editing
Source: Web Of Science
Added: October 12, 2020

2019 journal article

An educational module to explore CRISPR technologies with a cell-free transcription-translation system


By: D. Collias n, R. Marshall*, S. Collins n, C. Beisel* & V. Noireaux*

author keywords: CRISPR; Cas9; education modules; synthetic biology; TXTL
Source: Web Of Science
Added: February 27, 2020

2018 journal article

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

MOLECULAR CELL, 69(1), 146-+.

By: R. Marshall*, C. Maxwell n, S. Collins n, T. Jacobsen n, M. Luo n, M. Begemann*, B. Gray*, E. January* ...

MeSH headings : CRISPR-Cas Systems / genetics; Cell-Free System / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA, Bacterial / genetics; Endonucleases / metabolism; Escherichia coli / genetics; Genetic Engineering / methods; Oryza / genetics; Protein Biosynthesis / genetics; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic / genetics
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Short DNA Containing chi Sites Enhances DNA Stability and Gene Expression in E-coli Cell-Free Transcription-Translation Systems


By: R. Marshall*, C. Maxwell n, S. Collins n, C. Beisel n & V. Noireaux*

author keywords: Escherichia coli; gene circuits; prototyping; RecBCD; synthetic biology; TXTL
MeSH headings : Cell-Free System / physiology; DNA, Bacterial / genetics; Escherichia coli / genetics; Exodeoxyribonuclease V / genetics; Gene Expression Regulation, Bacterial / genetics; Genetic Engineering / methods; Protein Biosynthesis / genetics; Transcription, Genetic / genetics
Source: Web Of Science
Added: August 6, 2018