Works (11)
Updated: April 6th, 2024 05:01
2024 article
Expanding the flexibility of base editing for high-throughput genetic screens in bacteria
Gawlitt, S., Collins, S. P., Yu, Y., Blackman, S. A., Barquist, L., & Beisel, C. L. (2024, March 18). NUCLEIC ACIDS RESEARCH.
academic.oup.com (publisher PDF)
Source: Web Of Science
Added: April 1, 2024
2023 article
Interrogating two extensively self-targeting Type I CRISPR-Cas systems in Xanthomonas albilineans reveals distinct anti-CRISPR proteins that block DNA degradation
Wimmer, F., Englert, F., Wandera, K. G., Alkhnbashi, O. S., Collins, S. P., Backofen, R., & Beisel, C. L. (2023, November 28). NUCLEIC ACIDS RESEARCH.
TL;DR:
Investigation of extensive self-targeting by two CRISPR-Cas systems encoding 24 self-targeting spacers in the plant pathogen Xanthomonas albilineans shows that the native I-C and I-F1 systems are actively expressed and that CRISPR RNAs are properly processed.
(via Semantic Scholar)
Source: Web Of Science
Added: December 18, 2023
2022 journal article
A target expression threshold dictates invader defense and prevents autoimmunity by CRISPR-Cas13
CELL HOST & MICROBE, 30(8), 1151-+.
MeSH headings : Autoimmunity / genetics; Bacteriophages / genetics; CRISPR-Cas Systems; Plasmids; RNA
TL;DR:
It was found that endogenous non-essential transcripts targeted by crRNAs rarely elicited autoimmunity, and target expression levels were established as an additional criterion for immune defense by RNA-targeting CRISPR-Cas systems, preventing autoIMmunity and distinguishing pathogenic and benign invaders.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: November 21, 2022
2021 journal article
In Situ Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic Saccharomyces boulardii
ACS SYNTHETIC BIOLOGY, 10(5), 1039–1052.
author keywords: Saccharomyces boulardii; probiotic engineering; metabolic engineering; colonization profile; in situ production; beta-carotene
MeSH headings : Animals; Antineoplastic Agents / metabolism; CRISPR-Cas Systems; Feces / chemistry; Female; Gastrointestinal Microbiome; Gastrointestinal Tract / metabolism; Gastrointestinal Tract / microbiology; Gene Editing / methods; Gene Expression; Indoles / metabolism; Male; Metabolic Engineering / methods; Mice; Mice, Inbred C57BL; Microorganisms, Genetically-Modified; Multigene Family; Plasmids / genetics; Probiotics / metabolism; Promoter Regions, Genetic; Provitamins / biosynthesis; Saccharomyces boulardii / genetics; Saccharomyces boulardii / metabolism; Saccharomyces cerevisiae / genetics; beta Carotene / biosynthesis
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: May 6, 2021
2021 journal article
Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch
NUCLEIC ACIDS RESEARCH, 49(5), 2985–2999.
MeSH headings : CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems; DNA / chemistry; Endodeoxyribonucleases / metabolism; Escherichia coli / genetics; Phosphotransferases (Alcohol Group Acceptor) / genetics; Protein Biosynthesis; RNA / chemistry; RNA / metabolism; RNA, Messenger / metabolism; Transcription, Genetic
TL;DR:
The design framework thus further enables tethering of CRISPR activities to cellular states and could be designed to sense endogenous RNAs expressed only under specific growth conditions, rendering Cas12a targeting activity dependent on cellular metabolism and stress.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: June 10, 2021
2020 journal article
A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9
SCIENCE ADVANCES, 6(29).
TL;DR:
It is reported that SpyCas9 also recognizes sequences within an N(A/C/T)GG motif, which could have implications for precise editing, off-target predictions, and CRISPR-based immunity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 10, 2020
2020 journal article
An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system
METHODS, 172, 42–50.
author keywords: Anti-CRISPR proteins; Cas9; Genome editing; sgRNA; TXTL
MeSH headings : Bacteriophages / metabolism; CRISPR-Associated Protein 9 / antagonists & inhibitors; CRISPR-Associated Protein 9 / metabolism; CRISPR-Cas Systems / genetics; Enzyme Assays / instrumentation; Enzyme Assays / methods; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / immunology; Escherichia coli / virology; Escherichia coli Proteins / antagonists & inhibitors; Escherichia coli Proteins / metabolism; Fluorescence; Gene Editing / methods; Genes, Reporter / genetics; Green Fluorescent Proteins / chemistry; Green Fluorescent Proteins / genetics; Host-Pathogen Interactions / genetics; Host-Pathogen Interactions / immunology; Protein Biosynthesis; Transcription, Genetic; Viral Proteins / metabolism
TL;DR:
This work reports a rapid and scalable method for characterizing putative Acrs against Cas nucleases using an E. coli-derived cell-free transcription-translation system and demonstrates how the method can be used to measure the inhibitory activity of individual Acrs in under two days.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: April 6, 2020
2020 article
Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing without Promiscuous Deamination
Collins, S. P., & Beisel, C. L. (2020, September 3). MOLECULAR CELL, Vol. 79, pp. 703–704.
MeSH headings : CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Cytosine; DNA, Single-Stranded; Deamination; Gene Editing
TL;DR:
Jin et al. (2020) engineered new variants of CRISPR base editors that make precise genomic edits in rice protoplasts while minimizing untargeted mutagenesis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 12, 2020
2019 journal article
An educational module to explore CRISPR technologies with a cell-free transcription-translation system
SYNTHETIC BIOLOGY, 4(1).
author keywords: CRISPR; Cas9; education modules; synthetic biology; TXTL
TL;DR:
Three educational modules intended to expose undergraduate students to CRISPR technologies using TXTL are described and are expected to allow students to experience the power and promise of CRISpr technologies in the classroom and to engage with their instructor and peers about the opportunities and potential risks for society.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: February 27, 2020
2018 journal article
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
MOLECULAR CELL, 69(1), 146-+.
MeSH headings : CRISPR-Cas Systems / genetics; Cell-Free System / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA, Bacterial / genetics; Endonucleases / metabolism; Escherichia coli / genetics; Genetic Engineering / methods; Oryza / genetics; Protein Biosynthesis / genetics; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic / genetics
TL;DR:
The use of E. coli cell-free transcription-translation (TXTL) systems are presented to vastly improve the speed and scalability of CRISPR characterization and validation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Short DNA Containing chi Sites Enhances DNA Stability and Gene Expression in E-coli Cell-Free Transcription-Translation Systems
BIOTECHNOLOGY AND BIOENGINEERING, 114(9), 2137–2141.
author keywords: Escherichia coli; gene circuits; prototyping; RecBCD; synthetic biology; TXTL
MeSH headings : Cell-Free System / physiology; DNA, Bacterial / genetics; Escherichia coli / genetics; Exodeoxyribonuclease V / genetics; Gene Expression Regulation, Bacterial / genetics; Genetic Engineering / methods; Protein Biosynthesis / genetics; Transcription, Genetic / genetics
TL;DR:
It is shown that double-stranded DNA encoding χ sites-eight base-pair sequences preferentially bound by the RecBCD recombination machinery-stabilizes linear DNA and greatly enhances the TXTL-based expression and activity of a fluorescent reporter gene, simple regulatory cascades, and T7 bacteriophage particles.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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