Sofia Pinzi

Works (4)

Updated: April 5th, 2024 10:00

2023 journal article

An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera

Insects, 14(8), 683.

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n, A. Cohen n  & M. Lorenzen n 

author keywords: insect rearing; rootworm; molecular biology; function genomics; CRISPR
TL;DR: An optimized rearing system for use in western corn rootworm functional genomics research is reported, specifically the development of a system that facilitates the collection of preblastoderm embryos for microinjection as well as gathering large larvae and pupae for downstream phenotypic screening. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: September 11, 2023

2019 journal article

Effects of targeting eye color in Tenebrio molitor through RNA interference of tryptophan 2,3‐dioxygenase ( vermilion ): Implications for insect farming

Archives of Insect Biochemistry and Physiology, 101(1), e21546.

By: B. Oppert *, F. Chu n, S. Reyna n, S. Pinzi n, S. Adrianos*, L. Perkin *, M. Lorenzen n 

author keywords: agriculture; entomophagy; farming; gene expression; insect eye color; protein; RNA-Seq; Tenebrio molitor; vermilion
MeSH headings : Animal Husbandry; Animals; Eye Color / genetics; Gene Expression; Genetic Markers; Insect Proteins / genetics; Insect Proteins / metabolism; Larva / genetics; Pigmentation / genetics; Pupa / genetics; RNA Interference; Tenebrio / enzymology; Tenebrio / genetics; Tenebrio / growth & development; Tryptophan Oxygenase / genetics
TL;DR: A dominant marker system based on eye color can be developed for the genetic manipulation of T. molitor to increase the value of mealworms as an alternative food source by decreasing negative traits,such as disease susceptibility, and increasing desired traits, such as protein content and vitamin production. (via Semantic Scholar)
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2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 15, 2019

2018 journal article

Microinjection of Western Corn Rootworm, <em>Diabrotica virgifera virgifera</em>, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, (134).

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n & M. Lorenzen n 

author keywords: Bioengineering; Issue 134; embryonic microinjection; functional genomics; germline transformation; transgenesis; CRISPR/Cas9 genome editing; western corn rootworm
MeSH headings : Animals; CRISPR-Cas Systems / genetics; Coleoptera; Gene Editing / methods; Germ-Line Mutation / genetics; Microinjections / methods; RNA Interference
TL;DR: The methods used to collect and microinject precellular WCR embryos with nucleic acids are detailed, aimed at the creation of transgenic WCR, and CRISPR/Cas9-genome editing could also be performed using the same protocols, with the only difference being the composition of the solution injected into the embryos. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 16, 2018

2017 journal article

Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera

Insect Molecular Biology, 26(4), 440–452.

By: F. Chu n, W. Klobasa n, P. Wu n, S. Pinzi n, N. Grubbs n, S. Gorski  n, Y. Cardoza n, M. Lorenzen n 

author keywords: Minos; piggyBac; small-scale rearing; embryonic microinjection
MeSH headings : Animals; Coleoptera / genetics; Female; Gene Transfer Techniques; Luminescent Proteins; Male; Transposases
TL;DR: Establishing transgenic technologies for this beetle is the first step towards bringing a wide range of transformation‐based tools to bear on understanding WCR biology. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

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