@article{singh_kumar_purayannur_chen_verma_2022, title={Ascochyta rabiei: A threat to global chickpea production}, ISSN={["1364-3703"]}, DOI={10.1111/mpp.13235}, abstractNote={Abstract}, journal={MOLECULAR PLANT PATHOLOGY}, author={Singh, Ritu and Kumar, Kamal and Purayannur, Savithri and Chen, Weidong and Verma, Praveen Kumar}, year={2022}, month={Jul} }
 @misc{salcedo_purayannur_standish_miles_thiessen_quesada-ocampo_2021, title={Fantastic Downy Mildew Pathogens and How to Find Them: Advances in Detection and Diagnostics}, volume={10}, ISSN={["2223-7747"]}, url={https://doi.org/10.3390/plants10030435}, DOI={10.3390/plants10030435}, abstractNote={Downy mildews affect important crops and cause severe losses in production worldwide. Accurate identification and monitoring of these plant pathogens, especially at early stages of the disease, is fundamental in achieving effective disease control. The rapid development of molecular methods for diagnosis has provided more specific, fast, reliable, sensitive, and portable alternatives for plant pathogen detection and quantification than traditional approaches. In this review, we provide information on the use of molecular markers, serological techniques, and nucleic acid amplification technologies for downy mildew diagnosis, highlighting the benefits and disadvantages of the technologies and target selection. We emphasize the importance of incorporating information on pathogen variability in virulence and fungicide resistance for disease management and how the development and application of diagnostic assays based on standard and promising technologies, including high-throughput sequencing and genomics, are revolutionizing the development of species-specific assays suitable for in-field diagnosis. Our review provides an overview of molecular detection technologies and a practical guide for selecting the best approaches for diagnosis.}, number={3}, journal={PLANTS-BASEL}, publisher={MDPI AG}, author={Salcedo, Andres F. and Purayannur, Savithri and Standish, Jeffrey R. and Miles, Timothy and Thiessen, Lindsey and Quesada-Ocampo, Lina M.}, year={2021}, month={Mar} }
 @article{purayannur_munster_bertone_quesada-ocampo_2021, title={First Report of Downy Mildew Caused by Peronospora chenopodii-ambrosioidis on Epazote (Dysphania ambrosioides) in North Carolina}, volume={22}, ISSN={["1535-1025"]}, url={https://doi.org/10.1094/PHP-12-20-0110-FI}, DOI={10.1094/PHP-12-20-0110-FI}, abstractNote={ In this brief, we report the observation of downy mildew caused by Peronospora chenopodii-ambrosioidis on epazote (Dysphania ambrosioides) in North Carolina, U.S.A. We performed morphological characterization of the sporangia and sporangiophores for identification. We also confirmed the identity of the pathogen by performing an alignment and generating a maximum likelihood phylogeny of the concatenated internal transcribed spacer region and cytochrome c oxidase subunit I sequences. }, number={3}, journal={PLANT HEALTH PROGRESS}, publisher={Scientific Societies}, author={Purayannur, Savithri and Munster, Michael J. and Bertone, Matthew A. and Quesada-Ocampo, Lina M.}, year={2021}, pages={384–386} }
 @article{purayannur_gent_miles_radisek_quesada-ocampo_2021, title={The hop downy mildew pathogen Pseudoperonospora humuli}, volume={5}, ISSN={["1364-3703"]}, url={https://doi.org/10.1111/mpp.13063}, DOI={10.1111/mpp.13063}, abstractNote={Abstract}, journal={MOLECULAR PLANT PATHOLOGY}, publisher={Wiley}, author={Purayannur, Savithri and Gent, David H. and Miles, Timothy D. and Radisek, Sebastjan and Quesada-Ocampo, Lina M.}, year={2021}, month={May} }
 @article{purayannur_cano_bowman_childs_gent_quesada-ocampo_2020, title={The Effector Repertoire of the Hop Downy Mildew Pathogen Pseudoperonospora humuli}, volume={11}, ISSN={["1664-8021"]}, DOI={10.3389/fgene.2020.00910}, abstractNote={Pseudoperonospora humuli is an obligate biotrophic oomycete that causes downy mildew (DM), one of the most destructive diseases of cultivated hop that can lead to 100% crop loss in susceptible cultivars. We used the published genome of P. humuli to predict the secretome and effectorome and analyze the transcriptome variation among diverse isolates and during infection of hop leaves. Mining the predicted coding genes of the sequenced isolate OR502AA of P. humuli revealed a secretome of 1,250 genes. We identified 296 RXLR and RXLR-like effector-encoding genes in the secretome. Among the predicted RXLRs, there were several WY-motif-containing effectors that lacked canonical RXLR domains. Transcriptome analysis of sporangia from 12 different isolates collected from various hop cultivars revealed 754 secreted proteins and 201 RXLR effectors that showed transcript evidence across all isolates with reads per kilobase million (RPKM) values > 0. RNA-seq analysis of OR502AA-infected hop leaf samples at different time points after infection revealed highly expressed effectors that may play a relevant role in pathogenicity. Quantitative RT-PCR analysis confirmed the differential expression of selected effectors. We identified a set of P. humuli core effectors that showed transcript evidence in all tested isolates and elevated expression during infection. These effectors are ideal candidates for functional analysis and effector-assisted breeding to develop DM resistant hop cultivars.}, journal={FRONTIERS IN GENETICS}, author={Purayannur, Savithri and Cano, Liliana M. and Bowman, Megan J. and Childs, Kevin L. and Gent, David H. and Quesada-Ocampo, Lina M.}, year={2020}, month={Aug} }