Works (2)

Updated: August 26th, 2023 21:16

2019 journal article

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis


By: C. Liao n, F. Ttofali n, R. Slotkowski n, S. Denny n, T. Cecil n, R. Leenay n, A. Keung n, C. Beisel*

MeSH headings : Base Sequence; CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA / genetics; Endonucleases / metabolism; Gene Library; Genetic Techniques; HEK293 Cells; Humans; Nucleic Acid Conformation; Plasmids / metabolism; RNA / biosynthesis; RNA, Messenger / genetics; RNA, Messenger / metabolism; Saccharomyces cerevisiae / metabolism
TL;DR: A modular, one-pot scheme called CRATES to construct CRISPR arrays and array libraries that can facilitate diverse multiplexing applications and help identify factors impacting crRNA biogenesis is presented. (via Semantic Scholar)
Source: Web Of Science
Added: July 22, 2019

2016 journal article

The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers

NUCLEIC ACIDS RESEARCH, 44(15), 7385–7394.

MeSH headings : CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems / genetics; DNA / metabolism; Escherichia coli / genetics; Escherichia coli Proteins / metabolism; Gene Expression Regulation, Bacterial; Gene Silencing; Macromolecular Substances / chemistry; Macromolecular Substances / metabolism; RNA, Bacterial / chemistry; RNA, Bacterial / genetics; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic
TL;DR: It is found that extending the spacer portion of the crRNA resulted in larger Cascade complexes with altered stoichiometry and preserved in vitro binding affinity for target DNA, and spacer length can be modified to fine-tune Cascade activity. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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