@article{chang_frandsen_gadsby_2017, title={Prostaglandin synthesis by the porcine corpus luteum: effect of tumor necrosis factor-alpha}, volume={58}, ISSN={["1879-0054"]}, DOI={10.1016/j.domaniend.2016.07.001}, abstractNote={The porcine corpus luteum (CL) displays delayed sensitivity to PGF-2α (luteolytic sensitivity, [LS]) until days 12 to 13 of cycle. The control of LS is unknown, but it is temporally associated with macrophage (which secrete tumor necrosis factor-α; TNF-α) infiltration into the CL. Other studies showed that TNF-α induces LS in vitro and that prostaglandins (PGs) may be involved in this mechanism. In experiment 1, PGF-2α and PGE secretion by luteal cells (LCs) was measured on days 4 to 14 of the estrous cycle, and the expression of PTGFS/AKR1B1 and PTGES/mPGES-1, determined by Western blot, before (day 7) vs after (day 13) the onset of LS. Results showed that the PGF-2α:PGE ratio increased significantly (P < 0.05) from day 4 to 13-14, and PTGFS/AKR1B1 and PTGES/mPGES-1 were significantly increased (P < 0.05) on day 13 (vs day 7). In experiment 2, LCs were collected from porcine CL at early (∼days 4-6) or mid (∼days 7-12) stages of the estrous cycle and cultured with 0, 0.1, 1, or 10 ng/mL TNF-α. Results showed that TNF-α significantly increased (P < 0.05) messenger RNA (mRNA) expression of cyclooxygenase (COX)-2 and mPGES-1 but not AKR1B1. TNF-α had no significant effects on AKR1B1 or mPGES protein abundance. TNF-α significantly increased (P < 0.05) PGE-2 but had no effect on PGF-2α secretion or on the PGF-2α:PGE2 ratio. In conclusion, although TNF-α increased COX2 and mPGES-1 mRNA, and PGE-2 secretion in vitro, it did not increase the PGF-2α:PGE2 ratio. Studies are currently directed toward exploring other pathways (eg, FP receptor signaling) by which TNF-α induces LS in the porcine CL.}, journal={DOMESTIC ANIMAL ENDOCRINOLOGY}, author={Chang, J. and Frandsen, S. and Gadsby, J. E.}, year={2017}, month={Jan}, pages={53–62} }
 @article{medland_pohl_edwards_frandsen_bagley_li_moeser_2016, title={Early life adversity in piglets induces long-term upregulation of the enteric cholinergic nervous system and heightened, sex-specific secretomotor neuron responses}, volume={28}, ISSN={["1365-2982"]}, DOI={10.1111/nmo.12828}, abstractNote={Abstract}, number={9}, journal={NEUROGASTROENTEROLOGY AND MOTILITY}, author={Medland, J. E. and Pohl, C. S. and Edwards, L. L. and Frandsen, S. and Bagley, K. and Li, Y. and Moeser, A. J.}, year={2016}, month={Sep}, pages={1317–1329} }