Works (10)
Updated: August 16th, 2024 13:38
2010 journal article
Improving the catalytic activity of hyperthermophilic Pyrococcus prolidases for detoxification of organophosphorus nerve agents over a broad range of temperatures
Applied Microbiology and Biotechnology, 87(5), 1715–1726.
author keywords: Prolidase; Pyrococcus furiosus; OP nerve agents; Mutagenesis; Directed evolution
MeSH headings : Amino Acid Substitution / genetics; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / isolation & purification; Archaeal Proteins / metabolism; Chemical Warfare Agents / metabolism; Culture Media / chemistry; Dipeptidases / chemistry; Dipeptidases / genetics; Dipeptidases / isolation & purification; Dipeptidases / metabolism; Enzyme Stability; Escherichia coli / genetics; Models, Molecular; Mutagenesis; Organophosphorus Compounds / metabolism; Pesticides / metabolism; Plasmids; Polymerase Chain Reaction / methods; Protein Stability; Protein Structure, Tertiary; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Sequence Deletion; Temperature
TL;DR:
To obtain a better enzyme for OP nerve agent decontamination and to investigate structural factors that influence protein thermostability and thermoactivity, randomly mutated P. furiosus prolidase mutants with improved activity over a broader range of temperatures were isolated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
2009 journal article
Characterization of two proline dipeptidases (prolidases) from the hyperthermophilic archaeon Pyrococcus horikoshii
Applied Microbiology and Biotechnology, 86(1), 177–188.
author keywords: Prolidase; Pyrococcus horikoshii; Hyperthermophile; Metalloenzyme; Cobalt enzyme
MeSH headings : Amino Acid Sequence; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Biotechnology / methods; Catalytic Domain; Cobalt / metabolism; Dipeptidases / chemistry; Dipeptidases / genetics; Dipeptidases / metabolism; Enzyme Stability; Hot Temperature; Hydrogen-Ion Concentration; Molecular Sequence Data; Pyrococcus horikoshii / enzymology; Pyrococcus horikoshii / genetics; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity
TL;DR:
Biochemical characterization of two thermostable prolidases identified in the genome of Pyrococcus horikoshii shows they have higher catalytic activities over a broader pH range, higher affinity for metal and are more stable compared to P. furiosus prolidase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
2009 review
biotechnological applications of recombinant microbial prolidases
[Review of ]. Advances in applied microbiology, vol 68, 68, 99-.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
Identification of a UPC2 homolog in Saccharomyces cerevisiae and its involvement in aerobic sterol uptake
JOURNAL OF BACTERIOLOGY, 183(3), 830–834.
MeSH headings : Amino Acid Sequence; Biological Transport; Fungal Proteins / genetics; Molecular Sequence Data; Mutagenesis, Insertional; Point Mutation; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Sterols / metabolism; Trans-Activators / genetics
TL;DR:
Gas chromatographic analysis of the nonsaponifiable fractions of the various strains showed that the major sterol for all YLR228c and UPC2 combinations was ergosterol, the consensus yeast sterol.
(via Semantic Scholar)
open access via jb.asm.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2000 article
Biochemical modifications and transcriptional alterations attendant to sterol feeding in Phytophthora parasitica
Dotson, W. D., Tove, SR, & Parks, L. W. (2000, March). LIPIDS, Vol. 35, pp. 243–247.
MeSH headings : Biotransformation; Oxidoreductases / metabolism; Oxidoreductases Acting on CH-CH Group Donors; Phytophthora / genetics; Phytophthora / metabolism; Phytophthora / physiology; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Spores, Fungal / physiology; Sterols / metabolism; Substrate Specificity
TL;DR:
Characterization of sterol-related metabolic effects and sterol functions in Phytophthora should lead to improved measures for control of this important group of plant pathogens.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1999 journal article
In yeast, upc2-1 confers a decrease in tolerance to LiCl and NaCl, which can be suppressed by the P-type ATPase encoded by ENA2
DNA AND CELL BIOLOGY, 18(2), 133–139.
MeSH headings : Adenosine Triphosphatases / genetics; Cholesterol / pharmacokinetics; Dose-Response Relationship, Drug; Genes, Fungal / genetics; Genetic Complementation Test; Lithium Chloride / pharmacology; Mutation; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins; Sodium Chloride / pharmacology; Sterols / pharmacokinetics; Trans-Activators / genetics
TL;DR:
Wild-type yeast cells are unable to take up sterols from their growth media under aerobic conditions and are relatively resistant to monovalent cations, but a yeast mutant with a defect in the aerobic exclusion of sterols was found to have increased sensitivity to LiCl and NaCl.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1998 journal article
A calcium-dependent ergosterol mutant of Saccharomyces cerevisiae
CURRENT GENETICS, 34(2), 93–99.
author keywords: ergosterol; antifungal; calcium; fenpropimorph; azole; ERG24 gene
MeSH headings : Antifungal Agents / pharmacology; Calcium / metabolism; Calcium / pharmacology; Culture Media; Ergosterol / analogs & derivatives; Ergosterol / biosynthesis; Ergosterol / genetics; Ergosterol / metabolism; Genes, Fungal; Morpholines / pharmacology; Mutation; Oxidoreductases / genetics; Oxidoreductases / metabolism; Phenotype; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism
TL;DR:
A novel calcium-dependent phenotype associated with alterations in the ergosterol biosynthetic pathway in yeast is described and reduction of yeast growth with an azole inhibitor of the C-14 sterol de-methylase was also modulated by an excess of calcium ions in the culture medium.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1998 journal article
A mutation in a purported regulatory gene affects control of sterol uptake in Saccharomyces cerevisiae
Journal of Bacteriology, 180(16), 4177–4183.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
A conditional sterol esterification defect in yeast having either a SEC1 or SEC5 mutation in the secretory pathway
Yeast, 13(5), 449–462.
TL;DR:
Two temperature‐conditional secretory mutations, sec1 and sec5, cause the accumulation of post‐Golgi vesicles when strains containing these mutations are grown at 37°C, and double mutants containing sec6, sec14 or sec18 with either a sec1 or sec5 mutation have normal esterification levels.
(via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
Assessment of the essentiality of ERG genes late in ergosterol biosynthesis in Saccharomyces cerevisiae
CURRENT GENETICS, 32(2), 93–99.
author keywords: ergosterol; ERG genes; yeast; Saccharomyces
MeSH headings : 3-Isopropylmalate Dehydrogenase; Alcohol Oxidoreductases / genetics; Ergosterol / biosynthesis; Genes, Fungal; Methyltransferases / genetics; Mutation; Oxidoreductases / genetics; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / growth & development; Saccharomyces cerevisiae / metabolism; Selection, Genetic
TL;DR:
A general trend was seen in which the earlier in the biosynthetic pathway that a mutation occurred, the less able the strain producing the defective sterols could compete with the ergosterol-producing strains.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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