Shriarjun Shastry

Works (15)

Updated: January 23rd, 2026 08:05

2026 article

Adaptive Machine Learning Framework for Optimizing the Affinity Purification of Adeno‐Associated Viral Vectors

Idanwekhai, K. P., Shastry, S., Hurst, M. R., Minzoni, A., Barbieri, E., Remmler, L., … Tropsha, A. (2026, January 19). Biotechnology and Bioengineering, Vol. 1.

By: K. Idanwekhai *, S. Shastry n, M. Hurst n, A. Minzoni n, E. Barbieri*, L. Remmler*, E. Muratov, M. Daniele n , S. Menegatti n , A. Tropsha

Contributors: K. Idanwekhai *, S. Shastry n, M. Hurst n, A. Minzoni n, E. Barbieri*, L. Remmler*, E. Muratov, . al.

topics (OpenAlex): Protein purification and stability; Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: January 21, 2026

2025 article

Comparison of anion-exchange chromatography matrices for purification of linear and supercoiled plasmid in a direct lysate workflow

Stoops, M., Shastry, S., Martinez-Mata, C., Elhanafi, D., & Pancorbo, J. (2025, October 24). Journal of Chromatography A.

By: M. Stoops n, S. Shastry n, C. Martinez-Mata n, D. Elhanafi n & J. Pancorbo n

topics (OpenAlex): Protein purification and stability; Viral Infectious Diseases and Gene Expression in Insects; Monoclonal and Polyclonal Antibodies Research
Source: NC State University Libraries
Added: November 22, 2025

2025 journal article

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Bioengineering & Translational Medicine, 10(6).

By: E. Barbieri n, G. Mollica n, S. Sripada n, S. Shastry n, Y. Wu n, A. Minzoni n, W. Smith n, E. Wuestenhagen* ...

author keywords: affinity chromatography; lentivirus; membrane chromatography; peptide ligands; product polishing
topics (OpenAlex): Virus-based gene therapy research; CAR-T cell therapy research; Viral Infectious Diseases and Gene Expression in Insects
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 14, 2025

2025 article proceedings

Machine Learning-Enabled Immunosensor for Label-Free Quantification of Virus Titers

By: R. Shukla n, H. Sun n, J. Wang n, J. Twiddy n, S. Shastry n, S. Menegatti n , M. Daniele n 

Contributors: R. Shukla n, H. Sun n, J. Wang n, J. Twiddy n, S. Shastry n, S. Menegatti n , M. Daniele n 

topics (OpenAlex): Advanced Biosensing Techniques and Applications; Biosensors and Analytical Detection; Advanced biosensing and bioanalysis techniques; Virus-based gene therapy research
Sources: NC State University Libraries, NC State University Libraries, ORCID, Crossref
Added: November 19, 2025

2024 journal article

Multiangle Light Scattering as a Lentivirus Purification Process Analytical Technology

Analytical Chemistry, 96(23), 9593–9600.

By: S. Sripada n, E. Barbieri n, S. Shastry n, E. Wuestenhagen*, A. Aldinger*, O. Rammo*, M. Schulte*, M. Daniele n , S. Menegatti n 

Contributors: S. Sripada n, E. Barbieri n, S. Shastry n, E. Wuestenhagen*, A. Aldinger*, O. Rammo*, M. Schulte*, M. Daniele n , S. Menegatti n 

MeSH headings : Lentivirus / genetics; Lentivirus / isolation & purification; Humans; Dynamic Light Scattering; Chromatography, Gel; HEK293 Cells; Particle Size; Genetic Vectors / genetics; Genetic Vectors / isolation & purification
topics (OpenAlex): Spectroscopy Techniques in Biomedical and Chemical Research; Viral Infectious Diseases and Gene Expression in Insects; Protein purification and stability
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: June 11, 2024

2024 journal article

Peptide ligands for the universal purification of exosomes by affinity chromatography

Biotechnology and Bioengineering, 121(11), 3484–3501.

By: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n , W. Tian * ...

Contributors: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n , . al.

author keywords: affinity chromatography; bioseparations; exosomes; gene delivery; peptide ligands
MeSH headings : Exosomes / chemistry; Exosomes / metabolism; Chromatography, Affinity / methods; Ligands; Humans; Peptides / chemistry; Peptides / metabolism; Peptides / isolation & purification
topics (OpenAlex): Extracellular vesicles in disease; RNA Interference and Gene Delivery; Inhalation and Respiratory Drug Delivery; Lipid Membrane Structure and Behavior
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 14, 2024

2024 journal article

Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins

Journal of Chromatography A, 1734, 465320.

By: S. Shastry n, E. Barbieri n, A. Minzoni n, W. Chu n, S. Johnson n, M. Stoops n, J. Pancorbo n, G. Gilleskie n  ...

Contributors: S. Shastry n, E. Barbieri n, A. Minzoni n, W. Chu n, S. Johnson n, M. Stoops n, J. Pancorbo n, . al.

author keywords: Gene therapy; Adeno-associated virus; Peptide ligands; Affinity chromatography; Transduction activity
MeSH headings : Dependovirus / isolation & purification; Dependovirus / genetics; Dependovirus / chemistry; Chromatography, Affinity / methods; Peptides / chemistry; Peptides / isolation & purification; Serogroup; Humans; Hydrogen-Ion Concentration; Genetic Vectors; HEK293 Cells
topics (OpenAlex): Virus-based gene therapy research; RNA Interference and Gene Delivery; Viral Infectious Diseases and Gene Expression in Insects
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: September 1, 2024

2023 journal article

Development and Delivery of a Hands-On Short Course in Adeno-Associated Virus Manufacturing to Support Growing Workforce Needs in Gene Therapy

Human Gene Therapy, 34(7-8), 259–272.

By: L. Overton n, C. Boi n , S. Shastry n, C. Smith-Moore n, J. Balchunas n, D. Sambandan n, G. Gilleskie n 

author keywords: viral vectors; AAV; gene therapy; biomanufacturing; training; short course
MeSH headings : Humans; Dependovirus / genetics; Genetic Therapy; Commerce; Genetic Vectors / genetics
topics (OpenAlex): Biomedical and Engineering Education; Viral Infectious Diseases and Gene Expression in Insects; CAR-T cell therapy research
TL;DR: The design of the course, the backgrounds of the nearly 80 students who have participated in the seven offerings held since March 2019, and feedback from the course participants are discussed. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: April 19, 2023

2023 journal article

Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates

Biotechnology and Bioengineering, 120(8), 2283–2300.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback‐Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

Contributors: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, . al.

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; RNA Interference and Gene Delivery
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 7, 2023

2023 journal article

Peptide ligands targeting the vesicular stomatitis virus G (VSV‐G) protein for the affinity purification of lentivirus particles

Biotechnology and Bioengineering, 121(2), 618–639.

By: E. Barbieri n, G. Mollica n, B. Moore n, S. Sripada n, S. Shastry n, R. Kilgore n, C. Loudermilk n, Z. Whitacre n ...

author keywords: affinity chromatography; cell therapy; lentivirus; peptide ligands; vesicular stomatitis virus G protein
MeSH headings : Animals; Humans; Lentivirus / genetics; Lentivirus / metabolism; Vesicular Stomatitis; HEK293 Cells; Peptides / metabolism; Vesiculovirus / genetics; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Virology and Viral Diseases; Bacteriophages and microbial interactions
TL;DR: This study introduces peptides targeting the enveloped protein Vesicular stomatitis virus G (VSV‐G) to serve as affinity ligands for the chromatographic purification of LV particles. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 29, 2024

2023 journal article

Rational design and experimental evaluation of peptide ligands for the purification of adeno‐associated viruses via affinity chromatography

Biotechnology Journal, 19(1).

By: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback‐Clarke n, W. Smith  n, C. Cummings n, A. Minzoni n, J. Pancorbo n ...

Contributors: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback-Clarke n, W. Smith  n, C. Cummings n, A. Minzoni n, . al.

author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
MeSH headings : Humans; Dependovirus / genetics; Capsid / chemistry; HEK293 Cells; Transduction, Genetic; Peptides / metabolism; Ligands; Chromatography, Affinity; Genetic Vectors / genetics
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; CAR-T cell therapy research
TL;DR: This study introduces a cohort of peptide ligands that mimic the biorecognition activity of the AAV receptor (AAVR) and anti-AAV antibody A20, enable product elution under near-physiological conditions (pH 6.0) and grant extended reusability by withstanding multiple regenerations. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 10, 2023

2023 article

The downstream bioprocess toolbox for therapeutic viral vectors

Kilgore, R., Minzoni, A., Shastry, S., Smith, W., Barbieri, E., Wu, Y., … Menegatti, S. (2023, September 9). Journal of Chromatography A, Vol. 1709.

By: R. Kilgore n, A. Minzoni n, S. Shastry n, W. Smith n, E. Barbieri n, Y. Wu n, J. LeBarre n, W. Chu n, J. O'Brien n, S. Menegatti n 

author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Bacteriophages and microbial interactions
TL;DR: This study presents a comprehensive overview of the downstream bioprocess toolbox for viral vectors established in the last decade, and discusses present efforts and future directions contributing to the success of this promising class of biological medicines. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023

2023 article

Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy

Wang, J., Hosseini, M., Shastry, S., Barbieri, E., Chu, W., Menegatti, S., & Daniele, M. A. (2023, July 30). 2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS.

By: J. Wang n, M. Hosseini n, S. Shastry n, E. Barbieri n, W. Chu n, S. Menegatti n , M. Daniele n 

Contributors: J. Wang n, M. Hosseini n, S. Shastry n, E. Barbieri n, W. Chu n, S. Menegatti n , M. Daniele n 

author keywords: adeno-associated virus; AAV2; immunosensor; electrochemical impedance spectroscopy; viral vector
topics (OpenAlex): Virus-based gene therapy research; Viral Infections and Immunology Research; RNA Interference and Gene Delivery
TL;DR: The preliminary development of an immunosensor for measuring the titer of adeno-associated virus 2 (AAV2), which may be deployed for rapid quantification of product yield during AAV biomanufacturing. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 4, 2023

2022 article

Development of peptide ligands for the purification of α-1 antitrypsin from cell culture fluids

Chu, W., Prodromou, R., Moore, B., Elhanafi, D., Kilgore, R., Shastry, S., & Menegatti, S. (2022, July 22). Journal of Chromatography A, Vol. 1679.

By: W. Chu n, R. Prodromou n, B. Moore n, D. Elhanafi n, R. Kilgore n, S. Shastry n, S. Menegatti n 

author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
topics (OpenAlex): Protein Hydrolysis and Bioactive Peptides; Silk-based biomaterials and applications; Chemical Synthesis and Analysis
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022

2022 journal article

Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes

Membranes, 12(10), 944.

By: J. Fan n, E. Barbieri n, S. Shastry n, S. Menegatti n , C. Boi n  & R. Carbonell n

author keywords: adeno-associated virus (AAV); viral vector purification; nonwovens; membrane adsorber; anion exchange; cation exchange; membrane chromatography
topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Virus-based gene therapy research; Viral gastroenteritis research and epidemiology
TL;DR: A strong anion-exchange membrane was developed by UV grafting glycidyl methacrylate on a polybutylene terephthalate nonwoven followed by functionalization with triethylamine, resulting in a quaternary amine ligand (AEX-TEA membrane) that exhibited a high AAV2 binding capacity and achieved excellent DNA clearance and a recovery of infectivity higher that that reported using ion-ex exchange resin chromatography. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: November 14, 2022

Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.

Certain data included herein are derived from the Web of Science© and InCites© (2026) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.