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Works (11)
Updated: September 18th, 2024 05:00
2024 article
Multiangle Light Scattering as a Lentivirus Purification Process Analytical Technology
Sripada, S. A., Barbieri, E., Shastry, S., Wuestenhagen, E., Aldinger, A., Rammo, O., … Menegatti, S. (2024, May 28). ANALYTICAL CHEMISTRY, Vol. 5.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 11, 2024
2024 article
Peptide ligands for the universal purification of exosomes by affinity chromatography
Kilgore, R. E., Moore, B. D., Sripada, S. A., Chu, W., Shastry, S., Barbieri, E., … Menegatti, S. (2024, August 4). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 8.
author keywords: affinity chromatography; bioseparations; exosomes; gene delivery; peptide ligands
open access via doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 14, 2024
2024 journal article
Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins
JOURNAL OF CHROMATOGRAPHY A, 1734.
author keywords: Gene therapy; Adeno-associated virus; Peptide ligands; Affinity chromatography; Transduction activity
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 1, 2024
2023 journal article
Development and Delivery of a Hands-On Short Course in Adeno-Associated Virus Manufacturing to Support Growing Workforce Needs in Gene Therapy
Human Gene Therapy, 34(7-8), 259–272.
author keywords: viral vectors; AAV; gene therapy; biomanufacturing; training; short course
MeSH headings : Humans; Dependovirus / genetics; Genetic Therapy; Commerce; Genetic Vectors / genetics
TL;DR:
The design of the course, the backgrounds of the nearly 80 students who have participated in the seven offerings held since March 2019, and feedback from the course participants are discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
4. Quality Education
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: April 19, 2023
2023 article
Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates
Chu, W., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, July 12). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 7.
author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
TL;DR:
Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 7, 2023
2023 journal article
Peptide ligands targeting the vesicular stomatitis virus G (VSV-G) protein for the affinity purification of lentivirus particles
BIOTECHNOLOGY AND BIOENGINEERING, 121(2), 618–639.
author keywords: affinity chromatography; cell therapy; lentivirus; peptide ligands; vesicular stomatitis virus G protein
TL;DR:
This study introduces peptides targeting the enveloped protein Vesicular stomatitis virus G (VSV‐G) to serve as affinity ligands for the chromatographic purification of LV particles.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 29, 2024
2023 article
Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography
Shastry, S., Chu, W., Barbieri, E., Greback-Clarke, P., Smith, W. K., Cummings, C., … Menegatti, S. (2023, September 28). BIOTECHNOLOGY JOURNAL, Vol. 9.
author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
TL;DR:
This study introduces a cohort of peptide ligands that mimic the biorecognition activity of the AAV receptor (AAVR) and anti-AAV antibody A20, enable product elution under near-physiological conditions (pH 6.0) and grant extended reusability by withstanding multiple regenerations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 10, 2023
2023 journal article
The downstream bioprocess toolbox for therapeutic viral vectors
JOURNAL OF CHROMATOGRAPHY A, 1709.
author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
TL;DR:
This study presents a comprehensive overview of the downstream bioprocess toolbox for viral vectors established in the last decade, and discusses present efforts and future directions contributing to the success of this promising class of biological medicines.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023
2023 article
Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy
2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS.
author keywords: adeno-associated virus; AAV2; immunosensor; electrochemical impedance spectroscopy; viral vector
TL;DR:
The preliminary development of an immunosensor for measuring the titer of adeno-associated virus 2 (AAV2), which may be deployed for rapid quantification of product yield during AAV biomanufacturing.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 4, 2023
2022 journal article
Development of peptide ligands for the purification of a-1 antitrypsin from cell culture fluids
JOURNAL OF CHROMATOGRAPHY A, 1679.
author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022
2022 journal article
Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes
Membranes, 12(10), 944.
author keywords: adeno-associated virus (AAV); viral vector purification; nonwovens; membrane adsorber; anion exchange; cation exchange; membrane chromatography
TL;DR:
A strong anion-exchange membrane was developed by UV grafting glycidyl methacrylate on a polybutylene terephthalate nonwoven followed by functionalization with triethylamine, resulting in a quaternary amine ligand (AEX-TEA membrane) that exhibited a high AAV2 binding capacity and achieved excellent DNA clearance and a recovery of infectivity higher that that reported using ion-ex exchange resin chromatography.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: November 14, 2022
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