Sobhana Sripada

College of Engineering

Works (7)

Updated: June 11th, 2024 08:18

2024 journal article

Peptide ligands targeting the vesicular stomatitis virus G (VSV-G) protein for the affinity purification of lentivirus particles

BIOTECHNOLOGY AND BIOENGINEERING, 121(2), 618–639.

By: E. Barbieri n, G. Mollica n, B. Moore n, S. Sripada n, S. Shastry n, R. Kilgore n, C. Loudermilk n, Z. Whitacre n ...

author keywords: affinity chromatography; cell therapy; lentivirus; peptide ligands; vesicular stomatitis virus G protein
TL;DR: This study introduces peptides targeting the enveloped protein Vesicular stomatitis virus G (VSV‐G) to serve as affinity ligands for the chromatographic purification of LV particles. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: January 29, 2024

2023 article

Identification and characterization of CHO host-cell proteins in monoclonal antibody bioprocessing

Oh, Y. H., Mendola, K. M., Choe, L. H., Min, L., Lavoie, A. R., Sripada, S. A., … Lenhoff, A. M. (2023, October 25). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 10.

By: Y. Oh*, K. Mendola*, L. Choe*, L. Min*, A. Lavoie n, S. Sripada n, T. Williams n, K. Lee* ...

author keywords: bioprocessing; Chinese hamster ovary cells; host-cell proteins; monoclonal antibodies; purification
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 6, 2023

2023 journal article

Pseudo-affinity capture of <i>K. phaffii</i> host cell proteins in flow-through mode: Purification of protein therapeutics and proteomic study

SEPARATION AND PURIFICATION TECHNOLOGY, 326.

By: S. Sripada n, D. Elhanafi*, L. Collins n, T. Williams n, M. Linova*, J. Woodley*, C. Boi, S. Menegatti n

Contributors: S. Sripada n, D. Elhanafi*, L. Collins n, T. Williams n, M. Linova*, J. Woodley*, C. Boi, S. Menegatti n

author keywords: Komagataella phaffii; Chromatography; Protein purification; Monoclonal antibodies; Antibody fragment; Proteomics
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 23, 2023

2023 journal article

Purification of a monoclonal antibody using a novel high-capacity multimodal cation exchange nonwoven membrane

SEPARATION AND PURIFICATION TECHNOLOGY, 317.

By: J. Fan n, S. Sripada n, D. Pham*, M. Linova*, J. Woodley*, S. Menegatti n, C. Boi, R. Carbonell n

author keywords: Salt-tolerant multimodal ligand; Membrane adsorber; Membrane chromatography; Nonwoven membranes; Monoclonal antibodies (mAbs); High-risk HCPs
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 5, 2023

2022 article

Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents

Sripada, S. A., Chu, W., Williams, T. I., Teten, M. A., Mosley, B. J., Carbonell, R. G., … Menegatti, S. (2022, April 22). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 119.

By: S. Sripada n, W. Chu n, T. Williams n, M. Teten n, B. Mosley n, R. Carbonell n, A. Lenhoff*, S. Cramer* ...

Contributors: S. Sripada n, W. Chu n, T. Williams n, M. Teten n, B. Mosley n, R. Carbonell n, A. Lenhoff*, S. Cramer* ...

author keywords: CHO; flow-through chromatography; host cell proteins; monoclonal antibodies; peptide-based adsorbents
MeSH headings : Animals; Antibodies, Monoclonal / chemistry; CHO Cells; Cell Culture Techniques; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Peptides / chemistry; Proteomics / methods
TL;DR: An ensemble of peptide ligands that target the HCPs in Chinese hamster ovary (CHO) cell culture fluids and enable mAb purification via flow‐through affinity chromatography are introduced and their integration into LigaGuard™, an affinity adsorbent featuring an equilibrium binding capacity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: April 10, 2022

2021 article

Longitudinal monitoring of cell metabolism in biopharmaceutical production using label-free fluorescence lifetime imaging microscopy

Sternisha, S. M., Mukherjee, P., Alex, A., Chaney, E. J., Barkalifa, R., Wan, B., … Boppart, S. A. (2021, June 4). BIOTECHNOLOGY JOURNAL.

By: S. Sternisha*, P. Mukherjee*, A. Alex*, E. Chaney*, R. Barkalifa*, B. Wan*, J. Lee*, J. Rico-Jimenez* ...

author keywords: bioreactor; fluorescence lifetime imaging microscopy; mAb production; principal component analysis; process analytical technology
MeSH headings : Animals; Biological Products; CHO Cells; Cricetinae; Cricetulus; Microscopy, Fluorescence; NAD
TL;DR: This is the first study to use FLIM as a label‐free measure of cellular metabolism in a biopharmaceutically relevant and clinically important CHO cell line and to identify differences in metabolic progression of two cell lines cultured in bioreactors. (via Semantic Scholar)
Source: Web Of Science
Added: June 14, 2021

2021 article

Purification of polyclonal immunoglobulin G from human serum using peptide-based adsorbents

Chu, W., Sripada, S. A., Reese, H. R., Bhandari, D., Adams, A., Sly, J., … Menegatti, S. (2021, October 20). AICHE JOURNAL, Vol. 10.

By: W. Chu n, S. Sripada n, H. Reese n, D. Bhandari, A. Adams n, J. Sly, M. Crapanzano, S. Menegatti n

author keywords: affinity ligands; LigaGuard (TM); LigaTrap (TM); polyclonal antibodies; protein chromatography
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 1, 2021

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