Steven Wright Bowen

Works (5)

Updated: July 5th, 2023 15:55

2015 journal article

Transgenic and Mutation-Based Suppression of a Berberine Bridge Enzyme-Like (BBL) Gene Family Reduces Alkaloid Content in Field-Grown Tobacco

PLOS ONE, 10(2).

By: R. Lewis n , H. Lopez n, S. Bowen n, K. Andres n, W. Steede n & R. Dewey n

MeSH headings : Alkaloids / biosynthesis; Alkaloids / chemistry; Gene Expression Regulation, Plant; Mutation; Plant Proteins / antagonists & inhibitors; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; RNA Interference; Tobacco / genetics; Tobacco / growth & development; Tobacco / metabolism
topics (OpenAlex): Plant Genetic and Mutation Studies; Plant tissue culture and regeneration; GABA and Rice Research
TL;DR: Transgenic doubled haploid lines of tobacco cultivar K326 carrying an RNAi construct designed to reduce expression of the BBL gene family were produced, indicating that a range of alkaloid phenotypes could be produced, with the triple homozygous knockout genotype exhibiting greater than a 13-fold reduction in percent total alkaloids. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Development of CAPS and dCAPS markers for CYP82E4, CYP82E5v2 and CYP82E10 gene mutants reducing nicotine to nornicotine conversion in tobacco

MOLECULAR BREEDING, 29(3), 589–599.

By: D. Li *, R. Lewis n , A. Jack*, R. Dewey n, S. Bowen n & R. Miller *

author keywords: CAPS markers; dCAPS markers; Nicotiana tabacum; Nicotine conversion; Tobacco harm reduction; Mutation breeding
topics (OpenAlex): Mycotoxins in Agriculture and Food; Plant Genetic and Mutation Studies; Plant tissue culture and regeneration
TL;DR: Efficient, user-friendly DNA markers to identify mutations based on single nucleotide polymorphisms (SNPs) can be used in marker-assisted selection programs to quickly introgress the desired mutations into commercial varieties in order to reduce nornicotine levels in tobacco leaves. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Three nicotine demethylase genes mediate nornicotine biosynthesis in Nicotiana tabacum L Functional characterization of the CYP82E10 gene

PHYTOCHEMISTRY, 71(17-18), 1988–1998.

By: R. Lewis n , S. Bowen n, M. Keogh n & R. Dewey n

author keywords: Nicotiana tabacum; Solanaceae; Gene function; Alkaloids; Nornicotine; Tobacco specific nitrosamine (TSNA); Cytochrome P450; CYP82E10
MeSH headings : Carcinogens / chemistry; Carcinogens / pharmacology; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Molecular Structure; Nicotine / analogs & derivatives; Nicotine / biosynthesis; Nicotine / chemistry; Nicotine / genetics; Nicotine / metabolism; Nitrosamines / chemistry; Nitrosamines / pharmacology; Plant Leaves / chemistry; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Tobacco / chemistry; Tobacco / enzymology; Tobacco / genetics
topics (OpenAlex): Plant Genetic and Mutation Studies; Plant-Microbe Interactions and Immunity; Plant tissue culture and regeneration
TL;DR: The introduction of knockout mutations in all three tobacco NND genes into commercial breeding lines promises to be a viable strategy for reducing the levels of one of the best characterized animal carcinogens found in tobacco products. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

CYP82E4-mediated nicotine to nornicotine conversion in tobacco is regulated by a senescence-specific signaling pathway

PLANT MOLECULAR BIOLOGY, 66(4), 415–427.

By: M. Chakrabarti *, S. Bowen n, N. Coleman*, K. Meekins*, R. Dewey n & B. Siminszky*

author keywords: alkaloid; cytochrome P450; SAG; senescence-associated gene; stress; transcriptional regulation
MeSH headings : Aging / genetics; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Ethylenes / pharmacology; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Glucuronidase / genetics; Glucuronidase / metabolism; Plant Leaves / enzymology; Plant Leaves / growth & development; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / enzymology; Promoter Regions, Genetic; Signal Transduction; Tobacco / drug effects; Tobacco / enzymology; Tobacco / genetics; Tobacco / growth & development
topics (OpenAlex): Plant Gene Expression Analysis; Plant Molecular Biology Research; Plant Parasitism and Resistance
TL;DR: The results suggest that the expression of CYP 82E4 is senescence-specific in the leaves and the use of the CYP82E4 promoter could provide a valuable tool for regulating gene expression in the senescing leaves. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Functional analysis of nicotine demethylase genes reveals insights into the evolution of modern tobacco

JOURNAL OF BIOLOGICAL CHEMISTRY, 282(1), 249–256.

By: L. Gavilano*, N. Coleman*, S. Bowen n & B. Siminszky*

MeSH headings : Alleles; Amino Acid Sequence; Cytochrome P-450 Enzyme System / biosynthesis; Cytochrome P-450 Enzyme System / chemistry; Kinetics; Models, Biological; Models, Genetic; Molecular Sequence Data; Nicotine; Oxidoreductases / genetics; Oxidoreductases / metabolism; Phenotype; Plant Proteins / biosynthesis; Plant Proteins / chemistry; Protein Structure, Tertiary; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Stem Cells / metabolism; Tobacco / enzymology; Tobacco / genetics; Transcription, Genetic
topics (OpenAlex): Plant Gene Expression Analysis; Plant-Microbe Interactions and Immunity; Plant tissue culture and regeneration
TL;DR: Dom swapping and site-directed mutagenesis studies identified a single amino acid change that fully restored nicotine N-demethylase activity to CYP82E3, suggesting that a single base pair mutation in CYP72E3 and transcriptional suppression of CYP 82E4 played important roles in the evolution of the alkaloid profile characteristic of modern tobacco. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

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