@article{wu_jiang_jiang_wu_ding_jin_2021, title={Impacts of cotton linter pulp characteristics on the processivity of glycoside hydrolase family 5 endoglucanase from Volvariella Volvacea}, volume={28}, ISSN={["1572-882X"]}, DOI={10.1007/s10570-020-03665-x}, number={4}, journal={CELLULOSE}, author={Wu, Shanshan and Jiang, Xiao and Jiang, Huicong and Wu, Shufang and Ding, Shaojun and Jin, Yongcan}, year={2021}, month={Mar}, pages={1947–1959} } @article{zhang_jiang_wan_wu_wu_jin_2020, title={Adsorption behavior of two glucanases on three lignins and the effect by adding sulfonated lignin}, volume={323}, ISSN={["1873-4863"]}, DOI={10.1016/j.jbiotec.2020.07.013}, abstractNote={The adsorption behaviors of two glucanases, TvEG and TrCel7A, on three lignins were investigated. Three lignins were isolated from raw aspen and its pretreated solid residue. The isolated lignins were labeled as Asp-MWL, DA-MWL (pretreated by dilute acid), and GL-MWL (pretreated by green liquor), respectively. The surface properties of lignins and spin-coated lignin films were characterized by zeta potential, atomic force microscope (AFM) and contact angle. The enzyme adsorption behavior was monitored by quartz crystal microbalance (QCM) and fluorescence spectrometer. TlCel7A had similar adsorption capacities on the three lignin films but were higher than those of TvEG. The TrCel7A adsorptions on the three lignin films were affected by synergistic effect of electrostatic and hydrophobic interaction while the TvEG adsorptions on the three lignin films were mainly dominated by hydrophobic action. The adsorption capacities of TlCel7A and TvEG on the three lignin films were decreased by adding SL. Plausible explanation was that the SL and glucanase formed a complex with more negative charges, which suppressed the adsorption of glucannase on lignin through electrostatic repulsion. It also explained the improved enzymatic hydrolysis efficiency of lignocellulose upon adding SL.}, journal={JOURNAL OF BIOTECHNOLOGY}, author={Zhang, Yuqing and Jiang, Xiao and Wan, Shanqi and Wu, Wenjuan and Wu, Shufang and Jin, Yongcan}, year={2020}, month={Nov}, pages={1–8} } @article{wu_zhang_jiang_wang_liu_wu_2020, title={Changes in supramolecular structure and improvement in reactivity of dissolving pulp via enzymatic pretreatment with processive endoglucanase EG1 from Volvaria volvacea}, volume={40}, ISSN={["1532-2319"]}, DOI={10.1080/02773813.2020.1722700}, abstractNote={Processive endoglucanase EG1 and its core domain, EG1(CD), were used to pretreat the commercial dissolving pulp to improve cellulose reactivity. The Fock reactivity of the pulp which was treated wi...}, number={3}, journal={JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY}, author={Wu, Shanshan and Zhang, Yuemei and Jiang, Xiao and Wang, Shulei and Liu, Jiang and Wu, Shufang}, year={2020}, month={May}, pages={163–171} } @article{wu_chen_jameel_chang_phillips_jin_2020, title={Effects of Lignin Contents and Delignification Methods on Enzymatic Saccharification of Loblolly Pine}, volume={59}, ISSN={["0888-5885"]}, DOI={10.1021/acs.iecr.0c00645}, abstractNote={Softwood pulp is more recalcitrant toward enzymatic hydrolysis than hardwood or nonwood pulps. Pulps of various kappa numbers were prepared from loblolly pine chips by kraft cooking, which was followed by delignifying with oxygen/NaOH, chlorine dioxide, or ozone to lower lignin levels. These pulps were subject to enzymatic saccharification to investigate the effects of lignin content and delignification methods. Kappa number is not a good indication of lignin content of chlorine dioxide- and oxygen-delignified pulps. Both the lignin content of the pulp and the method of delignification affect enzymatic saccharification. Decreasing lignin content of a given pulp improves saccharification efficiency. The delignification methods have a profound influence on enzymatic hydrolysis efficiency, with chlorine dioxide- and oxygen-delignified pulps being more efficient than ozone-delignified and kraft pulps. X-ray photoelectric spectroscopy analysis shows that the surface lignin content of the pulp may account for the difference between the three methods of oxidative delignification.}, number={18}, journal={INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH}, author={Wu, Shufang and Chen, Hui and Jameel, Hasan and Chang, Hou-min and Phillips, Richard and Jin, Yongcan}, year={2020}, month={May}, pages={8532–8537} } @article{wu_jameel_chang_phillips_2014, title={Techno-economic analysis of the optimum softwood lignin content for the production of bioethanol in a repurposed kraft mill}, volume={9}, DOI={10.15376/biores.9.4.6817-6830}, abstractNote={Kraft pulping is one possible pretreatment for softwood to economically produce bioethanol. This work evaluates the techno-economic potential of using the kraft process for producing bioethanol from softwoods in a repurposed or co-located kraft mill. Pretreated loblolly pine was enzymatically hydrolyzed at low enzyme dosages of 5 and 10 FPU/g of substrate. Pretreated residue with 13% lignin content had the highest sugar recovery, 32.7% and 47.7% at 5 and 10 FPU/g, respectively. The pretreated residues were oxygen delignified and refined. In all cases, oxygen delignification improved sugar recovery, while refining was mostly effective for pulps with high lignin content. At 5 FPU/g, the sugar recovery for all kraft pulps was 51 to 53% with oxygen delignification and refining. Increasing the enzyme dosage to 10 FPU/g increased the sugar recovery for these pulps to greater than 60%. Economic analysis for the pulps with different initial lignin content showed that kraft pulps with an initial lignin content of 6.7% with oxygen delignification had an ethanol yield of 285 L/ODt wood and the lowest total production cost of $0.55/L. Pulps with initial lignin content of 18.6% had a total production cost of $0.64/L with an ethanol yield of 264 L/ODt wood.}, number={4}, journal={BioResources}, author={Wu, S. F. and Jameel, H. and Chang, H. M. and Phillips, R.}, year={2014}, pages={6817–6830} } @article{wu_chang_jameel_philips_2012, title={Effect of Additives on Polysaccharide Retention in Green Liquor Pretreatment of Loblolly Pine for Enzymatic Hydrolysis}, volume={32}, ISSN={["1532-2319"]}, DOI={10.1080/02773813.2012.678959}, abstractNote={Abstract In order to improve polysaccharide retention during pretreatment and increase enzymatic hydrolysis efficiency, green liquor (GL) pretreatment was performed at: (1) higher GL sulfidity; (2) higher sulfidity with the addition of sodium polysulfide; (3) higher sulfidity with the addition of sodium borohydride; or (4) higher sulfudity with the addition of sodium tetraborate. The results indicate that green liquor pretreatment with the higher sulfidity, with the addition of polysulfide and with the addition of sodium borohydride, is effective in increasing enzymatic conversion of the pretreated pulps. The addition of sodium tetraborate, on the other hand, is not effective. These measures can be combined with oxygen delignification and/or mechanical refining to further improve the enzymatic hydrolysis efficiency. Up to 80% of the polysaccharides in softwood can be converted to fermentable sugars, but only with the addition of 40 FPU enzyme dose.}, number={4}, journal={JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY}, author={Wu, Shu-Fang and Chang, Hou-Min and Jameel, Hasan and Philips, Richard}, year={2012}, pages={317–327} }