@article{dantas araujo_xavier-santos_silva_lima_schlamb_fernandes-pedrosa_silva junior_araujo junior_rathinasabapathy_moncada_et al._2023, title={Gel formulated with Bryophyllum pinnatum leaf extract promotes skin wound healing in vivo by increasing VEGF expression: A novel potential active ingredient for pharmaceuticals}, volume={13}, ISSN={["1663-9812"]}, DOI={10.3389/fphar.2022.1104705}, abstractNote={Bryophyllumpinnatum(Crassulaceae) is used in traditional medicine for treating skin wounds. In our previous study, a topical gel containingB. pinnatumaqueous leaf extract showed a preclinical anti-inflammatory effect inin vivoacute edema models. In continuation, the present study aims to evaluate the phytochemical content and the stability of a formulation in gel containingB. pinnatumaqueous leaf extract and its healing properties and mechanism of action through an experimental model of induction of skin wounds in rats andin vitroassays. The animals were treated topically for 7 or 14 days with a formulation in gel containing extract at 5% or a placebo or Fibrinase®in cream. In addition, to establish some quality control parameters, the total phenolic content (TPC), total flavonoid content (TFC), and a study focusing on the phytochemical and biological stability of a gel for 30 days at two different conditions (room temperature and 40°C/75% RH) were performed. Gel formulation containing extract showed a TPC and TFC of 2.77 ± 0.06 mg of gallic acid/g and 1.58 ± 0.03 mg of quercetin/g, respectively. Regarding the stability study, the formulation in gel showed no significant change in the following parameters: pH, water activity, chromatographic profile, and the content of the major compound identified in the extract. The gel formulation containing extract stimulated skin wound healing while reducing the wound area, as well as decreasing the inflammatory infiltrate, reducing the levels of IL-1β and TNF-α, and stimulating angiogenesis with increased expression of VEGF, an effect similar to Fibrinase. In conclusion, the gel formulation containing extract exhibited relevant skin wound healing properties and, therefore, has the potential to be applied as a novel active ingredient for developing wound healing pharmaceuticals.}, journal={FRONTIERS IN PHARMACOLOGY}, author={Dantas Araujo, Edilane Rodrigues and Xavier-Santos, Jacinthia Beatriz and Silva, Valeria Costa and Lima, Juliana Bessa and Schlamb, Jade and Fernandes-Pedrosa, Matheus de Freitas and Silva Junior, Arnobio Antonio and Araujo Junior, Raimundo Fernandes and Rathinasabapathy, Thirumurugan and Moncada, Marvin and et al.}, year={2023}, month={Jan} }
 @article{silva_guerra_araujo_schlamb_silva_tavares_dantas-medeiros_abreu_tavares_araujo junior_et al._2023, title={Phenolic-rich extract of Nopalea cochenillifera attenuates gastric lesions induced in experimental models through inhibiting oxidative stress, modulating inflammatory markers and a cytoprotective effect}, volume={2}, ISSN={["2042-650X"]}, DOI={10.1039/d2fo03735a}, abstractNote={
            Nopalea cochenillifera (Cactaceae), popularly known as “palma” or “palma doce”, is from Mexico, but it was widely introduced in Brazil through crops.}, journal={FOOD & FUNCTION}, author={Silva, Elaine Cristine Souza and Guerra, Gerlane Coelho Bernardo and Araujo, Edilane Rodrigues Dantas and Schlamb, Jade and Silva, Valeria Costa and Tavares, Emanuella de Aragao and Dantas-Medeiros, Renato and Abreu, Lucas Silva and Tavares, Josean Fechine and Araujo Junior, Raimundo Fernandes and et al.}, year={2023}, month={Feb} }
 @article{pereira silva_cavalcanti inacio_silva_silva_luz_almeida_moraes_esposito_ferreira_zucolotto_2022, title={The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil}, volume={11}, ISSN={["2304-8158"]}, url={https://www.mdpi.com/2304-8158/11/10/1452}, DOI={10.3390/foods11101452}, abstractNote={Flavonoids are significant antioxidant and anti-inflammatory agents and have multiple potential health applications. Moringa oleifera is globally recognized for its nutritional and pharmacological properties, correlated to the high flavonoid content in its leaves. However, the bioactive compounds found in plants may vary according to the cultivation, origin, season, and extraction process used, making it difficult to extract reliable raw material. Hence, this study aimed to standardize the best cultivation and harvest season in Brazil and the best extraction process conditions to obtain a flavonoid-rich extract from M. oleifera as a final product. Firstly, ultrasound-assisted extraction (UAE) was optimized to reach the highest flavonoid content by three-level factorial planning and response surface methodology (RSM). The optimal cultivation condition was mineral soil fertilizer in the drought season, and the optimized extraction was with 80% ethanol and 13.4 min of extraction time. The flavonoid-rich extract was safe and significantly decreased reactive oxygen species (ROS) and nitric oxide (NO) in LPS-treated RAW 264.7 cells. Lastly, the major flavonoids characterized by HPLC-ESI-QTRAP-MS/MS were compounds derived from apigenin, quercetin, and kaempferol glycosides. The results confirmed that it was possible to standardize the flavonoid-rich extract leading to a standardized and reliable raw material extracted from M. oleifera leaves.}, number={10}, journal={FOODS}, author={Pereira Silva, Larissa Marina and Cavalcanti Inacio, Maria Raquel and Silva, Gualter Guenter and Silva, Jucier Magson and Luz, Jefferson Romaryo and Almeida, Maria das Gracas and Moraes, Edgar Perin and Esposito, Debora and Ferreira, Leandro De Santis and Zucolotto, Silvana Maria}, year={2022}, month={May} }