@article{hancock_tallury_isleib_chu_ozias-akins_stalker_2019, title={Introgression Analysis and Morphological Characterization of an Arachis hypogaea x A. diogoi Interspecific Hybrid Derived Population}, volume={59}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2018.07.0461}, abstractNote={Cultivated peanut (Arachis hypogaea L.) is an economically important crop grown around the world. Compared with the entire Arachis genus, cultivated peanut germplasm has low levels of genetic diversity for several economically important traits, resulting in the need for alternative sources of favorable alleles. Wild diploid species of Arachis are a source of such alleles to improve cultivated peanut for many economically important traits. An A. hypogaea × A. diogoi Hoehne introgression population was produced via the triploid–hexaploid method; the fourth generation after tetraploidy was used to initiate this study. The introgression lines were genotyped using a single nucleotide polymorphism (SNP) marker array to estimate the percentage of A. diogoi chromatin introgression. Morphologically, the introgression lines varied for an array of measured traits, with the majority being intermediate to the two parents. The average amount of A. diogoi genome introgressed was 8.12% across the tetraploid genome and ranged from 3.00 to 18.14% on individual chromosomes. The average A. diogoi introgression across all lines was 7.70% and ranged from 0.17 to 51.12%. Principal component analysis of morphological data and SNP markers revealed similarities and groupings of introgression lines. This introgression population demonstrates the potential of using wild diploid Arachis species for peanut improvement and has great potential for use in cultivated peanut breeding programs.}, number={2}, journal={CROP SCIENCE}, author={Hancock, Wesley G. and Tallury, Shyam P. and Isleib, Thomas G. and Chu, Ye and Ozias-Akins, Peggy and Stalker, H. Thomas}, year={2019}, pages={640–649} }
 @article{rosas-anderson_sinclair_balota_tallury_isleib_rufty_2014, title={Genetic Variation for Epidermal Conductance in Peanut}, volume={54}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2013.07.0461}, abstractNote={ABSTRACT}, number={2}, journal={CROP SCIENCE}, author={Rosas-Anderson, Pablo and Sinclair, Thomas R. and Balota, Maria and Tallury, Shyam and Isleib, Thomas G. and Rufty, Thomas}, year={2014}, pages={730–737} }
 @article{tallury_isleib_copeland_rosas-anderson_balota_singh_stalker_2014, title={Registration of Two Multiple Disease-Resistant Peanut Germplasm Lines Derived from Arachis cardenasii Krapov. & WC Gregory, GKP 10017}, volume={8}, ISSN={["1940-3496"]}, DOI={10.3198/jpr2013.04.0017crg}, abstractNote={Two tetraploid (2n = 4x = 40) peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) germplasm lines, GP-NC WS 16 (SPT 06-06) (Reg. No. GP-235, PI 669445) and GP-NC WS 17 (SPT 06-07) (Reg. No. GP-236, PI 669446), derived from interspecific hybridization, were developed in the peanut genetics program at North Carolina State University (NCSU), Raleigh, NC. These two lines were tested extensively by the North Carolina Agricultural Research Service from 2006 through 2012 in disease evaluation tests. They have unique alleles introgressed from the diploid (2n = 2x = 20) wild species, A. cardenasii Krapov. & W.C. Gregory. The germplasm lines are also unique in that they exhibited multiple disease resistances superior to the germplasm lines derived from A. cardenasii that were released previously by NCSU. Resistance to multiple diseases included early leaf spot (ELS), Cylindrocladium black rot (CBR), Sclerotinia blight (SB), and tomato spotted wilt (TSW). One of the lines, GP-NC WS 17, also exhibited drought tolerance in field and greenhouse studies. Thus, it can be concluded that these two peanut germplasm lines derived from diploid wild species have multiple biotic stress resistances, specifically for ELS, CBR, SB, and TSWV, as well as abiotic stress resistance in the case of GP-NC WS 17. These two lines should provide unique, improved germplasm for breeders interested in multiple disease resistance and in expanding the germplasm pool of A. hypogaea.}, number={1}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Tallury, S. P. and Isleib, T. G. and Copeland, S. C. and Rosas-Anderson, P. and Balota, M. and Singh, D. and Stalker, H. T.}, year={2014}, month={Jan}, pages={86–89} }
 @article{livingston_henson_tuong_wise_tallury_duke_2013, title={Histological Analysis and 3D Reconstruction of Winter Cereal Crowns Recovering from Freezing: A Unique Response in Oat (Avena sativa L.)}, volume={8}, ISSN={["1932-6203"]}, DOI={10.1371/journal.pone.0053468}, abstractNote={The crown is the below ground portion of the stem of a grass which contains meristematic cells that give rise to new shoots and roots following winter. To better understand mechanisms of survival from freezing, a histological analysis was performed on rye, wheat, barley and oat plants that had been frozen, thawed and allowed to resume growth under controlled conditions. Extensive tissue disruption and abnormal cell structure was noticed in the center of the crown of all 4 species with relatively normal cells on the outside edge of the crown. A unique visual response was found in oat in the shape of a ring of cells that stained red with Safranin. A tetrazolium analysis indicated that tissues immediately inside this ring were dead and those outside were alive. Fluorescence microscopy revealed that the barrier fluoresced with excitation between 405 and 445 nm. Three dimensional reconstruction of a cross sectional series of images indicated that the red staining cells took on a somewhat spherical shape with regions of no staining where roots entered the crown. Characterizing changes in plants recovering from freezing will help determine the genetic basis for mechanisms involved in this important aspect of winter hardiness.}, number={1}, journal={PLOS ONE}, author={Livingston, David P., III and Henson, Cynthia A. and Tuong, Tan D. and Wise, Mitchell L. and Tallury, Shyamalrau P. and Duke, Stanley H.}, year={2013}, month={Jan} }
 @article{friend_quandt_tallury_stalker_hilu_2010, title={Species, genomes, and section relationships in the genus Arachis (Fabaceae): a molecular phylogeny}, volume={290}, ISSN={["1615-6110"]}, DOI={10.1007/s00606-010-0360-8}, number={1-4}, journal={PLANT SYSTEMATICS AND EVOLUTION}, author={Friend, S. A. and Quandt, D. and Tallury, S. P. and Stalker, H. T. and Hilu, K. W.}, year={2010}, month={Dec}, pages={185–199} }
 @article{livingston_tallury_2009, title={Freezing in non-acclimated oats. II: Thermal response and histology of recovery in gradual and rapidly frozen plants}, volume={481}, ISSN={["1872-762X"]}, DOI={10.1016/j.tca.2008.09.024}, abstractNote={Freezing in winter cereals is a complex phenomenon that can affect various plant tissues differently. To better understand how freezing affects specific tissue in the over wintering organ (crown) of winter cereal crops, non-acclimated oats (Avena sativa L.) were gradually frozen to −3 °C and tissue damage during recovery was compared to plants that had been supercooled to −3 °C and then frozen suddenly. Percentage of total water frozen, was the same whether crowns were frozen suddenly or gradually although the rate of freezing was considerably different. For example, all available water froze within 3 h in suddenly frozen crowns but it took more than 15 h for all available water to freeze in gradually frozen crowns. When plants were suddenly frozen, cells in the apical meristem were disrupted and apparently killed. In these plants re-growth was limited or non-existent. In contrast, the apical region of plants that were slowly frozen appeared undamaged but extensive vessel plugging was observed in cells of the lower crown, possibly from accumulation of phenolics or from microbial proliferation. These histological observations along with the calorimetric analysis suggested that the apical region was killed by intracellular freezing when frozen suddenly while the crown core was damaged by a process, which either induced production of putative phenolic compounds by the plant and/or permitted what appeared to be microbial proliferation in metaxylem vessels.}, number={1-2}, journal={THERMOCHIMICA ACTA}, author={Livingston, David P., III and Tallury, Shyamalrau P.}, year={2009}, month={Jan}, pages={20–27} }
 @article{livingston_tuong_haigler_avci_tallury_2009, title={Rapid Microwave Processing of Winter Cereals for Histology Allows Identification of Separate Zones of Freezing Injury in the Crown}, volume={49}, ISSN={0011-183X}, url={http://dx.doi.org/10.2135/cropsci2009.02.0077}, DOI={10.2135/cropsci2009.02.0077}, abstractNote={ABSTRACT}, number={5}, journal={Crop Science}, publisher={Wiley}, author={Livingston, D. P., III and Tuong, T. D. and Haigler, C. H. and Avci, U. and Tallury, S. P.}, year={2009}, month={Sep}, pages={1837–1842} }
 @article{barb_werner_tallury_2008, title={Cytogenetic characterization and nuclear DNA content of diploid and tetraploid forms of stokes aster}, volume={43}, number={7}, journal={HortScience}, author={Barb, J. G. and Werner, D. J. and Tallury, S. P.}, year={2008}, pages={2005–2012} }
 @article{contreras_ranney_tallury_2007, title={Reproductive behavior of diploid and allotetraploid Rhododendron L. 'fragrant affinity'}, volume={42}, number={1}, journal={HortScience}, author={Contreras, R. N. and Ranney, T. G. and Tallury, S. P.}, year={2007}, pages={31–34} }
 @article{livingston_van_premakumar_tallury_herman_2007, title={Using Arabidopsis thaliana as a model to study subzero acclimation in small grains}, volume={54}, ISSN={["1090-2392"]}, DOI={10.1016/j.cryobiol.2006.12.004}, abstractNote={The suitability of using Arabidopsis as a model plant to investigate freezing tolerance was evaluated by observing similarities to winter cereals in tissue damage following controlled freezing and determining the extent to which Arabidopsis undergoes subzero-acclimation. Plants were grown and frozen under controlled conditions and percent survival was evaluated by observing re-growth after freezing. Paraffin embedded sections of plants were triple stained and observed under light microscopy. Histological observations of plants taken 1 week after freezing showed damage analogous to winter cereals in the vascular tissue of roots and leaf axels but no damage to meristematic regions. The LT50 of non-acclimated Arabidopsis decreased from about −6 °C to a minimum of about −13 °C after 7 days of cold-acclimation at 3 °C. After exposing cold-acclimated plants to −3 °C for 3 days (subzero-acclimation) the LT50 was lowered an additional 3°C. Defining the underlying mechanisms of subzero-acclimation in Arabidopsis may provide an experimental platform to help understand winter hardiness in economically important crop species. However, distinctive histological differences in crown anatomy between Arabidopsis and winter cereals must be taken into account to avoid misleading conclusions on the nature of winter hardiness in winter cereals.}, number={2}, journal={CRYOBIOLOGY}, author={Livingston, David P., III and Van, Kyujung and Premakumar, Ramaswamy and Tallury, Shyamalrau P. and Herman, Eliot M.}, year={2007}, month={Apr}, pages={154–163} }
 @article{livingston_premakumar_tallury_2006, title={Carbohydrate partitioning between upper and lower regions of the crown in oat and rye during cold acclimation and freezing}, volume={52}, ISSN={["1090-2392"]}, DOI={10.1016/j.cryobiol.2005.11.001}, abstractNote={Carbohydrates have long been recognized as an important aspect of freezing tolerance in plants but the association between these two factors is often ambiguous. To help clarify the relationship, the allocation of carbohydrates between specific tissues within the over wintering organ (crown) of winter cereals was measured. A winter-hardy and non-winter-hardy oat (Avena sativa L.), and a rye (Secale cereale L.) cultivar were grown and frozen under controlled conditions. Crown tissue was fractionated into an upper portion, called the apical region, and a lower portion, called the lower crown. These tissues were ground in liquid N and extracted with water. Extracts were analyzed by HPLC for the simple sugars, sucrose, glucose, fructose, and for fructan of various size classes. After 3 weeks of cold acclimation at 3 degrees C, carbohydrates accounted for approximately 40% of the dry weight of oats and 60% of the dry weight of rye. The apical region, which is the tissue within the crown that acclimates to the greatest extent, was generally 10% higher in total carbohydrates than the lower crown. During a mild freeze, various carbohydrates were allocated differently between specific tissues in the three genotypes. When frozen, fructan generally decreased to a greater extent in the lower crown than in the apical region but sugars increased more in the apical region than in the lower crown. Results suggest that to understand how carbohydrates relate to freezing tolerance, regions of the crown that endure freezing stress differently should be compared.}, number={2}, journal={CRYOBIOLOGY}, author={Livingston, DP and Premakumar, R and Tallury, SP}, year={2006}, month={Apr}, pages={200–208} }
 @article{livingston_tallury_owens_livingston_premkumar_2006, title={Freezing in nonacclimated oat: thermal response and histological observations of crowns during recovery}, volume={84}, ISSN={["0008-4026"]}, DOI={10.1139/B05-147}, abstractNote={ The complex nature of freezing in plants may be easier to understand if freezing is studied in nonacclimated plants at temperatures just below freezing. Thermal patterns of model systems frozen at –2.6 °C were compared with those of crown tissue from oat ( Avena sativa L.). Thermal patterns of live crowns more closely resembled those of fructan and sugar solutions with filter paper than of plain water or a BSA solution. When the percentage of water freezing in nonacclimated plants at –2.6 °C was manually limited to 10%, the survival was reduced from 100% in supercooled plants to 25%. During cold acclimation, the percentage of water freezing at –2.6 °C went from 79% to 54% after 3 weeks of cold acclimation and resulted in 100% survival. The nucleus of cells in the primary apical meristem of nonacclimated plants appeared to have disintegrated, an effect that was not observed in any cold-acclimated (unfrozen controls) plants. Nuclear pycnosis was observed in leaf sheaths surrounding the meristem and in cells directly below the meristem. Cells of secondary meristems and in the crown core appeared undamaged, but vessels in plants frozen for as little as 30 min were ruptured and appeared plugged. The distinctive nature of injury in the apical meristem and the rapid ability of the plant to acclimate during cold to the stress causing this injury indicate that specific tissue, namely the apical region of the crown, should be the focus of attention when attempting to determine cause and effect between genetics or metabolism and cold acclimation in winter cereals. }, number={2}, journal={CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE}, author={Livingston, DP and Tallury, SP and Owens, SA and Livingston, JD and Premkumar, R}, year={2006}, month={Feb}, pages={199–210} }
 @article{garcia_tallury_stalker_kochert_2006, title={Molecular analysis of Arachis interspecific hybrids}, volume={112}, ISSN={["1432-2242"]}, DOI={10.1007/s00122-006-0236-z}, abstractNote={Incorporation of genetic resistance against several biotic stresses that plague cultivated peanut, Arachis hypogaea (2n = 4x = 40), is an ideal option to develop disease resistant and ecologically safe peanut varieties. The primary gene pool of peanut contains many diploid wild species (2n = 2x = 20) of Arachis, which have high levels of disease and insect resistances. However, transfer of resistant genes from these species into A. hypogaea is difficult due to ploidy level differences and genomic incompatibilities. This study was conducted to monitor alien germplasm transmission, using Random Amplified Polymorphic DNA (RAPD) markers, from two diploid wild species, A. cardenasii and A. batizocoi, into A. hypogaea. Triploid interspecific hybrids were produced by crossing two A. hypogaea cultivars (NC 6 and Argentine) with the two species and by colchicine-treating vegetative meristems, fertility was restored at the hexaploid (C(o)) level in the four hybrids. Hexaploids were allowed to self-pollinate for four generations, each referred to as a cycle (C1, C2, C3, and C4). At each cycle, a backcross was made with the respective A. hypogaea cultivar as the maternal parent and only lineages tracing back to a single hexaploid hybrid were used for RAPD analysis. Analysis of mapped, species-specific RAPD markers in BC1F1 to BC1F3 hybrids indicated that alien germplasm retention decreased every generation of inbreeding, especially in Argentine and in A. batizocoi crosses. A similar trend was also observed for every cycle in BC1F2 and BC1F3 families, possibly, due to the loss of alien chromosomes following selfing of hexaploids. RAPD marker analysis of 40-chromosome interspecific hybrid derivatives from the four crosses supported previous reports that reciprocal recombination and/or translocations are the predominant mechanisms for exchange of chromosomal segments. No evidence was found for preferential transfer of alien chromosomal regions to specific linkage groups. The implications for developing disease resistant peanut breeding lines are discussed in light of these findings.}, number={7}, journal={THEORETICAL AND APPLIED GENETICS}, author={Garcia, GM and Tallury, SP and Stalker, HT and Kochert, G}, year={2006}, month={May}, pages={1342–1348} }
 @article{livingston_premakumar_tallury_2005, title={Carbohydrate concentrations in crown fractions from winter oat during hardening at sub-zero temperatures}, volume={96}, ISSN={["1095-8290"]}, DOI={10.1093/aob/mci167}, abstractNote={BACKGROUND AND AIMS
Contradictory results in correlation studies of plant carbohydrates with freezing tolerance may be because whole crown tissue is analysed for carbohydrates while differences exist in the survival of specific tissue within the crown. The aim of this study was to see if carbohydrate changes in tissue within oat crowns during second phase hardening (sub-zero hardening) are tissue specific.


METHODS
The lower portion of oat (Avena sativa) crowns was exposed to mild grinding in a blender and the remaining crown meristem complex, consisting of tough root-like vessels, was ground in a device developed specifically for grinding cereal crown tissue. Carbohydrates were extracted by water and measured by HPLC. Carbohydrate concentrations were compared in the two regions of the crown before and after hardening at sub-zero temperatures.


KEY RESULTS
Fructan of all size classes except DP>6 decreased during sub-zero hardening in both stems (base of leaf sheath) and crown meristem complex. Total simple sugar increase, including sucrose, was significantly higher in the crown meristem complex than in the stem.


CONCLUSIONS
Results support the hypothesis that carbohydrate change in mildly frozen plants is tissue specific within crowns and underscore the need to evaluate specific tissue within the crown when correlating the biochemistry of plants with freezing tolerance.}, number={2}, journal={ANNALS OF BOTANY}, author={Livingston, D and Premakumar, R and Tallury, SP}, year={2005}, month={Aug}, pages={331–335} }
 @article{livingston_tallury_premkumar_owens_olien_2005, title={Changes in the histology of cold-hardened oat crowns during recovery from freezing}, volume={45}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2004.0579}, abstractNote={The survival of cereal crops during winter depends primarily on the ability of tissue in the crown to withstand various stresses encountered during freezing. Freeze‐induced damage to specific regions of oat (Avena sativa L.) crowns was evaluated by sectioning plants at various stages of recovery after they had been grown and frozen under controlled conditions. Our results confirmed those reported for barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) that the apical meristem was apparently the tissue in the crown most tolerant of freezing. Photographs of sections during recovery provided evidence that the apical meristem within the crown survived freezing in plants that were rated as nonsurvivors. Closer examination revealed abnormal nuclei in many cells of plants that had been frozen. These cells with condensed and dark red chromatin resembled the description of nuclear pycnosis found in mammalian cells damaged by radiation, extreme abiotic stress, and various carcinogens. The crown meristem complex was separated from the crown and fractionated into two regions: the upper portion of the crown meristem complex, called the apical region, and the lower portion called the crown core. The dry weight of both the apical region and crown core increased during cold‐hardening but the increase in dry weight was higher in the crown core than in the apical region. During cold‐hardening the percentage of total water freezing at −2°C became lower and after 3 wk was 50 and 47% in the apical region and the crown core, respectively. The initial freezing rate of the apical region was higher than that of the crown core and reached equilibrium about 2 h earlier than the crown core. Differences are discussed in relation to the freezing survival of specific tissue.}, number={4}, journal={CROP SCIENCE}, author={Livingston, DP and Tallury, SP and Premkumar, R and Owens, SA and Olien, CR}, year={2005}, pages={1545–1558} }
 @article{tallury_hilu_milla_friend_alsaghir_stalker_quandt_2005, title={Genomic affinities in Arachis section Arachis (Fabaceae): molecular and cytogenetic evidence}, volume={111}, ISSN={["1432-2242"]}, DOI={10.1007/s00122-005-0017-0}, abstractNote={Section Arachis is the largest of nine sections in the genus Arachis and includes domesticated peanut, A. hypogaea L. Most species are diploids (x = 10) with two tetraploids and a few aneuploids. Three genome types have been recognized in this section (A, B and D), but the genomes are not well characterized and relationships of several newly described species are uncertain. To clarify genomic relationships in section Arachis, cytogenetic information and molecular data from amplified fragment length polymorphism (AFLP) and the trnT-F plastid region were used to provide an additional insight into genome composition and species relationships. Cytogenetic information supports earlier observations on genome types of A. cruziana, A. herzogii, A. kempff-mercadoi and A. kuhlmannii but was inconclusive about the genome composition of A. benensis, A. hoehnei, A. ipaensis, A. palustris, A. praecox and A. williamsii. An AFLP dendrogram resolved species into four major clusters and showed A. hypogaea grouping closely with A. ipaensis and A. williamsii. Sequence data of the trnT-F region provided genome-specific information and showed for the first time that the B and D genomes are more closely related to each other than to the A genome. Integration of information from cytogenetics and biparentally and maternally inherited genomic regions show promise in understanding genome types and relationships in Arachis.}, number={7}, journal={THEORETICAL AND APPLIED GENETICS}, publisher={Springer Nature}, author={Tallury, SP and Hilu, KW and Milla, SR and Friend, SA and Alsaghir, M and Stalker, HT and Quandt, D}, year={2005}, month={Nov}, pages={1229–1237} }
 @inbook{tallury_goodman_2000, title={The state of the use of maize genetic diversity in the USA and sub-Saharan Africa}, ISBN={0851994113}, DOI={10.1079/9780851994116.0159}, booktitle={Broadening the genetic bases of crop production}, publisher={New York : CABI Pub}, author={Tallury, S. P. and Goodman, M. M.}, year={2000}, pages={159} }
 @article{tallury_goodman_1999, title={Experimental evaluation of the potential of tropical germplasm for temperate maize improvement}, volume={98}, ISSN={["0040-5752"]}, DOI={10.1007/s001220051039}, number={1}, journal={THEORETICAL AND APPLIED GENETICS}, author={Tallury, SP and Goodman, MM}, year={1999}, month={Jan}, pages={54–61} }
 @article{arumuganathan_tallury_fraser_bruneau_qu_1999, title={Nuclear DNA content of thirteen turfgrass species by flow cytometry}, volume={39}, number={5}, journal={Crop Science}, author={Arumuganathan, K. and Tallury, S. P. and Fraser, M. L. and Bruneau, A. H. and Qu, R.}, year={1999}, pages={1518–1521} }
 @article{tallury_stalker_pattee_1995, title={EARLY REPRODUCTIVE ONTOGENY IN INTERSPECIFIC CROSSES OF ARACHIS-HYPOGAEA AND SECTION ARACHIS SPECIES}, volume={76}, ISSN={["1095-8290"]}, DOI={10.1006/anbo.1995.1113}, abstractNote={Abstract Wild Arachis species have been recognized as sources of resistance to pests and pathogens that infect A. hypogaea L. and cause substantial yield losses. However, utilization of these genetic resources for crop improvement has been difficult. This study was conducted to ( a ) understand the processes of early embryo growth and development in four Arachis species, two A. hypogaea cultivars and their hybrids and ( b ) identify parental compatibilities in reciprocal crosses of A. hypogaea. The results indicated that delayed fertilization beyond 24 h, coupled with slow proembryo growth, leads to embryo abortion in many interspecific crosses. For example, in female A. cardenasii crosses, lack of or delayed fertilization leads to failure to obtain hybrids. When A. batizocoi was used as a female parent, delayed fertilization and the inability of quiescent proembryos to resume growth after soil penetration caused abortion. Embryos of A. hypogaea × A. glandulifera crosses developed normally during the first 21 d after fertilization, but then aborted at a later time. In this study, A. hypogaea was always a better female parent than the wild Arachis species. Increasing the number of pollinations per cross, using the cultivated species as the female parent, utilizing different A. hypogaea varieties, and embryo rescue techniques are suggested to improve the probability of obtaining interspecific hybrids in Arachis.}, number={4}, journal={ANNALS OF BOTANY}, author={TALLURY, SP and STALKER, HT and PATTEE, HE}, year={1995}, month={Oct}, pages={397–404} }