Tzu-Yu Chen

Works (9)

Updated: February 24th, 2025 08:42

2025 article

Bioinformatic, structural, and biochemical analysis leads to the discovery of novel isonitrilases and decodes their substrate selectivity

Hostetler, T., Chen, T.-Y., & Chang, W.-chen. (2025, January 29). RSC CHEMICAL BIOLOGY, Vol. 1.

By: T. Hostetler n, T. Chen n & W. Chang n 

topics (OpenAlex): Chemical Synthesis and Analysis; Peptidase Inhibition and Analysis; Protein Hydrolysis and Bioactive Peptides
Sources: Web Of Science, NC State University Libraries
Added: February 17, 2025

2024 article

Variation in Biosynthesis and Metal-Binding Properties of Isonitrile-Containing Peptides Produced by Mycobacteria versus Streptomyces

Chen, T.-Y., Chen, J., Ruszczycky, M. W., Hilovsky, D., Hostetler, T., Liu, X., … Chang, W.-chen. (2024, March 19). ACS CATALYSIS, Vol. 3.

By: T. Chen n, J. Chen *, M. Ruszczycky*, D. Hilovsky n, T. Hostetler n, X. Liu n, J. Zhou *, W. Chang n 

author keywords: isonitriles; Fe/2OG enzymes; metallophores; Mycobacterium; natural products
topics (OpenAlex): Microbial Natural Products and Biosynthesis; Chemical Synthesis and Analysis; RNA and protein synthesis mechanisms
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Sources: Web Of Science, NC State University Libraries
Added: April 1, 2024

2023 article

Repurposing Iron- and 2-Oxoglutarate-Dependent Oxygenases to Catalyze Olefin Hydration

Wang, B., Lu, Y., Cha, L., Chen, T.-Y., Palacios, P. M., Li, L., … Chen, C. (2023, September 6). ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol. 9.

By: B. Wang *, Y. Lu *, L. Cha n , T. Chen n, P. Palacios*, L. Li *, Y. Guo *, W. Chang n , C. Chen *

author keywords: Biocatalysis; Hydrogen Atom Transfer; Mukaiyama Hydration; Nonheme Fe/2OG-Dependent Oxygenase
topics (OpenAlex): Metal-Catalyzed Oxygenation Mechanisms; Metalloenzymes and iron-sulfur proteins; Metal complexes synthesis and properties
TL;DR: It is shown that various enzymes of this family, when reconstituted with Fe(II) or Fe(III), can catalyze Mukaiyama hydration-a redox neutral transformation and demonstrates that Fe/2OG enzymes have utility in catalysis beyond the current scope of catalytic oxidation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023

2022 journal article

Deciphering the Reaction Pathway of Mononuclear Iron Enzyme-Catalyzed N C Triple Bond Formation in Isocyanide Lipopeptide and Polyketide Biosynthesis

ACS CATALYSIS, 12(4), 2270–2279.

By: T. Chen n, Z. Zheng *, X. Zhang *, J. Chen *, L. Cha n , Y. Tang *, Y. Guo *, J. Zhou * ...

author keywords: isocyanide; oxygenase; natural product; reaction mechanism; desaturation; C-N bond formation
topics (OpenAlex): Synthetic Organic Chemistry Methods; Cyclopropane Reaction Mechanisms; Carbohydrate Chemistry and Synthesis
TL;DR: The results indicate that isocyanide formation likely entails a two-step sequence involving an imine intermediate that undergoes decarboxylation-assisted desaturation to yield the isOCyanide product. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 28, 2022

2022 journal article

Elucidation of divergent desaturation pathways in the formation of vinyl isonitrile and isocyanoacrylate

NATURE COMMUNICATIONS, 13(1).

By: W. Kim *, T. Chen n, L. Cha n , G. Zhou*, K. Xing*, N. Canty n, Y. Zhang*, W. Chang n 

MeSH headings : Iron / metabolism; Ketoglutaric Acids / metabolism; Tyrosine
topics (OpenAlex): Metal-Catalyzed Oxygenation Mechanisms; Enzyme Catalysis and Immobilization; Metalloenzymes and iron-sulfur proteins
TL;DR: It is suggested that a carbocation might be deployed to enable chemically divergent desaturations through decarboxylation and deprotonation and hints at substrate promiscuity of these enzymes. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: September 26, 2022

2021 article

A postreplicative C5-cytosine hypermodification triggered by bacteriophage methyltransferase and hydroxylase

Chen, T.-Y., & Chang, W.-chen. (2021, July 13). PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol. 118.

By: T. Chen n & W. Chang n 

MeSH headings : Bacteriophages / enzymology; Cytosine / metabolism; DNA Replication; Glycosylation; Glycosyltransferases / metabolism; Methyltransferases / metabolism; Mixed Function Oxygenases / metabolism; Substrate Specificity; Viral Proteins / metabolism
topics (OpenAlex): Epigenetics and DNA Methylation; RNA modifications and cancer
TL;DR: Through bioinfor-matic screening, the authors identify and characterize tailoring enzymes, such as glycosyltransferases, that collaborate with phage C5-MT and TET to further elaborate DNA at 5hmC site. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 23, 2021

2021 journal article

Current Understanding toward Isonitrile Group Biosynthesis and Mechanism

CHINESE JOURNAL OF CHEMISTRY, 39(2), 463–472.

By: T. Chen n, J. Chen *, Y. Tang *, J. Zhou *, Y. Guo * & W. Chang n 

author keywords: Biosynthesis; Enzymes; Natural products; Reaction mechanisms; Biotransformations
topics (OpenAlex): Synthesis and Catalytic Reactions; Chemical Synthesis and Analysis; Microbial Natural Products and Biosynthesis
TL;DR: The current biosynthetic pathways and the corresponding enzymes that are responsible for isonitrile-containing natural product generation are summarized and two fundamentally distinctive approaches are discussed. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: March 1, 2021

2021 journal article

Deciphering Pyrrolidine and Olefin Formation Mechanism in Kainic Acid Biosynthesis

ACS CATALYSIS, 11(1), 278–282.

By: T. Chen n, S. Xue *, W. Tsai*, T. Chien *, Y. Guo * & W. Chang n 

author keywords: metalloenzyme; C-H activation; cyclization; desaturation; enzyme mechanism
topics (OpenAlex): Metal-Catalyzed Oxygenation Mechanisms; Metalloenzymes and iron-sulfur proteins; Neurological diseases and metabolism
TL;DR: A comparison of C–H bond activation and cyclization in the isodomoic acid and kainic acid biosynthesis experiments shows that the former is superior to the latter in terms of both molecular complexity and physical properties. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 11, 2021

2020 journal article

Pathway from N-Alkylglycine to Alkylisonitrile Catalyzed by Iron(II) and 2-Oxoglutarate-Dependent Oxygenases

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 59(19), 7367–7371.

By: T. Chen n, J. Chen *, Y. Tang *, J. Zhou *, Y. Guo * & W. Chang n 

Contributors: T. Chen n, J. Chen *, Y. Tang *, J. Zhou *, Y. Guo * & W. Chang n 

author keywords: catalysis; decarboxylation; enzymes; iron; reaction mechanisms
MeSH headings : Catalysis; Glycine / analogs & derivatives; Glycine / chemical synthesis; Hydroxylation; Iron / chemistry; Models, Molecular; Nitriles / chemical synthesis; Nuclear Magnetic Resonance, Biomolecular; Oxidation-Reduction; Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / chemistry; X-Ray Diffraction
topics (OpenAlex): Metal-Catalyzed Oxygenation Mechanisms; Pharmacogenetics and Drug Metabolism; Microbial metabolism and enzyme function
TL;DR: The plausible mechanism of three Fe/2OG enzymes, Sav607, ScoE and SfaA, catalyzed isonitrile formation is reported, and the X-ray structures of iron loaded ScoE in complex with its substrate and the intermediate, along with biochemical and biophysical data reveal that -N≡C bond formation involves two cycles of Fe/ 2OG enzyme catalysis. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: May 8, 2020

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