@article{chen_chen_ruszczycky_hilovsky_hostetler_liu_zhou_chang_2024, title={Variation in Biosynthesis and Metal-Binding Properties of Isonitrile-Containing Peptides Produced by Mycobacteria versus Streptomyces}, volume={3}, ISSN={["2155-5435"]}, DOI={10.1021/acscatal.4c00645}, journal={ACS CATALYSIS}, author={Chen, Tzu-Yu and Chen, Jinfeng and Ruszczycky, Mark W. and Hilovsky, Dalton and Hostetler, Tyler and Liu, Xiaojing and Zhou, Jiahai and Chang, Wei-chen}, year={2024}, month={Mar} }
 @article{wang_lu_cha_chen_palacios_li_guo_chang_chen_2023, title={Repurposing Iron- and 2-Oxoglutarate-Dependent Oxygenases to Catalyze Olefin Hydration}, volume={9}, ISSN={["1521-3773"]}, DOI={10.1002/anie.202311099}, abstractNote={Abstract}, journal={ANGEWANDTE CHEMIE-INTERNATIONAL EDITION}, author={Wang, Bingnan and Lu, Yong and Cha, Lide and Chen, Tzu-Yu and Palacios, Philip M. and Li, Liping and Guo, Yisong and Chang, Wei-chen and Chen, Chuo}, year={2023}, month={Sep} }
 @article{chen_zheng_zhang_chen_cha_tang_guo_zhou_wang_liu_et al._2022, title={Deciphering the Reaction Pathway of Mononuclear Iron Enzyme-Catalyzed N C Triple Bond Formation in Isocyanide Lipopeptide and Polyketide Biosynthesis}, volume={12}, ISSN={["2155-5435"]}, DOI={10.1021/acscatal.1c04869}, abstractNote={Despite the diversity of reactions catalyzed by 2-oxoglutarate-dependent nonheme iron (Fe/2OG) enzymes identified in recent years, only a limited number of these enzymes have been investigated in mechanistic detail. In particular, several Fe/2OG-dependent enzymes capable of catalyzing isocyanide formation have been reported. While the glycine moiety has been identified as a biosynthon for the isocyanide group, how the actual conversion is effected remains obscure. To elucidate the catalytic mechanism, we characterized two previously unidentified (AecA and AmcA) along with two known (ScoE and SfaA) Fe/2OG-dependent enzymes that catalyze N≡C triple bond installation using synthesized substrate analogues and potential intermediates. Our results indicate that isocyanide formation likely entails a two-step sequence involving an imine intermediate that undergoes decarboxylation-assisted desaturation to yield the isocyanide product. Results obtained from the in vitro experiments are further supported by mutagenesis, the product-bound enzyme structure, and in silico analysis.}, number={4}, journal={ACS CATALYSIS}, author={Chen, Tzu-Yu and Zheng, Ziyang and Zhang, Xuan and Chen, Jinfeng and Cha, Lide and Tang, Yijie and Guo, Yisong and Zhou, Jiahai and Wang, Binju and Liu, Hung-wen and et al.}, year={2022}, month={Feb}, pages={2270–2279} }
 @article{kim_chen_cha_zhou_xing_canty_zhang_chang_2022, title={Elucidation of divergent desaturation pathways in the formation of vinyl isonitrile and isocyanoacrylate}, volume={13}, ISSN={["2041-1723"]}, DOI={10.1038/s41467-022-32870-4}, abstractNote={Abstract}, number={1}, journal={NATURE COMMUNICATIONS}, author={Kim, Wantae and Chen, Tzu-Yu and Cha, Lide and Zhou, Grace and Xing, Kristi and Canty, Nicholas Koenig and Zhang, Yan and Chang, Wei-Chen}, year={2022}, month={Sep} }
 @article{chen_chang_2021, title={A postreplicative C5-cytosine hypermodification triggered by bacteriophage methyltransferase and hydroxylase}, volume={118}, ISSN={["0027-8424"]}, DOI={10.1073/pnas.2109992118}, abstractNote={Viruses of bacteria, also known as bacteriophages, harbor the greatest diversity of DNA modifications identified to date. To fight against restriction endo-nucleases of their hosts, bacteriophages modify their genomic DNA through introduction of various moieties including amino acids, polyamines, and sugars (1, 2). A series of transformations are involved in DNA base modification. Followed by formation of hydroxymethyl pyrimidine nucleotides, which are utilized by DNA polymerase, replication and postreplicative modifications furnish installation of these moieties onto the DNA poly-mer (3 – 7). Burke et al. (8) show that bacteriophages re-sort to C5-cytosine methyltransferase (C5-MT) and 5-methylcytosine dioxygenase ten-eleven translocation enzyme (TET) as an alternative mechanism to postrepli-catively form hydroxymethylcytosine on DNA. The bacteriophage TET enables site-specific hydroxylation of 5-methylcytosine (5mC), installed by C5-MT, to produce 5-hydroxymethylcytosine (5hmC). Through bioinfor-matic screening, the authors identify and characterize tailoring enzymes, such as glycosyltransferases, that collaborate with phage C5-MT and TET to further elaborate DNA at 5hmC site.}, number={28}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Chen, Tzu-Yu and Chang, Wei-chen}, year={2021}, month={Jul} }
 @article{chen_chen_tang_zhou_guo_chang_2021, title={Current Understanding toward Isonitrile Group Biosynthesis and Mechanism}, volume={39}, ISSN={["1614-7065"]}, DOI={10.1002/cjoc.202000448}, abstractNote={Abstract}, number={2}, journal={CHINESE JOURNAL OF CHEMISTRY}, author={Chen, Tzu-Yu and Chen, Jinfeng and Tang, Yijie and Zhou, Jiahai and Guo, Yisong and Chang, Wei-chen}, year={2021}, month={Feb}, pages={463–472} }
 @article{chen_xue_tsai_chien_guo_chang_2021, title={Deciphering Pyrrolidine and Olefin Formation Mechanism in Kainic Acid Biosynthesis}, volume={11}, ISSN={["2155-5435"]}, url={http://dx.doi.org/10.1021/acscatal.0c03879}, DOI={10.1021/acscatal.0c03879}, abstractNote={Metalloenzyme-catalyzed cyclization involving C–H bond activation is a powerful strategy to construct molecular complexity found in natural product biosynthesis. In the isodomoic acid and kainic ac...}, number={1}, journal={ACS CATALYSIS}, publisher={American Chemical Society (ACS)}, author={Chen, Tzu-Yu and Xue, Shan and Tsai, Wei-Chih and Chien, Tun-Cheng and Guo, Yisong and Chang, Wei-chen}, year={2021}, month={Jan}, pages={278–282} }
 @article{chen_chen_tang_zhou_guo_chang_2020, title={Pathway from N-Alkylglycine to Alkylisonitrile Catalyzed by Iron(II) and 2-Oxoglutarate-Dependent Oxygenases}, volume={59}, ISSN={["1521-3773"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85081226966&partnerID=MN8TOARS}, DOI={10.1002/anie.201914896}, abstractNote={Abstract}, number={19}, journal={ANGEWANDTE CHEMIE-INTERNATIONAL EDITION}, author={Chen, Tzu-Yu and Chen, Jinfeng and Tang, Yijie and Zhou, Jiahai and Guo, Yisong and Chang, Wei-chen}, year={2020}, month={May}, pages={7367–7371} }