Troy Ghashghaei

Works (45)

Updated: April 3rd, 2024 17:16

2023 journal article

Bulk and mosaic deletions of Egfr reveal regionally defined gliogenesis in the developing mouse forebrain

ISCIENCE, 26(3).

By: X. Zhang n, G. Xiao n, C. Johnson n, Y. Cai n, Z. Horowitz n, C. Mennicke n, R. Coffey *, M. Haider*  ...

TL;DR: Subtractive bulk ventral and dorsal forebrain deletions of Egfr uncovered significant and permanent decreases in oligodendrogenesis and myelination in the cortex and corpus callosum, and a regenerative population of gliogenic progenitors in the mouse forebrain may compensate for the missing EGFR-dependent dorsal glia in the bulk Egfr-deleted forebrain. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 5, 2023

2023 journal article

COMBINe enables automated detection and classification of neurons and astrocytes in tissue-cleared mouse brains

CELL REPORTS METHODS, 3(4).

By: Y. Cai n, X. Zhang n, C. Li n, H. Ghashghaei  n & A. Greenbaum n

MeSH headings : Animals; Mice; Astrocytes; Excipients; Microscopy, Fluorescence; Neurons; Prosencephalon
TL;DR: An automated workflow to map sparsely labeled neurons and astrocytes in genetically distinct mouse forebrains using mosaic analysis with double markers (MADM) is presented and the regional and subregional effects of MADM-based deletion of the epidermal growth factor receptor (EGFR) are quantitatively analyzed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: May 30, 2023

2023 journal article

Deep learning-based adaptive optics for light sheet fluorescence microscopy

BIOMEDICAL OPTICS EXPRESS, 14(6), 2905–2919.

By: M. Rai, C. Li*, H. Ghashghaei  n & A. Greenbaum*

TL;DR: It is shown that the application of correction using a deformable mirror greatly improves image quality and two conceptually different network architectures are compared; one that shares convolutional features and another that estimates each aberration independently. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: July 24, 2023

2022 article

Deep learning-based autofocus method enhances image quality in light-sheet fluorescence microscopy: publishers note (vol 12, pg 5214, 2021)

LI, C., Moatti, A., Zhang, X., Ghashghaei, H. T., & Greenbaum, A. (2022, January 1). BIOMEDICAL OPTICS EXPRESS, Vol. 13, pp. 373–373.

By: C. Li n, A. Moatti n , X. Zhang n, H. Ghashghaei  n & A. Greenbaum n

UN Sustainable Development Goal Categories
Source: Web Of Science
Added: January 10, 2022

2022 journal article

Illumination angle correction during image acquisition in light-sheet fluorescence microscopy using deep learning

BIOMEDICAL OPTICS EXPRESS, 13(2), 888–901.

By: C. Li n, M. Rai, H. Ghashghaei  n & A. Greenbaum n

TL;DR: This study provides a framework that can correct the angle of the light-sheet and improve the overall image quality in high-resolution LSFM 3D image acquisition. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: February 14, 2022

2022 article

Phosphorylation-dependent proteome of Marcks in ependyma during aging and behavioral homeostasis in the mouse forebrain

Muthusamy, N., Williams, T. I., O'Toole, R., Brudvig, J. J., Adler, K. B., Weimer, J. M., … Ghashghaei, H. T. (2022, January 24). GEROSCIENCE, Vol. 1.

By: N. Muthusamy n, T. Williams n , R. O'Toole *, J. Brudvig *, K. Adler n, J. Weimer *, D. Muddiman n , H. Ghashghaei  n

Contributors: N. Muthusamy n, T. Williams n , R. O’Toole *, J. Brudvig *, K. Adler n, J. Weimer *, D. Muddiman n , H. Ghashghaei  n

author keywords: Aging; Behavior; Clca3; Ependymal cells; Forebrain; Marcks; Mass spectrometry; Neuroinflammation
MeSH headings : Mice; Animals; Phosphorylation; Ependyma / metabolism; Intracellular Signaling Peptides and Proteins / metabolism; Myristoylated Alanine-Rich C Kinase Substrate / metabolism; Proteome / metabolism; Membrane Proteins / metabolism; Protein Kinase C / metabolism; Homeostasis; Prosencephalon / metabolism; Mammals / metabolism
TL;DR: This study provides first line of evidence for potential mechanisms that may mediate differential Marcks functions in young and old ECs, and their effect on forebrain homeostasis during aging. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 31, 2022

2021 journal article

Deep learning-based autofocus method enhances image quality in light-sheet fluorescence microscopy

BIOMEDICAL OPTICS EXPRESS, 12(8), 5214–5226.

By: C. Li n, A. Moatti n , X. Zhang n, H. Ghashghaei  n & A. Greenabum

TL;DR: A deep learning-based autofocus framework that can estimate the position of the objective-lens focal plane relative to the light-sheet, based on two defocused images is presented, which outperforms or provides comparable results with the best traditional aut ofocus method on small and large image patches respectively. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 16, 2021

2021 journal article

Detection and classification of neurons and glial cells in the MADM mouse brain using RetinaNet

PLOS ONE, 16(9).

By: Y. Cai n, X. Zhang n, S. Kovalsky *, H. Ghashghaei  n & A. Greenbaum n

MeSH headings : Animals; Brain; Cell Differentiation; Mice; Neuroglia; Neurons
TL;DR: Object detection networks (neural networks) are utilized to detect and classify targets in complex microscopy images, while simplifying data annotation, and merging the predictions of the two computational models significantly improved the automated cell counting of glial clusters. (via Semantic Scholar)
UN Sustainable Development Goal Categories
8. Decent Work and Economic Growth (OpenAlex)
Source: Web Of Science
Added: February 28, 2022

2020 journal article

Ependyma-expressed CCN1 restricts the size of the neural stem cell pool in the adult ventricular-subventricular zone

EMBO JOURNAL, 39(5).

By: J. Wu *, W. Tian*, Y. Liu *, H. Wan*, J. Zheng *, X. Wang *, H. Pan *, J. Li * ...

author keywords: CCN1; EGFR; ependymal cells; neural stem cells; niche
MeSH headings : Adult Stem Cells / physiology; Animals; Brain; Cysteine-Rich Protein 61 / genetics; Cysteine-Rich Protein 61 / metabolism; Ependyma / cytology; Ependyma / metabolism; ErbB Receptors / genetics; ErbB Receptors / metabolism; Female; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Neural Stem Cells / cytology; Neural Stem Cells / metabolism; Neurogenesis / physiology; Signal Transduction
TL;DR: It is shown that ependyma‐derived matricellular protein CCN1 (cellular communication network factor 1) negatively regulates niche capacity and NSC number in the adult ventricular–subventricular zone (V‐SVZ) of the adult brain to maintain the proper niche capacity of the V‐VZ. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: February 17, 2020

2020 journal article

Sp2 regulates late neurogenic but not early expansive divisions of neural stem cells underlying population growth in the mouse cortex

DEVELOPMENT, 147(4).

By: C. Johnson n & H. Ghashghaei  n

author keywords: Corticogenesis; Mitosis; MADM; Neurogenesis; Sp2; Neural stem cell
MeSH headings : Alleles; Animals; Cell Differentiation; Cell Division; Cell Lineage; Cell Proliferation; Cerebral Cortex / embryology; Female; Gene Deletion; Genetic Markers; Male; Mice; Mice, Transgenic; Mitosis; Mutation; Neural Stem Cells / cytology; Phenotype; Sp2 Transcription Factor / genetics; Sp2 Transcription Factor / physiology
TL;DR: It is found that loss of Sp2 in progenitors undergoing neurogenic divisions results in prolonged mitosis due to extension of early mitotic stages, and mechanistic differences between the early expansive and later neurogenic periods of cortical development are revealed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: April 20, 2020

2020 article

The people behind the papers - Caroline Johnson and Troy Ghashghaei

Johnson, C., & Ghashghaei, T. (2020, February). DEVELOPMENT, Vol. 147.

By: C. Johnson n & T. Ghashghaei n

MeSH headings : Animals; Brain / embryology; Cell Proliferation; Developmental Biology / history; Developmental Biology / methods; History, 21st Century; Mice; Mitosis; Sp2 Transcription Factor / physiology
TL;DR: This new paper in Development investigates the molecular control of mitosis in these two stages, using simultaneous labelling and gene knockout in clones in the developing mouse brain. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: April 20, 2020

2018 journal article

Foxj1 expressing ependymal cells do not contribute new cells to sites of injury or stroke in the mouse forebrain

SCIENTIFIC REPORTS, 8.

By: N. Muthusamy n, A. Brumm*, X. Zhang n, S. Carmichael * & H. Ghashghaei  n

MeSH headings : Animals; Cell Differentiation / physiology; Ependyma / metabolism; Forkhead Transcription Factors / metabolism; Mice; Neurogenesis / physiology; Neuroglia / metabolism; Neurons / metabolism; Prosencephalon / metabolism; Stem Cells / metabolism; Stroke / metabolism
TL;DR: Using a cell specific genetic approach, Foxj1+ ependymal cells are ruled out as stem cells participating in neurogenesis and gliogenesis in response to acute injury or stroke in the mouse forebrain. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2018 journal article

MARCKS regulates neuritogenesis and interacts with a CDC42 signaling network

SCIENTIFIC REPORTS, 8.

By: J. Brudvig *, J. Cain*, R. Sears *, G. Schmidt-Grimminger*, E. Wittchen*, K. Adler n, H. Ghashghaei  n, J. Weimer *

MeSH headings : Actins / metabolism; Animals; Cytoskeleton / metabolism; Female; Male; Mice; Mice, Inbred C57BL; Microtubules / metabolism; Myristoylated Alanine-Rich C Kinase Substrate / metabolism; Neurites / metabolism; Neurons / cytology; Neurons / metabolism; Phosphorylation; Primary Cell Culture; Protein Binding; Pseudopodia / metabolism; Signal Transduction; cdc42 GTP-Binding Protein / metabolism
TL;DR: It is proposed that MARCKS is uniquely positioned to regulate CDC42 localization and interactions within specialized cellular compartments, such as nascent neurites. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: October 19, 2018

2018 journal article

To scratch an itch: Establishing a mouse model to determine active brain areas involved in acute histaminergic itch

IBRO Reports, 5, 67–73.

By: S. Ehling  n, A. Butler n, S. Thi  n, H. Ghashghaei  n & W. Bäumer  n

author keywords: c-fos; Itch; Brain; Medial habenula; Histamine H4 receptor
TL;DR: The specific histamine H4 receptor agonist ST-1006 induces acute itch in mice and selective H4R activation in the skin increases neuronal activity in the medial habenula. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Crossref
Added: February 21, 2020

2017 journal article

Developmentally defined forebrain circuits regulate appetitive and aversive olfactory learning

NATURE NEUROSCIENCE, 20(1), 20–23.

By: N. Muthusamy n, X. Zhang n, C. Johnson n, P. Yadav* & H. Ghashghaei  n

MeSH headings : Animals; Appetitive Behavior / physiology; Avoidance Learning / physiology; Behavior, Animal / physiology; Conditioning, Classical / physiology; Male; Mice; Neurogenesis / physiology; Neurons / physiology; Olfactory Cortex / physiology; Smell / physiology
TL;DR: It is demonstrated that chemogenetic inactivation of a subset of forebrain and olfactory neurons generated at birth disrupts responses to an aversive odor, revealing that developmentally defined sets of neurons may differentially participate in hedonic aspects of sensory learning. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury

SCIENTIFIC REPORTS, 7.

By: Y. Ren *, Y. Ao*, . Timothy M. O'Shea *, J. Burda *, A. Bernstein*, A. Brumm*, N. Muthusamy n, H. Ghashghaei  n ...

MeSH headings : Animals; Astrocytes / physiology; Cell Differentiation; Cicatrix / physiopathology; Disease Models, Animal; Ependyma / physiopathology; Mice; Mice, Transgenic; Neural Stem Cells / physiology; Spinal Cord Injuries / physiopathology
TL;DR: It is shown that ependyma contribution of progeny after SCI is minimal, local and dependent on direct ependymal injury, indicating that ependedyma are not a major source of endogenous neural stem cells or neuroprotective astrocytes afterSCI. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Mosaic analysis with double markers reveals distinct sequential functions of Lgl1 in neural stem cells

Neuron (Cambridge, Mass.), 94(3), 517-.

By: R. Beattie*, M. Postiglione*, L. Burnett *, S. Laukoter *, C. Streicher*, F. Pauler *, G. Xiao n, O. Klezovitch* ...

MeSH headings : Animals; Cell Polarity; Cell Proliferation / genetics; Embryo, Mammalian / metabolism; Glycoproteins / genetics; Glycoproteins / metabolism; Mice; Mice, Knockout; Microscopy, Confocal; Neocortex / embryology; Neocortex / growth & development; Neocortex / pathology; Neural Stem Cells / cytology; Neural Stem Cells / metabolism; Neurogenesis / genetics; Neuroglia / cytology; Neuroglia / metabolism; Neurons / cytology; Neurons / metabolism
TL;DR: Results suggest that NSC-mediated neuron and glia production is tightly regulated through the concerted interplay of sequential Lgl1-dependent global and cell intrinsic mechanisms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018

2017 journal article

Regulation of cytokinesis during corticogenesis: focus on the midbody

FEBS Letters, 591(24), 4009–4026.

By: C. Johnson  n, C. Wright n & H. Ghashghaei  n

author keywords: abscission; asymmetric; cell division; corticogenesis; cytokinesis; midbody; neurogenesis; symmetric
MeSH headings : Animals; Body Patterning / physiology; Cell Differentiation / physiology; Cell Division / physiology; Cell Movement / physiology; Cerebral Cortex / cytology; Cerebral Cortex / embryology; Cytokinesis / physiology; Humans; Neural Stem Cells / cytology; Neural Stem Cells / physiology; Neurogenesis / physiology
TL;DR: This review describes the midbody and its constituent proteins, as well as the known consequences of their manipulation during cortical development, and the potential functional relevance of midbody mechanisms is discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Crossref
Added: February 21, 2020

2017 journal article

Stomach curvature is generated by left-right asymmetric gut morphogenesis

DEVELOPMENT, 144(8), 1477–1483.

By: A. Davis n, N. Amin n, C. Johnson n, K. Bagley n, H. Ghashghaei  n & N. Nascone-Yoder n

author keywords: Left-right; Asymmetry; Stomach; Gut; Morphogenesis; Pitx2; Xenopus; Mouse
MeSH headings : Animals; Body Patterning; Endoderm / embryology; Endoderm / metabolism; Epithelium / embryology; Epithelium / metabolism; Homeodomain Proteins / metabolism; Mice; Rotation; Signal Transduction; Stomach / anatomy & histology; Stomach / embryology; Transcription Factors / metabolism; Xenopus / embryology
TL;DR: It is shown that the mechanisms that drive the curvature of the stomach are intrinsic to the gut tube itself, and this findings have implications for how LR patterning manifests distinct types of morphological asymmetries in different contexts. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Neural Stem Cells to Cerebral Cortex: Emerging Mechanisms Regulating Progenitor Behavior and Productivity

JOURNAL OF NEUROSCIENCE, 36(45), 11394–11401.

By: N. Dwyer *, B. Chen *, S. Chou *, S. Hippenmeyer *, L. Nguyen * & H. Ghashghaei  n

author keywords: cortical development; Cux2; cytokinesis; Fezf2; Kif20b; Lhx2; lineage; MADM; microcephaly; mouse; neurogenesis; Sp2; UPR
MeSH headings : Animals; Cell Differentiation / physiology; Cell Proliferation / physiology; Cerebral Cortex / cytology; Cerebral Cortex / physiology; Cytokinesis / physiology; Humans; Neurogenesis / physiology; Neuronal Plasticity / physiology; Neurons / cytology; Neurons / physiology
TL;DR: Four aspects of corticogenesis are focused on: cytokinesis events that follow apical mitoses of NSCs; coordinating abscission with delamination from the apical membrane; timing of neurogenesis and its indirect regulation through emergence of intermediate progenitors; and capacity of single N SCs to generate the correct number and laminar fate of cortical neurons. (via Semantic Scholar)
UN Sustainable Development Goal Categories
8. Decent Work and Economic Growth (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Neurotypic cell attachment and growth on III-nitride lateral polarity structures

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 58, 1194–1198.

By: L. Bain n, R. Kirste n, C. Johnson  n, H. Ghashghaei  n, R. Collazo n  & A. Ivanisevic n

author keywords: Gallium nitride; Cell adhesion; Neurons; Semiconductor; Polarity
MeSH headings : Aluminum Compounds / chemistry; Aluminum Compounds / pharmacology; Animals; Cell Adhesion / drug effects; Cell Differentiation / drug effects; Gallium / chemistry; Gallium / pharmacology; Neurons / cytology; Neurons / drug effects; PC12 Cells; Rats; Semiconductors
TL;DR: The designed material configuration is further explored for biochemical sensing, and the lateral polarity scheme may provide a route in assessing the non-specific protein adsorption to this varying nano-topography that drives the subsequent cell response. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

TAK1 determines susceptibility to endoplasmic reticulum stress and leptin resistance in the hypothalamus

Journal of Cell Science, 129(9), 1855–1865.

By: K. Sai  n, S. Morioka  n, G. Takaesu  n, N. Muthusamy n, H. Ghashghaei  n, H. Hanafusa *, K. Matsumoto *, J. Ninomiya-Tsuji n

author keywords: TAK1; Endoplasmic reticulum stress; Leptin; Hypothalamus; Obesity
MeSH headings : Animals; Dietary Fats / adverse effects; Dietary Fats / pharmacology; Endoplasmic Reticulum Stress; Hyperphagia / chemically induced; Hyperphagia / genetics; Hyperphagia / metabolism; Hyperphagia / pathology; Hypothalamus / metabolism; Hypothalamus / pathology; Leptin / genetics; Leptin / metabolism; MAP Kinase Kinase Kinases / genetics; MAP Kinase Kinase Kinases / metabolism; Mice; Mice, Knockout; Obesity / chemically induced; Obesity / genetics; Obesity / metabolism; Obesity / pathology; Sterol Regulatory Element Binding Proteins / genetics; Sterol Regulatory Element Binding Proteins / metabolism
TL;DR: It is found that deletion of Tak1 increased ER volume and facilitated ER-stress tolerance in cultured cells, which was mediated by upregulation of sterol-regulatory-element-binding protein (SREBP)-dependent lipogenesis and central nervous system (CNS) deletion upregulated SREBP-target lipogenic genes and blocked ER stress in the hypothalamus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, Crossref
Added: August 6, 2018

2016 journal article

TransOmic analysis of forebrain sections in Sp2 conditional knockout embryonic mice using IR-MALDESI imaging of lipids and LC-MS/MS label-free proteomics

Analytical and Bioanalytical Chemistry, 408(13), 3453–3474.

By: P. Loziuk n, F. Meier n, C. Johnson  n, H. Ghashghaei  n & D. Muddiman n 

author keywords: IR-MALDESI; Proteomics; Lipidomics; Mass spectrometry imaging; Embryonic brain
MeSH headings : Animals; Chromatography, Liquid / methods; Lipids / analysis; Mass Spectrometry / methods; Mice; Mice, Knockout; Prosencephalon / embryology; Prosencephalon / metabolism; Sp2 Transcription Factor / genetics; Spectrophotometry, Infrared / methods; Transcriptome
TL;DR: An integrated approach for systematic analysis of the “lipidome” in tissue revealed many specific changes to fundamental cellular processes and biosynthetic pathways in the cerebral cortices of Sp2-cKO embryos. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Influence of Desorption Conditions on Analyte Sensitivity and Internal Energy in Discrete Tissue or Whole Body Imaging by IR-MALDESI

Journal of The American Society for Mass Spectrometry, 26(6), 899–910.

By: E. Rosen n, M. Bokhart n, H. Ghashghaei  n & D. Muddiman n 

author keywords: IR-MALDESI; Mass spectrometry imaging; Laser desorption whole body imaging; Lipids; Xenobiotics
MeSH headings : Anesthetics, Local / analysis; Anesthetics, Local / pharmacokinetics; Animals; Cocaine / analysis; Cocaine / pharmacokinetics; Infrared Rays; Lipid Metabolism; Lipids / analysis; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Whole Body Imaging / methods
TL;DR: Optimized desorption conditions have been used to conduct an untargeted lipidomic analysis of whole body sagittal sections of neonate mouse, with enhanced lipid coverage received by varying the laser wavelength used for mass spectrometry imaging (MSI). (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

MARCKS-dependent mucin clearance and lipid metabolism in ependymal cells are required for maintenance of forebrain homeostasis during aging

AGING CELL, 14(5), 764–773.

By: N. Muthusamy n, L. Sommerville n, A. Moeser n, D. Stumpo *, P. Sannes n, K. Adler n, P. Blackshear *, J. Weimer *, H. Ghashghaei  n

author keywords: aging; barrier function; Clca3; cerebral cortex; ependymal cells; lipid droplets; mucin; oxidative stress
MeSH headings : Aging / metabolism; Animals; Ependyma / cytology; Ependyma / metabolism; Homeostasis; Intracellular Signaling Peptides and Proteins / deficiency; Intracellular Signaling Peptides and Proteins / metabolism; Lipid Metabolism; Membrane Proteins / deficiency; Membrane Proteins / metabolism; Mice; Mucins / metabolism; Myristoylated Alanine-Rich C Kinase Substrate; Oxidative Stress; Prosencephalon / metabolism
TL;DR: It is demonstrated that metabolic and barrier functions in ECs decline significantly during aging in mice, and this work constitutes a conceptually new paradigm in the potential role of ECs in the initiation of various conditions and diseases in the aging brain. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Transplantation of GABAergic Interneurons into the Neonatal Primary Visual Cortex Reduces Absence Seizures in Stargazer Mice

CEREBRAL CORTEX, 25(9), 2970–2979.

By: M. Hammad*, S. Schmidt *, X. Zhang*, R. Bray, F. Frohlich * & H. Ghashghaei  n

author keywords: absence seizure; MGE transplantation; network activity; Stargazer; visual cortex
MeSH headings : Animals; Calcium Channels / genetics; Calcium Channels / metabolism; Disease Models, Animal; Embryo, Mammalian; Epilepsy, Absence / genetics; Epilepsy, Absence / surgery; GABAergic Neurons / physiology; GABAergic Neurons / transplantation; Glutamate Decarboxylase / metabolism; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; In Vitro Techniques; Median Eminence / cytology; Membrane Potentials / drug effects; Membrane Potentials / physiology; Mice; Mice, Inbred C57BL; Mice, Transgenic; Neocortex / cytology; Nerve Tissue Proteins / metabolism; Proto-Oncogene Proteins c-fos / metabolism; Treatment Outcome; Visual Cortex / transplantation; gamma-Aminobutyric Acid / metabolism
TL;DR: It is reported that transplantation of MGE cells into the primary visual cortex (V1) of Stg mice significantly reduces AE episodes and lowers mortality and presents MGE grafting in the V1 as a possible clinical approach in the treatment of AE. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Unique Glycan Signatures Regulate Adeno-Associated Virus Tropism in the Developing Brain

JOURNAL OF VIROLOGY, 89(7), 3976–3987.

By: G. Murlidharan*, T. Corriher*, H. Ghashghaei  n & A. Asokan *

MeSH headings : Animals; Brain / virology; Dependovirus / physiology; Mice, Inbred BALB C; Receptors, Virus / metabolism; Sialic Acids / metabolism; Transduction, Genetic; Viral Tropism
TL;DR: It is postulate that glycan signatures associated with host development can be exploited to redirect novel AAV vectors to specific cell types in the brain and suggest that carbohydrate content can be exploiting to regulate viral transport and tropism in the head. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 journal article

A Knock-in Foxj1(CreERT2:: GFP)Mouse for Recombination in Epithelial Cells with Motile Cilia

GENESIS, 52(4), 350–358.

By: N. Muthusamy n, A. Vijayakumar n, G. Cheng * & H. Ghashghaei  n

author keywords: Foxj1; CreERT2::GFP; knock-in; ependymal cells; motile cilia; epithelial cells
MeSH headings : Animals; Cell Lineage; Choroid Plexus / cytology; Choroid Plexus / metabolism; Cilia / physiology; Epithelial Cells / metabolism; Epithelial Cells / ultrastructure; Female; Forkhead Transcription Factors / biosynthesis; Forkhead Transcription Factors / genetics; Gene Expression; Gene Knock-In Techniques; Green Fluorescent Proteins / biosynthesis; Green Fluorescent Proteins / genetics; Integrases / genetics; Male; Mice, 129 Strain; Mice, Inbred C57BL; Mice, Transgenic; Recombination, Genetic; Tamoxifen / pharmacology; Transcriptional Activation / drug effects
TL;DR: The generation of an inducible knock-in Foxj1CreERT2::GFP knock‐in mouse is reported, which reliably induces Cre‐mediated recombination for genetic studies in epithelial cells with motile cilia throughout embryonic and postnatal development. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Identification of neuronal loci involved with displays of affective aggression in NC900 mice

BRAIN STRUCTURE & FUNCTION, 218(4), 1033–1049.

By: D. Nehrenberg n, A. Sheikh n & H. Ghashghaei  n

author keywords: Aggression; Violence; Neuronal circuits; Neural networks; Neuronal activity; Amygdala; Nucleus accumbens; Hypothalamus; Limbic thalamus; Parafascicular thalamic nucleus; Paraventricular thalamic nucleus; Zona incerta; Anxiety disorders; Mouse; c-Fos
MeSH headings : Aggression / physiology; Analysis of Variance; Animals; Brain Mapping; Cell Count; Immunohistochemistry; Male; Mice; Mice, Inbred Strains; Microscopy, Confocal; Neural Pathways / physiology; Neurons / metabolism; Prosencephalon / cytology; Prosencephalon / physiology; Proto-Oncogene Proteins c-fos / metabolism
TL;DR: A detailed anatomical map of neuronal activity in the forebrain of two inbred lines of mice selected for low (NC100) and high (NC900) affective aggression is provided to speculate that neuronal activities in analogous networks may be disrupted in humans prone to maladaptive affectsive aggression. (via Semantic Scholar)
UN Sustainable Development Goal Categories
16. Peace, Justice and Strong Institutions (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Neural development is dependent on the function of specificity protein 2 in cell cycle progression

DEVELOPMENT, 140(3), 552–561.

By: H. Liang n, G. Xiao n, H. Yin n, S. Hippenmeyer *, J. Horowitz n & H. Ghashghaei  n

author keywords: Neurogenesis; Neural stem cells; Neural progenitors; Sp2; Cell cycle; M phase; Mouse
MeSH headings : Animals; Brain / cytology; Brain / embryology; Brain / metabolism; Cell Count; Cell Cycle; Cell Proliferation; Crosses, Genetic; Embryo Implantation; Embryo, Mammalian / cytology; Embryo, Mammalian / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Eye Proteins / genetics; Eye Proteins / metabolism; Female; Genetic Markers; Homeodomain Proteins / genetics; Homeodomain Proteins / metabolism; Homologous Recombination; Intermediate Filament Proteins / genetics; Intermediate Filament Proteins / metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Nerve Tissue Proteins / genetics; Nerve Tissue Proteins / metabolism; Nestin; Neural Stem Cells / cytology; Neural Stem Cells / metabolism; Neurogenesis; Neurons / cytology; Neurons / metabolism; PAX6 Transcription Factor; Paired Box Transcription Factors / genetics; Paired Box Transcription Factors / metabolism; Repressor Proteins / genetics; Repressor Proteins / metabolism; Sp2 Transcription Factor / genetics; Sp2 Transcription Factor / metabolism; Stem Cell Niche; Transplantation Chimera / embryology; Transplantation Chimera / metabolism
TL;DR: It is demonstrated that neural stem cells and intermediate neural progenitor cells (NPCs) employ a zinc-finger transcription factor specificity protein 2 (Sp2) as a cell cycle regulator in two temporally and spatially distinct progenitors domains. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic neural progenitors

Biology Open, 1(12), 1200–1203.

By: H. Liang n, S. Hippenmeyer * & H. Ghashghaei  n

TL;DR: It is found that the rate of Nestin-cre driven recombination only reaches sufficiently high levels in NSCs and NPCs during late embryonic and early postnatal periods, which is important when commercially available cre lines are considered for directing recombination to embryonic NSCS and NPCs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Crossref
Added: July 20, 2019

2011 journal article

Specification of a Foxj1-Dependent Lineage in the Forebrain Is Required for Embryonic-to-Postnatal Transition of Neurogenesis in the Olfactory Bulb

JOURNAL OF NEUROSCIENCE, 31(25), 9368–9382.

By: B. Jacquet n, N. Muthusamy n, L. Sommerville n, G. Xiao n, H. Liang n, Y. Zhang *, M. Holtzman *, H. Ghashghaei  n

MeSH headings : Animals; Forkhead Transcription Factors / metabolism; Mice; Mice, Knockout; Neurogenesis / physiology; Neurons / physiology; Olfactory Bulb / embryology; Olfactory Bulb / physiology; Prosencephalon / embryology; Prosencephalon / physiology
TL;DR: For the first time, this study identifies the time- and region-specific activity of a perinatal progenitor domain that is required for transition and progression of OB neurogenesis from the embryonic-to-postnatal periods. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

Journal of Visualized Experiments, (46).

By: B. Jacquet n, P. Ruckart n & H. Ghashghaei  n

MeSH headings : Animals; Brain / anatomy & histology; Brain / cytology; Cell Movement / physiology; Image Processing, Computer-Assisted / methods; Lateral Ventricles / cytology; Mice; Neural Stem Cells / cytology; Neurogenesis / physiology; Neurons / cytology; Olfactory Bulb / cytology; Organ Culture Techniques / methods
TL;DR: An organotypic slice assay optimized for postnatal brain tissue, in which the extracellular conditions closely mimic the in vivo environment for migrating neuroblasts within the RMS, is described. (via Semantic Scholar)
Source: Crossref
Added: July 20, 2019

2010 journal article

Development of a Model of Sacrocaudal Spinal Cord Injury in Cloned Yucatan MiniPigs for Cellular Transplantation Research

CELLULAR REPROGRAMMING, 12(6), 689–697.

By: J. Lim n, J. Piedrahita n , L. Jackson n, T. Ghashghaei n & N. Olby n 

MeSH headings : Animals; Behavior, Animal; Cauda Equina / pathology; Cauda Equina / surgery; Cell Culture Techniques; Cells, Cultured; Cloning, Organism; Green Fluorescent Proteins / metabolism; Humans; Models, Animal; Nerve Regeneration / physiology; Nuclear Transfer Techniques; Recovery of Function / physiology; Sacrum; Spinal Cord / pathology; Spinal Cord / surgery; Spinal Cord Injuries / pathology; Stem Cell Transplantation / methods; Swine; Swine, Miniature / surgery
TL;DR: It is demonstrated that transection of the sacrocaudal spinal cord in Yucatan SCNT clones produces profound, quantifiable neurological deficits restricted to the tail, which represents a powerful research tool to investigate the effect of cellular transplantation on axonal regeneration and functional recovery. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Analysis of neuronal proliferation, migration and differentiation in the postnatal brain using equine infectious anemia virus-based lentiviral vectors

GENE THERAPY, 16(8), 1021–1033.

By: B. Jacquet n, M. Patel*, M. Iyengar n, H. Liang n, B. Therit n, R. Salinas-Mondragon, C. Lai *, J. Olsen*, E. Anton*, H. Ghashghaei  n

author keywords: adult neural stem cells; adult neurogenesis; lentivirus; EIAV; olfactory bulb; neuronal migration
MeSH headings : Adult Stem Cells / physiology; Animals; Brain / cytology; Brain / physiology; Cell Differentiation; Cell Movement; Cell Proliferation; Defective Viruses; Gene Transfer Techniques; Genetic Vectors; Infectious Anemia Virus, Equine / genetics; Mice; Neural Stem Cells / physiology; Neurogenesis; Neurons / physiology; Olfactory Bulb / cytology; Stem Cell Niche / genetics; Transduction, Genetic
TL;DR: It is shown that a replication-incompetent Equine Infectious Anemia Virus (EIAV) is highly suitable for stable and persistent gene transfer to adult neural stem cells and ishighly suitable for discovery and assessment of mechanisms that regulate proliferation, migration and differentiation in the postnatal brain. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

ClC-2 is required for rapid restoration of epithelial tight junctions in ischemic-injured murine jejunum

Experimental Cell Research, 315(1), 110–118.

By: P. Nighot n, A. Moeser n, K. Ryan n, T. Ghashghaei n & A. Blikslager n 

Contributors: P. Nighot n, A. Moeser n, K. Ryan n, T. Ghashghaei n & A. Blikslager n 

author keywords: Tight junction; Intestinal permeability; Ischemia; ClC-2
MeSH headings : Animals; CLC-2 Chloride Channels; Cell Membrane / metabolism; Chloride Channels / deficiency; Chloride Channels / metabolism; Epithelium / metabolism; Epithelium / pathology; Epithelium / ultrastructure; Fluorescent Antibody Technique; Immunoprecipitation; Ischemia / metabolism; Ischemia / physiopathology; Jejunum / blood supply; Jejunum / pathology; Jejunum / ultrastructure; Mannitol / metabolism; Membrane Proteins / metabolism; Mice; Mice, Knockout; Occludin; Protein Transport; Tight Junctions / metabolism; Tight Junctions / ultrastructure; Wound Healing
TL;DR: A pivotal role is demonstrated in recovery of the intestinal epithelium barrier by anchoring assembly of tight junctions following ischemic injury in ClC-2(-/-) mice. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID, NC State University Libraries
Added: August 6, 2018

2009 journal article

Deficient NRG1-ERBB signaling alters social approach: relevance to genetic mouse models of schizophrenia

JOURNAL OF NEURODEVELOPMENTAL DISORDERS, 1(4), 302–312.

By: S. Moy *, H. Ghashghaei *, R. Nonneman*, J. Weimer *, Y. Yokota*, D. Lee*, C. Lai *, D. Threadgill *, E. Anton*

author keywords: Grin1; Growth factor; NMDA receptor; Schizophrenia; Sociability; Social novelty
TL;DR: Findings provide evidence that social behavior is mediated by NRG1-ERBB signaling, which is implicated in genetic susceptibility for schizophrenia and has been implicated in asociality and altered social interaction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

FoxJ1-dependent gene expression is required for differentiation of radial glia into ependymal cells and a subset of astrocytes in the postnatal brain

DEVELOPMENT, 136(23), 4021–4031.

By: B. Jacquet n, R. Salinas-Mondragon, H. Liang n, B. Therit n, J. Buie n, M. Dykstra n, K. Campbell*, L. Ostrowski*, S. Brody*, H. Ghashghaei  n

author keywords: Subventricular zone; Adult stem cell niche; Ependymal cells; Astrocytes; FoxJ1; Mouse
MeSH headings : Animals; Astrocytes / cytology; Astrocytes / metabolism; Astrocytes / ultrastructure; Brain / cytology; Brain / metabolism; Cell Differentiation / physiology; Cells, Cultured; Ependyma / cytology; Ependyma / metabolism; Ependyma / ultrastructure; Fluorescent Antibody Technique, Direct; Forkhead Transcription Factors / genetics; Forkhead Transcription Factors / metabolism; Gene Expression; Immunohistochemistry; In Situ Hybridization; Mice; Mice, Knockout; Neuroglia / cytology; Neuroglia / physiology; Neuroglia / ultrastructure
TL;DR: It is suggested that time- and cell-specific expression of FoxJ1 in the brain acts on an array of target genes to regulate the differentiation of ependymal cells and a small subset of astrocytes in the adult stem cell niche. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 review

Neuronal migration in the adult brain: are we there yet?

[Review of ]. NATURE REVIEWS NEUROSCIENCE, 8(2), 141–151.

By: H. Ghashghaei  n, C. Lai * & E. Anton*

MeSH headings : Animals; Brain / cytology; Cell Movement / physiology; Neurons / physiology
TL;DR: This review aims to summarize the patterns, mechanisms and functional significance of neuronal migration in the postnatal brain, with an emphasis on the migratory events that persist in the mature brain. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Radial Glial Dependent and Independent Dynamics of Interneuronal Migration in the Developing Cerebral Cortex

PLoS ONE, 2(8), e794.

By: Y. Yokota*, H. Ghashghaei *, C. Han*, H. Watson*, K. Campbell * & E. Anton*

Ed(s): T. Chan-Ling

MeSH headings : Animals; Cell Differentiation; Cell Movement / physiology; Cell Polarity; Cells, Cultured; Cerebral Cortex / cytology; Cerebral Cortex / embryology; Cerebral Cortex / physiology; Embryo, Mammalian / metabolism; Immunohistochemistry; Interneurons / cytology; Interneurons / metabolism; Mice; Mice, Transgenic; Neuroglia / cytology; Neuroglia / metabolism
TL;DR: The observations suggest that interactions with radial glial scaffold and localized migration within the expanding cerebral wall may play essential roles in the guidance and placement of interneurons in the developing cerebral cortex. (via Semantic Scholar)
Source: Crossref
Added: July 20, 2019

2007 journal article

Reinduction of ErbB2 in astrocytes promotes radial glial progenitor identity in adult cerebral cortex

Genes & Development, 21(24), 3258–3271.

By: H. Ghashghaei  n, J. Weimer *, R. Schmid *, Y. Yokota*, K. McCarthy*, B. Popko *, E. Anton*

MeSH headings : Animals; Astrocytes / cytology; Astrocytes / metabolism; Cell Proliferation; Cerebral Cortex / cytology; Cerebral Cortex / metabolism; Immunohistochemistry; Mice; Mice, Transgenic; Neuroglia / cytology; Receptor, ErbB-2 / biosynthesis; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: It is shown that the in vivo induction of the tyrosine kinase receptor, ErbB2, in mature astrocytes enables a subset of them to regain radial glial identity in the mature cerebral cortex. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

Sequence of information processing for emotions based on the anatomic dialogue between prefrontal cortex and amygdala

NeuroImage, 34(3), 905–923.

By: H. Ghashghaei *, C. Hilgetag * & H. Barbas *

MeSH headings : Amygdala / cytology; Amygdala / physiology; Animals; Emotions / physiology; Female; Information Storage and Retrieval / methods; Macaca mulatta; Male; Microscopy, Fluorescence / methods; Models, Anatomic; Models, Neurological; Nerve Net / cytology; Nerve Net / physiology; Neural Pathways / cytology; Neural Pathways / physiology; Neurons / cytology; Neurons / physiology; Prefrontal Cortex / cytology; Prefrontal Cortex / physiology
TL;DR: Investigation of the largely unknown extent and laminar relationship of prefrontal input-output zones linked with the amygdala using neural tracers injected in the amygdala in rhesus monkeys indicates that the connections between prefrontal cortices and the amygdala follow similar patterns as corticocortical connections. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2006 journal article

The role of neuregulin-ErbB4 interactions on the proliferation and organization of cells in the subventricular zone

Proceedings of the National Academy of Sciences, 103(6), 1930–1935.

By: H. Ghashghaei *, J. Weber *, L. Pevny*, R. Schmid *, M. Schwab *, K. Lloyd *, D. Eisenstat *, C. Lai *, E. Anton*

author keywords: adult neurogenesis; rostral migratory stream; schizophrenia
MeSH headings : Animals; Cell Differentiation; Cell Movement; Cell Proliferation; Enzyme Activation; ErbB Receptors / deficiency; ErbB Receptors / genetics; ErbB Receptors / metabolism; Ligands; Mice; Neuregulins / metabolism; Olfactory Bulb / cytology; Olfactory Bulb / metabolism; Prosencephalon / cytology; Prosencephalon / metabolism; Protein Binding; Receptor, ErbB-4; Time Factors
TL;DR: NRG2 is identified as a factor capable of promoting SVZ proliferation, leading to the formation of new neurons in vivo, indicating that different NRG ligands affect distinct populations of differentiating neural precursors in the neurogenic regions of the mature forebrain. (via Semantic Scholar)
Source: Crossref
Added: July 20, 2019

2005 journal article

752. Equine Infectious Anemia Virus Pseudotyped with the Vesicular Stomatitis Virus G-Protein, Preferentially Targets Neural Precursors in the Adult Mouse Brain

Molecular Therapy, 11(Supplement 1), S292.

By: T. Ghashghaei, M. Patel, J. Olsen & E. Anton

TL;DR: The preferential targeting of GFAP+ astrocytes in the SVZ by the EIAV lentivirus is potentially useful for the molecular manipulation of these stem cells. (via Semantic Scholar)
Source: Crossref
Added: December 29, 2020

2004 journal article

Receptor tyrosine kinase ErbB4 modulates neuroblast migration and placement in the adult forebrain

Nature Neuroscience, 7(12), 1319–1328.

By: E. Anton*, H. Ghashghaei *, J. Weber *, C. McCann*, T. Fischer*, I. Cheung*, M. Gassmann *, A. Messing * ...

MeSH headings : Animals; Animals, Newborn; Cell Movement / physiology; ErbB Receptors / biosynthesis; ErbB Receptors / genetics; ErbB Receptors / physiology; Mice; Mice, Knockout; Neurons / cytology; Neurons / enzymology; Prosencephalon / cytology; Prosencephalon / enzymology; Prosencephalon / growth & development; Receptor, ErbB-4
TL;DR: It is suggested that ErbB4 activation helps to regulate the organization of neural chains that form the rostral migratory stream and influences the differentiation of olfactory interneuronal precursors. (via Semantic Scholar)
Source: Crossref
Added: July 20, 2019

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