@article{esparza-diaz_marconi_avila_villanueva_2021, title={Persistence of the Exotic Mirid Nesidiocoris tenuis (Hemiptera: Miridae) in South Texas}, url={https://doi.org/10.3390/insects12080715}, DOI={10.3390/insects12080715}, abstractNote={The Rio Grande Valley is one of the most productive agricultural areas in the U.S and is located in the southernmost part of Texas. In October 2013, we detected an exotic plant bug, Nesidiocoris tenuis Reuter (Hemiptera: Miridae: Bryocorinae) occurring in the region. Nesidiocoris tenuis has zoophytophagous habits; however, in the absence of insect prey, it feeds on its plant hosts. After its morphological and genetic identification, this study monitored the population of N. tenuis in its introduction phase in commercial fields and corroborated its establishment in research fields for three years. Populations of N. tenuis were high during the fall and low during winter. This study found that N. tenuis populations were higher in tomato fields as compared to adjacent pepper, okra, and squash fields, indicating its host preferences during the introduction phase. Recurrent population growth patterns suggest that N. tenuis was established in Rio Grande Valley with permanent populations in tomato fields. In addition, N. tenuis populations were affected by tomato cultivar selection and by plastic mulch color. The presence of N. tenuis could establish a new trophic insect relationship for vegetable production. However, it is unknown if the presence of N. tenuis may help to control pests of economic importance, such as whiteflies in cotton, or become a pest on sesame, an emerging crop.}, journal={Insects}, author={Esparza-Diaz, Gabriela and Marconi, Thiago and Avila, Carlos A. and Villanueva, Raul T.}, year={2021}, month={Aug} }
 @article{cholula_silva_marconi_thomasson_solorzano_enciso_2020, title={Forecasting Yield and Lignocellulosic Composition of Energy Cane Using Unmanned Aerial Systems}, url={https://www.mdpi.com/2073-4395/10/5/718}, DOI={10.3390/agronomy10050718}, abstractNote={Crop monitoring and appropriate agricultural management practices of elite germplasm will enhance bioenergy’s efficiency. Unmanned aerial systems (UAS) may be a useful tool for this purpose. The objective of this study was to assess the use of UAS with true color and multispectral imagery to predict the yield and total cellulosic content (TCC) of newly created energy cane germplasm. A trial was established in the growing season of 2016 at the Texas A&M AgriLife Research Center in Weslaco, Texas, where 15 energy cane elite lines and three checks were grown on experimental plots, arranged in a complete block design and replicated four times. Four flights were executed at different growth stages in 2018, at the first ratoon crop, using two multi-rotor UAS: the DJI Phantom 4 Pro equipped with RGB camera and the DJI Matrice 100, equipped with multispectral sensor (SlantRange 3p). Canopy cover, canopy height, NDVI (Normalized Difference Vegetation Index), and ExG (Excess Green Index) were extracted from the images and used to perform a stepwise regression to obtain the yield and TCC models. The results showed a good agreement between the predicted and the measured yields (R2 = 0.88); however, a low coefficient of determination was found between the predicted and the observed TCC (R2 = 0.30). This study demonstrated the potential application of UAS to estimate energy cane yield with high accuracy, enabling plant breeders to phenotype larger populations and make selections with higher confidence.}, journal={Agronomy}, author={Cholula, Uriel and Silva, Jorge A. and Marconi, T. G. and Thomasson, J. Alex and Solorzano, Jorge and Enciso, Juan}, year={2020}, month={May} }
 @article{kandel_marconi_badillo-vargas_enciso_zapata_lazcano_crosby_avila_2020, title={Yield and fruit quality of high-tunnel tomato cultivars produced during the off-season in South Texas}, volume={272}, url={https://doi.org/10.1016/j.scienta.2020.109582}, DOI={10.1016/j.scienta.2020.109582}, abstractNote={The Rio Grande Valley (RGV) of South Texas is one of the most diverse vegetable-producing regions in Texas. Traditionally, tomatoes in the RGV have been grown in open field during the spring and fall, but the production area has declined significantly due to the high pressure of pests and diseases. RGV open-field cultivation during winter is limited to cold-tolerant vegetables such spinach and cabbage due to the risk of cold days and nights. As an alternative, high tunnels offer an environment protected against pests and diseases, wind, and low temperatures. Here, we studied the feasibility of RGV high-tunnel tomato production to identify the planting date, tomato type, and cultivars best suited for producing high-quality tomatoes during winter. The high tunnel maintained temperatures above 21 °C during chilly days without providing additional heat. In addition, whitefly and thrips populations remained low during most of the cropping season, although pesticide control was required at crop establishment to lower their populations. Overall, higher yields were achieved in October compared to November planting and from determinate compared to indeterminate cultivars. Determinate tomato cultivars TAM Hot-Ty, Mykonos, and TAM HT1 transplanted in October produced up to 96.4, 129.13, and 85.98 t/ha, respectively. Among the cultivars tested, TAM Hot-Ty and TAM HT1 possessed the highest concentrations of soluble solids, with ° Brix >5 %. In an organoleptic test, fruits from high tunnel tomatoes were ranked higher than Texas supermarket tomatoes imported from other regions in terms of flavor, color, and overall characteristics. This study demonstrates that tomato high-tunnel production offers a viable alternative to open-field production in South Texas and could provide a source of high-quality locally produced tomatoes to Texas and nearby markets.}, journal={Scientia Horticulturae}, publisher={Elsevier BV}, author={Kandel, Devi R. and Marconi, Thiago G. and Badillo-Vargas, Ismael E. and Enciso, Juan and Zapata, Samuel D. and Lazcano, Carlos A. and Crosby, Kevin and Avila, Carlos A.}, year={2020}, month={Oct}, pages={109582} }
 @article{silva_costa_marconi_barreto_solís-gracia_park_glynn_2018, title={Agronomic and molecular characterization of wild germplasm saccharum spontaneum for sugarcane and energycane breeding purposes}, volume={75}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85044262051&partnerID=MN8TOARS}, DOI={10.1590/1678-992x-2017-0028}, abstractNote={Among the species related to sugarcane, Saccharum spontaneum (L.) is a wild species with the greatest potential as a source of genetic variation to cope with biomass production in harsh environments. Due to its high yield, early vigor, ratooning ability, low input requirements and tolerance to various biotic and abiotic stresses, sugarcane breeders have shown interest in its contribution, as a donor of genes, to the development of high biomass energy canes. The conservation, evaluation and utilization of the genetic variability of S. spontaneum available in germplasm collections are critical for breeding, but, given the aggressive rhizomatous growth habit and the ability to propagate via seed dispersal, S. spontaneum is classified as a noxious weed in several nations, including the U.S.A. As a result, field trials are restrictive and few phenotypic analyses have been carried out on these collections. In the present study, a subset of 130 S. spontaneum accessions obtained from the World Collection of Sugarcane and Related Grasses in Miami, FL has been characterized phenotypically - with either morphological and agronomic traits (including composition analysis) or reaction to abiotic stress and genotypically - molecular markers (Simple Sequence Repeats - SSR). Using these data, a core collection has been established, genotypes with positive agronomic traits have been identified and are being used as parents for hybridization crosses, aimed at genetic improvement of sugarcane and energycane.}, number={4}, journal={Scientia Agricola}, author={Silva, J.A.G. and Costa, P.M.A. and Marconi, T.G. and Barreto, E.J.S. and Solís-Gracia, N. and Park, J.-W. and Glynn, N.C.}, year={2018}, pages={329–338} }
 @book{mancini_cardoso-silva_costa_marconi_garcia_souza_2017, title={New developments in sugarcane genetics and genomics}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85034569506&partnerID=MN8TOARS}, DOI={10.1007/978-3-319-49826-3_9}, journal={Advances of Basic Science for Second Generation Bioethanol from Sugarcane}, author={Mancini, M.C. and Cardoso-Silva, C.B. and Costa, E.A. and Marconi, T.G. and Garcia, A.A.F. and Souza, A.P.}, year={2017}, pages={159–174} }
 @article{alves_silva costa_amôr divino miranda_ramos_marconi_santos oliveira_castilho_sartorato_2016, title={Analysis of mitochondrial alterations in Brazilian patients with sensorineural hearing loss using MALDI-TOF mass spectrometry}, volume={17}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84970005823&partnerID=MN8TOARS}, DOI={10.1186/s12881-016-0303-5}, abstractNote={Mutations in the mitochondrial DNA (mtDNA) have been associated with aminoglycoside-induced and nonsyndromic deafness in different populations. In the present study, we investigated the contribution of mutations in mitochondrial genes to the etiology of hearing loss in a Brazilian sample.Using mass spectrometry genotyping technology, combined with direct sequencing, 50 alterations previously described in 14 mitochondrial genes were screened in 152 patients with sensorineural hearing loss and in104 normal hearing controls.Fifteen known mitochondrial alterations were detected (G709A, A735G, A827G, G988A, A1555G, T4363C, T5628C, T5655C, G5821A, C7462T, G8363A, T10454C, G12236A, T1291C, G15927A). Pathogenic mutations in MT-RNR1 and MT-TK genes were detected in 3 % (5/152) of the patients with hearing loss.This study contributed to show the spectrum of mitochondrial variants in Brazilian patients with hearing loss. Frequency of A1555G was relatively high (2.6 %), indicating that this mutation is an important cause of hearing loss in our population. This work reports for the first time the investigation and the detection of the tRNA(Lys) G8363A mutation in Brazilian patients with maternally inherited sensorineural hearing loss.}, number={1}, journal={BMC Medical Genetics}, author={Alves, R.M. and Silva Costa, S.M. and Amôr Divino Miranda, P.M. and Ramos, P.Z. and Marconi, T.G. and Santos Oliveira, G. and Castilho, A.M. and Sartorato, E.L.}, year={2016} }
 @article{costa_anoni_mancini_santos_marconi_gazaffi_pastina_perecin_mollinari_xavier_et al._2016, title={QTL mapping including codominant SNP markers with ploidy level information in a sugarcane progeny}, volume={211}, ISSN={0014-2336 1573-5060}, url={http://dx.doi.org/10.1007/s10681-016-1746-7}, DOI={10.1007/s10681-016-1746-7}, abstractNote={Abstract}, number={1}, journal={Euphytica}, publisher={Springer Science and Business Media LLC}, author={Costa, E. A. and Anoni, C. O. and Mancini, M. C. and Santos, F. R. C. and Marconi, T. G. and Gazaffi, R. and Pastina, M. M. and Perecin, D. and Mollinari, M. and Xavier, M. A. and et al.}, year={2016}, month={Jul}, pages={1–16} }
 @article{park_benatti_marconi_yu_solis-gracia_mora_da silva_2015, title={Cold responsive gene expression profiling of sugarcane and Saccharum spontaneum with functional analysis of a cold inducible Saccharum homolog of NOD26-like intrinsic protein to salt and water stress}, volume={10}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84929380261&partnerID=MN8TOARS}, DOI={10.1371/journal.pone.0125810}, abstractNote={Transcriptome analysis of sugarcane hybrid CP72-1210 (cold susceptible) and Saccharum spontaneum TUS05-05 (cold tolerant) using Sugarcane Assembled Sequences (SAS) from SUCEST-FUN Database showed that a total of 35,340 and 34,698 SAS genes, respectively, were expressed before and after chilling stress. The analysis revealed that more than 600 genes are differentially expressed in each genotype after chilling stress. Blast2Go annotation revealed that the major difference in gene expression profiles between CP72-1210 and TUS05-05 after chilling stress are present in the genes related to the transmembrane transporter activity. To further investigate the relevance of transmembrane transporter activity against abiotic stress tolerance, a S. spontaneum homolog of a NOD26-like major intrinsic protein gene (SspNIP2) was selected for functional analysis, of which expression was induced after chilling stress in the cold tolerant TUS05-05. Quantitative real-time PCR showed that SspNIP2 expression was increased ~2.5 fold at 30 minutes after cold treatment and stayed induced throughout the 24 hours of cold treatment. The amino acid sequence analysis of the cloned SspNIP2 confirmed the presence of six transmembrane domains and two NPA (Asn-Pro-Ala) motifs, signature features of major intrinsic protein families. Amino acid analysis confirmed that four amino acids, comprising the ar/R (aromatic residue/arginine) region responsible for the substrate specificity among MIPs, are conserved among monocot silicon transporters and SspNIP2. Salinity stress test on SspNIP2 transgenic tobacco plants resulted in more vigorous transgenic lines than the non-transgenic tobacco plants, suggesting some degree of tolerance to salt stress conferred by SspNIP2. SspNIP2-transgenic plants, exposed to 2 weeks of water stress without irrigation, developed various degrees of water stress symptom. The water stress test confirmed that the SspNIP2 transgenic lines had lower evapotranspiration rates than non-transgenic lines, suggesting that SspNIP2 transgenic lines showed a slight tolerance to the early water stress compared to wild type plants.}, number={5}, journal={PLoS ONE}, author={Park, J.-W. and Benatti, T.R. and Marconi, T. and Yu, Q. and Solis-Gracia, N. and Mora, V. and Da Silva, J.A.}, year={2015} }
 @article{garcia_mollinari_marconi_serang_silva_vieira_vicentini_costa_mancini_garcia_et al._2013, title={SNP genotyping allows an in-depth characterisation of the genome of sugarcane and other complex autopolyploids}, volume={3}, ISSN={2045-2322}, url={http://dx.doi.org/10.1038/srep03399}, DOI={10.1038/srep03399}, abstractNote={Many plant species of great economic value (e.g., potato, wheat, cotton, and sugarcane) are polyploids. Despite the essential roles of autopolyploid plants in human activities, our genetic understanding of these species is still poor. Recent progress in instrumentation and biochemical manipulation has led to the accumulation of an incredible amount of genomic data. In this study, we demonstrate for the first time a successful genetic analysis in a highly polyploid genome (sugarcane) by the quantitative analysis of single-nucleotide polymorphism (SNP) allelic dosage and the application of a new data analysis framework. This study provides a better understanding of autopolyploid genomic structure and is a sound basis for genetic studies. The proposed methods can be employed to analyse the genome of any autopolyploid and will permit the future development of high-quality genetic maps to assist in the assembly of reference genome sequences for polyploid species.}, number={1}, journal={Scientific Reports}, publisher={Springer Science and Business Media LLC}, author={Garcia, Antonio A. F. and Mollinari, Marcelo and Marconi, Thiago G. and Serang, Oliver R. and Silva, Renato R. and Vieira, Maria L. C. and Vicentini, Renato and Costa, Estela A. and Mancini, Melina C. and Garcia, Melissa O. S. and et al.}, year={2013}, month={Dec} }
 @article{palhares_rodrigues-morais_van sluys_domingues_maccheroni_jordão_souza_marconi_mollinari_gazaffi_et al._2012, title={A novel linkage map of sugarcane with evidence for clustering of retrotransposon-based markers}, volume={13}, ISSN={1471-2156}, url={http://dx.doi.org/10.1186/1471-2156-13-51}, DOI={10.1186/1471-2156-13-51}, abstractNote={Abstract}, number={1}, journal={BMC Genetics}, publisher={Springer Science and Business Media LLC}, author={Palhares, Alessandra C and Rodrigues-Morais, Taislene B and Van Sluys, Marie-Anne and Domingues, Douglas S and Maccheroni, Walter, Jr and Jordão, Hamilton, Jr and Souza, Anete P and Marconi, Thiago G and Mollinari, Marcelo and Gazaffi, Rodrigo and et al.}, year={2012}, month={Jun} }
 @article{guidugli_ferreira-ramos_sousa_cidade_marconi_mestriner_groppo_alzate-marin_2012, title={Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers}, volume={11}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84930473469&partnerID=MN8TOARS}, DOI={10.4238/2012.January.9.2}, abstractNote={Metrodorea nigra (Rutaceae) is an endemic Brazilian tree of great ecological importance, frequently found in the submontane regions of ombrophilous dense and semideciduous forests. This tree is useful for reforesting degraded areas and the wood can be employed in construction. We developed 12 microsatellite markers from a genomic library enriched for GA/CA repeats, for this species. Polymorphisms were assessed in 40 trees of a highly fragmented population found in Cravinhos, State of São Paulo, in southeastern Brazil. Among the 12 loci, 8 were polymorphic and only one had fixed alleles in this population. The number of alleles per locus and expected heterozygosity ranged from 2 to 11 and from 0.190 to 0.889, respectively. These results revealed moderate levels of genetic variation in M. nigra population when compared to other tropical species. Additionally, transferability of the 12 primers was tested in seven other Brazilian Rutaceae tree species (endemics: M. stipularis, Galipea jasminiflora, Esenbeckia leiocarpa and non-endemics: E. febrifuga, E. grandiflora, Balfourodendron riedelianum, Zanthoxylum riedelianum). Transferability ranged among species, but at least 8 loci (~67%) amplified in M. stipularis, demonstrating a high potential for transferring microsatellite markers between species of the same genus in the Rutaceae family.}, number={1}, journal={Genetics and Molecular Research}, author={Guidugli, M.C. and Ferreira-Ramos, R. and Sousa, A.C.B. and Cidade, F.W. and Marconi, T.G. and Mestriner, M.A. and Groppo, M. and Alzate-Marin, A.L.}, year={2012}, pages={10–16} }
 @article{guidugli_ferreira-ramos_sousa_cidade_marconi_mestriner_groppo_alzate-marin_2012, title={Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers}, volume={11}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84855692937&partnerID=MN8TOARS}, DOI={http://dx.doi.org/10.4238/2012.January.9.2}, abstractNote={Metrodorea nigra (Rutaceae) is an endemic Brazilian tree of great ecological importance, frequently found in the submontane regions of ombrophilous dense and semideciduous forests.This tree is useful for reforesting degraded areas and the wood can be employed in construction.We developed 12 microsatellite markers from a genomic library enriched for GA/CA repeats, for this species.Polymorphisms were assessed in 40 trees of a highly fragmented population found in Cravinhos, State of São Paulo, in southeastern Brazil.Among the 12 loci, 8 were polymorphic and only one had fixed alleles in this population.The number of alleles per locus and expected heterozygosity ranged from 2 to 11 and from 0.190 to 0.889, respectively.These results revealed mod-©FUNPEC-RP www.funpecrp.com.br}, number={1}, journal={Genetics and Molecular Research}, author={Guidugli, M.C. and Ferreira-Ramos, R. and Sousa, A.C.B. and Cidade, F.W. and Marconi, T.G. and Mestriner, M.A. and Groppo, M. and Alzate-Marin, A.L.}, year={2012}, pages={10–16} }
 @article{guidugli_ferreira-ramos_sousa_cidade_2012, title={Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers}, volume={11}, number={1}, journal={Genetics and Molecular Research}, author={Guidugli, M. C. and Ferreira-Ramos, R. and Sousa, A. C. B. and Cidade, F. W.}, year={2012}, pages={10–16} }
 @article{marconi_costa_miranda_mancini_cardoso-silva_oliveira_pinto_mollinari_garcia_souza_2011, title={Functional markers for gene mapping and genetic diversity studies in sugarcane}, volume={4}, ISSN={1756-0500}, url={http://dx.doi.org/10.1186/1756-0500-4-264}, DOI={10.1186/1756-0500-4-264}, abstractNote={The database of sugarcane expressed sequence tags (EST) offers a great opportunity for developing molecular markers that are directly associated with important agronomic traits. The development of new EST-SSR markers represents an important tool for genetic analysis. In sugarcane breeding programs, functional markers can be used to accelerate the process and select important agronomic traits, especially in the mapping of quantitative traits loci (QTL) and plant resistant pathogens or qualitative resistance loci (QRL). The aim of this work was to develop new simple sequence repeat (SSR) markers in sugarcane using the sugarcane expressed sequence tag (SUCEST database).A total of 365 EST-SSR molecular markers with trinucleotide motifs were developed and evaluated in a collection of 18 genotypes of sugarcane (15 varieties and 3 species). In total, 287 of the EST-SSRs markers amplified fragments of the expected size and were polymorphic in the analyzed sugarcane varieties. The number of alleles ranged from 2-18, with an average of 6 alleles per locus, while polymorphism information content values ranged from 0.21-0.92, with an average of 0.69. The discrimination power was high for the majority of the EST-SSRs, with an average value of 0.80. Among the markers characterized in this study some have particular interest, those that are related to bacterial defense responses, generation of precursor metabolites and energy and those involved in carbohydrate metabolic process.These EST-SSR markers presented in this work can be efficiently used for genetic mapping studies of segregating sugarcane populations. The high Polymorphism Information Content (PIC) and Discriminant Power (DP) presented facilitate the QTL identification and marker-assisted selection due the association with functional regions of the genome became an important tool for the sugarcane breeding program.}, number={1}, journal={BMC Research Notes}, publisher={Springer Science and Business Media LLC}, author={Marconi, Thiago G and Costa, Estela A and Miranda, Hercília RCAN and Mancini, Melina C and Cardoso-Silva, Cláudio B and Oliveira, Karine M and Pinto, Luciana R and Mollinari, Marcelo and Garcia, Antônio AF and Souza, Anete P}, year={2011}, month={Jul}, pages={264} }
 @article{siqueira_marconi_bonatelli_zucchi_veasey_2011, title={New microsatellite loci for water yam (Dioscorea alata, Dioscoreaceae) and cross-amplification for other Dioscorea species}, volume={98}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84858407459&partnerID=MN8TOARS}, DOI={10.3732/ajb.1000513}, abstractNote={• Premise of the study: Dioscorea alata L. is one of the most widely distributed species of the genus in the humid and semihumid tropics and is associated with traditional agriculture. Only a few microsatellite markers have been developed so far for this and other Dioscorea species.}, number={6}, journal={American Journal of Botany}, author={Siqueira, M.V.B.M. and Marconi, T.G. and Bonatelli, M.L. and Zucchi, M.I. and Veasey, E.A.}, year={2011}, pages={e144–e146} }
 @article{hotta_lembke_domingues_ochoa_cruz_melotto-passarin_marconi_santos_mollinari_margarido_et al._2010, title={The Biotechnology Roadmap for Sugarcane Improvement}, volume={3}, ISSN={1935-9756 1935-9764}, url={http://dx.doi.org/10.1007/s12042-010-9050-5}, DOI={10.1007/s12042-010-9050-5}, number={2}, journal={Tropical Plant Biology}, publisher={Springer Science and Business Media LLC}, author={Hotta, Carlos T. and Lembke, Carolina G. and Domingues, Douglas S. and Ochoa, Edgar A. and Cruz, Guilherme M. Q. and Melotto-Passarin, Danila M. and Marconi, Thiago G. and Santos, Melissa O. and Mollinari, Marcelo and Margarido, Gabriel R. A. and et al.}, year={2010}, month={Apr}, pages={75–87} }
 @article{hotta_lembke_domingues_ochoa_cruz_melotto-passarin_marconi_santos_mollinari_margarido_2010, title={The biotechnology roadmap for sugarcane improvement}, volume={3}, number={2}, journal={Tropical Plant Biology}, author={Hotta, C. T. and Lembke, C. G. and Domingues, D. S. and Ochoa, E. A. and Cruz, G. M. Q. and Melotto-Passarin, D. M. and Marconi, T. G. and Santos, M. O. and Mollinari, M. and Margarido, G. R. A.}, year={2010}, pages={75–87} }
 @article{oliveira_pinto_marconi_mollinari_ulian_chabregas_falco_burnquist_garcia_souza_2009, title={Characterization of new polymorphic functional markers for sugarcane}, volume={52}, ISSN={0831-2796 1480-3321}, url={http://dx.doi.org/10.1139/G08-105}, DOI={10.1139/G08-105}, abstractNote={ Expressed sequence tags (ESTs) offer the opportunity to exploit single, low-copy, conserved sequence motifs for the development of simple sequence repeats (SSRs). The authors have examined the Sugarcane Expressed Sequence Tag database for the presence of SSRs. To test the utility of EST-derived SSR markers, a total of 342 EST–SSRs, which represent a subset of over 2005 SSR-containing sequences that were located in the sugarcane EST database, could be designed from the nonredundant SSR-positive ESTs for possible use as potential genic markers. These EST–SSR markers were used to screen 18 sugarcane ( Saccharum spp.) varieties. A high proportion (65.5%) of the above EST–SSRs, which gave amplified fragments of foreseen size, detected polymorphism. The number of alleles ranged from 2 to 24 with an average of 7.55 alleles per locus, while polymorphism information content values ranged from 0.16 to 0.94, with an average of 0.73. The ability of each set of EST–SSR markers to discriminate between varieties was generally higher than the polymorphism information content analysis. When tested for functionality, 82.1% of these 224 EST–SSRs were found to be functional, showing homology to known genes. As the EST–SSRs are within the expressed portion of the genome, they are likely to be associated to a particular gene of interest, improving their utility for genetic mapping; identification of quantitative trait loci, and comparative genomics studies of sugarcane. The development of new EST–SSR markers will have important implications for the genetic analysis and exploitation of the genetic resources of sugarcane and related species and will provide a more direct estimate of functional diversity. }, number={2}, journal={Genome}, publisher={Canadian Science Publishing}, author={Oliveira, K. M. and Pinto, L. R. and Marconi, T. G. and Mollinari, M. and Ulian, E. C. and Chabregas, S. M. and Falco, M. C. and Burnquist, W. and Garcia, A. A.F. and Souza, A. P.}, editor={Golding, BrianEditor}, year={2009}, month={Feb}, pages={191–209} }
 @article{oliveira_pinto_marconi_margarido_pastina_teixeira_figueira_ulian_garcia_souza_2007, title={Functional integrated genetic linkage map based on EST-markers for a sugarcane (Saccharum spp.) commercial cross}, volume={20}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-34548154066&partnerID=MN8TOARS}, DOI={10.1007/s11032-007-9082-1}, number={3}, journal={Molecular Breeding}, author={Oliveira, K.M. and Pinto, L.R. and Marconi, T.G. and Margarido, G.R.A. and Pastina, M.M. and Teixeira, L.H.M. and Figueira, A.V. and Ulian, E.C. and Garcia, A.A.F. and Souza, A.P.}, year={2007}, pages={189–208} }
 @article{pinto_oliveira_marconi_garcia_ulian_de souza_2006, title={Characterization of novel sugarcane expressed sequence tag microsatellites and their comparison with genomic SSRs}, volume={125}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-33746444258&partnerID=MN8TOARS}, DOI={10.1111/j.1439-0523.2006.01227.x}, abstractNote={Abstract}, number={4}, journal={Plant Breeding}, author={Pinto, L.R. and Oliveira, K.M. and Marconi, T. and Garcia, A.A.F. and Ulian, E.C. and De Souza, A.P.}, year={2006}, pages={378–384} }