Works (5)
Updated: July 31st, 2023 21:15
2020 journal article
Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs
NUCLEIC ACIDS RESEARCH, 48(10), 5624–5638.
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / classification; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / classification; CRISPR-Associated Proteins / genetics; CRISPR-Associated Proteins / metabolism; DNA Cleavage; Endodeoxyribonucleases / chemistry; Endodeoxyribonucleases / classification; Endodeoxyribonucleases / genetics; Endodeoxyribonucleases / metabolism; HEK293 Cells; Humans; Mutation; Phylogeny; Prevotella / enzymology; Protein Biosynthesis; Protein Domains; Transcription, Genetic
TL;DR:
Cas12a nucleases therefore can exhibit widely varying properties between otherwise related orthologs, suggesting selective pressure to diversify PAM recognition and supporting expansion of the CRISPR toolbox through ortholog mining and PAM engineering.
(via Semantic Scholar)
open access via academic.oup.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 5, 2020
2020 journal article
Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems
PLOS ONE, 15(4).
MeSH headings : Animals; Drosophila / genetics; Eukaryota / genetics; Eukaryota / metabolism; Eukaryotic Cells / metabolism; Gene Expression / genetics; Gene Expression / physiology; Gene Expression Regulation / genetics; Gene Expression Regulation / physiology; Genetic Engineering / methods; Nucleic Acid Conformation; Proof of Concept Study; RNA, Catalytic / genetics; RNA, Catalytic / physiology; RNA, Messenger / metabolism; Synthetic Biology / methods
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: June 22, 2020
2019 journal article
The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs
FEMS MICROBIOLOGY LETTERS, 366(8).
author keywords: Cas nuclease; Cpf1; CRISPR-Cas systems; PAM; TIDE; TXTL
MeSH headings : Acidaminococcus / enzymology; Acidaminococcus / genetics; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; CRISPR-Associated Proteins / genetics; CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems; Catalytic Domain; DNA Cleavage; Endodeoxyribonucleases / genetics; Endodeoxyribonucleases / metabolism; Endonucleases / genetics; Endonucleases / metabolism; Escherichia coli / genetics; Gene Editing; HEK293 Cells; Humans; Nucleotide Motifs; Plasmids / genetics
TL;DR:
Results show that wild-type AsCas12a can recognize non-canonical GTTV and GCTV motifs and exemplify why the range of PAMs recognized by Cas nucleases are poorly captured with a consensus sequence.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: September 9, 2019
2018 journal article
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
MOLECULAR CELL, 69(1), 146-+.
MeSH headings : CRISPR-Cas Systems / genetics; Cell-Free System / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA, Bacterial / genetics; Endonucleases / metabolism; Escherichia coli / genetics; Genetic Engineering / methods; Oryza / genetics; Protein Biosynthesis / genetics; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic / genetics
TL;DR:
The use of E. coli cell-free transcription-translation (TXTL) systems are presented to vastly improve the speed and scalability of CRISPR characterization and validation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
A facilitated diffusion mechanism establishes the Drosophila Dorsal gradient
DEVELOPMENT, 144(23), 4450–4461.
author keywords: Morphogen; Tissue patterning; Mathematical modeling; Dorsal; Cactus; Shuttling
MeSH headings : Animals; Animals, Genetically Modified; Biophysical Phenomena; Body Patterning / genetics; Body Patterning / physiology; Computer Simulation; DNA-Binding Proteins / genetics; DNA-Binding Proteins / physiology; Drosophila Proteins / genetics; Drosophila Proteins / physiology; Drosophila melanogaster / embryology; Drosophila melanogaster / genetics; Drosophila melanogaster / physiology; Facilitated Diffusion; Female; Models, Biological; Nuclear Proteins / genetics; Nuclear Proteins / physiology; Phosphoproteins / genetics; Phosphoproteins / physiology; Signal Transduction; Toll-Like Receptors / genetics; Toll-Like Receptors / physiology; Transcription Factors / genetics; Transcription Factors / physiology
TL;DR:
Dorsal, a transcription factor that patterns the Drosophila dorsal-ventral axis, accumulates at the embryo ventral midline, and model-guided experiments show that the inhibitor Cactus shuttles Dorsal to the ventral side.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018