@article{pandolfo_cope_weaver_kwak_hoeksema_2024, title={Thermal Threats to Freshwater Mussels: An Empirical Stream Assessment}, volume={16}, ISSN={["1424-2818"]}, url={https://doi.org/10.3390/d16010037}, DOI={10.3390/d16010037}, abstractNote={Freshwater mussels fulfill an important ecological role in aquatic ecosystems, but they currently face many threats, including thermal regime alteration. Thermal transformation of the aquatic environment is associated with climate change, land use alteration, and other pervasive anthropogenic global changes. To enhance our understanding of ecological thermal impacts, we combined extensive field measurements of temperature in the stream water column and substrate depths (5 and 15 cm) at sites where mussels occur, measures of abundance and species richness for mussels and fish, and thermal tolerance knowledge for mussels and fish to generate a comprehensive assessment of the potential threats mussels face as temperatures continue to rise as a result of global change. Mean summer (June–August 2010–2012) temperatures at mussel-occupied sites in the upper Tar River basin of North Carolina, USA, ranged from 16.2 to 34.7 °C. The mean temperature from the hottest 96 h at each site ranged from 23.5 to 31.5 °C. At 80% of sites, a period of moderate drought coincided with the hottest 96 h period. Temperature threshold exceedance durations indicated that chronic, combined chronic/acute, and acute freshwater mussel thermal tolerance thresholds (i.e., 28 °C, 30 °C, and 33 °C, respectively) based on laboratory exposures of glochidia (larvae) and juveniles were commonly exceeded. Water temperatures exceeded 28 °C for at least 24 h at 55% of sites and for at least 96 h at 35% of sites, and they exceeded 30 °C for at least 24 h at 15% of sites. We quantified a thermal buffering effect of the substrate that may be protective of mussels. There was a mean difference of 0.5 °C between the water column and the upper substrate (5 cm) and a mean difference of 0.9 °C between the water column and the lower substrate (15 cm). Maximum differences of up to 5.5 °C between the water column and the upper substrate and 11.5 °C between the water column and the lower substrate were observed. Our models estimating the relation between the water column and substrate temperatures more realistically characterize ambient temperature exposures and have widespread implications for mussel conservation and climate change risk assessment in similar streams. Freshwater mussels currently exist on the edge of their thermal limits, but their abundance and species richness cannot be explained by temperature patterns alone. Fish species richness was related to the thermal regime, indicating that species interactions may be an important driver of freshwater mussel responses to global change.}, number={1}, journal={DIVERSITY-BASEL}, author={Pandolfo, Tamara J. and Cope, W. Gregory and Weaver, Daniel M. and Kwak, Thomas J. and Hoeksema, Bert W.}, year={2024}, month={Jan} }
 @article{pandolfo_kwak_cope_heise_nichols_pacifici_2016, title={Species traits and catchment-scale habitat factors influence the occurrence of freshwater mussel populations and assemblages}, volume={61}, ISSN={0046-5070}, url={http://dx.doi.org/10.1111/fwb.12807}, DOI={10.1111/fwb.12807}, abstractNote={Summary}, number={10}, journal={Freshwater Biology}, publisher={Wiley}, author={Pandolfo, Tamara J. and Kwak, Thomas J. and Cope, W. Gregory and Heise, Ryan J. and Nichols, Robert B. and Pacifici, Krishna}, year={2016}, month={Aug}, pages={1671–1684} }
 @article{daraio_bales_pandolfo_2014, title={Effects of land use and climate change on stream temperature II: Threshold exceedance duration projections for freshwater mussels}, volume={50}, DOI={10.1111/jawr.12178}, abstractNote={Abstract}, number={5}, journal={Journal of the American Water Resources Association}, author={Daraio, J. A. and Bales, J. D. and Pandolfo, T. J.}, year={2014}, pages={1177–1190} }
 @article{hazelton_cope_mosher_pandolfo_belden_barnhart_bringolf_2013, title={Fluoxetine alters adult freshwater mussel behavior and larval metamorphosis}, volume={445}, ISSN={["1879-1026"]}, DOI={10.1016/j.scitotenv.2012.12.026}, abstractNote={We used acute and partial-lifecycle tests to examine the effects of the pharmaceutical fluoxetine on freshwater mussels (Unionida). In acute tests lasting 24–48 h, we determined median effective concentrations (EC50s) for fluoxetine with larval (glochidia viability) and juvenile (survival) life-stages of fatmucket (Lampsilis siliquoidea) and black sandshell (Ligumia recta). In a 28-d behavioral test we exposed brooding adult female wavy-rayed lampmussels (Lampsilis fasciola) to 0.37 and 29.3 μg/L fluoxetine to determine effects on adult behavior (foot protrusion, mantle lure display and glochidia parturition). We also assessed the effects of 24-h exposure of 1 and 100 μg/L fluoxetine on glochidia viability duration and metamorphosis success for the wavy-rayed lampmussel. Fluoxetine EC50s ranged from 62 μg/L for juveniles (96 h) to 293 μg/L for glochidia (24 h). In adults, statistically significant increases were observed in foot protrusion at 0.37 and 29.3 μg/L fluoxetine and lure display rates at 29.3 μg/L; glochidia parturition was not significantly affected at any test concentration. Twenty-four hour exposure of glochidia to fluoxetine did not affect viability duration, but likelihood of metamorphosis to the juvenile stage significantly increased with 1 and 100 μg/L treatments. Our results demonstrated effects of fluoxetine to unionid mussels at concentrations less than previously reported and approaching concentrations measured in surface waters.}, journal={SCIENCE OF THE TOTAL ENVIRONMENT}, author={Hazelton, Peter D. and Cope, W. Gregory and Mosher, Shad and Pandolfo, Tamara J. and Belden, Jason B. and Barnhart, M. Christopher and Bringolf, Robert B.}, year={2013}, month={Feb}, pages={94–100} }
 @article{pandolfo_cope_young_jones_hua_lingenfelser_2012, title={Acute effects of road salts and associated cyanide compounds on the early life stages of the unionid mussel Villosa iris}, volume={31}, ISSN={["0730-7268"]}, DOI={10.1002/etc.1876}, abstractNote={Abstract}, number={8}, journal={ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY}, author={Pandolfo, Tamara J. and Cope, W. Gregory and Young, George B. and Jones, Jess W. and Hua, Dan and Lingenfelser, Susan F.}, year={2012}, month={Aug}, pages={1801–1806} }
 @article{hazelton_cope_pandolfo_mosher_strynar_barnhart_bringolf_2012, title={Partial life-cycle and acute toxicity of perfluoroalkyl acids to freshwater mussels}, volume={31}, ISSN={["1552-8618"]}, DOI={10.1002/etc.1866}, abstractNote={Abstract}, number={7}, journal={ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY}, author={Hazelton, Peter D. and Cope, W. Gregory and Pandolfo, Tamara J. and Mosher, Shad and Strynar, Mark J. and Barnhart, M. Christopher and Bringolf, Robert B.}, year={2012}, month={Jul}, pages={1611–1620} }
 @article{pandolfo_cope_arellano_2010, title={THERMAL TOLERANCE OF JUVENILE FRESHWATER MUSSELS (UNIONIDAE) UNDER THE ADDED STRESS OF COPPER}, volume={29}, ISSN={["1552-8618"]}, DOI={10.1002/etc.92}, abstractNote={Abstract}, number={3}, journal={ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY}, author={Pandolfo, Tamara J. and Cope, W. Gregory and Arellano, Consuelo}, year={2010}, month={Mar}, pages={691–699} }
 @article{pandolfo_cope_arellano_bringolf_barnhart_hammer_2010, title={Upper thermal tolerances of early life stages of freshwater mussels}, volume={29}, ISSN={["0887-3593"]}, DOI={10.1899/09-128.1}, abstractNote={Abstract Freshwater mussels (order Unioniformes) fulfill an essential role in benthic aquatic communities, but also are among the most sensitive and rapidly declining faunal groups in North America. Rising water temperatures, caused by global climate change, industrial discharges, drought, or land development, could further challenge imperiled unionid communities. The aim of our study was to determine the upper thermal tolerances of the larval (glochidia) and juvenile life stages of freshwater mussels. Glochidia of 8 species of mussels were tested: Lampsilis siliquoidea, Potamilus alatus, Ligumia recta, Ellipsaria lineolata, Lasmigona complanata, Megalonaias nervosa, Alasmidonta varicosa, and Villosa delumbis. Seven of these species also were tested as juveniles. Survival trends were monitored while mussels held at 3 acclimation temperatures (17, 22, and 27°C) were exposed to a range of common and extreme water temperatures (20–42°C) in standard acute laboratory tests. The average median lethal temperature (LT50) among species in 24-h tests with glochidia was 31.6°C and ranged from 21.4 to 42.7°C. The mean LT50 in 96-h juvenile tests was 34.7°C and ranged from 32.5 to 38.8°C. Based on comparisons of LT50s, thermal tolerances differed among species for glochidia, but not for juveniles. Acclimation temperature did not affect thermal tolerance for either life stage. Our results indicate that freshwater mussels already might be living close to their upper thermal tolerances in some systems and, thus, might be at risk from rising environmental temperatures.}, number={3}, journal={JOURNAL OF THE NORTH AMERICAN BENTHOLOGICAL SOCIETY}, author={Pandolfo, Tamara J. and Cope, W. Gregory and Arellano, Consuelo and Bringolf, Robert B. and Barnhart, M. Christopher and Hammer, Edward}, year={2010}, month={Sep}, pages={959–969} }
 @article{pandolfo_cope_arellano_2009, title={Heart rate as a sublethal indicator of thermal stress in juvenile freshwater mussels}, volume={154}, ISSN={["1531-4332"]}, DOI={10.1016/j.cbpa.2009.07.001}, abstractNote={Freshwater mussels (Unionoida) are one of the most sensitive and rapidly declining faunal groups in the world. Rising water temperatures, caused by industrial discharges, land development, or climate change can further challenge threatened unionid communities. The direct relationship between heart rate and temperature in ectotherms enables the use of heart rate as an indicator of whole-animal thermal stress. The purpose of this study was to assess the utility of heart rate as an indicator of thermal stress in freshwater mussels. Seven species of juvenile mussels (Lampsilis siliquoidea, Potamilus alatus, Ligumia recta, Ellipsaria lineolata, Megalonaias nervosa, Alasmidonta varicosa, and Villosa delumbis) were evaluated in response to a range of experimental temperatures (20–36 °C) at three acclimation temperatures (17, 22, and 27 °C). Heart rate was measured by direct visual observation through transparent mussel shells. The average heart rate for all 7 species at 20 °C was 55 bpm, with a range from 38 bpm (L. recta) to 65 bpm (P. alatus). L. recta and V. delumbis exhibited significant changes in heart rate with increasing temperature at each of the three acclimation temperatures. The use of heart rate appears to be a suitable indicator of thermal stress in some unionid mussels.}, number={3}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY}, author={Pandolfo, Tamara J. and Cope, W. Gregory and Arellano, Consuelo}, year={2009}, month={Nov}, pages={347–352} }