Works (65)

Updated: July 22nd, 2024 08:05

2024 journal article

Multi-amplicon nitrogen cycling gene standard: An innovative approach for quantifying N-transforming soil microbes in terrestrial ecosystems

Soil Biology and Biochemistry.

By: C. Gillespie n, T. Sit n, A. Loucanides n, J. Hu n, B. Matusko n, N. Terwilliger n, W. Shi n, S. Steffen n, A. Woodley n, S. Hu n

Source: ORCID
Added: May 15, 2024

2023 journal article

Cellulose Acetate-Stabilized Pickering Emulsions: Preparation, Rheology, and Incorporation of Agricultural Active Ingredients

ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 11(42), 15178–15191.

By: M. Sohail n, T. Pirzada n, R. Guenther n, E. Barbieri n, T. Sit n, S. Menegatti n, N. Crook n, C. Opperman n, S. Khan n

author keywords: nonsporulating microbes; double yielding; Pseudomonas simiae; microstructure; controlledrelease
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 1, 2023

2023 journal article

D'Ann Rochon (1955-2022), a life of passion for plant virology

VIROLOGY, 587.

By: H. Sanfacon*, S. Alam*, B. Ghoshal*, K. Ghoshal*, E. Hui*, A. Jackson*, K. Kakani*, T. Morris* ...

author keywords: D'Ann Rochon; Tribute; Plant virology; Tombusviruses; Fungus transmission; Virus structure; Plum pox virus; Little cherry virus
TL;DR: Her research program provided new insights on the infection cycle of tombusviruses and related viruses, including ground-breaking research on the structure of virus particles, the mechanisms of virus transmission by fungal zoospores, and the complexity of plant-virus interactions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 23, 2023

2023 journal article

Plant-biomass-based hybrid seed wraps mitigate yield and post-harvest losses among smallholder farmers in sub-Saharan Africa

Nature Food, 4(2), 148–159.

By: T. Pirzada n, A. Affokpon*, R. Guenther n, R. Mathew n, S. Agate n, A. Blevins n, M. Byrd n, T. Sit n ...

MeSH headings : Humans; Benin; Biomass; Farmers; Plant Tubers; Seeds; Agriculture / methods; Crop Protection
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: February 17, 2023

2022 journal article

Characterization and Aggressiveness of Take-All Root Rot Pathogens Isolated from Symptomatic Bermudagrass Putting Greens

PHYTOPATHOLOGY, 112(4), 811–819.

By: C. Stephens n, T. Gannon n, M. Cubeta n, T. Sit n & J. Kerns n

author keywords: disease control and pest management; epidemiology; etiology; fungal pathogens; pathogen detection; techniques
MeSH headings : Ascomycota; Cynodon / microbiology; Plant Diseases / microbiology
TL;DR: Information is provided on this disease and its causal agents that may improve take-all root rot management and improving pathogen identification and understanding of the aggressiveness of these pathogens along with growth at different temperatures will advance the knowledge of disease development to optimize management strategies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 2, 2022

2022 journal article

Comprehensive Perception Approach of Adoption: Innovative Wrap & Plant Technology for Nematodes Management on Yam

Advances in Social Sciences Research Journal, 9(4), 355–368.

By: H. Dedehouanou* & A. Affokpon*

Source: ORCID
Added: June 27, 2023

2022 journal article

Wrap & Plant Plant Technology: An Innovative and Cost-Effective Method for Seed Yam Treatment for Nematode Control in Fields

Wrap & Plant Plant Technology: An Innovative and Cost-Effective Method for Seed Yam Treatment for Nematode Control in Fields. Advances in Social Sciences Research Journal, 9(5), 39–59.

By: H. Dedehouanou*

Source: ORCID
Added: June 27, 2023

2022 article

Wrap-and-plant technology to manage sustainably potato cyst nematodes in East Africa

Ochola, J., Cortada, L., Mwaura, O., Tariku, M., Christensen, S. A., Ng'ang'a, M., … Torto, B. (2022, February 28). NATURE SUSTAINABILITY, Vol. 2.

By: J. Ochola*, L. Cortada*, O. Mwaura*, M. Tariku*, S. Christensen*, M. Ng'ang'a*, A. Hassanali*, T. Pirzada n ...

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 1, 2022

2022 article

Wrap-and-plant technology to manage sustainably potato cyst nematodes in East Africa

(2022, February 28). OpenAlex.

Contributors: J. Ochola, L. Cortada, O. Mwaura, M. Chernet, S. Christensen, M. Ng'ang'a, A. Hassanali, T. Pirzada ...

Source: ORCID
Added: July 1, 2024

2022 article

Wrap-and-plant technology to manage sustainably potato cyst nematodes in East Africa

(2022, February 28). OpenAlex.

Contributors: J. Ochola, L. Cortada, O. Mwaura, M. Chernet, S. Christensen, M. Ng'ang'a, A. Hassanali, T. Pirzada ...

Source: ORCID
Added: July 1, 2024

2021 journal article

Detection of Fruit Meals Within Laboratory-Raised and Field-Trapped Adult Drosophila suzukii (Diptera: Drosophilidae) Guts

Frontiers in Ecology and Evolution, 9.

author keywords: spotted wing Drosophila; integrated pest management; invasive species; gut content; small fruit IPM
TL;DR: Molecular methods to study adult D. suzukii gut content suggest that adult flies move between crop and non-crop habitats and may not be highly dependent on fruit food resources. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: September 20, 2021

2021 journal article

PAH-pollution effects on sensitive and resistant embryos: Integrating structure and function with gene expression

PLOS ONE, 16(4).

MeSH headings : Adaptation, Physiological / drug effects; Animals; Embryo, Nonmammalian / drug effects; Embryo, Nonmammalian / metabolism; Embryo, Nonmammalian / physiology; Gene Expression Regulation, Developmental / drug effects; Polycyclic Aromatic Hydrocarbons / chemistry; Polycyclic Aromatic Hydrocarbons / toxicity; Water Pollutants, Chemical / toxicity
TL;DR: Gene expression analysis of early somitogenesis, heartbeat initiation, late organogenesis, and pre-hatching developmental stages reveals genes whose expression significantly differs between sensitive and resistant embryo populations and helps to explain mechanisms of sensitivity and resistance to polluted environments during vertebrate animal development. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 10, 2021

2020 journal article

A CRISPR/dCas9 toolkit for functional analysis of maize genes

Plant Methods, 16(1).

By: I. Gentzel*, C. Park*, M. Bellizzi*, G. Xiao*, K. Gadhave n, C. Murphree n, Q. Yang n, J. LaMantia* ...

Contributors: I. Gentzel*, C. Park*, M. Bellizzi*, G. Xiao*, K. Gadhave n, C. Murphree n, Q. Yang n, J. Lamantia* ...

author keywords: Maize; Protoplasts; CRISPR; Cas9; Transcription activation; Transcription suppression
TL;DR: This study describes a vector toolkit for analyzing dCas9-mediated activation (CRISPRa) or inactivation ( CRISPRi) of gene expression in maize protoplasts and expects that this maize protoplast toolkit will streamline the analysis of gRNA candidates and facilitate genetic studies of important trait genes in this transformation-recalcitrant plant. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: October 26, 2020

2020 journal article

Localization of viral and host RNA within soybean cyst nematode via fluorescence in situ hybridization

EXPERIMENTAL PARASITOLOGY, 211.

By: C. Ruark-Seward n, E. Davis n & T. Sit n

Contributors: C. Ruark-Seward n, E. Davis n & T. Sit n

TL;DR: Three viruses within the soybean cyst nematode are localized: SCN socyvirus-1 (SbCNV-1), SCN nyami-like virus (NLV), and SCN bunya- like virus (BLV) through whole-mount fluorescence in situ hybridization (FISH) methodology, highlighting the specificity of the FISH method. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: April 20, 2020

2020 journal article

Near-Atomic-Resolution Cryo-Electron Microscopy Structures of Cucumber Leaf Spot Virus and Red Clover Necrotic Mosaic Virus: Evolutionary Divergence at the Icosahedral Three-Fold Axes

JOURNAL OF VIROLOGY, 94(2).

By: M. Sherman*, R. Guenther n, R. Reade*, D. Rochon*, T. Sit n & T. Smith*

Contributors: M. Sherman*, R. Guenther n, R. Reade*, R. D’Ann*, T. Sit n & T. Smitha

Ed(s): C. Parrish

author keywords: Tombusviridae; electron microscopy; plant viruses; virion structure
MeSH headings : Capsid / ultrastructure; Capsid Proteins / ultrastructure; Cryoelectron Microscopy; Evolution, Molecular; Tobacco; Tombusviridae / ultrastructure
TL;DR: It appears that the ssRNA binding (R) domain of the capsid diverged evolutionarily in order to recognize the different genomes, and the R and arm domains may have coevolved to package different genomes within the conserved and rigid shell. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 21, 2020

2020 journal article

Quantitative Real-Time PCR Analysis of Individual Flue-Cured Tobacco Seeds and Seedlings Reveals Seed Transmission of Tobacco Mosaic Virus

PHYTOPATHOLOGY, 110(1), 194–205.

By: M. Ellis*, J. Hoak*, B. Ellis*, J. Brown n, T. Sit n, C. Wilkinson*, T. Reed*, G. Welbaum*

Contributors: M. Ellis*, J. Hoak*, B. Ellis*, J. Brown n, T. Sit n, C. Wilkinson*, T. Reed*, G. Welbaum*

author keywords: RT-qPCR; seedborne transmission; seed transmission; Tobacco mosaic virus; virology
MeSH headings : Plant Diseases / virology; Real-Time Polymerase Chain Reaction; Seedlings / virology; Seeds / virology; Tobacco / virology; Tobacco Mosaic Virus / genetics; Tobacco Mosaic Virus / physiology
TL;DR: To the authors' knowledge, this is the first report of TMV being detected in embryos of tobacco seeds, demonstrating that TMV is seedborne and seed-transmitted in flue-cured tobacco. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: February 17, 2020

2020 journal article

Recent Advances in Biodegradable Matrices for Active Ingredient Release in Crop Protection: Towards Attaining Sustainability in Agriculture

Current Opinion in Colloid & Interface Science, 48, 121–136.

By: T. Pirzada n, B. Farias n, R. Mathew n, R. Guenther n, M. Byrd n, T. Sit n, L. Pal n, C. Opperman n, S. Khan n

Contributors: T. Pirzada n, B. Farias n, R. Mathew n, R. Guenther n, M. Byrd n, T. Sit n, L. Pal n, C. Opperman n, S. Khan n

author keywords: Biodegradable matrix; Crop protection; Active ingredient release; Agriculture; Formulation; Seed coating; Encapsulation; Electrospinning; Nanofibers
TL;DR: Some of the recent approaches to use biodegradable matrices in crop protection, while exploring their design and efficiency are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: ORCID
Added: May 29, 2020

2020 journal article

Relationships of virus titers and transmission rates among sympatric and allopatric virus isolates and thrips vectors support local adaptation

Scientific Reports, 10(1).

By: J. Linak n, A. Jacobson*, T. Sit n & G. Kennedy n

Contributors: J. Linak n, A. Jacobson*, T. Sit n & G. Kennedy n

MeSH headings : Animals; Insect Vectors / virology; Plant Diseases / virology; Plant Viruses / physiology; Tospovirus; Viral Load
TL;DR: It is demonstrated that although transmission rates were higher for sympatric than allopatric TSWV isolate-T. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: June 8, 2020

2020 journal article

Tailored Lignocellulose-Based Biodegradable Matrices with Effective Cargo Delivery for Crop Protection

ACS Sustainable Chemistry & Engineering, 8(17), 6590–6600.

By: T. Pirzada n, R. Mathew n, R. Guenther n, T. Sit n, C. Opperman n, L. Pal n, S. Khan n

Contributors: T. Pirzada n, R. Mathew n, R. Guenther n, T. Sit n, C. Opperman n, L. Pal n, S. Khan n

author keywords: Banana paper; Sustainable agriculture; Pulp refining Abamectin; Wrap and plant
TL;DR: An innovative and cost-effective approach to fabricate lignocellulose-based biodegradable porous matrices capable of slow and sustained release of the loaded molecules for effective crop protection. (via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: April 1, 2020

2019 journal article

Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens

METHODSX, 6, 2720–2728.

By: C. Ruark-Seward n, E. Davis n & T. Sit n

Contributors: C. Ruark-Seward n, E. Davis n & T. Sit n

author keywords: Nematode; RNA; FISH; Localization; Fluorescence; Gene expression; Virus
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 9, 2019

2019 journal article

Electrospun Polymer Nanofibers as Seed Coatings for Crop Protection

ACS Sustainable Chemistry & Engineering, 7(24), 19848–19856.

By: B. Farias n, T. Pirzada n, R. Mathew n, T. Sit n, C. Opperman n & S. Khan n

Contributors: B. Farias n, T. Pirzada n, R. Mathew n, T. Sit n, C. Opperman n & S. Khan n

author keywords: electrospinning; seed coating; cellulose; fluopyram; abamectin; controlled release
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: ORCID
Added: November 26, 2019

2018 journal article

A folded viral noncoding RNA blocks host cell exoribonucleases through a conformationally dynamic RNA structure

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115(25), 6404–6409.

By: A. Steckelberg*, B. Akiyama*, D. Costantino*, T. Sit n, J. Nix* & J. Kieft*

Contributors: A. Steckelberg*, B. Akiyama*, D. Costantino*, T. Sit n, J. Nix* & J. Kieft*

author keywords: noncoding RNA maturation; RNA structure; RNA dynamics; single-molecule FRET; exoribonuclease resistance
MeSH headings : 3' Untranslated Regions / genetics; Animals; Base Sequence; Exoribonucleases / metabolism; Flavivirus / genetics; Host-Pathogen Interactions / genetics; Nucleic Acid Conformation; RNA Folding / genetics; RNA Stability / genetics; RNA, Viral / genetics
TL;DR: The results show that xrRNAs are an authentic class of functional RNAs that have arisen independently in different contexts, suggesting that they may be widespread and establishes RNA structure-dependent exoribonuclease resistance inDifferent contexts as a general RNA maturation mechanism and defines xr RNAs as an authentic functional class of RNAs. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2018 journal article

Detecting Specific Resource Use by Drosophila suzukii (Diptera: Drosophilidae) Using Gut Content Analysis

JOURNAL OF ECONOMIC ENTOMOLOGY, 111(3), 1496–1500.

Contributors: L. Diepenbrock n, J. Lundgren*, T. Sit n & H. Burrack n

author keywords: spotted wing drosophila; gut content; plant pest
MeSH headings : Animals; DNA, Plant / analysis; Diet; Drosophila / physiology; Feeding Behavior; Female; Food Chain; Fragaria / chemistry; Fruit / chemistry; Gastrointestinal Tract; Polymerase Chain Reaction / methods
TL;DR: The capability of polymerase chain reaction-based gut content analysis to detect a known food resource from DNA extracted from laboratory-reared flies is evaluated and it is validated that DNA from this host could be detected for up to 7 d post-consumption. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2018 journal article

Novel RNA viruses within plant parasitic cyst nematodes

PLOS ONE, 13(3).

By: C. Ruark n, M. Gardner*, M. Mitchum*, E. Davis n & T. Sit n

Contributors: C. Ruark n, M. Gardner*, M. Mitchum*, E. Davis n & T. Sit n

Ed(s): U. Melcher

MeSH headings : Animals; Beta vulgaris / parasitology; Gene Expression Profiling; Genome, Viral / genetics; Medicago / parasitology; Nematoda / virology; Plants / parasitology; RNA Viruses / genetics; Real-Time Polymerase Chain Reaction; Solanum tuberosum / parasitology; Soybeans / parasitology
TL;DR: Five single-stranded RNA viruses have now been confirmed within the economically important soybean cyst nematode (SCN; Heterodera glycines), and two previously undiscovered viral-like genomes are identified and named. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2018 article

Plant Virus-Based Nanoparticles for the Delivery of Agronomic Compounds as a Suspension Concentrate

VIRUS-DERIVED NANOPARTICLES FOR ADVANCED TECHNOLOGIES: METHODS AND PROTOCOLS, Vol. 1776, pp. 203–214.

By: R. Guenther n, S. Lommel n, C. Opperman n & T. Sit n

Contributors: R. Guenther n, S. Lommel n, C. Opperman n & T. Sit n

author keywords: Plant pathogenic nematode; Nematicide; Agrichemical; Plant viral nanoparticle; Nanoparticle formulation
MeSH headings : Ivermectin / analogs & derivatives; Ivermectin / chemistry; Nanoparticles / chemistry; Plant Viruses / chemistry; Tombusviridae / chemistry
TL;DR: This work describes how to formulate a PVN loaded with the nematicide abamectin (Abm) beginning with the propagation of the virus through the formulation, deactivation, and characterization of the finished product. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 14, 2019

2017 journal article

Soybean cyst nematode culture collections and field populations from North Carolina and Missouri reveal high incidences of infection by viruses

PLOS ONE, 12(1).

Contributors: C. Ruark n, S. Koenning n, E. Davis n, C. Opperman n, S. Lommel n, M. Mitchum*, T. Sit n

Ed(s): A. Rao

MeSH headings : Animals; DNA-Directed RNA Polymerases / metabolism; Gene Expression Regulation, Plant; Incidence; Life Cycle Stages / genetics; Missouri; North Carolina; Plant Diseases / parasitology; Plant Diseases / statistics & numerical data; Plant Diseases / virology; Plant Viruses / physiology; Real-Time Polymerase Chain Reaction; Soybeans / genetics; Soybeans / parasitology; Soybeans / virology; Species Specificity; Tylenchoidea / growth & development; Tylenchoidea / physiology; Virus Replication / physiology
TL;DR: Viral titers within SCN greenhouse cultures were similar throughout juvenile development, and the presence of viral anti-genomic RNAs within egg, second-stage juvenile (J2), and pooled J4 stages suggests active viral replication within the nematode. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 journal article

Development of abamectin loaded lignocellulosic matrices for the controlled release of nematicide for crop protection

CELLULOSE, 23(1), 673–687.

By: J. Cao n, R. Guenther n, T. Sit n, S. Lommel n, C. Opperman n & J. Willoughby n

Contributors: J. Cao n, R. Guenther n, T. Sit n, S. Lommel n, C. Opperman n & J. Willoughby n

author keywords: Lignocellulosic matrix; Controlled release; Abamectin; Lignin; Crop protection
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 journal article

Development of Abamectin Loaded Plant Virus Nanoparticles for Efficacious Plant Parasitic Nematode Control

ACS Applied Materials & Interfaces, 7(18), 9546–9553.

By: J. Cao n, R. Guenther n, T. Sit n, S. Lommel n, C. Opperman n & J. Willoughby n

Contributors: J. Cao n, R. Guenther n, T. Sit n, S. Lommel n, C. Opperman n & J. Willoughby n

author keywords: red clover necrotic mosaic virus (RCNMV); plant virus nanoparticle (PVN); abamectin; soil mobility; crop protection
MeSH headings : Animals; Biological Availability; Caenorhabditis elegans / drug effects; Capsid / chemistry; Crops, Agricultural / drug effects; Crops, Agricultural / parasitology; Ivermectin / analogs & derivatives; Ivermectin / pharmacology; Solanum lycopersicum / drug effects; Solanum lycopersicum / parasitology; Nanoparticles / chemistry; Nematoda / drug effects; Pest Control, Biological; Plant Diseases / parasitology; Plant Viruses / chemistry; Soil; Suspensions; Tobacco / drug effects; Tobacco / parasitology; Tylenchoidea / drug effects
TL;DR: Tomato seedlings treated with PVN(Abm) had healthier root growth and a reduction in root galling demonstrating the success of this delivery system for the increased efficacy of Abm to control nematode damage in crops. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science, Crossref
Added: August 6, 2018

2015 article

Tombusviridae

Sit, T. L., & Lommel, S. A. (2015, November). ELS.

By: T. Sit n & S. Lommel n

Source: ORCID
Added: July 21, 2020

2014 journal article

Loading and Release Mechanism of Red Clover Necrotic Mosaic Virus Derived Plant Viral Nanoparticles for Drug Delivery of Doxorubicin

SMALL, 10(24), 5126–5136.

By: J. Cao n, R. Guenther n, T. Sit n, C. Opperman n, S. Lommel n & J. Willoughby n

Contributors: J. Cao n, R. Guenther n, T. Sit n, C. Opperman n, S. Lommel n & J. Willoughby n

MeSH headings : Antibiotics, Antineoplastic / administration & dosage; Antibiotics, Antineoplastic / pharmacokinetics; Capsid; Doxorubicin / administration & dosage; Doxorubicin / pharmacokinetics; Drug Carriers; Hydrogen-Ion Concentration; Nanoparticles; Tombusviridae / chemistry
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 journal article

Genomic and physiological responses to strong selective pressure during late organogenesis: few gene expression changes found despite striking morphological differences

BMC Genomics, 14(1), 779.

By: G. Bozinovic n, T. Sit n, R. Giulio*, L. Wills* & M. Oleksiak n

Contributors: G. Bozinovic n, T. Sit n, R. Di Giulio*, L. Wills* & M. Oleksiak n

author keywords: Development; Genomics; Embryos; Adaptation; Evolution; Fish; Transcriptomics
MeSH headings : Animals; Benzoflavones / toxicity; Cytochrome P-450 CYP1A1 / antagonists & inhibitors; Cytochrome P-450 CYP1A1 / genetics; Embryo, Nonmammalian; Embryonic Development / drug effects; Embryonic Development / genetics; Environmental Pollution; Fundulidae / embryology; Fundulidae / genetics; Gene Expression Regulation, Developmental; Organogenesis / drug effects; Organogenesis / genetics; Receptors, Aryl Hydrocarbon / agonists; Selection, Genetic / drug effects; Selection, Genetic / genetics; beta-Naphthoflavone / toxicity
TL;DR: It is suggested that statistically significant changes in gene expression of relatively few genes contribute to the phenotypic changes and large morphological differences exhibited by reference and resistant populations upon exposure to PAH pollutants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: ORCID
Added: July 21, 2020

2013 journal article

New insight into the structure of RNA in red clover necrotic mosaic virus and the role of divalent cations revealed by small-angle neutron scattering

ARCHIVES OF VIROLOGY, 158(8), 1661–1669.

By: S. Martin n, L. He*, F. Meilleur*, R. Guenther n, T. Sit n, S. Lommel n, W. Heller*

Contributors: S. Martin n, L. He*, F. Meilleur*, R. Guenther n, T. Sit n, S. Lommel n, W. Heller*

TL;DR: Small-angle neutron scattering with contrast variation experiments were performed to better understand the organization of the RNA and conformational changes resulting from the removal of divalent cations in red clover necrotic mosaic virus and provide new insight into changes in the RNA packing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 journal article

The red clover necrotic mosaic virus capsid protein N-terminal amino acids possess specific RNA binding activity and are required for stable virion assembly

VIRUS RESEARCH, 176(1-2), 107–118.

By: S. Park*, T. Sit n, K. Kim n & S. Lommel n

Contributors: S. Park*, T. Sit n, K. Kim n & S. Lommel n

author keywords: RCNMV; Virus assembly; RNA-CP interactions; TABS; TA; Co-packaging
MeSH headings : Capsid Proteins / genetics; Capsid Proteins / metabolism; DNA Mutational Analysis; RNA-Binding Proteins / genetics; RNA-Binding Proteins / metabolism; Sequence Deletion; Tombusviridae / genetics; Tombusviridae / physiology; Virion / metabolism; Virus Assembly
TL;DR: The N-terminal region of the CP is involved in both producing two virion populations due to its RNA binding properties and virion stability and was indispensable for stable virion formation and the region spanning CP residues 5-15 is required for systemic movement. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

The Red clover necrotic mosaic virus capsid protein N-terminal lysine-rich motif is a determinant of symptomatology and virion accumulation

MOLECULAR PLANT PATHOLOGY, 13(7), 744–754.

By: S. Park*, T. Sit n, K. Kim n & S. Lommel n

Contributors: S. Park*, T. Sit n, K. Kim n & S. Lommel n

MeSH headings : Alanine / genetics; Amino Acid Motifs; Amino Acid Sequence; Amino Acid Substitution / genetics; Capsid Proteins / chemistry; Capsid Proteins / metabolism; Genome, Viral / genetics; Lysine / metabolism; Molecular Sequence Data; Mutation / genetics; Plant Diseases / virology; Plant Viral Movement Proteins / metabolism; RNA, Viral / metabolism; Sequence Alignment; Structure-Activity Relationship; Tobacco / virology; Tombusviridae / genetics; Tombusviridae / metabolism; Tombusviridae / physiology; Tombusviridae / ultrastructure; Virion / metabolism; Virion / ultrastructure
TL;DR: Observations reaffirm the necessity of the N-terminal lysine-rich residues of the RCNMV CP for efficient virion accumulation and reveal additional roles for the CP in the modulation of host symptomatology, independent of its role in virion assembly and the rate of systemic viral movement in N. benthamiana. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2011 article

Dianthovirus

The Springer Index of Viruses, pp. 1895–1900.

By: T. Sit n & S. Lommel n

Source: ORCID
Added: July 21, 2020

2011 journal article

Gene expression throughout a vertebrate's embryogenesis

BMC GENOMICS, 12(1).

By: G. Bozinovic n, T. Sit n, D. Hinton* & M. Oleksiak*

Contributors: G. Bozinovic n, T. Sit n, D. Hinton* & M. Oleksiak*

MeSH headings : Animals; Cluster Analysis; Embryonic Development / genetics; Fundulidae / embryology; Fundulidae / genetics; Gene Expression Profiling; Gene Expression Regulation, Developmental; Models, Statistical; Oligonucleotide Array Sequence Analysis; Ribosomes / genetics; Sequence Analysis, RNA
TL;DR: Statistical support for the temporal dynamics of developmental gene expression during all stages of vertebrate development is provided using four biological replicates per stage in the teleost Fundulus heteroclitus. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2010 journal article

Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 66(11), 1458–1462.

By: S. Martin n, R. Guenther n, T. Sit n, P. Swartz n, F. Meilleur n, S. Lommel n, R. Rose n, F. Section

Contributors: S. Martin n, R. Guenther n, T. Sit n, P. Swartz n, F. Meilleur n, S. Lommel n, R. Rose n

author keywords: red clover necrotic mosaic virus; dianthoviruses; viruses
MeSH headings : Crystallization; Crystallography, X-Ray; Microscopy, Electron, Transmission; Tombusviridae / chemistry; Tombusviridae / ultrastructure; Virion / chemistry; Virion / ultrastructure
TL;DR: Red clover necrotic mosaic virus is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid and crystals diffracted to better than 4 Å resolution but were very radiation-sensitive, causing rapid decay of the high-resolution reflections. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 article

Tombusviridae

Sit, T. L., & Lommel, S. A. (2010, April). Encyclopedia of Life Sciences.

By: T. Sit* & S. Lommel

Source: ORCID
Added: July 21, 2020

2009 journal article

The Red clover necrotic mosaic virus origin of assembly is delimited to the RNA-2 trans-activator

VIROLOGY, 384(1), 169–178.

By: V. Basnayake n, T. Sit n & S. Lommel n

Contributors: V. Basnayake n, T. Sit n & S. Lommel n

author keywords: Red clover necrotic mosaic virus; Virion assembly; Origin of assembly; trans-activator; Dianthovirus; Tombusviridae
MeSH headings : Base Pairing; Capsid Proteins / genetics; Genome, Viral; Open Reading Frames; Promoter Regions, Genetic; RNA, Viral / genetics; Tombusviridae / genetics; Tombusviridae / physiology; Tombusvirus / genetics; Trans-Activators / genetics; Transcription, Genetic; Trifolium / virology; Viral Proteins / genetics; Virion / genetics; Virion / physiology; Virus Replication
TL;DR: It is hypothesize that RNA-1 must be base-paired with RNA-2 at the TA to initiate co-packaging, and illustrates the critical importance of the multifunctional TA element as a key regulatory switch in the RCNMV life cycle. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2008 journal article

A versatile assay for the identification of RNA silencing suppressors based on complementation of viral movement

MOLECULAR PLANT-MICROBE INTERACTIONS, 21(7), 879–890.

By: J. Powers n, T. Sit n, F. Qu*, T. Morris*, K. Kim* & S. Lommel n

Contributors: J. Powers n, T. Sit n, F. Qu*, T. Morris*, K. Kim* & S. Lommel n

author keywords: IVIS lumina; p8; p9; pPZP212; VSR
MeSH headings : Agrobacterium tumefaciens / genetics; Arabidopsis / genetics; Arabidopsis / virology; Base Sequence; Capsid Proteins / genetics; Capsid Proteins / physiology; Carmovirus / genetics; Carmovirus / pathogenicity; Carmovirus / physiology; DNA, Viral / genetics; Genes, Reporter; Genetic Complementation Test; Green Fluorescent Proteins / genetics; Host-Pathogen Interactions / genetics; Movement; Plant Diseases / genetics; Plant Diseases / virology; RNA Interference; RNA, Viral / genetics; Recombinant Proteins / genetics; Suppression, Genetic; Tobacco / genetics; Tobacco / virology
TL;DR: While the TCV-sGFP construct is primarily used as an infectious RNA transcript, it was also subcloned for direct expression from Agrobacterium tumefaciens for simple quantification of suppressor activity based on fluorescence levels in whole leaves, providing the flexibility to assay for suppressorActivity in either the cytoplasm or nucleus, depending on the construct employed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2008 journal article

The Red clover necrotic mosaic virus RNA-2 encoded movement protein is a second suppressor of RNA silencing

VIROLOGY, 381(2), 277–286.

By: J. Powers n, T. Sit n, C. Heinsohn n, C. George n, K. Kim* & S. Lommel n

Contributors: J. Powers n, T. Sit n, C. Heinsohn n, C. George n, K. Kim* & S. Lommel n

author keywords: TCV-sGFP; Red clover necrotic mosaic virus; RCNMV; Movement protein; MP; VSR; RNA silencing
MeSH headings : Carmovirus / genetics; Carmovirus / metabolism; Genetic Complementation Test; Green Fluorescent Proteins / metabolism; Plant Viral Movement Proteins / metabolism; RNA Interference; RNA, Viral / genetics; RNA, Viral / metabolism; Tombusviridae / genetics; Tombusviridae / metabolism
TL;DR: A newly developed viral-based assay for the identification of silencing suppression activity was used to provide evidence for a second, mechanistically distinct method ofsilencing suppression provided for by the RCNMV movement protein (MP). (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2008 article

Tombusviruses

Encyclopedia of Virology, pp. 145–151.

By: S. Lommel & T. Sit*

Contributors: S. Lommel & T. Sit*

Source: ORCID
Added: July 21, 2020

2008 journal article

Unique transcription start sites and distinct promoter regions differentiate the pregnane X receptor (PXR) isoforms PXR 1 and PXR 2

DRUG METABOLISM AND DISPOSITION, 36(5), 923–929.

By: L. Tompkins*, T. Sit* & A. Wallace*

Contributors: L. Tompkins*, T. Sit* & A. Wallace*

MeSH headings : Cell Line, Tumor; Cytochrome P-450 CYP3A / genetics; Gene Expression; Humans; Liver / metabolism; Pregnane X Receptor; Promoter Regions, Genetic; Protein Isoforms / genetics; RNA, Messenger / metabolism; Receptors, Cytoplasmic and Nuclear; Receptors, Steroid / genetics; Reverse Transcriptase Polymerase Chain Reaction; Transcription Initiation Site; Transfection
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2006 journal article

Proximal cavity, distal histidine, and substrate hydrogen-bonding mutations modulate the activity of Amphitrite ornata dehaloperoxidase

BIOCHEMISTRY, 45(30), 9085–9094.

By: S. Franzen n, J. Belyea n, L. Gilvey n, M. Davis n, C. Chaudhary n, T. Sit n, S. Lommel n

Contributors: S. Franzen n, J. Belyea n, L. Gilvey n, M. Davis n, C. Chaudhary n, T. Sit n, S. Lommel n

MeSH headings : Animals; Binding Sites / genetics; Hemoglobins; Histidine / chemistry; Histidine / genetics; Hydrogen Bonding; Mutagenesis, Site-Directed; Peroxidases / chemistry; Peroxidases / genetics; Peroxidases / metabolism; Phenylalanine / genetics; Polychaeta / enzymology; Polychaeta / genetics; Substrate Specificity / genetics; Tyrosine / genetics; Valine / genetics
TL;DR: The role of tyrosine 38 (Y38), which hydrogen bonds to the hydroxyl group of the substrate, was probed and it was suggested that abolishing this hydrogen bond may open a pathway for the escape of the one-electron product, the phenoxy radical leading to polymeric products. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2006 journal article

Removal of divalent cations induces structural transitions in Red clover necrotic mosaic virus, revealing a potential mechanism for RNA release

JOURNAL OF VIROLOGY, 80(21), 10395–10406.

By: M. Sherman n, R. Guenther n, F. Tama*, T. Sit n, C. Brooks*, A. Mikhailov*, E. Orlova*, T. Baker n, S. Lommel n

Contributors: M. Sherman n, R. Guenther n, F. Tama*, T. Sit n, C. Brooks*, A. Mikhailov*, E. Orlova*, T. Baker n, S. Lommel n

MeSH headings : Amino Acid Sequence; Calcium / metabolism; Capsid / metabolism; Capsid / ultrastructure; Capsid Proteins / genetics; Capsid Proteins / metabolism; Capsid Proteins / ultrastructure; Cations, Divalent / metabolism; Cryoelectron Microscopy; Image Processing, Computer-Assisted; Magnesium / metabolism; Models, Molecular; Molecular Sequence Data; Multiprotein Complexes; RNA, Viral / genetics; RNA, Viral / metabolism; Sequence Homology, Amino Acid; Species Specificity; Static Electricity; Tombusviridae / genetics; Tombusviridae / metabolism; Tombusviridae / ultrastructure
TL;DR: Results indicate that divalent cations play a central role in capsid dynamics and suggest a mechanism for the release of viral RNA in low-divalent-cation environments such as those found within the cytoplasm of a cell. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2006 journal article

The genomic RNA packaging scheme of Red clover necrotic mosaic virus

VIROLOGY, 345(2), 532–539.

By: V. Basnayake n, T. Sit n & S. Lommel n

Contributors: V. Basnayake n, T. Sit n & S. Lommel n

author keywords: RCNMV; virions; UV crosslinking; heating; co-packaging; genome; multipartite; heterodimer; homodimer
MeSH headings : Dimerization; Genome, Viral; Plant Diseases / virology; Plant Viruses / genetics; Plant Viruses / metabolism; RNA, Viral / metabolism; Tobacco / virology; Tombusviridae / genetics; Tombusviridae / metabolism; Trifolium / virology; Virion / metabolism; Virus Assembly
TL;DR: Results indicate that RCNMV virions consist of two distinct populations: (i) virions containing both genomic RNAs; and (ii) virion with multiple copies of RNA-2. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2005 journal article

Cell wall localization of Red clover necrotic mosaic virus movement protein is required for cell-to-cell movement

VIROLOGY, 333(1), 10–21.

By: D. Tremblay n, A. Vaewhongs n, K. Turner n, T. Sit n & S. Lommel n

Contributors: D. Tremblay n, A. Vaewhongs n, K. Turner n, T. Sit n & S. Lommel n

author keywords: RCNMV movement protein; plasmodesmata; GFP; intracellular localization; viral movement; confocal microscopy
MeSH headings : Amino Acid Sequence; Cell Wall / physiology; Cell Wall / virology; Mosaic Viruses / genetics; Mosaic Viruses / physiology; Mutation; Plant Diseases / virology; Plant Viral Movement Proteins; Plants, Genetically Modified; Protein Transport; RNA, Viral; Recombinant Fusion Proteins / metabolism; Tobacco / ultrastructure; Tobacco / virology; Viral Proteins / metabolism; Viral Proteins / physiology
TL;DR: The data suggest the presence of distinct domains in the MP for virus movement, complementarity, and intracellular localization and support a model of MP interacting cooperatively with itself to bind viral RNA, localize to and modify plasmodesmata and effect virus movement. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2005 journal article

Enzyme function of the globin dehaloperoxidase from Amphitrite ornata is activated by substrate binding

BIOCHEMISTRY, 44(48), 15637–15644.

By: J. Belyea n, L. Gilvey n, M. Davis n, M. Godek n, T. Sit n, S. Lommel n, S. Franzen n

Contributors: J. Belyea n, L. Gilvey n, M. Davis n, M. Godek n, T. Sit n, S. Lommel n, S. Franzen n

MeSH headings : Animals; Cloning, Molecular; Enzyme Activation; Escherichia coli / enzymology; Escherichia coli / genetics; Globins / metabolism; Hemoglobins; Hydrogen Peroxide / metabolism; Peroxidases / antagonists & inhibitors; Peroxidases / genetics; Peroxidases / metabolism; Phenols / metabolism; Polychaeta / enzymology; Protein Binding; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism
TL;DR: The present study shows that, unlike other known peroxidases, DHP activity requires the addition of substrate, TBP, prior to the cosubstrate, peroxide. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2004 journal article

Red clover necrotic mosaic virus replication proteins accumulate at the endoplasmic reticulum

VIROLOGY, 320(2), 276–290.

By: K. Turner n, T. Sit n, A. Callaway n, N. Allen n & S. Lommel n

Contributors: K. Turner n, T. Sit n, A. Callaway n, N. Allen n & S. Lommel n

author keywords: RCNMV; polymerase; green fluorescent protein; endoplasmic reticulum; co-localization; Tombusviridae; confocal microscopy
MeSH headings : Endoplasmic Reticulum / metabolism; Endoplasmic Reticulum / virology; Green Fluorescent Proteins; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Microfilament Proteins / genetics; Microfilament Proteins / metabolism; Microscopy, Confocal; Muscle Proteins; Plants, Genetically Modified; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Tobacco / virology; Tombusviridae / genetics; Tombusviridae / metabolism; Tombusviridae / pathogenicity; Trifolium / virology; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Replication
TL;DR: Independent accumulation in, and perturbation of, the ER suggests that p27 and p88 function together in the replication complex, the first report of a member of the Tombusviridae replicating in association with the ER. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2004 journal article

Structural characterization of an intermolecular RNA-RNA interaction involved in the transcription regulation element of a bipartite plant virus

NUCLEIC ACIDS RESEARCH, 32(9), 2819–2828.

By: R. Guenther*, T. Sit, H. Gracz, M. Dolan, H. Townsend, G. Liu, W. Newman, P. Agris, S. Lommel

Contributors: R. Guenther*, T. Sit, H. Gracz, M. Dolan, H. Townsend, G. Liu, W. Newman, P. Agris, S. Lommel

MeSH headings : Base Pairing; Base Sequence; Computer Simulation; Gene Expression Regulation, Viral; Macromolecular Substances; Models, Molecular; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Nucleic Acid Conformation; Promoter Regions, Genetic; RNA, Viral / chemistry; RNA, Viral / metabolism; Regulatory Sequences, Ribonucleic Acid; Tombusviridae / genetics; Transcription, Genetic
TL;DR: The RCNMV TA structural model is similar to those for the Simian retrovirus frameshifting element and the Human immunodeficiency virus-1 dimerization kissing hairpins, suggesting a conservation of form and function. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2001 journal article

A single amino acid mutation in the Carnation ringspot virus capsid protein allows virion formation but prevents systemic infection

JOURNAL OF VIROLOGY, 75(19), 9538–9542.

By: T. Sit n, P. Haikal n, A. Callaway n & S. Lommel n

Contributors: T. Sit n, P. Haikal n, A. Callaway n & S. Lommel n

MeSH headings : Amino Acid Sequence; Amino Acid Substitution; Capsid / genetics; Molecular Sequence Data; Plant Viruses / physiology; Plants / virology; Point Mutation; Virion / physiology; Virulence / genetics; Virus Assembly / genetics
TL;DR: Results provide additional support for the theory that virion formation is necessary but not sufficient for systemic movement with the dianthoviruses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2001 journal article

Spontaneous deletion enhances movement of a cucumber necrosis virus based chimera expressing the red clover necrotic mosaic virus movement protein gene +

Molecular Plant Pathology, 2(1), 13–25.

By: R. Reade*, K. Delroux*, K. Macdonald*, T. Sit n, S. Lommel n & D. Rochon*

Contributors: R. Reade*, K. Delroux*, K. Macdonald*, T. Sit n, S. Lommel n & D. Rochon*

TL;DR: The ability of the CNV replicase to delete unnecessary or deleterious RCNMV sequences and to append the required CNV MP sequences reinforces the role of RNA recombination in the adaptation and evolution of viral genomes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: ORCID
Added: July 21, 2020

2001 review

The multifunctional capsid proteins of plant RNA viruses

[Review of ]. ANNUAL REVIEW OF PHYTOPATHOLOGY, 39(1), 419-+.

By: A. Callaway n, D. Giesman-Cookmeyer n, E. Gillock n, T. Sit n & S. Lommel n

Contributors: A. Callaway n, D. Giesman-Cookmeyer n, E. Gillock n, T. Sit n & S. Lommel n

author keywords: evolution; movement; transmission; resistance; host-factors; biotechnology
MeSH headings : Biotechnology; Capsid / genetics; Capsid / physiology; Genome, Viral; Phylogeny; Plant Diseases / virology; Plant Viruses / genetics; Plant Viruses / physiology; RNA Viruses / genetics; RNA Viruses / physiology; RNA, Viral / genetics; RNA, Viral / physiology
TL;DR: This article summarizes studies of viral coat (capsid) proteins (CPs) of RNA plant viruses and considers the evolution of viral CPs and their multitude of interactions with factors encoded by the virus, host plant, or viral vector that influence the infection and epidemiological facets of plant disease. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2001 article

Tombusviridae

Giesman-Cookmeyer, D., Sit, T. L., & Lommel, S. A. (2001, August). Encyclopedia of Life Sciences.

By: D. Giesman-Cookmeyer n, T. Sit n & S. Lommel n

Source: ORCID
Added: July 21, 2020

1998 journal article

RNA-mediated trans-activation of transcription from a viral RNA

SCIENCE, 281(5378), 829–832.

By: T. Sit n, A. Vaewhongs n & S. Lommel n

Contributors: T. Sit n, A. Vaewhongs n & S. Lommel n

MeSH headings : Base Composition; Base Sequence; DNA, Complementary; Gene Expression; Genes, Reporter; Green Fluorescent Proteins; Luminescent Proteins / genetics; Models, Genetic; Molecular Sequence Data; Mosaic Viruses / genetics; Mutation; Nucleic Acid Conformation; Promoter Regions, Genetic; RNA, Double-Stranded / genetics; RNA, Double-Stranded / metabolism; RNA, Messenger / biosynthesis; RNA, Messenger / genetics; RNA, Viral / biosynthesis; RNA, Viral / chemistry; RNA, Viral / genetics; Sequence Alignment; Transcriptional Activation
TL;DR: A model is proposed in which direct binding of RNA-2 to RNA-1 trans-activates sgRNA synthesis, which is unusual among RNA viruses, which typically rely on protein regulators. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

1998 patent

Trans-activation of transcription from viral RNA

Washington, DC: U.S. Patent and Trademark Office.

By: S. Lommel & T. Sit

Source: NC State University Libraries
Added: August 6, 2018

1995 journal article

Mutational analysis of the cucumber necrosis virus coat protein gene

Virology, 206(1), 38–48.

By: T. Sit*, J. Johnston*, M. Borg*, E. Frison, M. McLean* & D. Rochon*

Contributors: T. Sit*, J. Johnston*, M. Ter Borg*, E. Frison, M. McLean* & D. Rochon*

MeSH headings : Base Sequence; Capsid / genetics; Capsid / physiology; DNA, Viral; Molecular Sequence Data; Plants / virology; Protoplasts; RNA, Viral / metabolism; Sequence Deletion; Tombusvirus / genetics; Tombusvirus / physiology; Virion
TL;DR: Protoplast studies show that PD(-) RNA inoculum does not undergo further deletion in infected protoplasts, suggesting thatPD(-) CP deletion derivatives preferentially accumulate in plants because they have a greater capacity for cell-to-cell movement. (via Semantic Scholar)
Source: ORCID
Added: July 21, 2020

1994 journal article

Coat protein of cucumber necrosis virus is not required for efficient generation or accumulation of defective interfering RNAs

Journal of General Virology, 75(9), 2505–2508.

By: D. Rochon*, R. Finnen* & T. Sit*

Contributors: M. D'a., R. Finnen* & T. Sit*

TL;DR: Accumulation of CNV DI RNAs occurs at equal efficiency in coinoculated plants using either synthetic wild-type CNV genomic RNA as helper or a mutant ofCNV which lacks the coat protein-coding sequence, which shows that the CNV coat protein is not required for efficient accumulation of C NV DI RNA in plants. (via Semantic Scholar)
Source: ORCID
Added: July 21, 2020

1994 journal article

The Minimal 5′ Sequence for in Vitro Initiation of Papaya Mosaic Potexvirus Assembly

Virology, 199(1), 238–242.

By: T. Sit*, D. Leclerc* & M. Abou-Haidar*

Contributors: T. Sit*, D. Leclerc* & M. Abou Haidar*

MeSH headings : Base Sequence; DNA, Viral; Molecular Sequence Data; Mosaic Viruses / genetics; Mosaic Viruses / physiology; Mosaic Viruses / ultrastructure; RNA, Viral; Restriction Mapping; Transcription, Genetic
TL;DR: The minimal 5' initiation of assembly sequence for papaya mosaic virus has been determined by the generation of successive 3' deletion clones from a full-length cDNA copy of the PMV genome followed by in vitro assembly of RNA transcripts. (via Semantic Scholar)
UN Sustainable Development Goal Categories
1. No Poverty (OpenAlex)
Source: ORCID
Added: July 21, 2020

1993 journal article

Cloning and sequence determination of the valS gene, encoding valyl-tRNA synthetase in Lactobacillus casei.

Journal of Bacteriology, 175(8), 2475–2478.

By: B. Taylor*, J. Toy*, T. Sit* & A. Bognar*

Contributors: B. Taylor*, J. Toy*, T. Sit* & A. Bognar*

MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; Genes, Bacterial; Genetic Complementation Test; Lacticaseibacillus casei / enzymology; Lacticaseibacillus casei / genetics; Molecular Sequence Data; Plasmids; Valine-tRNA Ligase / chemistry; Valine-tRNA Ligase / genetics
TL;DR: The DNA sequence of the valS gene from Lactobacillus casei and the predicted amino acid sequence of its valyl-tRNA synthetase product have been determined and an open reading frame coding for a protein of 901 amino acids was found. (via Semantic Scholar)
Source: ORCID
Added: July 21, 2020

1993 journal article

Infectious RNA transcripts derived from cloned cDNA of papaya mosaic virus: effect of mutations to the capsid and polymerase proteins

Journal of General Virology, 74(6), 1133–1140.

By: T. Sit* & M. AbouHaidar*

Contributors: T. Sit* & M. AbouHaidar*

MeSH headings : Base Sequence; Capsid / genetics; Cloning, Molecular; DNA, Viral / genetics; Fruit / microbiology; Molecular Sequence Data; Mosaic Viruses / genetics; Mosaic Viruses / pathogenicity; Mutation; RNA, Messenger / genetics; RNA, Viral / genetics; RNA-Directed DNA Polymerase / genetics; Transcription, Genetic; Virulence
TL;DR: Mutations to the CP suggest that it is required not only for viral assembly but also for some other unidentified function(s) during the replication cycle, suggesting that it exists as a naked RNA species within the host. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: ORCID
Added: July 21, 2020

1990 journal article

Complete nucleotide sequence of clover yellow mosaic virus RNA

Journal of General Virology, 71(9), 1913–1920.

By: T. Sit*, K. White*, S. Holy*, U. Padmanabhan*, M. Eweida*, M. Hiebert*, G. Mackie*, M. AbouHaidar*

Contributors: T. Sit*, K. White*, S. Holy*, U. Padmanabhan*, M. Eweida*, M. Hiebert*, G. Mackie*, M. AbouHaidar*

MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Viral / genetics; Genes, Viral; Molecular Sequence Data; Mosaic Viruses / genetics; Oligonucleotide Probes; RNA, Viral / genetics; Sequence Homology, Nucleic Acid; Viral Proteins / genetics
TL;DR: The entire genomic RNA of clover yellow mosaic virus was sequenced from cDNA clones and run-off cDNA transcripts and shows strong homology with proteins of other potexviruses, especially papaya mosaic virus. (via Semantic Scholar)
Source: ORCID
Added: July 21, 2020

1989 journal article

Highly sensitive and specific non-radioactive biotinylated probes for dot-blot, Southern and colony hybridizations

Journal of Virological Methods, 26(1), 35–43.

By: M. Eweida*, T. Sit*, S. Sira* & M. AbouHaidar*

Contributors: M. Eweida*, T. Sit*, S. Sira* & M. AbouHaidar*

MeSH headings : Adenoviridae / genetics; Adenoviridae / isolation & purification; Biotin; Blotting, Southern; Cloning, Molecular / methods; DNA / genetics; DNA Probes / isolation & purification; DNA, Recombinant / analysis; DNA, Viral / analysis; DNA, Viral / genetics; Nucleic Acid Hybridization; Plant Viruses / genetics; Plant Viruses / isolation & purification
TL;DR: This method has been improved and optimized to produce biotinylated DNA probes for the detection of DNA by dot-blot, Southern and colony hybridization techniques and is highly specific for target DNA even in crude bacterial lysates. (via Semantic Scholar)
Source: ORCID
Added: July 21, 2020

1989 journal article

Molecular cloning of the genome of the carlavirus potato virus S: biotinylated RNA transcripts for virus detection in crude potato extracts

Annals of Applied Biology, 115(2), 253–261.

By: M. Eweida*, T. Sit* & M. Abouhaidar*

Contributors: M. Eweida*, T. Sit* & M. Abouhaidar*

TL;DR: A cDNA library of potato virus S (PVS) in plasmid pBS+ was produced and biotinylated DNA or RNA probes were highly specific with virtually no background coloration in dot-blot hybridisation. (via Semantic Scholar)
Source: ORCID
Added: July 21, 2020

1989 journal article

Nucleotide Sequence of Papaya Mosaic Virus RNA

Journal of General Virology, 70(9), 2325–2331.

By: T. Sit*, M. Abouhaidar* & S. Holy*

Contributors: T. Sit*, M. Abouhaidar* & S. Holy*

MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; Codon / genetics; Genes, Viral; Molecular Sequence Data; Mosaic Viruses / genetics; RNA, Viral / genetics; Sequence Homology, Nucleic Acid; Viral Proteins / genetics
TL;DR: The RNA genome of papaya mosaic virus is 6656 nucleotides long [excluding the poly(A) tail] with six open reading frames (ORFs) more than 200 nucleotide long and the translation products of ORFs 1 to 3 show strong similarity with those of other potexviruses but the ORF 4 protein has only limited similarity with the other Potexvirus OrF 4 proteins of 7K to 11K. (via Semantic Scholar)
Source: ORCID
Added: July 21, 2020

Employment

Updated: March 11th, 2020 11:27

1995 - present

North Carolina State University Raleigh, NC, US
Principal Research Scholar Entomology and Plant Pathology

1993 - 1995

Agriculture and Agri-Food Canada Vancouver, BC, CA
Visiting Scientist

Education

Updated: March 11th, 2020 11:27

1988 - 1993

University of Toronto Toronto, ON, CA
Doctor of Philosophy Microbiology

1985 - 1988

University of Toronto Toronto, ON, CA
Master of Science Microbiology

1981 - 1985

University of Toronto Toronto, ON, CA
Bachelor of Science Microbiology

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