Thomas Makris

Temperature-dependent conformational dynamics govern regioselectivity in a CYP152 decarboxylase

Avila, M. C., Rade, L. L., Souza, A. S., Melo, R. R., Oliveira, C. C., Moraes, M. R., … Zanphorlin, L. M. (2026, February 1). Journal of Biological Chemistry, Vol. 2.

Provision of a Redox Donor to Oxy-Ferrous PrnB Is Required for Pyrrolnitrin Synthesis

McMillan, R. B., Gering, H. E., Ledinina, A. E., Xue, S., Karandikar, S., Lin, S., … Makris, T. M. (2025, August 19). Biochemistry, Vol. 8.

Regulation of ferryl reactivity by the cytochrome P450 decarboxylase OleT

Gering, H. E., Manley, O. M., Holwerda, A. J., Grant, J. L., Ratigan, S. C., & Makris, T. M. (2025, April 6). Journal of Inorganic Biochemistry, Vol. 270.

The Heme Oxygenase-Like Diiron Enzyme HrmI Reveals Altered Regulatory Mechanisms for Dioxygen Activation and Substrate N-Oxygenation

Skirboll, S. S., Gangopadhyay, M., Phan, H. N., Hartsell, J., Mudireddy, A., Hilovsky, D., … Makris, T. M. (2025, August 7). Journal of the American Chemical Society, Vol. 8.

Assembly of a Heterobimetallic Fe/Mn Cofactor in the para-Aminobenzoate Synthase Chlamydia Protein Associating with Death Domains (CADD) Initiates Long-Range Radical Hole-Hopping

Phan, H. N., Swartz, P. D., Gangopadhyay, M., Guo, Y., Smirnov, A. I., & Makris, T. M. (2024, October 29). Biochemistry, Vol. 10.

A Ferric-Superoxide Intermediate Initiates P450-Catalyzed Cyclic Dipeptide Dimerization

Gering, H. E., Li, X., Tang, H., Swartz, P. D., Chang, W.-C., & Makris, T. M. (2023, August 23). Journal of the American Chemical Society, Vol. 8.

Dimer-assisted mechanism of (un)saturated fatty acid decarboxylation for alkene production

Rade, L. L., Generoso, W. C., Das, S., Souza, A. S., Silveira, R. L., Avila, M. C., … Zanphorlin, L. M. (2023, May 22). Proceedings of the National Academy of Sciences, Vol. 120.

Enhancing ferryl accumulation in H2O2-dependent cytochrome P450s

Amaya, J. A., Manley, O. M., Bian, J. C., Rutland, C. D., Leschinsky, N., Ratigan, S. C., & Makris, T. M. (2023, December 20). Journal of Inorganic Biochemistry, Vol. 252.

Excision of a Protein-Derived Amine for p-Aminobenzoate Assembly by the Self-Sacrificial Heterobimetallic Protein CADD

Phan, H. N., Manley, O. M., Skirboll, S. S., Cha, L., Hilovsky, D., Chang, W.-chen, … Makris, T. M. (2023, November 7). Biochemistry, Vol. 62, pp. 3276–3282.

Room-Temperature Aerobic C–CN Bond Activation in Nickel(II) Cyanomethyl Dicarboranyl Complex

Islam, M. J., Park, K. C., Manley, O. M., Smith, M. D., Makris, T. M., & Peryshkov, D. V. (2023, July 12). Organometallics, Vol. 7.

Experimental and Theoretical Examination of the Kinetic Isotope Effect in Cytochrome P450 Decarboxylase OleT

Dutra, M., Amaya, J. A., McElhenney, S., Manley, O. M., Makris, T. M., Rassolov, V., & Garashchuk, S. (2022, May 4). The Journal of Physical Chemistry B, Vol. 126, pp. 3493–3504.

Modeling the Ligand Effect on the Structure of CYP 450 Within the Density Functional Theory

Dutra, M., McElhenney, S., Manley, O., Makris, T., Rassolov, V., & Garashchuk, S. (2022, May 2). The Journal of Physical Chemistry A, Vol. 126, pp. 2818–2824.

Self-sacrificial tyrosine cleavage by an Fe:Mn oxygenase for the biosynthesis of para -aminobenzoate in Chlamydia trachomatis

Manley, O. M., Phan, H. N., Stewart, A. K., Mosley, D. A., Xue, S., Cha, L., … Makris, T. M. (2022, September 19). Proceedings of the National Academy of Sciences, Vol. 119.

Stimuli-Modulated Metal Oxidation States in Photochromic MOFs

Martin, C. R., Park, K. C., Leith, G. A., Yu, J., Mathur, A., Wilson, G. R., … Shustova, N. B. (2022, February 9). Journal of the American Chemical Society, Vol. 144, pp. 4457–4468.

Structural and functional characterization of β-cyanoalanine synthase from Tetranychus urticae

Daneshian, L., Renggli, I., Hanaway, R., Offermann, L. R., Schlachter, C. R., Arriaza, R. H., … Chruszcz, M. (2022, January 19). Insect Biochemistry and Molecular Biology, Vol. 142.

BesC Initiates C–C Cleavage through a Substrate-Triggered and Reactive Diferric-Peroxo Intermediate

Manley, O. M., Tang, H., Xue, S., Guo, Y., Chang, W.-chen, & Makris, T. M. (2021, December 13). Journal of the American Chemical Society, Vol. 12.

Direct Identification of Mixed-Metal Centers in Metal–Organic Frameworks: Cu₃(BTC)₂ Transmetalated with Rh²⁺ Ions

Metavarayuth, K., Ejegbavwo, O., McCarver, G., Myrick, M. L., Makris, T. M., Vogiatzis, K. D., … Chen, D. A. (2020, September 7). The Journal of Physical Chemistry Letters, Vol. 11, pp. 8138–8144.

Enhanced P450 fatty acid decarboxylase catalysis by glucose oxidase coupling and co-assembly for biofuel generation

Zhang, L., Manley, O. M., Ma, D., Yin, Y., Makris, T. M., & Wang, Q. (2020, May 15). Bioresource Technology, Vol. 311.

Microfluidic-assisted polymer-protein assembly to fabricate homogeneous functionalnanoparticles

Zhang, L., Beatty, A., Lu, L., Abdalrahman, A., Makris, T. M., Wang, G., & Wang, Q. (2020, February 22). Materials Science and Engineering C, Vol. 111.

Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues

Blahut, M., Wise, C. E., Bruno, M. R., Dong, G., Makris, T. M., Frantom, P. A., … Outten, F. W. (2019, June 27). Journal of Biological Chemistry, Vol. 294, pp. 12444–12458.

Oxidative Decarboxylase UndA Utilizes a Dinuclear Iron Cofactor

Manley, O. M., Fan, R., Guo, Y., & Makris, T. M. (2019, May 14). Journal of the American Chemical Society, Vol. 141, pp. 8684–8688.

Plasmin-Cleavable Nanoparticles for On-Demand Release of Morphogens in Vascularized Osteogenesis

Kader, S., Monavarian, M., Barati, D., Moeinzadeh, S., Makris, T. M., & Jabbari, E. (2019, June 21). Biomacromolecules, Vol. 20, pp. 2973–2988.

The Diiron Monooxygenase CmlA from Chloramphenicol Biosynthesis Allows Reconstitution of β-Hydroxylation during Glycopeptide Antibiotic Biosynthesis

Kaniusaite, M., Goode, R. J. A., Schittenhelm, R. B., Makris, T. M., & Cryle, M. J. (2019, November 27). ACS Chemical Biology, Vol. 14, pp. 2932–2941.

Cytochrome P450 Decarboxylases

Makris, T. M. (2018, October 1). RSC Metallobiology, pp. 127–143.

Dioxygen Activation by the Biofuel-Generating Cytochrome P450 OleT

Wise, C. E., Hsieh, C. H., Poplin, N. L., & Makris, T. M. (2018, August 27). ACS Catalysis, Vol. 8, pp. 9342–9352.

Enhanced Arylamine N-Oxygenase Activity of Polymer–Enzyme Assemblies by Facilitating Electron-Transferring Efficiency

Zhang, L., Xu, Y., Makris, T. M., & Wang, Q. (2018, February 20). Biomacromolecules, Vol. 19, pp. 918–925.

Structural and functional characterization of an intradiol ring-cleavage dioxygenase from the polyphagous spider mite herbivore Tetranychus urticae Koch

Schlachter, C. R., Daneshian, L., Amaya, J., Klapper, V., Wybouw, N., Borowski, T., … Chruszcz, M. (2018, December 5). Insect Biochemistry and Molecular Biology, Vol. 107, pp. 19–30.

Structure and function of the cytochrome P450 peroxygenase enzymes

Munro, A. W., McLean, K. J., Grant, J. L., & Makris, T. M. (2018, February 6). Biochemical Society Transactions, Vol. 46, pp. 183–196.

A Distal Loop Controls Product Release and Chemo- and Regioselectivity in Cytochrome P450 Decarboxylases

Amaya, J. A., Rutland, C. D., Leschinsky, N., & Makris, T. M. (2017, December 11). Biochemistry, Vol. 57, pp. 344–353.

Activation of C–H Bonds of Alkyl- and Arylnitriles by the TaCl₅–PPh₃ Lewis Pair

Rahman, M. M., Smith, M. D., Amaya, J. A., Makris, T. M., & Peryshkov, D. V. (2017, September 13). Inorganic Chemistry, Vol. 56, pp. 11798–11803.

Catalytic N−H Bond Activation and Breaking by a Well‐Defined Co^II₁O₄ Site of a Heterogeneous Catalyst

Khivantsev, K., Biancardi, A., Fathizadeh, M., Almalki, F., Grant, J. L., Tien, H. N., … Yu, M. (2017, October 3). ChemCatChem, Vol. 10, pp. 736–742.

On the Structure and Reaction Mechanism of Human Acireductone Dioxygenase

Miłaczewska, A., Kot, E., Amaya, J. A., Makris, T. M., Zając, M., Korecki, J., … Borowski, T. (2017, November 29). Chemistry - A European Journal, Vol. 24, pp. 5225–5237.

Persistent Radicals of Self‐assembled Benzophenone bis‐Urea Macrocycles: Characterization and Application as a Polarizing Agent for Solid‐state DNP MAS Spectroscopy

DeHaven, B. A., Tokarski, J. T., Korous, A. A., Mentink‐Vigier, F., Makris, T. M., Brugh, A. M., … Shimizu, L. S. (2017, April 19). Chemistry - A European Journal, Vol. 23, pp. 8315–8319.

Recruitment and Regulation of the Non-ribosomal Peptide Synthetase Modifying Cytochrome P450 Involved in Nikkomycin Biosynthesis

Wise, C. E., & Makris, T. M. (2017, March 16). ACS Chemical Biology, Vol. 12, pp. 1316–1326.

The Enigmatic P450 Decarboxylase OleT Is Capable of, but Evolved To Frustrate, Oxygen Rebound Chemistry

Hsieh, C. H., Huang, X., Amaya, J. A., Rutland, C. D., Keys, C. L., Groves, J. T., … Makris, T. M. (2017, June 12). Biochemistry, Vol. 56, pp. 3347–3357.

Catalytic strategy for carbon−carbon bond scission by the cytochrome P450 OleT

Grant, J. L., Mitchell, M. E., & Makris, T. M. (2016, August 23). Proceedings of the National Academy of Sciences, Vol. 113, pp. 10049–10054.

Divergent mechanisms of iron-containing enzymes for hydrocarbon biosynthesis

Wise, C. E., Grant, J. L., Amaya, J. A., Ratigan, S. C., Hsieh, C. H., Manley, O. M., & Makris, T. M. (2016, December 21). JBIC Journal of Biological Inorganic Chemistry, Vol. 22, pp. 221–235.

Expanding the substrate scope and reactivity of cytochrome P450 OleT

Hsieh, C. H., & Makris, T. M. (2016, May 29). Biochemical and Biophysical Research Communications, Vol. 476, pp. 462–466.

Mixed regiospecificity compromises alkene synthesis by a cytochrome P450 peroxygenase from Methylobacterium populi

Amaya, J. A., Rutland, C. D., & Makris, T. M. (2016, February 28). Journal of Inorganic Biochemistry, Vol. 158, pp. 11–16.

An Unusual Peroxo Intermediate of the Arylamine Oxygenase of the Chloramphenicol Biosynthetic Pathway

Makris, T. M., Vu, V. V., Meier, K. K., Komor, A. J., Rivard, B. S., Münck, E., … Lipscomb, J. D. (2015, January 7). Journal of the American Chemical Society, Vol. 137, pp. 1608–1617.

Decarboxylation of Fatty Acids to Terminal Alkenes by Cytochrome P450 Compound I

Grant, J. L., Hsieh, C. H., & Makris, T. M. (2015, April 5). Journal of the American Chemical Society, Vol. 137, pp. 4940–4943.

Dinuclear Iron Cluster‐Containing OxygenaseCmlA

Knoot, C. J., Makris, T. M., & Lipscomb, J. D. (2015, June 14). Encyclopedia of Inorganic and Bioinorganic Chemistry.

Synthesis, characterization, and properties of reduced europium molybdates and tungstates

Abeysinghe, D., Gerke, B., Morrison, G., Hsieh, C. H., Smith, M. D., Pöttgen, R., … Loye, H.-C. (2015, May 18). Journal of Solid State Chemistry, Vol. 229, pp. 173–180.

Catalase (KatA) Plays a Role in Protection against Anaerobic Nitric Oxide in Pseudomonas aeruginosa

Su, S., Panmanee, W., Wilson, J. J., Mahtani, H. K., Li, Q., VanderWielen, B. D., … Hassett, D. J. (2014, March 24). PLoS ONE, Vol. 9.

Cyanobacterial Aldehyde Deformylase Oxygenation of Aldehydes Yields n – 1 Aldehydes and Alcohols in Addition to Alkanes

Aukema, K. G., Makris, T. M., Stoian, S. A., Richman, J. E., Münck, E., Lipscomb, J. D., & Wackett, L. P. (2013, August 16). ACS Catalysis, Vol. 3, pp. 2228–2238.

Structure of a Dinuclear Iron Cluster-Containing β-Hydroxylase Active in Antibiotic Biosynthesis

Makris, T. M., Knoot, C. J., Wilmot, C. M., & Lipscomb, J. D. (2013, August 27). Biochemistry, Vol. 52, pp. 6662–6671.

Structural and Molecular Characterization of Iron-sensing Hemerythrin-like Domain within F-box and Leucine-rich Repeat Protein 5 (FBXL5)

Thompson, J. W., Salahudeen, A. A., Chollangi, S., Ruiz, J. C., Brautigam, C. A., Makris, T. M., … Bruick, R. K. (2012, January 18). Journal of Biological Chemistry, Vol. 287, pp. 7357–7365.

Active-Site Structure of a β-Hydroxylase in Antibiotic Biosynthesis

Vu, V. V., Makris, T. M., Lipscomb, J. D., & Que, L. (2011, April 20). Journal of the American Chemical Society, Vol. 133, pp. 6938–6941.

A family of diiron monooxygenases catalyzing amino acid beta-hydroxylation in antibiotic biosynthesis

Makris, T. M., Chakrabarti, M., Münck, E., & Lipscomb, J. D. (2010, August 16). Proceedings of the National Academy of Sciences, Vol. 107, pp. 15391–15396.

Resonance Raman Characterization of the Peroxo and Hydroperoxo Intermediates in Cytochrome P450

Denisov, I. G., Mak, P. J., Makris, T. M., Sligar, S. G., & Kincaid, J. R. (2008, July 16). The Journal of Physical Chemistry A, Vol. 112, pp. 13172–13179.

Activation of Molecular Oxygen by Cytochrome P450

Makris, T. M., Denisov, I., Schlichting, I., & Sligar, S. G. (2007, February 4). Cytochrome P450: Structure, Mechanism, and Biochemistry: Third Edition, pp. 149–182.

Alteration of P450 Distal Pocket Solvent Leads to Impaired Proton Delivery and Changes in Heme Geometry

Makris, T. M., Koenig, K., Schlichting, I., & Sligar, S. G. (2007, November 15). Biochemistry, Vol. 46, pp. 14129–14140.

Resonance Raman Detection of the Hydroperoxo Intermediate in the Cytochrome P450 Enzymatic Cycle

Mak, P. J., Denisov, I. G., Victoria, D., Makris, T. M., Deng, T., Sligar, S. G., & Kincaid, J. R. (2007, April 27). Journal of the American Chemical Society, Vol. 129, pp. 6382–6383.

Cytochrome P450 Compound I

Newcomb, M., Zhang, R., Chandrasena, R. E. P., Halgrimson, J. A., Horner, J. H., Makris, T. M., & Sligar, S. G. (2006, March 17). Journal of the American Chemical Society, Vol. 128, pp. 4580–4581.

The status of high-valent metal oxo complexes in the P450 cytochromes

Makris, T. M., Koenig, K., Schlichting, I., & Sligar, S. G. (2006, March 1). Journal of Inorganic Biochemistry, Vol. 100, pp. 507–518.

A retinoic acid binding cytochrome P450: CYP120A1 from Synechocystis sp. PCC 6803

Ke, N., Baudry, J., Makris, T. M., Schuler, M. A., & Sligar, S. G. (2005, February 3). Archives of Biochemistry and Biophysics, Vol. 436, pp. 110–120.

Structure and Chemistry of Cytochrome P450

Denisov, I. G., Makris, T. M., Sligar, S. G., & Schlichting, I. (2005, April 21). Chemical Reviews, Vol. 105, pp. 2253–2277.

The influence of substrate on the spectral properties of oxyferrous wild-type and T252A cytochrome P450-CAM

Sono, M., Perera, R., Jin, S., Makris, T. M., Sligar, S. G., Bryson, T. A., & Dawson, J. H. (2005, January 13). Archives of Biochemistry and Biophysics, Vol. 436, pp. 40–49.

Thirty years of microbial P450 monooxygenase research: Peroxo-heme intermediates—The central bus station in heme oxygenase catalysis

Sligar, S. G., Makris, T. M., & Denisov, I. G. (2005, August 25). Biochemical and Biophysical Research Communications, Vol. 338, pp. 346–354.

Epoxidation of Olefins by Hydroperoxo−Ferric Cytochrome P450

Jin, S., Makris, T. M., Bryson, T. A., Sligar, S. G., & Dawson, J. H. (2003, March 1). Journal of the American Chemical Society, Vol. 125, pp. 3406–3407.

Haem-oxygen reactive intermediates: catalysis by the two-step

Makris, T. M., Denisov, I. G., & Sligar, S. G. (2003, June 1). Biochemical Society Transactions, Vol. 31, pp. 516–519.

Resonance Raman Spectroscopic Studies of Hydroperoxo-Myoglobin at Cryogenic Temperatures

Ibrahim, M., Denisov, I. G., Makris, T. M., Kincaid, J. R., & Sligar, S. G. (2003, October 18). Journal of the American Chemical Society, Vol. 125, pp. 13714–13718.

Cryoradiolysis for the study of P450 reaction intermediates

Denisov, I. G., Makris, T. M., & Sugar, S. G. (2002, January 1). Methods in Enzymology on CD-ROM/Methods in Enzymology, Vol. 357, pp. 103–115.

Formation and Decay of Hydroperoxo-Ferric Heme Complex in Horseradish Peroxidase Studied by Cryoradiolysis

Denisov, I. G., Makris, T. M., & Sligar, S. G. (2002, November 1). Journal of Biological Chemistry, Vol. 277, pp. 42706–42710.

MECHANISTIC ENZYMOLOGY OF OXYGEN ACTIVATION BY THE CYTOCHROMES P450

Makris, T. M., Davydov, R., Denisov, I. G., Hoffman, B. M., & Sligar, S. G. (2002, January 1). Drug Metabolism Reviews, Vol. 34, pp. 691–708.

Cryotrapped Reaction Intermediates of Cytochrome P450 Studied by Radiolytic Reduction with Phosphorus-32

Denisov, I. G., Makris, T. M., & Sligar, S. G. (2001, April 1). Journal of Biological Chemistry, Vol. 276, pp. 11648–11652.

Hydroxylation of Camphor by Reduced Oxy-Cytochrome P450cam:  Mechanistic Implications of EPR and ENDOR Studies of Catalytic Intermediates in Native and Mutant Enzymes

Davydov, R., Makris, T. M., Kofman, V., Werst, D. E., Sligar, S. G., & Hoffman, B. M. (2001, January 30). Journal of the American Chemical Society, Vol. 123, pp. 1403–1415.

EPR and ENDOR of Catalytic Intermediates in Cryoreduced Native and Mutant Oxy-Cytochromes P450cam:  Mutation-Induced Changes in the Proton Delivery System

Davydov, R., Macdonald, I. D. G., Makris, T. M., Sligar, S. G., & Hoffman, B. M. (1999, October 30). Journal of the American Chemical Society, Vol. 121, pp. 10654–10655.