Timothy S. Collier

Works (9)

Updated: July 18th, 2023 21:14

2016 article

Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts

Sarkar, P., Mischler, A., Randall, S. M., Collier, T. S., Dorman, K. F., Boggess, K. A., … Rao, B. M. (2016, July 5). Journal of Proteome Research, Vol. 15, pp. 2433–2444.

By: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n , B. Rao n 

Contributors: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n , B. Rao n 

author keywords: quantitative mass spectrometry; SILAC; human embryonic stem cell; trophoblast; epigenetic factor
MeSH headings : Cell Differentiation; DNA (Cytosine-5-)-Methyltransferases / genetics; Epigenomics; Female; Gene Expression / genetics; Human Embryonic Stem Cells / cytology; Humans; Placenta / chemistry; Placenta / cytology; Pregnancy; Transcription Factors / genetics; Trophoblasts / chemistry; Trophoblasts / cytology
topics (OpenAlex): Epigenetics and DNA Methylation; Pluripotent Stem Cells Research; Renal and related cancers
TL;DR: The results support the use of hESC-derived trophoblasts as a model for placental troPHoblasts, which will enable further investigation of epigenetic factors involved in human trophoblast development, and hypothesized that nuclear proteomics of h ESPCs may be used for screening epigenetic factor proteins expressed by primary trophblasts in human placental tissue. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2012 article

Analytical strategies for the global quantification of intact proteins

Collier, T. S., & Muddiman, D. C. (2012, July 21). Amino Acids, Vol. 43, pp. 1109–1117.

By: T. Collier n & D. Muddiman n 

author keywords: Proteomics; Intact proteins; Quantitation; Mass spectrometry
MeSH headings : Animals; Humans; Mass Spectrometry; Proteome / chemistry; Proteome / isolation & purification; Proteome / metabolism; Proteomics; Staining and Labeling; Two-Dimensional Difference Gel Electrophoresis
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: Techniques contained in this review include the more traditional and widely employed methodology of differential gel electrophoresis and more recently developed mass spectrometry-based techniques including metabolic labeling, chemical labeling, and label-free methodologies. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Comparison of stable‐isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression

Collier, T. S., Randall, S. M., Sarkar, P., Rao, B. M., Dean, R. A., & Muddiman, D. C. (2011, August 4). Rapid Communications in Mass Spectrometry, Vol. 25, pp. 2524–2532.

By: T. Collier n, S. Randall n, P. Sarkar n, B. Rao n , R. Dean n  & D. Muddiman n 

Contributors: T. Collier n, S. Randall n, P. Sarkar n, B. Rao n , R. Dean n  & D. Muddiman n 

MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Amino Acids / metabolism; Animals; Cell Differentiation; Cell Line; Ectoderm; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proteins / analysis; Proteins / chemistry; Proteins / metabolism; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The comparison of stable-isotope labeling of amino acids in cell culture (SILAC) with spectral counting for the quantification of human embryonic stem cells as they differentiate toward the trophectoderm at three time points is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 article

The subcellular proteome of undifferentiated human embryonic stem cells

Sarkar, P., Collier, T. S., Randall, S. M., Muddiman, D. C., & Rao, B. M. (2011, December 5). PROTEOMICS, Vol. 12, pp. 421–430.

By: P. Sarkar n, T. Collier n, S. Randall n, D. Muddiman n  & B. Rao n 

Contributors: P. Sarkar n, T. Collier n, S. Randall n, D. Muddiman n  & B. Rao n 

author keywords: Cell biology; Extracellular proteins; Human embryonic stem cells; Subcellular fractionation; Subcellular proteomics
MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned; Cytosol / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endoplasmic Reticulum / genetics; Endoplasmic Reticulum / metabolism; Gene Expression Regulation, Developmental; Golgi Apparatus / genetics; Golgi Apparatus / metabolism; Humans; Membrane Proteins / genetics; Membrane Proteins / metabolism; Mice; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Signal Transduction / genetics; Subcellular Fractions / metabolism
topics (OpenAlex): Cellular transport and secretion; CRISPR and Genetic Engineering; Advanced biosensing and bioanalysis techniques
TL;DR: The results show that systematic subcellular proteome analysis is a valuable tool for studying hESC biology and shows that proteomic analysis of the endoplasmic reticulum and Golgi compartments is a powerful alternative approach to identify secreted proteins since these are synthesized in the ER and transit through the Golgi. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 article

Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics

Collier, T. S., Sarkar, P., Franck, W. L., Rao, B. M., Dean, R. A., & Muddiman, D. C. (2010, September 16). Analytical Chemistry, Vol. 82, pp. 8696–8702.

By: T. Collier n, P. Sarkar  n, W. Franck n, B. Rao n , R. Dean n  & D. Muddiman n 

Contributors: T. Collier n, P. Sarkar  n, W. Franck n, B. Rao n , R. Dean n  & D. Muddiman n 

MeSH headings : Amino Acids / analysis; Animals; Cell Line; Humans; Isotope Labeling / methods; Mice; Proteomics / methods
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: A direct comparison of SILAC to label-free quantification by spectral counting of an identical set of data from the bottom-up proteomic analysis of human embryonic stem cells, finding that both strategies result in a similar number of protein identifications. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 article

Quantitative top-down proteomics of SILAC labeled human embryonic stem cells

Collier, T. S., Sarkar, P., Rao, B., & Muddiman, D. C. (2010, February 9). Journal of the American Society for Mass Spectrometry, Vol. 21, pp. 879–889.

By: T. Collier n, P. Sarkar  n, B. Rao n  & D. Muddiman n 

Contributors: T. Collier n, P. Sarkar  n, B. Rao n  & D. Muddiman n 

MeSH headings : Amino Acid Sequence; Animals; Arginine / chemistry; Arginine / metabolism; Carbon Isotopes; Cell Line; Embryonic Stem Cells / chemistry; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Lysine / chemistry; Lysine / metabolism; Mass Spectrometry / methods; Mice; Proteome / analysis; Proteomics / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Amino Acid Enzymes and Metabolism
TL;DR: This study has confidently identified 11 proteins by accurate intact mass, MS/MS, and amino acid counting facilitated by SILAC labeling, which to the best of the authors' knowledge represents the first top-down analysis of hESCs. (via Semantic Scholar)
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Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: August 6, 2018

2009 article

Proteomics Characterization of Cell Membrane Blebs in Human Retinal Pigment Epithelium Cells

Alcazar, O., Hawkridge, A. M., Collier, T. S., Cousins, S. W., Bhattacharya, S. K., Muddiman, D. C., & Marin-Castano, M. E. (2009, June 30). Molecular & Cellular Proteomics, Vol. 8, pp. 2201–2211.

By: O. Alcazar *, A. Hawkridge n, T. Collier n, S. Cousins *, S. Bhattacharya *, D. Muddiman n , M. Marin-Castano*

MeSH headings : Aged; Animals; Antioxidants / pharmacology; Basigin / analysis; Cell Line; Cell Membrane / chemistry; Cell Membrane / drug effects; Cell Membrane / ultrastructure; Cell Surface Extensions / chemistry; Cell Surface Extensions / drug effects; Cell Surface Extensions / ultrastructure; Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Epithelial Cells / chemistry; Epithelial Cells / cytology; Epithelial Cells / drug effects; Extracellular Matrix / chemistry; Extracellular Matrix / metabolism; Eye Proteins / analysis; Humans; Hydroquinones / pharmacology; Macular Degeneration / pathology; Matrix Metalloproteinase 14 / analysis; Matrix Metalloproteinase 2 / analysis; Molecular Sequence Data; Proteomics / methods; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Retinal Pigment Epithelium / chemistry; Retinal Pigment Epithelium / cytology; Smoke; Smoking / adverse effects; Tandem Mass Spectrometry; Tobacco / chemistry
topics (OpenAlex): Retinal Diseases and Treatments; Retinal Development and Disorders; Mosquito-borne diseases and control
TL;DR: Basigin and matrix metalloproteinase-14, key proteins involved in extracellular matrix remodeling, were identified in RPE blebs and shown to be more prevalent in AMD patients. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Microscopic, chemical and molecular methods for examining fossil specimens

Comptes Rendus Palevol, 7(2-3), 159–184.

By: M. Schweitzer n , R. Avci *, T. Collier n & M. Goodwin *

author keywords: molecular preservation; diagenesis; fossil; ancient DNA; ancient protein; dinosaur; mass spectrometry
topics (OpenAlex): Paleontology and Stratigraphy of Fossils; Evolution and Paleontology Studies; Geology and Paleoclimatology Research
TL;DR: This review describes some of these powerful new analytical tools, the data that may be accessible through their use, advantages and limitations, and how they can be applied to fossil material to elucidate characteristics of extinct organisms and their paleoecological environments. (via Semantic Scholar)
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15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Top-Down Identification and Quantification of Stable Isotope Labeled Proteins from Aspergillus flavus Using Online Nano-Flow Reversed-Phase Liquid Chromatography Coupled to a LTQ-FTICR Mass Spectrometer

Collier, T. S., Hawkridge, A. M., Georgianna, D. R., Payne, G. A., & Muddiman, D. C. (2008, May 31). Analytical Chemistry, Vol. 80, pp. 4994–5001.

By: T. Collier n, A. Hawkridge n, D. Georgianna n, G. Payne n & D. Muddiman n 

MeSH headings : Arginine / chemistry; Arginine / metabolism; Aspergillus flavus / chemistry; Aspergillus flavus / metabolism; Carbon Radioisotopes / chemistry; Chromatography, Liquid / methods; Fourier Analysis; Fungal Proteins / analysis; Fungal Proteins / chemistry; Fungal Proteins / metabolism; Isotope Labeling; Tandem Mass Spectrometry / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: The qualitative and quantitative top-down characterization of proteins derived from SILAC-labeled Aspergillus flavus is described using nanoflow reversed-phase liquid chromatography directly coupled to a linear ion trap Fourier transform ion cyclotron resonance mass spectrometer (nLC-LTQ-FTICR-MS). (via Semantic Scholar)
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2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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