@article{le_bassey_nguyen-vo_park_2024, title={Achieving high titer and yield in the bioconversion of <sc>l</sc>-threonine to 2-hydroxybutyric acid with <i>Escherichia coli</i> BL21}, volume={4}, ISSN={["2662-7663"]}, DOI={10.1007/s43393-023-00224-w}, number={2}, journal={SYSTEMS MICROBIOLOGY AND BIOMANUFACTURING}, author={Le, Thai and Bassey, Bassey Friday and Nguyen-Vo, Thuan Phu and Park, Sunghoon}, year={2024}, month={Apr}, pages={708–715} }
 @article{gaur_nguyen-vo_islam_bassey_kim_ainala_shin_park_2024, title={Efficient bioproduction of poly(3-hydroxypropionate) homopolymer using engineered <i>Escherichia coli</i> strains}, volume={397}, ISSN={["1873-2976"]}, DOI={10.1016/j.biortech.2024.130469}, journal={BIORESOURCE TECHNOLOGY}, author={Gaur, Vivek Kumar and Nguyen-Vo, Thuan Phu and Islam, Tayyab and Bassey, Bassey Friday and Kim, Miri and Ainala, Satish Kumar and Shin, Kyusoon and Park, Sunghoon}, year={2024}, month={Apr} }
 @article{islam_nguyen-vo_cho_lee_gaur_park_2023, title={Metabolic engineering of <i>Escherichia coli</i> for enhanced production of 1,3-butanediol from glucose}, volume={389}, ISSN={["1873-2976"]}, DOI={10.1016/j.biortech.2023.129814}, abstractNote={1,3-Butanediol (1,3-BDO) finds versatile applications in the cosmetic, chemical, and food industries. This study focuses on the metabolic engineering of Escherichia coli K12 to achieve efficient production of 1,3-BDO from glucose via acetoacetyl-CoA, 3-hydroxybutyryl-CoA, and 3-hydroxybutyraldehyde. The accumulation of an intermediary metabolite (pyruvate) and a byproduct (3-hydroxybutyric acid) was reduced by disruption of the negative transcription factor (PdhR) for pyruvate dehydrogenase complex (PDHc) and employing an efficient alcohol dehydrogenase (YjgB), respectively. Additionally, to improve NADPH availability, carbon flux was redirected from the Embden-Meyerhof-Parnas (EMP) pathway to the Entner-Doudoroff (ED) pathway. One resulting strain achieved a record-high titer of 790 mM (∼71.1 g/L) with a yield of 0.65 mol/mol for optically pure (R)-1,3-BDO, with an enantiomeric excess (e.e.) value of 98.5 %. These findings are useful in the commercial production of 1,3-BDO and provide valuable insights into the development of an efficient cell factory for other acetyl-CoA derivatives.}, journal={BIORESOURCE TECHNOLOGY}, author={Islam, Tayyab and Nguyen-Vo, Thuan Phu and Cho, Seunghyun and Lee, Junhak and Gaur, Vivek Kumar and Park, Sunghoon}, year={2023}, month={Dec} }
 @article{islam_nguyen-vo_gaur_lee_park_2023, title={Metabolic engineering of Escherichia coli for biological production of 1, 3-Butanediol}, volume={376}, ISSN={["1873-2976"]}, DOI={10.1016/j.biortech.2023.128911}, abstractNote={The production of 1,3-butanediol (1,3-BDO) from glucose was investigated using Escherichia coli as the host organism. A pathway was engineered by overexpressing genes phaA (acetyl-CoA acetyltransferase), phaB (acetoacetyl-CoA reductase), bld (CoA-acylating aldehyde dehydrogenase), and yqhD (alcohol dehydrogenase). The expression levels of these genes were optimized to improve 1,3-BDO production and pathways that compete with 1,3-BDO synthesis were disrupted. Culture conditions were also optimized, including the C: N ratio, aeration, induction time, temperature, and supplementation of amino acids, resulting in a strain that could produce 1,3-BDO at 257 mM in 36 h, with a yield of 0.51 mol/mol in a fed-batch bioreactor experiment. To the best of our knowledge, this is the highest titer of 1,3-BDO production ever reported using biological methods, and our findings provide a promising strategy for the development of microbial cell factories for the sustainable synthesis of other acetyl-CoA-derived chemicals.}, journal={BIORESOURCE TECHNOLOGY}, author={Islam, Tayyab and Nguyen-Vo, Thuan Phu and Gaur, Vivek Kumar and Lee, Junhak and Park, Sunghoon}, year={2023}, month={May} }