@article{kim_jahan_deltchev_lavrik_reukov_minko_2023, title={Strategy for Nonenzymatic Harvesting of Cells via Decoupling of Adhesive and Disjoining Domains of Nanostructured Stimulus-Responsive Polymer Films}, ISSN={["1944-8252"]}, DOI={10.1021/acsami.3c11296}, abstractNote={The nanostructured polymer film introduces a novel mechanism of nonenzymatic cell harvesting by decoupling solid cell-adhesive and soft stimulus-responsive cell-disjoining areas on the surface. The key characteristics of this architecture are the decoupling of adhesion from detachment and the impermeability to the integrin protein complex of the adhesive domains. This surface design eliminates inherent limitations of thermoresponsive coatings, namely, the necessity for the precise thickness of the coating, grafting or cross-linking density, and material of the basal substrate. The concept is demonstrated with nanostructured thermoresponsive films made of cell-adhesive epoxy photoresist domains and cell-disjoining poly(N-isopropylacrylamide) brush domains.}, journal={ACS APPLIED MATERIALS & INTERFACES}, author={Kim, Yongwook and Jahan, Ummay Mowshome and Deltchev, Alexander Pennef and Lavrik, Nickolay and Reukov, Vladimir and Minko, Sergiy}, year={2023}, month={Oct} }
 @article{mahmood_suh_ali_sefat_jahan_huang_gilger_gluck_2022, title={Induced Pluripotent Stem Cell-Derived Corneal Cells: Current Status and Application}, volume={8}, ISSN={["2629-3277"]}, url={https://doi.org/10.1007/s12015-022-10435-8}, DOI={10.1007/s12015-022-10435-8}, abstractNote={Deficiency and dysfunction of corneal cells leads to the blindness observed in corneal diseases such as limbal stem cell deficiency (LSCD) and bullous keratopathy. Regenerative cell therapies and engineered corneal tissue are promising treatments for these diseases [1]. However, these treatments are not yet clinically feasible due to inadequate cell sources. The discovery of induced pluripotent stem cells (iPSCs) by Shinya Yamanaka has provided a multitude of opportunities in research because iPSCs can be generated from somatic cells, thus providing an autologous and unlimited source for corneal cells. Compared to other stem cell sources such as mesenchymal and embryonic, iPSCs have advantages in differentiation potential and ethical concerns, respectively. Efforts have been made to use iPSCs to model corneal disorders and diseases, drug testing [2], and regenerative medicine [1]. Autologous treatments based on iPSCs can be exorbitantly expensive and time-consuming, but development of stem cell banks with human leukocyte antigen (HLA)- homozygous cell lines can provide cost- and time-efficient allogeneic alternatives. In this review, we discuss the early development of the cornea because protocols differentiating iPSCs toward corneal lineages rely heavily upon recapitulating this development. Differentiation of iPSCs toward corneal cell phenotypes have been analyzed with an emphasis on feeder-free, xeno-free, and well-defined protocols, which have clinical relevance. The application, challenges, and potential of iPSCs in corneal research are also discussed with a focus on hurdles that prevent clinical translation.}, journal={STEM CELL REVIEWS AND REPORTS}, author={Mahmood, Nasif and Suh, Taylor Cook and Ali, Kiran M. and Sefat, Eelya and Jahan, Ummay Mowshome and Huang, Yihan and Gilger, Brian C. and Gluck, Jessica M.}, year={2022}, month={Aug} }