Works (19)

Updated: July 5th, 2023 16:02

2011 journal article

Effects of temperature during soybean seed development on defense-related gene expression and fungal pathogen accumulation

BIOTECHNOLOGY LETTERS, 33(12), 2397–2404.

By: R. Upchurch n & M. Ramirez n

author keywords: Defense-related gene expression in seeds; Fungal pathogens; Glycine max; Temperature regimen
MeSH headings : Ascomycota / physiology; Gene Expression Regulation, Plant / physiology; Plant Proteins / metabolism; Seeds / microbiology; Seeds / physiology; Soybeans / microbiology; Soybeans / physiology; Temperature
TL;DR: Temperature regimens applied during seed development produced significant changes in seed defense-related gene expression both pre- and post inoculation and the hypothesis that global climate change may alter plant–pathogen interactions and thereby potentially crop productivity is supported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Soybean Plastidal Omega-3 Fatty Acid Desaturase Genes GmFAD7 and GmFAD8: Structure and Expression

CROP SCIENCE, 51(4), 1673–1682.

By: R. Upchurch n & M. Ramirez n

TL;DR: The genomic structure and deduced amino acid sequence of soybean FAD7 and FAD8 are similar to other higher plant plastidal w-3 desaturases: eight exons and seven introns, predicted proteins of 453 amino acid residues containing three conserved histidine motifs, amino terminal chloroplast transit peptides, and molecular masses of 51.3 and 51.4 kDa. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Gene Expression Profiles of Soybeans with Mid-Oleic Acid Seed Phenotype

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 87(8), 857–864.

By: R. Upchurch n & M. Ramirez n

author keywords: GmFATB1a; GmSACPD-A, -B, -C; GmFAD2-1A,-1B; GmFAD2-2; GmFAD2-3; Allele; Oleic acid; qRT-PCR
TL;DR: This finding suggests that of the several soybean genomic loci known or suspected to be involved in oleic acid phenotype, some are likely to encode genes involved in regulation of transcription of the oleate biosynthetic genes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Mutations in a Delta(9)-Stearoyl-ACP-Desaturase Gene Are Associated with Enhanced Stearic Acid Levels in Soybean Seeds

CROP SCIENCE, 48(6), 2305–2313.

By: P. Zhang n, J. Burton n, R. Upchurch n, E. Whittle*, J. Shanklin* & R. Dewey n

TL;DR: A newly identifi ed soybean gene is reported, designated SACPD-C, that encodes a unique isoform of Δ 9 –stearoyl-ACP-desaturase, the enzyme responsible for converting stearic acid to oleic acid (18:1), suggesting that the encoded desaturase functions to enhance oleIC acid biosynthetic capacity as the immature seed is actively engaged in triacylglycerol production and storage. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Relationships between oleic and linoleic acid content and seed colonization by Cercospora kikuchii and Diaporthe phaseolorum

PLANT DISEASE, 92(7), 1038–1042.

By: H. Xue n, R. Upchurch* & P. Kwanyuen*

TL;DR: The data suggest that the O/L ratio may be related to soybean seed colonization by C. kikuchii, but there is no evidence of a relationship with D. phaseolorum var. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Effect of temperature on delta-9 stearoyl-ACP and microsomal omega-6 desaturase gene expression and fatty acid content in developing soybean seeds

CROP SCIENCE, 47(4), 1698–1704.

By: G. Byfield n & R. Upchurch n

TL;DR: It is concluded that environmental temperature modulates oleic and linoleic acid in developing seed through regulated FAD2-1 gene expression, but temperature modulation of stearic acid content in wildtype soybean may be more complex, involving in addition to SAD-A and -B, plastid thioesterase genes FATA and FATB. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Ergosterol as a quantifiable biomass marker for Diaporthe phaseolorum and Cercospora kikuchii

PLANT DISEASE, 90(11), 1395–1398.

By: H. Xue n, R. Upchurch n & P. Kwanyuen n

TL;DR: Results show that ergosterol content can be used to quantify colonization of soybean seed by both pathogens. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Two genes from soybean encoding soluble Delta 9 stearoyl-ACP desaturases

CROP SCIENCE, 46(2), 840–846.

By: G. Byfield n, H. Xue n & R. Upchurch n

TL;DR: Biochemical analysis of the proteins encoded by these two SACPD genes may reveal whether the amino acid variability uncovered in this study has any relation to enzyme activity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Expression of the cercosporin transporter, CFP, in tobacco reduces frog-eye lesion size

BIOTECHNOLOGY LETTERS, 27(20), 1543–1550.

By: R. Upchurch n, M. Rose n, M. Eweida n & W. Zuo n

author keywords: cercosporin; frog-eye leaf spot; major facilitator; plant genetic engineering; toxin efflux
MeSH headings : Ascomycota / genetics; Fungal Proteins / biosynthesis; Fungal Proteins / genetics; Membrane Transport Proteins / biosynthesis; Membrane Transport Proteins / genetics; Plant Diseases / genetics; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Tobacco / genetics; Tobacco / metabolism
TL;DR: It is concluded that transgenic expression of CFP may have relevance for a disease control strategy in Cercospora-plant pathosystems where cercosporin is implicated in pathogen virulence. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Expression in sugar beet of the introduced cercosporin toxin export (CFP) gene from Cercospora kikuchii, the causative organism of purple seed stain in soybean

BIOTECHNOLOGY LETTERS, 26(9), 723–727.

By: L. Kuykendall* & R. Upchurch n

author keywords: Beta vulgaris; Cercospora beticola; leafspot disease; major facilitator; transgenic plant
MeSH headings : Agrobacterium tumefaciens / genetics; Ascomycota / genetics; Beta vulgaris / metabolism; Biotechnology / methods; Blotting, Western; DNA / metabolism; DNA Primers / chemistry; DNA Primers / genetics; Electrophoresis, Polyacrylamide Gel; Fungal Proteins / genetics; Gene Transfer Techniques; Genetic Vectors; Genotype; Membrane Transport Proteins / genetics; Plasmids / metabolism; Polymerase Chain Reaction; RNA / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Soybeans / microbiology; Time Factors; Transcription, Genetic; Transgenes
TL;DR: The Cercospora kikuchii cercosporin export gene, CFP, introduced into Beta vulgarisL by conjugation with Rhizobium radiobacter, was stably maintained during vegetative propagation as verified by PCR using primers specific for the CFP gene. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Transgenic assessment of CFP-mediated cercosporin export and resistance in a cercosporin-sensitive fungus

CURRENT GENETICS, 41(1), 25–30.

By: R. Upchurch n, M. Rose n, M. Eweida n & T. Callahan n

author keywords: cercosporin resistance; major facilitator; heterologous gene expression
MeSH headings : Antifungal Agents / pharmacology; Ascomycota / genetics; Ascomycota / metabolism; Carrier Proteins / genetics; Carrier Proteins / metabolism; Drug Resistance, Fungal / genetics; Fungal Proteins; Membrane Transport Proteins; Organisms, Genetically Modified; Perylene / analogs & derivatives; Perylene / pharmacology
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Over-expression of the cercosporin facilitator protein, CFP, in Cercospora kikuchii up-regulates production and secretion of cercosporin

FEMS MICROBIOLOGY LETTERS, 204(1), 89–93.

By: R. Upchurch n, M. Rose n & M. Eweida n

author keywords: cercosporin; CFP; major facilitator; over-expression; resistance; Cercospora kikuchii
MeSH headings : Ascomycota / drug effects; Ascomycota / genetics; Ascomycota / pathogenicity; Ascomycota / physiology; Carrier Proteins / genetics; Carrier Proteins / metabolism; Drug Resistance, Fungal; Enzyme Inhibitors / metabolism; Enzyme Inhibitors / pharmacology; Fungal Proteins; Gene Deletion; Gene Dosage; Gene Expression Regulation, Fungal; Immunoblotting; Membrane Transport Proteins; Perylene / analogs & derivatives; Perylene / metabolism; Perylene / pharmacology; Soybeans / microbiology; Transcription, Genetic; Up-Regulation
TL;DR: The three-fold increase in toxin accumulation, predominantly associated with the mycelium fraction of these CFP multi-copy strains, suggests that CFP may also have a significant, but unknown, role in regulating toxin production. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2000 patent

Fungal gene encoding resistance to the phytotoxin cercosporin

Washington, DC: U.S. Patent and Trademark Office.

By: R. Upchurch, T. Callahan & M. Ehrenshaft

Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

CFP, the putative cercosporin transporter of Cercospora kikuchii, is required for wild type cercosporin production, resistance, and virulence on soybean

MOLECULAR PLANT-MICROBE INTERACTIONS, 12(10), 901–910.

By: T. Callahan, M. Rose*, M. Meade, M. Ehrenshaft* & R. Upchurch*

author keywords: drug/proton antiporter
MeSH headings : Amino Acid Sequence; Ascomycota / genetics; Ascomycota / pathogenicity; Ascomycota / physiology; Base Sequence; Carrier Proteins / genetics; Carrier Proteins / metabolism; DNA, Fungal / genetics; Fungal Proteins / genetics; Fungal Proteins / metabolism; Genetic Complementation Test; Membrane Transport Proteins; Molecular Sequence Data; Open Reading Frames; Perylene / analogs & derivatives; Perylene / metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Soybeans / microbiology; Virulence
TL;DR: It is proposed that CFP (cercosporin facilitator protein) encodes a cercosporin transporter that contributes resistance to cercOSporin by actively exporting cercsporin, thus maintaining low cellular concentrations of the toxin. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1997 book

Current issues in symbiotic nitrogen fixation: Selected papers of the 15th North American Conference on Symbiotic Nitrogen Fixation held at North Carolina State University, Raleigh, NC, USA, 13-17 August 1995

Dordrecht; London: Kluwer Academic.

By: G. Elkan & R. Upchurch

Source: NC State University Libraries
Added: August 6, 2018

1995 journal article

Genetic regulation of cercosporin production in Cercospora kikuchii

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 72(12), 1435–1438.

By: R. Upchurch n

author keywords: Cercospora kikuchii; cercosporin; fungal pathogens; Glycine max; molecular genetics; mycotoxin; regulation; seed quality; soybean; weather damage
TL;DR: Knowing of the genetic and physiological regulation of cercosporin should provide new technological strategies for biocontrol of mycotoxins and the development of soybean breeding lines that exhibit durable resistance to C. kikuchii. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1994 journal article

Transformation of the fungal soybean pathogen Cercospora kikuchii with the selectable marker bar

Applied and Environmental Microbiology, 60(12), 4592.

By: R. Upchurch, M. Meade, R. Hightower, R. Thomas & T. Callahan

Source: NC State University Libraries
Added: August 6, 2018

1989 patent

Bradyrhizobium japonicum mutants exhibiting superior soybean nodulation

Washington, DC: U.S. Patent and Trademark Office.

By: R. Zablotowicz, R. Upchurch & J. Ligon

Source: NC State University Libraries
Added: August 6, 2018

1978 journal article

AMMONIA ASSIMILATION IN RHIZOBIUM-JAPONICUM COLONIAL DERIVATIVES DIFFERING IN NITROGEN-FIXING EFFICIENCY

JOURNAL OF GENERAL MICROBIOLOGY, 104(FEB), 219–225.

By: R. Upchurch* & G. Elkan

TL;DR: The idea that rhizobia can simultaneously derepress nitrogenase biosynthesis whilst repressing ammonia assimilatory enzyme biosynthesis is supported, i.e. those R. japonicum derivatives that fix greater amounts of nitrogen also assimilate less fixed nitrogen. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.

Certain data included herein are derived from the Web of Science© and InCites© (2024) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.