@article{hoffmann_flake_rossatto_de antonio_durigan_abreu_2024, title={Stem wound healing is dependent upon bark and trunk growth rates in Brazilian savanna}, ISSN={["1432-2285"]}, DOI={10.1007/s00468-023-02477-3}, journal={TREES-STRUCTURE AND FUNCTION}, author={Hoffmann, William A. and Flake, Samuel W. and Rossatto, Davi R. and De Antonio, Ariadne C. and Durigan, Giselda and Abreu, Rodolfo C. R.}, year={2024}, month={Jan} }
 @article{gold_pellegrini_refsland_andrioli_bowles_brockway_burrows_franco_hallgren_hobbie_et al._2023, title={Herbaceous vegetation responses to experimental fire in savannas and forests depend on biome and climate}, ISSN={["1461-0248"]}, DOI={10.1111/ele.14236}, abstractNote={Fire-vegetation feedbacks potentially maintain global savanna and forest distributions. Accordingly, vegetation in savanna and forest ecosystems should have differential responses to fire, but fire response data for herbaceous vegetation have yet to be synthesized across biomes. Here, we examined herbaceous vegetation responses to experimental fire at 30 sites spanning four continents. Across a variety of metrics, herbaceous vegetation increased in abundance where fire was applied, with larger responses to fire in wetter and in cooler and/or less seasonal systems. Compared to forests, savannas were associated with a 4.8 (±0.4) times larger difference in herbaceous vegetation abundance for burned versus unburned plots. In particular, grass cover decreased with fire exclusion in savannas, largely via decreases in C4 grass cover, whereas changes in fire frequency had a relatively weak effect on grass cover in forests. These differential responses underscore the importance of fire for maintaining the vegetation structure of savannas and forests.}, journal={ECOLOGY LETTERS}, author={Gold, Zachary J. and Pellegrini, Adam F. A. and Refsland, Tyler K. and Andrioli, Romina J. and Bowles, Marlin L. and Brockway, Dale G. and Burrows, Neil and Franco, Augusto C. and Hallgren, Steve W. and Hobbie, Sarah E. and et al.}, year={2023}, month={May} }
 @article{lima pilon_cava_hoffmann_abreu_rossatto_durigan_2022, title={Effects and response of the Cerrado ground-layer to frost along the canopy cover gradient}, ISSN={["1432-1939"]}, DOI={10.1007/s00442-022-05259-9}, abstractNote={Frost effects on savanna plant communities have been considered as analogous to those from fire, both changing community structure and filtering species composition. However, while frost impacts have been well-studied for the woody component of savannas, it is still poorly explored for the ground-layer community. Here, we investigated effects of frost in the Cerrado along a gradient of tree cover, focusing on ground-layer plant species, near the southern limit of the Cerrado in Brazil. We aimed to elucidate if the pattern already described for the tree layer also extends to the ground layer in terms of mimicking the effects of fire on vegetation structure and composition. We assessed how damage severity differs across species and across the tree-cover gradient, and we examined the recovery process after frost in terms of richness and community structure along the canopy cover gradient. Frost caused immediate and widespread dieback of the perennial ground-layer, with greatest impact on community structure where tree cover was lowest. However, frost did not reduce the number of species, indicating community resilience to this natural disturbance. Although frost mimicked the effects of fire in some ways, in other ways it differed substantially from fire. Unlike fire, frost increases litter cover and decreases the proportion of bare soil, likely hindering crucial processes for recovery of plant populations, such as seed dispersal, seed germination and plant resprouting. This finding calls attention to the risk of misguided conclusions when the ground layer is neglected in ecological studies of tropical savannas and grasslands.}, journal={OECOLOGIA}, author={Lima Pilon, Natashi A. and Cava, Mario G. B. and Hoffmann, William A. and Abreu, Rodolfo C. R. and Rossatto, Davi R. and Durigan, Giselda}, year={2022}, month={Sep} }
 @article{flake_honda_pilon_hoffmann_durigan_2022, title={Not all trees can make a forest: Tree species composition and competition control forest encroachment in a tropical savanna}, ISSN={["1365-2745"]}, DOI={10.1111/1365-2745.13820}, abstractNote={Forest encroachment into savannas is a widespread phenomenon, the rate of which may depend on soil conditions, species composition or changes in stand structure. As savanna specialist trees are replaced by generalist species, rates of stand development may increase. Because generalists can persist in forests, they are likely to grow more quickly and survive longer in dense stands, compared to savanna specialists. Furthermore, the faster growth rates of generalists may allow them to overtop and outcompete savanna specialists, causing rapid species turnover. We measured growth and survival of 6,147 individuals of 112 species of savanna and generalist tree species over a period of 10 years in an ecological reserve in Assis, São Paulo State, Brazil. We modelled growth and mortality as a function of soil texture and nutrients, tree size, competitive neighbourhood, and membership in savanna or generalist (species which can persist in forests and savannas) functional groups. Tree growth and survival was strongly influenced by competition, as estimated by the basal area of trees taller than a focal tree. At the stand level, savanna species are unable to contribute basal area growth in closed stands, while generalist species continue to increase in basal area even at high stand basal area. This phenomenon is driven by differences in growth and mortality. Generalists grew faster than savanna species, both in height and diameter. This difference in growth rates led to savanna species becoming suppressed more rapidly than generalists. When suppressed, savanna species were more than twice as likely to die than were generalists. Soils had inconsistent and mostly weak effects which were difficult to separate from gradients of stand structure. Synthesis. We demonstrate that the presence of generalist trees accelerates the rates of basal area accumulation due to their greater growth rates and tolerance of shading. Generalists outcompete savanna trees by growing faster in the open and overtopping savanna specialists. Due to the slow growth and high mortality of savanna species in the shade, they are unable to form closed‐canopy stands. Accounting for differences among functional types and development of vegetation structure is critical for modelling forest encroachment.}, journal={JOURNAL OF ECOLOGY}, author={Flake, Samuel W. and Honda, Eliane A. and Pilon, Natashi A. L. and Hoffmann, William A. and Durigan, Giselda}, year={2022}, month={Jan} }
 @article{wall_hohmann_just_hoffmann_2021, title={Characterizing past fire occurrence in longleaf pine ecosystems with the Mid-Infrared Burn Index and a Random Forest classifier}, volume={500}, ISSN={["1872-7042"]}, DOI={10.1016/j.foreco.2021.119635}, abstractNote={Prior to European settlement the longleaf pine (Pinus palustris) ecosystem covered over 92 million hectares in the southeastern United States. Historically, fire was an important driver of species composition in the longleaf pine ecosystem, but fire exclusion since the early 20th century has led to the degradation of longleaf pine communities and has had a detrimental effect on the large number of rare and endemic species found within this system. Thus, accurate estimates of fire history are important for better informed management of longleaf pine communities. Recently, satellite imagery has been used to identify burned areas. However, results have been inconsistent across physiographic regions and vegetation types (e.g. wetlands under high canopy). We developed a model using Landsat satellite imagery, coupled with a Random Forest (RF) machine learning algorithm, to identify burned areas and estimate the fire history from 1991 to 2019 for Fort Bragg, NC, one of the largest contiguous areas of longleaf pine ecosystem remaining. We calculated six spectral indices from the Landsat band values, including the Mid-Infrared Burn Index (MIRBI) and the change in MIRBI through time (ΔMIRBI), and used them as predictors in our RF model. We used the developed RF model to estimate the fire history for all known populations of 24 rare upland and wetland plant species found on Fort Bragg. We compared our results to a recent continental U.S. fire occurrence dataset, as well as the prescribed fire records from Fort Bragg. The overall AUC (area under the curve) for our RF model (0.74) compared favorably to the continental U.S. dataset results for Fort Bragg (0.69), and was able to capture the reduced fire frequency in wetlands. The most important predictor in our RF model was ΔMIRBI. Depending on the model, individual plant species were estimated to have experienced significant differences in fire frequency relative to the prescribed fire records. For our RF model, we estimated that 50% of wetland and 25% of upland species experienced a lower fire frequency relative to that represented in the prescribed fire records. The burn probability and classification tool generated in this paper provides land managers in the southeastern U.S. with a novel approach for accurately identifying burned areas and estimating local fire frequency across landscapes.}, journal={FOREST ECOLOGY AND MANAGEMENT}, author={Wall, Wade A. and Hohmann, Matthew G. and Just, Michael G. and Hoffmann, William A.}, year={2021}, month={Nov} }
 @article{pellegrini_refsland_averill_terrer_staver_brockway_caprio_clatterbuck_coetsee_haywood_et al._2021, title={Decadal changes in fire frequencies shift tree communities and functional traits}, volume={5}, ISSN={["2397-334X"]}, DOI={10.1038/s41559-021-01401-7}, abstractNote={Global change has resulted in chronic shifts in fire regimes. Variability in the sensitivity of tree communities to multi-decadal changes in fire regimes is critical to anticipating shifts in ecosystem structure and function, yet remains poorly understood. Here, we address the overall effects of fire on tree communities and the factors controlling their sensitivity in 29 sites that experienced multi-decadal alterations in fire frequencies in savanna and forest ecosystems across tropical and temperate regions. Fire had a strong overall effect on tree communities, with an average fire frequency (one fire every three years) reducing stem density by 48% and basal area by 53% after 50 years, relative to unburned plots. The largest changes occurred in savanna ecosystems and in sites with strong wet seasons or strong dry seasons, pointing to fire characteristics and species composition as important. Analyses of functional traits highlighted the impact of fire-driven changes in soil nutrients because frequent burning favoured trees with low biomass nitrogen and phosphorus content, and with more efficient nitrogen acquisition through ectomycorrhizal symbioses. Taken together, the response of trees to altered fire frequencies depends both on climatic and vegetation determinants of fire behaviour and tree growth, and the coupling between fire-driven nutrient losses and plant traits. Using tree community data from 29 tropical and temperate sites that have experienced multi-decadal alterations in fire frequency, the authors show repeated burning generally reduces stem density and basal area, with most pronounced effects in savanna ecosystems and in sites with strong wet seasons or strong dry seasons.}, number={4}, journal={NATURE ECOLOGY & EVOLUTION}, author={Pellegrini, Adam F. A. and Refsland, Tyler and Averill, Colin and Terrer, Cesar and Staver, A. Carla and Brockway, Dale G. and Caprio, Anthony and Clatterbuck, Wayne and Coetsee, Corli and Haywood, James D. and et al.}, year={2021}, month={Apr}, pages={504-+} }
 @article{abreu_durigan_melo_pilon_hoffmann_2021, title={Facilitation by isolated trees triggers woody encroachment and a biome shift at the savanna-forest transition}, ISSN={["1365-2664"]}, DOI={10.1111/1365-2664.13994}, abstractNote={Abstract Woody encroachment into grassy biomes is a global phenomenon, often resulting in a nearly complete turnover of species, with savanna specialists being replaced by forest‐adapted species. Understanding the mechanisms involved in this change is important for devising strategies for managing savannas. We examined how isolated trees favour woody encroachment and species turnover by overcoming dispersal limitation and environmental filtering. In a savanna released from fire in south‐eastern Brazil (Cerrado), we sampled woody plants establishing under 40 tree canopies and in paired treeless plots. These trees comprised eight species selected for habitat preference (savanna or forest) and dispersal syndrome (bird dispersed or not). We recorded dimensions of each tree, dispersal syndrome and habitat preference of recruits, and quantified the physical environment within each plot, aiming at a mechanistic understanding of woody encroachment. We found clear evidence that isolated trees cause nucleation and drive changes in functional composition of savanna. Effectiveness as nucleator differed among species, but was unrelated to their functional guilds (habitat preference or dispersal syndrome). The density of saplings in nuclei was partially explained by soil moisture (+), daily temperature amplitude (−) and sum of bases (−). Our results indicate that isolated trees act first as perches, strongly favouring bird‐dispersed species. They then act as nurse trees, considerably changing the environment in favour of forest‐adapted recruits. In the long term, as the nuclei expand and merge, savanna specialists tend to disappear and the savanna turns into a low‐diversity forest. Synthesis and applications . Fire suppression has allowed the nucleation process and consequently the woody encroachment and fast replacement of savanna specialists by forest species in the Cerrado. By elucidating the mechanisms behind woody encroachment, we recommend using prescribed fires to burn forest seedlings and to reduce tree canopy size wherever the management goal is to maintain the typical savanna structure and composition.}, journal={JOURNAL OF APPLIED ECOLOGY}, author={Abreu, Rodolfo C. R. and Durigan, Giselda and Melo, Antonio C. G. and Pilon, Natashi A. L. and Hoffmann, William A.}, year={2021}, month={Aug} }
 @article{scalon_rossatto_oliveras_miatto_gray_chaves bicalho domingos_brum_carlucci_hoffmann_marimon-junior_et al._2021, title={Fire and drought: Shifts in bark investment across a broad geographical scale for Neotropical savanna trees}, volume={56}, ISSN={["1618-0089"]}, DOI={10.1016/j.baae.2021.06.011}, abstractNote={Savanna tree communities occurring in confluence zones with other biomes likely experience different environmental pressures, resulting in shifts in the selection of individual traits, the combinations of such traits, and species composition. In seasonally dry fire-prone environments, plant survival is presumably associated with adaptive changes in bark properties related to fire protection and water storage. Here, we integrated the multiple functions of the bark to investigate whether different selective pressures could influence patterns of variation in bark structure and allocation across species in a broad geographical range. We measured thickness, density, and water content of the inner and outer bark in branches and the main stem of the 51 most abundant species in three savanna communities differing in climatic aridity, one located at the core region of Cerrado in Central Brazil and the other two at its periphery, in the transition zones with Amazonia and Atlantic forest biomes. We found no difference in outer bark thickness but markedly difference in inner bark thickness between the three plant communities. In the central region, where dry season is long and fire is frequent, branches and main stem showed thicker inner bark. Contrastingly, in the south periphery region, where dry season is short, species showed thinner inner bark in both branches and main stem. Species from the north periphery region, where mean annual precipitation is higher, but fire is frequent and the dry season is also long, showed similar main stem inner bark thickness, but thinner branch inner bark compared to core region species. Our findings support the idea that investing in inner bark thickness and bark moisture may be the most advantageous strategy in plant communities that suffer from high evaporative demand during a long period and are at a high risk of fire.}, journal={BASIC AND APPLIED ECOLOGY}, author={Scalon, Marina Correa and Rossatto, Davi Rodrigo and Oliveras, Imma and Miatto, Raquel Carolina and Gray, Emma Fiona and Chaves Bicalho Domingos, Fabricius Maia and Brum, Fernanda Thiesen and Carlucci, Marcos Bergmann and Hoffmann, William Arthur and Marimon-Junior, Ben Hur and et al.}, year={2021}, month={Nov}, pages={110–121} }
 @article{hoffmann_rodrigues_uncles_rossi_2021, title={Hydraulic segmentation does not protect stems from acute water loss during fire}, volume={41}, ISSN={["1758-4469"]}, DOI={10.1093/treephys/tpab057}, abstractNote={The heat plume associated with fire has been hypothesized to cause sufficient water loss from trees to induce embolism and hydraulic failure. However, it is unclear whether the water transport path remains sufficiently intact during scorching or burning of foliage to sustain high water loss. We measured water uptake by branches of Magnolia grandiflora while exposing them to a range of fire intensities, and examined factors influencing continued water uptake after fire. Burning caused a 22-fold mean increase in water uptake, with greatest rates of water loss observed at burn intensities that caused complete consumption of leaves. Such rapid uptake is possible only with steep gradients in water potential, which would likely result in substantial cavitation of xylem and loss of conductivity in intact stems. Water uptake continued after burning was complete, and was greatest following burn intensities that killed leaves but did not consume them. This post-fire uptake was mostly driven by rehydration of the remaining tissues, rather than evaporation from the tissues. Our results indicate that the fire-plume hypothesis can be expanded to include a wide range of burning conditions experienced by plants. High rates of water loss are sustained during burning, even when leaves are killed or completely consumed.}, number={10}, journal={TREE PHYSIOLOGY}, author={Hoffmann, William A. and Rodrigues, Amanda C. and Uncles, Nicholas and Rossi, Lorenzo}, year={2021}, month={Oct}, pages={1785–1793} }
 @article{flake_abreu_durigan_hoffmann_2021, title={Savannas are not old fields: Functional trajectories of forest expansion in a fire-suppressed Brazilian savanna are driven by habitat generalists}, ISSN={["1365-2435"]}, DOI={10.1111/1365-2435.13818}, abstractNote={Abstract Under fire suppression, many tropical savannas transform into forests. Forest expansion entails changes in environmental variables and plant community structure. We hypothesized that forest expansion into savanna results in a shift in community‐weighted mean functional traits from stress tolerance to competitiveness, with generalist species having trait values intermediate between those of specialists of savanna and forest habitats. We studied 30 plots distributed over three savanna–forest boundaries undergoing forest expansion in the Brazilian Cerrado, capturing a gradient from open savanna to recently formed forest. We measured functional traits of 116 woody species of savanna specialist, generalist and forest specialist functional groups and quantified changes in species composition and mean traits across the basal area gradient. We identified two main axes of species traits. The first separated forest and generalist species from savanna specialists, with the latter possessing traits associated with resistance to disturbance and stress— such as thick leaves, thick bark, slower height growth and lower shade tolerance. Our second trait axis separated shrubs and understorey trees from pioneer species. Generalist species’ traits did not differ substantially from forest species, nor did they tend to have a typical pioneer strategy. Community‐weighted trait means changed linearly with forest development. There was a steady increase in traits associated with competitive dominance rather than stress tolerance and fire resistance, indicating a wholesale shift in the selective environment. Several of these patterns—for example, increasing height and decreasing light requirements—are common in old‐field succession. In contrast to old‐field succession, we found that SLA increased, leaf thickness decreased and wood density stayed constant. The assembly of forests appears to be shaped by environmental filters that contribute to a functional trajectory distinct from most other studied ecosystems. Our results highlight the importance of the functional composition of the early community and of the early colonizers of the open environment. Differences between savanna and forest specialists reflect the selective effects of the contrasting environments, while the traits of generalists—and their interaction with environmental filters—drive the dynamics of forest expansion. A free Plain Language Summary can be found within the Supporting Information of this article.}, journal={FUNCTIONAL ECOLOGY}, author={Flake, Samuel W. and Abreu, Rodolfo C. R. and Durigan, Giselda and Hoffmann, William A.}, year={2021}, month={May} }
 @article{pilon_durigan_rickenback_pennington_dexter_hoffmann_abreu_lehmann_2021, title={Shade alters savanna grass layer structure and function along a gradient of canopy cover}, volume={32}, ISSN={["1654-1103"]}, DOI={10.1111/jvs.12959}, abstractNote={Abstract}, number={1}, journal={JOURNAL OF VEGETATION SCIENCE}, author={Pilon, Natashi A. L. and Durigan, Giselda and Rickenback, Jess and Pennington, R. Toby and Dexter, Kyle G. and Hoffmann, William A. and Abreu, Rodolfo C. R. and Lehmann, Caroline E. R.}, year={2021}, month={Jan} }
 @article{pilon_cava_hoffmann_abreu_fidelis_durigan_2021, title={The diversity of post-fire regeneration strategies in the cerrado ground layer}, volume={109}, ISSN={["1365-2745"]}, DOI={10.1111/1365-2745.13456}, abstractNote={Disentangling species strategies that confer resilience to natural disturbances is key to conserving and restoring savanna ecosystems. Fire is a recurrent disturbance in savannas, and savanna vegetation is highly adapted to and often dependent on fire. However, although the woody component of tropical savannas is well studied, we still do not understand how ground‐layer plant communities respond to fire, limiting conservation and management actions. We investigated the effects of prescribed fire on community structure and composition, and evaluated which traits are involved in plant community regeneration after fire in the cerrado ground layer. We assessed traits related to species persistence and colonization capacity after fire, including resprouter type, underground structure, fire‐induced flowering, regeneration strategy and growth form. We searched for functional groups related to response to fire, to shed light on the main strategies of post‐fire recovery among species in the ground layer. Fire changed ground‐layer community structure and composition in the short term, leading to greater plant species richness, population densities and increasing bare soil, compared with unburned communities. Eight months after fire, species abundance did not differ from pre‐disturbance values for 86% of the species, demonstrating the resilience of this layer to fire. Only one ruderal species was disadvantaged by fire and 13% of the species benefited. Rapid recovery of soil cover by native vegetation in burned areas was driven by species with high capacity to resprout and spread vegetatively. Recovery of the savanna ground‐layer community, as a whole, resulted from a combination of different species traits. We summarized these traits into five large groups, encompassing key strategies involved in ground‐layer regeneration after fire. Synthesis. Fire dramatically changes the ground layer of savanna vegetation in the short term, but the system is highly resilient, quickly recovering the pre‐fire state. Recovery involves different strategies, which we categorized into five functional groups of plant species: grasses, seeders, bloomers, undergrounders and resprouters. Knowledge of these diverse strategies should be used as a tool to assess conservation and restoration status of fire‐resilient ecosystems in the cerrado.}, number={1}, journal={JOURNAL OF ECOLOGY}, author={Pilon, Natashi A. L. and Cava, Mario G. B. and Hoffmann, William A. and Abreu, Rodolfo C. R. and Fidelis, Alessandra and Durigan, Giselda}, year={2021}, month={Jan}, pages={154–166} }
 @article{massi_orlando eugenio_franco_hoffmann_2021, title={The effects of tree cover and soil nutrient addition on native herbaceous richness in a neotropical savanna}, volume={53}, ISSN={["1744-7429"]}, DOI={10.1111/btp.12940}, abstractNote={Exotic grasses and high‐nutrient availability are common factors that may limit recovery of herbaceous diversity in derived savannas, while tree encroachment is a threat to diversity in old‐growth savannas. To understand the impacts of these factors on herbaceous communities, we studied the effect of nutrient addition, and the resulting increase in the exotic grass, Melinis minutiflora, at a savanna‐forest boundary in the Brazilian Cerrado. We inventoried richness of reproductive herbs, tree basal area and cover of the exotic grass, Melinis minutiflora, in each plot. Nutrient addition caused a large increase in Melinis and a large decrease in richness of flowering plants. Unexpectedly, structural equation model analysis suggests that the decline in herbaceous richness was a direct result of nutrient addition, rather than an indirect effect caused by the increase in Melinis. Tree density had a strong negative effect on both Melinis cover and herbaceous richness. Our results reveal that high‐nutrient availability imposes a barrier to the restoration of a diverse, native herbaceous layer in anthropogenic savannas, while tree encroachment is a threat to diversity in old‐growth savannas.}, number={3}, journal={BIOTROPICA}, author={Massi, Klecia Gili and Orlando Eugenio, Chesterton Ulysses and Franco, Augusto Cesar and Hoffmann, William A.}, year={2021}, month={May}, pages={888–895} }
 @article{de antonio_hoffmann_rossatto_2021, title={The role of morpho-physiological traits in frost tolerance of neotropical savanna trees}, ISSN={["1432-2285"]}, DOI={10.1007/s00468-021-02150-7}, journal={TREES-STRUCTURE AND FUNCTION}, author={De Antonio, Ariadne C. and Hoffmann, William A. and Rossatto, Davi R.}, year={2021}, month={Jun} }
 @article{hoffmann_sanders_just_wall_hohmann_2020, title={Better lucky than good: How savanna trees escape the fire trap in a variable world}, volume={101}, ISSN={["1939-9170"]}, DOI={10.1002/ecy.2895}, abstractNote={Abstract}, number={1}, journal={ECOLOGY}, author={Hoffmann, William A. and Sanders, R. Wyatt and Just, Michael G. and Wall, Wade A. and Hohmann, Matthew G.}, year={2020}, month={Jan} }
 @article{newberry_power_abreu_durigan_rossatto_hoffmann_2020, title={Flammability thresholds or flammability gradients? Determinants of fire across savanna-forest transitions}, volume={228}, ISSN={["1469-8137"]}, DOI={10.1111/nph.16742}, abstractNote={Vegetation-fire feedbacks are important for determining the distribution of forest and savanna. To understand how vegetation structure controls these feedbacks, we quantified flammability across gradients of tree density from grassland to forest in the Brazilian Cerrado. We experimentally burned 102 plots, for which we measured vegetation structure, fuels, microclimate, ignition success and fire behavior. Tree density had strong negative effects on ignition success, rate of spread, fire-line intensity and flame height. Declining grass biomass was the principal cause of this decline in flammability as tree density increased, but increasing fuel moisture contributed. Although the response of flammability to tree cover often is portrayed as an abrupt, largely invariant threshold, we found the response to be gradual, with considerable variability driven largely by temporal changes in atmospheric humidity. Even when accounting for humidity, flammability at intermediate tree densities cannot be predicted reliably. Fire spread in savanna-forest mosaics is not as deterministic as often assumed, but may appear so where vegetation boundaries are already sharp. Where transitions are diffuse, fire spread is difficult to predict, but should become increasingly predictable over multiple fire cycles, as boundaries are progressively sharpened until flammability appears to respond in a threshold-like manner.}, number={3}, journal={NEW PHYTOLOGIST}, author={Newberry, Brooklynn M. and Power, Collin R. and Abreu, Rodolfo C. R. and Durigan, Giselda and Rossatto, Davi R. and Hoffmann, William A.}, year={2020}, month={Nov}, pages={910–921} }
 @article{durigan_pilon_abreu_hoffmann_martins_fiorillo_antunes_carmignotto_maravalhas_vieira_et al._2020, title={No Net Loss of Species Diversity After Prescribed Fires in the Brazilian Savanna}, volume={3}, ISSN={["2624-893X"]}, DOI={10.3389/ffgc.2020.00013}, abstractNote={Although savannas are fire-adapted ecosystems, prescribing fire for biodiversity conservation remains controversial at least in some regions where savannas occur. Faced with uncertainty, many decision makers and even scientists are still reluctant to prescribe fire for conservation purposes in fire-prone ecosystems, invoking the precautionary principle. Knowledge gaps on the ideal fire regime, such as how and when to burn, and especially the fear of biodiversity losses, are among the main arguments against fire management applied to remnants of native savanna vegetation. To inform this debate, we assessed the impact of prescribed fires on diversity of plants (different growth forms), ants, frogs, lizards, birds, and small mammals, in savannas and grasslands of the Brazilian Cerrado. We assessed the existing species richness, composition, and abundance in areas subjected to long periods of fire suppression and compared to that observed over a short period after prescribed dry-season fires, within each group of plants and animals. Whenever possible, we carried out separate analyses for grassland and savanna. Burning did not significantly reduce species richness of any of the groups analyzed, but had a positive effect on richness of graminoids in grassland. When analyzed at the species level, abundance of most animal groups did not show consistent responses to fire, except for a decrease in some frog populations in grasslands. Forbs, graminoids, and subshrubs increased in abundance after fire in grassland areas, though in savanna areas, abundance of forbs, and subshrubs tended to decline after fire. Species composition changed little in response to fire as indicated by low levels of dissimilarity between burned and unburned areas. These results confirm the high resilience of Cerrado biota to fire, as expected for savanna ecosystems in general. Besides, we demonstrate here that the risk of biodiversity losses cannot justify the objections to the use of prescribed fire for conservation purposes in the Cerrado.}, journal={FRONTIERS IN FORESTS AND GLOBAL CHANGE}, author={Durigan, Giselda and Pilon, Natashi A. L. and Abreu, Rodolfo C. R. and Hoffmann, William A. and Martins, Marcio and Fiorillo, Bruno F. and Antunes, Alexsander Z. and Carmignotto, Ana Paula and Maravalhas, Jonas B. and Vieira, Jesica and et al.}, year={2020}, month={Feb} }
 @article{gotsch_geiger_franco_goldstein_meinzer_hoffmann_2019, title={Allocation to leaf area and sapwood area affects water relations of co-occurring savanna and forest trees (vol 163, pg 291, 2010)}, volume={189}, ISSN={["1432-1939"]}, DOI={10.1007/s00442-018-04327-3}, abstractNote={The original version of this article unfortunately contained a mistake. The Electronic supplementary material (ESM) was accompanying this article by mistake.}, number={2}, journal={OECOLOGIA}, author={Gotsch, Sybil G. and Geiger, Erika L. and Franco, Augusto C. and Goldstein, Guillermo and Meinzer, Frederick C. and Hoffmann, William A.}, year={2019}, month={Feb}, pages={563–563} }
 @article{veldman_aleman_alvarado_anderson_archibald_bond_boutton_buchmann_buisson_canadell_et al._2019, title={Comment on "The global tree restoration potential"}, volume={366}, ISSN={["1095-9203"]}, DOI={10.1126/science.aay7976}, abstractNote={
            Bastin
            et al
            .’s estimate (Reports, 5 July 2019, p. 76) that tree planting for climate change mitigation could sequester 205 gigatonnes of carbon is approximately five times too large. Their analysis inflated soil organic carbon gains, failed to safeguard against warming from trees at high latitudes and elevations, and considered afforestation of savannas, grasslands, and shrublands to be restoration.
          }, number={6463}, journal={SCIENCE}, author={Veldman, Joseph W. and Aleman, Julie C. and Alvarado, Swanni T. and Anderson, T. Michael and Archibald, Sally and Bond, William J. and Boutton, Thomas W. and Buchmann, Nina and Buisson, Elise and Canadell, Josep G. and et al.}, year={2019}, month={Oct} }
 @article{hoffmann_flake_abreu_pilon_rossatto_durigan_2019, title={Rare frost events reinforce tropical savanna-forest boundaries}, volume={107}, ISSN={["1365-2745"]}, DOI={10.1111/1365-2745.13047}, abstractNote={Abstract}, number={1}, journal={JOURNAL OF ECOLOGY}, author={Hoffmann, William A. and Flake, Samuel W. and Abreu, Rodolfo C. R. and Pilon, Natashi A. L. and Rossatto, Davi R. and Durigan, Giselda}, year={2019}, month={Jan}, pages={468–477} }
 @article{pilon_hoffmann_abreu_durigan_2018, title={Quantifying the short-term flowering after fire in some plant communities of a cerrado grassland}, volume={11}, ISSN={["1755-1668"]}, DOI={10.1080/17550874.2018.1517396}, abstractNote={ABSTRACT Background: Fire has been reported to trigger the production of flowers and fruits in many fire-prone ecosystems around the world. However, for tropical savannas, little is known about the effects of fire on flower production at community and species scale, especially for the ground-layer. Aims: We assessed the role of fire as a trigger to short-term flowering in cerrado grassland, compared with unburned vegetation. Methods: We recorded the presence of flowers or fruits in 2,441 individuals from 47 plant species (grasses, forbs and subshrubs), during 6 months after fire, in burned and unburned areas of cerrado grasslands, in south-eastern Brazil. Results: In the burned areas, 63% of individuals sampled flowered, in contrast to 19% in unburned areas, demonstrating a strong and positive effect of fire on plant communities of cerrado grasslands. Fire significantly induced flowering in 79% of the studied species, of which 20 species flowered only after fire (nine grasses, seven subshrubs and four forbs). Conclusions: These results highlight the role of fire triggering important ecological processes in the cerrado grasslands, potentially benefiting seed production and genetic diversity of many species. Fire is a crucial factor to be maintained for conservation of these ecosystems and their biodiversity.}, number={3}, journal={PLANT ECOLOGY & DIVERSITY}, author={Pilon, Natashi A. L. and Hoffmann, William A. and Abreu, Rodolfo C. R. and Durigan, Giselda}, year={2018}, pages={259–266} }
 @article{trugman_medvigy_hoffmann_pellegrini_2018, title={Sensitivity of woody carbon stocks to bark investment strategy in Neotropical savannas and forests}, volume={15}, ISSN={["1726-4189"]}, DOI={10.5194/bg-15-233-2018}, abstractNote={Abstract. Fire frequencies are changing in Neotropical savannas and
forests as a result of forest fragmentation and increasing drought. Such
changes in fire regime and climate are hypothesized to destabilize tropical
carbon storage, but there has been little consideration of the widespread
variability in tree fire tolerance strategies. To test how aboveground
carbon stocks change with fire frequency and composition of plants with
different fire tolerance strategies, we update the Ecosystem Demography
model 2 (ED2) with (i) a fire survivorship module based on tree bark
thickness (a key fire-tolerance trait across woody plants in savannas and
forests), and (ii) plant functional types representative of trees in the
region. With these updates, the model is better able to predict how fire
frequency affects population demography and aboveground woody carbon.
Simulations illustrate that the high survival rate of thick-barked, large
trees reduces carbon losses with increasing fire frequency, with high
investment in bark being particularly important in reducing losses in the
wettest sites. Additionally, in landscapes that frequently burn, bark
investment can broaden the range of climate and fire conditions under which
savannas occur by reducing the range of conditions leading to either
complete tree loss or complete grass loss. These results highlight that
tropical vegetation dynamics depend not only on rainfall and changing fire
frequencies but also on tree fire survival strategy. Further, our results
indicate that fire survival strategy is fundamentally important in
regulating tree size demography in ecosystems exposed to fire, which
increases the preservation of aboveground carbon stocks and the coexistence
of different plant functional groups.
                    }, number={1}, journal={BIOGEOSCIENCES}, author={Trugman, Anna T. and Medvigy, David and Hoffmann, William A. and Pellegrini, Adam F. A.}, year={2018}, month={Jan}, pages={233–243} }
 @article{bagherzadi_sinclair_zwieniecki_secchi_hoffmann_carter_rufty_2017, title={Assessing, water-related plant traits to explain slow-wilting in soybean PI 471938}, volume={31}, ISSN={["1542-7536"]}, DOI={10.1080/15427528.2017.1309609}, abstractNote={ABSTRACT Soybean [Glycine max (L.) Merr.] genotype PI 471938 expresses a slow-wilting phenotype in the field, and the progeny of this genotype have shown to have high yield under water deficit conditions. However, the physiological basis for the slow-wilting trait in PI 471938 remains unclear, and failure to understand the causal mechanism may limit future breeding efforts. This study investigated three primary hypotheses for trait expression that could explain slow-wilting trait in PI 471938: (1) a low osmotic potential in the leaves allowing greater water retention, (2) high elastic modulus of leaves resulting in delayed development of wilting, and (3) high hydraulic conductance allowing rapid water redistribution in the plants. Experiments included three other soybean genotypes as references for the results obtained with PI 471938. Surprisingly, the results for PI 471938 did not prove to be unique as compared to the other three tested genotypes for any of the three hypotheses. These negative results indicate that a hypothesis outside the usual candidates describing plant water transport, possibly anatomical features related to specific water transport properties, is required to explain slow-wilting in PI 471938.}, number={3}, journal={JOURNAL OF CROP IMPROVEMENT}, author={Bagherzadi, Laleh and Sinclair, Thomas R. and Zwieniecki, Maciej and Secchi, Francesca and Hoffmann, William and Carter, Thomas E. and Rufty, Thomas W.}, year={2017}, pages={400–417} }
 @article{griffith_lehmann_stromberg_parr_pennington_sankaran_ratnam_still_powell_hanan_et al._2017, title={Comment on "The extent of forest in dryland biomes"}, volume={358}, number={6365}, journal={Science}, author={Griffith, D. M. and Lehmann, C. E. R. and Stromberg, C. A. E. and Parr, C. L. and Pennington, R. T. and Sankaran, M. and Ratnam, J. and Still, C. J. and Powell, R. L. and Hanan, N. P. and et al.}, year={2017} }
 @article{pellegrini_anderegg_paine_hoffmann_kartzinel_rabin_sheil_franco_pacala_2017, title={Convergence of bark investment according to fire and climate structures ecosystem vulnerability to future change}, volume={20}, ISSN={["1461-0248"]}, DOI={10.1111/ele.12725}, abstractNote={Abstract}, number={3}, journal={ECOLOGY LETTERS}, author={Pellegrini, Adam F. A. and Anderegg, William R. L. and Paine, C. E. Timothy and Hoffmann, William A. and Kartzinel, Tyler and Rabin, Sam S. and Sheil, Douglas and Franco, Augusto C. and Pacala, Stephen W.}, year={2017}, month={Mar}, pages={307–316} }
 @article{just_hohmann_hoffmann_2017, title={Invasibility of a fire-maintained savanna-wetland gradient by non-native, woody plant species}, volume={405}, ISSN={["1872-7042"]}, DOI={10.1016/j.foreco.2017.09.052}, abstractNote={Fire-promoting, open-canopy ecosystems are under threat of conversion to a fire-deterring, closed-canopy condition due to woody encroachment. This conversion of vegetation structure has been fostered by introduced woody plant species. We performed a field experiment to quantify growth, survival, and establishment success of six invasive, woody species along a managed longleaf pine savanna–wetland gradient in the Sandhills of North Carolina, USA. We investigated the effects of prescribed fire, fire history, dispersal, and abiotic conditions on the invasibility of sites along the gradient. Across 18 study sites, seeds of the six woody species were sown using three sowing methods that mimicked primary and secondary dispersal; each site contained paired plots located in savanna and savanna-wetland ecotone vegetation communities. We identified sowing treatment, abiotic conditions, seedling size, and prescribed fire as important factors for controlling woody invasion, as they prevented 5 of 6 study species from establishing in the landscape. However, the landscape was not immune to invasion. At the end of the 42-month study period, three species had established in unburned sites. In sites burned after seedling emergence, only one species, Pyrus calleryana, survived and established. We found P. calleryana survival and establishment to be a function of seedling size, soil humic matter content, and sowing treatment. Successful invasion and establishment of woody individuals in open-canopied systems increases the likelihood of fire-deterrence and further woody encroachment, threatening ecosystem integrity.}, journal={FOREST ECOLOGY AND MANAGEMENT}, author={Just, Michael G. and Hohmann, Matthew G. and Hoffmann, William A.}, year={2017}, month={Dec}, pages={229–237} }
 @article{abreu_hoffmann_vasconcelos_pilon_rossatto_durigan_2017, title={The biodiversity cost of carbon sequestration in tropical savanna}, volume={3}, ISSN={["2375-2548"]}, DOI={10.1126/sciadv.1701284}, abstractNote={Forest expansion into Brazilian savanna due to fire suppression causes precipitous species loss.}, number={8}, journal={SCIENCE ADVANCES}, author={Abreu, Rodolfo C. R. and Hoffmann, William A. and Vasconcelos, Heraldo L. and Pilon, Natashi A. and Rossatto, Davi R. and Durigan, Giselda}, year={2017}, month={Aug} }
 @article{giroldo_scariot_hoffmann_2017, title={Trait shifts associated with the subshrub life-history strategy in a tropical savanna}, volume={185}, ISSN={["1432-1939"]}, DOI={10.1007/s00442-017-3930-4}, abstractNote={Over the past 10 million years, tropical savanna environments have selected for small growth forms within woody plant lineages. The result has been the evolution of subshrubs (geoxyles), presumably as an adaptation to frequent fire. To evaluate the traits associated with the shift from tree to subshrub growth forms, we compared seed biomass, germination, survival, resprouting, biomass allocation, and photosynthesis between congeneric trees and subshrubs, and quantified phylogenetic conservatism. Despite large differences in adult morphology between trees and subshrub species, the differences are modest in seedlings, and most of the variation in traits was explained by genus, indicating considerable phylogenic conservatism. Regardless, tree seedlings invested more heavily in aboveground growth, compared to subshrubs, which is consistent with the adult strategy of savanna trees, which depend on a large resistant-fire stem. Subshrub seedlings also invest in greater non-structural carbohydrate reserves, likely as an adaptation to the high fire frequencies typical of tropical savannas. The modest differences as seedlings suggest that selective pressures during early development may not have contributed substantially to the evolution of the subshrub growth form and that the distinct allocation and life history must arise later in life. This is consistent with the interpretation that the subshrub growth form arose as a life-history strategy in which maturity is reached at a small stem size, allowing them to reproduce despite repeated fire-induced topkill. The convergent evolution of subshrubs within multiple tree lineages reaffirms the importance of fire in the origin and diversification of the flora of mesic savannas.}, number={2}, journal={OECOLOGIA}, author={Giroldo, A. B. and Scariot, A. and Hoffmann, W. A.}, year={2017}, month={Oct}, pages={281–291} }
 @article{just_schafer_hohmann_hoffmann_2017, title={Wood decay and the persistence of resprouting species in pyrophilic ecosystems}, volume={31}, ISSN={["1432-2285"]}, DOI={10.1007/s00468-016-1477-3}, number={1}, journal={TREES-STRUCTURE AND FUNCTION}, author={Just, Michael G. and Schafer, Jennifer L. and Hohmann, Matthew G. and Hoffmann, William A.}, year={2017}, month={Feb}, pages={237–245} }
 @article{geng_liu_ji_hoffmann_grunden_xiang_2016, title={Enhancing Heat Tolerance of the Little Dogwood Cornus canadensis L. f. with Introduction of a Superoxide Reductase Gene from the Hyperthermophilic Archaeon Pyrococcus furiosus}, volume={7}, ISSN={1664-462X}, url={http://dx.doi.org/10.3389/fpls.2016.00026}, DOI={10.3389/fpls.2016.00026}, abstractNote={Production of reactive oxygen species (ROS) can be accelerated under various biotic and abiotic stresses causing lipid peroxidation, protein degradation, enzyme inactivation, and DNA damage. Superoxide reductase (SOR) is a novel antioxidant enzyme from Pyrococcus furiosus and is employed by this anaerobic hyperthermophilic archaeon for efficient detoxification of ROS. In this study, SOR was introduced into a flowering plant Cornus canadensis to enhance its heat tolerance and reduce heat induced damage. A fusion construct of the SOR gene and Green Fluorescent Protein gene (GFP) was introduced into C. canadensis using Agrobacterium-mediated transformation. Heat tolerance of the GFP-SOR expressing transgenic plants was investigated by observing morphological symptoms of heat injury and by examining changes in photosynthesis, malondialdehyde (MDA), and proline levels in the plants. Our results indicate that the expression of the P. furiosus SOR gene in the transgenic plants alleviated lipid peroxidation of cell membranes and photoinhibition of PS II, and decreased the accumulation of proline at 40°C. After a series of exposures to increasing temperatures, the SOR transgenic plants remained healthy and green whereas most of the non-transgenic plants dried up and were unable to recover. While it had previously been reported that expression of SOR in Arabidopsis enhanced heat tolerance, this is the first report of the successful demonstration of improved heat tolerance in a non-model plant resulting from the introduction of P. furiosus SOR. The study demonstrates the potential of SOR for crop improvement and that inherent limitations of plant heat tolerance can be ameliorated with P. furiosus SOR.}, journal={Frontiers in Plant Science}, publisher={Frontiers Media SA}, author={Geng, Xing-Min and Liu, Xiang and Ji, Mikyoung and Hoffmann, William A. and Grunden, Amy and Xiang, Qiu-Yun J.}, year={2016}, month={Jan} }
 @article{pellegrini_franco_hoffmann_2016, title={Shifts in functional traits elevate risk of fire-driven tree dieback in tropical savanna and forest biomes}, volume={22}, ISSN={["1365-2486"]}, DOI={10.1111/gcb.13110}, abstractNote={Abstract}, number={3}, journal={GLOBAL CHANGE BIOLOGY}, author={Pellegrini, Adam F. A. and Franco, Augusto C. and Hoffmann, William A.}, year={2016}, month={Mar}, pages={1235–1243} }
 @article{marchin_broadhead_bostic_dunn_hoffmann_2016, title={Stomatal acclimation to vapour pressure deficit doubles transpiration of small tree seedlings with warming}, volume={39}, ISSN={0140-7791}, url={http://dx.doi.org/10.1111/pce.12790}, DOI={10.1111/pce.12790}, abstractNote={Abstract}, number={10}, journal={Plant, Cell & Environment}, publisher={Wiley}, author={Marchin, Renée M. and Broadhead, Alice A. and Bostic, Laura E. and Dunn, Robert R. and Hoffmann, William A.}, year={2016}, month={Aug}, pages={2221–2234} }
 @article{just_hohmann_hoffmann_2016, title={Where fire stops: vegetation structure and microclimate influence fire spread along an ecotonal gradient}, volume={217}, ISSN={["1573-5052"]}, DOI={10.1007/s11258-015-0545-x}, number={6}, journal={PLANT ECOLOGY}, author={Just, Michael G. and Hohmann, Matthew G. and Hoffmann, William A.}, year={2016}, month={Jun}, pages={631–644} }
 @article{carlson_dole_matthysse_hoffmann_kornegay_2015, title={Bacteria species and solution pH effect postharvest quality of cut Zinnia elegans}, volume={194}, ISSN={["1879-1018"]}, DOI={10.1016/j.scienta.2015.07.044}, abstractNote={Bacterial growth in vase solutions can lead to stem vasculature blockage causing petal and leaf wilt, bent neck, or similar symptoms related to water stress that reduce vase life. In these studies we isolated, identified, and evaluated the effects of several bacteria species on the vase life of cut Zinnia elegans L. ‘Benary’s Giant Wine’. Nine bacterial species were isolated during postharvest testing of cut zinnia stems: Pseudomonas fulva, Serratia ficaria, Rhizobium radiobacter, Chryseobacterium sp., Pantoea ananatis, Bacillus pumilus, Chryseobacterium daejeonense, Brevundimonas sp., and Pseudomonas marginalis and pure cultures of each species were added to the vase solution of cut zinnia stems. Escherichia coli K12, a lab adapted strain, was also included. Cut flowers inoculated with P. fulva and E. coli K12 had significantly greater vase lives of 9.5 and 9.4 d, respectively, compared to P. marginalis, P. ananatis, R. radiobacter, or the nutrient broth control (7.0, 6.9, 6.8, or 7.3 d, respectively). The vase lives of the other bacteria treatments were not statistically different from the deionized (DI) water control (8.6 d). There were no significant differences in water uptake or vase water bacteria concentrations at termination among all treatments. In further studies, sterilized and non-sterilized stems of Zinnia were used to investigate the effects of solution pH and the addition of P. marginalis and E. coli K12 on number of days to drought stress (DTDS), stem hydraulic conductivity, and bacteria concentrations inside and outside the stem. The non-sterilized stems in control solution with E. coli K12 and non-sterilized stems in preservative solution with no bacteria had the most DTDS of 8.0 d. The sterilized stems in the control solution (deionized water) with E. coli K12 and sterilized stems in basic solution with no bacteria had the least DTDS of 5.5 d and 5.8 d, respectively. The concentrations of bacteria inside and outside the stems were lowest for stems in the preservative solutions. Of the stems that were sterilized, partial percent loss of conductivity (PPLC) was significantly lower in the acidic solutions (64%) compared to the preservative (87%) and control (83%). This research shows that for Zinnia the bacteria species that has a primary effect on vase life, not necessarily the concentration of bacteria in the vase solution.}, journal={SCIENTIA HORTICULTURAE}, author={Carlson, Alicain S. and Dole, John M. and Matthysse, Ann G. and Hoffmann, William A. and Kornegay, Julia L.}, year={2015}, month={Oct}, pages={71–78} }
 @article{schafer_breslow_hohmann_hoffmann_2015, title={RELATIVE BARK THICKNESS IS CORRELATED WITH TREE SPECIES DISTRIBUTIONS ALONG A FIRE FREQUENCY GRADIENT}, volume={11}, ISSN={["1933-9747"]}, DOI={10.4996/fireecology.1101074}, abstractNote={Abstract}, number={1}, journal={FIRE ECOLOGY}, author={Schafer, Jennifer L. and Breslow, Bradley P. and Hohmann, Matthew G. and Hoffmann, William A.}, year={2015}, pages={74–87} }
 @article{marchin_salk_hoffmann_dunn_2015, title={Temperature alone does not explain phenological variation of diverse temperate plants under experimental warming}, volume={21}, ISSN={["1365-2486"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84937524423&partnerID=MN8TOARS}, DOI={10.1111/gcb.12919}, abstractNote={Abstract}, number={8}, journal={GLOBAL CHANGE BIOLOGY}, author={Marchin, Renee M. and Salk, Carl F. and Hoffmann, William A. and Dunn, Robert R.}, year={2015}, month={Aug}, pages={3138–3151} }
 @article{marchin_dunn_hoffmann_2014, title={Are winter-active species vulnerable to climate warming? A case study with the wintergreen terrestrial orchid, Tipularia discolor}, volume={176}, ISSN={["1432-1939"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84921938451&partnerID=MN8TOARS}, DOI={10.1007/s00442-014-3074-8}, abstractNote={In the eastern United States, winter temperature has been increasing nearly twice as fast as summer temperature, but studies of warming effects on plants have focused on species that are photosynthetically active in summer. The terrestrial orchid Tipularia discolor is leafless in summer and acquires C primarily in winter. The optimum temperature for photosynthesis in T. discolor is higher than the maximum temperature throughout most of its growing season, and therefore growth can be expected to increase with warming. Contrary to this hypothesis, experimental warming negatively affected reproductive fitness (number of flowering stalks, flowers, fruits) and growth (change in leaf area from 2010 to 2012) in T. discolor. Temperature in June-July was critical for flowering, and mean July temperature greater than 29 °C (i.e., 2.5 °C above ambient) eliminated reproduction. Warming of 1.2 °C delayed flowering by an average of 10 days and fruiting by an average of 5 days. Warming of 4.4 °C reduced relative growth rates by about 60%, which may have been partially caused by the direct effects of temperature on photosynthesis and respiration. Warming indirectly increased vapor pressure deficit (VPD) by 0.2-0.5 kPa, and leaf-to-air VPD over 1.3 kPa restricted stomatal conductance of T. discolor to 10-40% of maximum conductance. These results highlight the need to account for changes in VPD when estimating temperature responses of plant species under future warming scenarios. Increasing temperature in the future will likely be an important limiting factor to the distribution of T. discolor, especially along the southern edge of its range.}, number={4}, journal={OECOLOGIA}, author={Marchin, Renee M. and Dunn, Robert R. and Hoffmann, William A.}, year={2014}, month={Dec}, pages={1161–1172} }
 @article{pellegrini_hoffmann_franco_2014, title={Carbon accumulation and nitrogen pool recovery during transitions from savanna to forest in central Brazil}, volume={95}, ISSN={["1939-9170"]}, DOI={10.1890/13-0290.1}, abstractNote={The expansion of tropical forest into savanna may potentially be a large carbon sink, but little is known about the patterns of carbon sequestration during transitional forest formation. Moreover, it is unclear how nutrient limitation, due to extended exposure to fire‐driven nutrient losses, may constrain carbon accumulation. Here, we sampled plots that spanned a woody biomass gradient from savanna to transitional forest in response to differential fire protection in central Brazil. These plots were used to investigate how the process of transitional forest formation affects the size and distribution of carbon (C) and nitrogen (N) pools. This was paired with a detailed analysis of the nitrogen cycle to explore possible connections between carbon accumulation and nitrogen limitation. An analysis of carbon pools in the vegetation, upper soil, and litter shows that the transition from savanna to transitional forest can result in a fourfold increase in total carbon (from 43 to 179 Mg C/ha) with a doubling of carbon stocks in the litter and soil layers. Total nitrogen in the litter and soil layers increased with forest development in both the bulk (+68%) and plant‐available (+150%) pools, with the most pronounced changes occurring in the upper layers. However, the analyses of nitrate concentrations, nitrate : ammonium ratios, plant stoichiometry of carbon and nitrogen, and soil and foliar nitrogen isotope ratios suggest that a conservative nitrogen cycle persists throughout forest development, indicating that nitrogen remains in low supply relative to demand. Furthermore, the lack of variation in underlying soil type (>20 cm depth) suggests that the biogeochemical trends across the gradient are driven by vegetation. Our results provide evidence for high carbon sequestration potential with forest encroachment on savanna, but nitrogen limitation may play a large and persistent role in governing carbon sequestration in savannas or other equally fire‐disturbed tropical landscapes. In turn, the link between forest development and nitrogen pool recovery creates a framework for evaluating potential positive feedbacks on savanna–forest boundaries.}, number={2}, journal={ECOLOGY}, author={Pellegrini, Adam F. A. and Hoffmann, William A. and Franco, Augusto C.}, year={2014}, month={Feb}, pages={342–352} }
 @article{wall_douglas_hoffmann_wentworth_gray_xiang_knaus_hohmann_g._2014, title={Evidence of population bottleneck in Astragalus michauxii (Fabaceae), a narrow endemic of the southeastern United States}, volume={15}, ISSN={1566-0621, 1572-9737}, url={http://link.springer.com/10.1007/s10592-013-0527-2}, DOI={10.1007/s10592-013-0527-2}, number={1}, journal={Conservation Genetics}, publisher={Springer Science and Business Media LLC}, author={Wall, W. A. and Douglas, N. A. and Hoffmann, W. A. and Wentworth, T. R. and Gray, J. B. and Xiang, Q. Y. J. and Knaus, B. K. and Hohmann and G., M.}, year={2014}, month={Feb}, pages={153–164} }
 @article{lehmann_anderson_sankaran_higgins_archibald_hoffmann_hanan_williams_fensham_felfili_et al._2014, title={Savanna vegetation-fire-climate relationships differ among continents}, volume={343}, number={6170}, journal={Science}, author={Lehmann, C. E. R. and Anderson, T. M. and Sankaran, M. and Higgins, S. I. and Archibald, S. and Hoffmann, W. A. and Hanan, N. P. and Williams, R. J. and Fensham, R. J. and Felfili, J. and et al.}, year={2014}, pages={548–552} }
 @article{schafer_breslow_hollingsworth_hohmann_hoffmann_2014, title={Size-dependent enhancement of water relations during post-fire resprouting}, volume={34}, ISSN={["1758-4469"]}, DOI={10.1093/treephys/tpu015}, abstractNote={In resprouting species, fire-induced topkill causes a reduction in height and leaf area without a comparable reduction in the size of the root system, which should lead to an increase in the efficiency of water transport after fire. However, large plants undergo a greater relative reduction in size, compared with small plants, so we hypothesized that this enhancement in hydraulic efficiency would be greatest among large growth forms. In the ecotone between long-leaf pine (Pinus palustris Mill.) savannas and wetlands, we measured stomatal conductance (gs), mid-day leaf water potential (Ψleaf), leaf-specific whole-plant hydraulic conductance (KL.p), leaf area and height of 10 species covering a range of growth forms in burned and unburned sites. As predicted, KL.p was higher in post-fire resprouts than in unburned plants, and the post-fire increase in KL.p was positively related to plant size. Specifically, large-statured species tended to undergo the greatest relative reductions in leaf area and height, and correspondingly experienced the greatest increases in KL.p. The post-fire increase in KL.p was smaller than expected, however, due to a decrease in absolute root hydraulic conductance (i.e., not scaled to leaf area). The higher KL.p in burned sites was manifested as an increase in gs rather than an increase in Ψleaf. Post-fire increases in gs should promote high rates of photosynthesis for recovery of carbohydrate reserves and aboveground biomass, which is particularly important for large-statured species that require more time to recover their pre-fire size.}, number={4}, journal={TREE PHYSIOLOGY}, author={Schafer, Jennifer L. and Breslow, Bradley P. and Hollingsworth, Stephanie N. and Hohmann, Matthew G. and Hoffmann, William A.}, year={2014}, month={Apr}, pages={404–414} }
 @article{parr_lehmann_bond_hoffmann_andersen_2014, title={Tropical grassy biomes: misunderstood, neglected, and under threat}, volume={29}, ISSN={["1872-8383"]}, DOI={10.1016/j.tree.2014.02.004}, abstractNote={Tropical grassy biomes (TGBs) are globally extensive, provide critical ecosystem services, and influence the earth-atmosphere system. Yet, globally applied biome definitions ignore vegetation characteristics that are critical to their functioning and evolutionary history. Hence, TGB identification is inconsistent and misinterprets the ecological processes governing vegetation structure, with cascading negative consequences for biodiversity. Here, we discuss threats linked to the definition of TGB, the Clean Development Mechanism (CDM) and Reducing Emissions from Deforestation and Forest Degradation schemes (REDD+), and enhanced atmospheric CO2, which may facilitate future state shifts. TGB degradation is insidious and less visible than in forested biomes. With human reliance on TGBs and their propensity for woody change, ecology and evolutionary history are fundamental to not only the identification of TGBs, but also their management for future persistence.}, number={4}, journal={TRENDS IN ECOLOGY & EVOLUTION}, author={Parr, Catherine L. and Lehmann, Caroline E. R. and Bond, William J. and Hoffmann, William A. and Andersen, Alan N.}, year={2014}, month={Apr}, pages={205–213} }
 @article{silva_hoffmann_rossatto_haridasan_franco_horwath_2013, title={Can savannas become forests? A coupled analysis of nutrient stocks and fire thresholds in central Brazil}, volume={373}, ISSN={["1573-5036"]}, DOI={10.1007/s11104-013-1822-x}, number={1-2}, journal={PLANT AND SOIL}, author={Silva, Lucas C. R. and Hoffmann, William A. and Rossatto, Davi R. and Haridasan, Mundayatan and Franco, Augusto C. and Horwath, William R.}, year={2013}, month={Dec}, pages={829–842} }
 @article{schafer_breslow_just_hohmann_hollingsworth_swatling-holcomb_hoffmann_2013, title={Current and Historical Variation in Wiregrass (Aristida stricta) Abundance and Distribution Is Not Detectable from Soil delta C-13 Measurements in Longleaf Pine (Pinus palustris) Savannas}, volume={78}, ISSN={["1938-4386"]}, DOI={10.2179/12-021}, abstractNote={ABSTRACT Plant species distributions and transitions between vegetation types are determined by numerous factors, including disturbances such as fire. Documentation of past changes in the distribution and structure of fire-dependent ecosystems is necessary to assess the success of land management in maintaining historic vegetation types. In our study system—longleaf pine (Pinus palustris)-wiregrass (Aristida stricta) savannas and embedded wetlands (i.e., pocosins)—wiregrass is dependent on frequent burning. We used soil carbon (C) isotopes to test for past changes in the abundance of wiregrass and for shifts in the ecotone between savanna and pocosin in four sites at Fort Bragg, North Carolina. Wiregrass has a C4 photosynthetic pathway and therefore produces organic matter enriched in 13C compared to other dominant species in the community that have a C3 photosynthetic pathway. At each of the four sites, we measured profiles of soil δ13C to a depth of 1 m at four locations along the vegetation gradient from upland savannas to lowland pocosins. Surface soil δ13C, which reflects C inputs from current vegetation, did not differ along the gradient despite a decrease in wiregrass cover from savanna, where it is abundant, to pocosin, where it is absent. Enrichment of soil δ13C with depth was indistinguishable from effects of decomposition, indicating that past variation in the abundance and distribution of wiregrass was not detectable in our study sites. Our results suggest that wiregrass currently does not produce, and historically has not produced, enough biomass to influence soil δ13C values along the savanna-pocosin gradient.}, number={1}, journal={CASTANEA}, author={Schafer, Jennifer L. and Breslow, Bradley P. and Just, Michael G. and Hohmann, Matthew G. and Hollingsworth, Stephanie N. and Swatling-Holcomb, Samantha L. and Hoffmann, William A.}, year={2013}, month={Mar}, pages={28–36} }
 @article{rossatto_hoffmann_ramos silva_haridasan_sternberg_franco_2013, title={Seasonal variation in leaf traits between congeneric savanna and forest trees in Central Brazil: implications for forest expansion into savanna}, volume={27}, ISSN={["1432-2285"]}, DOI={10.1007/s00468-013-0864-2}, number={4}, journal={TREES-STRUCTURE AND FUNCTION}, author={Rossatto, Davi Rodrigo and Hoffmann, William Arthur and Ramos Silva, Lucas de Carvalho and Haridasan, Mundayatan and Sternberg, Leonel S. L. and Franco, Augusto Cesar}, year={2013}, month={Aug}, pages={1139–1150} }
 @article{grady_hoffmann_2012, title={Caught in a fire trap: Recurring fire creates stable size equilibria in woody resprouters}, volume={93}, ISSN={["1939-9170"]}, DOI={10.1890/12-0354.1}, abstractNote={Globally, fire maintains many mesic habitats in an open canopy state by killing woody plants while reducing the size of those able to resprout. Where fire is frequent, tree saplings are often suppressed by a “fire trap” of repeated topkill (death of aerial biomoass) and resprouting, preventing them from reaching adult size. The ability to tolerate repeated topkill is an essential life‐history trait that allows a sapling to persist until it experiences a long fire‐free interval, during which it can escape the fire trap. We hypothesized that persistence in the fire trap results from a curvilinear relationship between pre‐burn size and resprout size, which causes a plant to approach an equilibrial size in which post‐fire biomass recovery is equal to fire‐induced biomass loss. We also predicted that the equilibrial stem size is positively related to resource availability. To test these hypotheses, we collected data on pre‐burn and resprout size of five woody plant species at wetland ecotones in longleaf pine savanna subjected to frequent burning. As expected, all species exhibited similar curvilinear relationships between pre‐burn size and resprout size. The calculated equilibrial sizes were strong predictors of mean plant size across species and growing conditions, supporting the persistence equilibrium model. An alternative approach using matrix models yielded similar results. Resprouting was less vigorous in dry sites than at wet sites, resulting in smaller equilibrial stem sizes in drier sites; extrapolating these results provides an explanation for the absence of these species in xeric uplands. This new framework offers a straightforward approach to guide data collection for experimental, comparative, and modeling studies of plant persistence and community dynamics in frequently burned habitats.}, number={9}, journal={ECOLOGY}, author={Grady, John M. and Hoffmann, William A.}, year={2012}, month={Sep}, pages={2052–2060} }
 @article{wall_hoffmann_wentworth_gray_hohmann_2012, title={Demographic effects of fire on two endemic plant species in the longleaf pine-wiregrass ecosystem}, volume={213}, ISSN={["1385-0237"]}, DOI={10.1007/s11258-012-0068-7}, number={7}, journal={PLANT ECOLOGY}, author={Wall, Wade A. and Hoffmann, William A. and Wentworth, Thomas R. and Gray, Janet B. and Hohmann, Matthew G.}, year={2012}, month={Jul}, pages={1093–1104} }
 @misc{hoffmann_geiger_gotsch_rossatto_silva_lau_haridasan_franco_2012, title={Ecological thresholds at the savanna-forest boundary: how plant traits, resources and fire govern the distribution of tropical biomes}, volume={15}, ISSN={["1461-0248"]}, DOI={10.1111/j.1461-0248.2012.01789.x}, abstractNote={Abstract}, number={7}, journal={ECOLOGY LETTERS}, author={Hoffmann, William A. and Geiger, Erika L. and Gotsch, Sybil G. and Rossatto, Davi R. and Silva, Lucas C. R. and Lau, On Lee and Haridasan, M. and Franco, Augusto C.}, year={2012}, month={Jul}, pages={759–768} }
 @article{hoffmann_jaconis_mckinley_geiger_gotsch_franco_2012, title={Fuels or microclimate? Understanding the drivers of fire feedbacks at savanna-forest boundaries}, volume={37}, ISSN={["1442-9985"]}, DOI={10.1111/j.1442-9993.2011.02324.x}, abstractNote={Abstract}, number={6}, journal={AUSTRAL ECOLOGY}, author={Hoffmann, William A. and Jaconis, Susany. and Mckinley, Kristen L. and Geiger, Erika L. and Gotsch, Sybil G. and Franco, Augusto C.}, year={2012}, month={Sep}, pages={634–643} }
 @article{lehmann_archibald_hoffmann_bond_2011, title={Deciphering the distribution of the savanna biome}, volume={191}, ISSN={["1469-8137"]}, DOI={10.1111/j.1469-8137.2011.03689.x}, abstractNote={• We aimed to identify the limits of savanna across Africa, Australia and South America. We based our investigation on the rich history of hypotheses previously examined: that the limits of savanna are variously determined by rainfall, rainfall seasonality, soil fertility and disturbance. • We categorized vegetation on all continents as 'savanna' (open habitats with a C(4) grass layer) or 'not-savanna' (closed habitats with no C(4) grass layer) and used a combination of statistical approaches to examine how the presence of savanna varied as a function of five environmental correlates. • The presence of savanna is constrained by effective rainfall and rainfall seasonality. Soil fertility is regionally important, although the direction of its effect changes relative to rainfall. We identified three continental divergences in the limits of savanna that could not be explained by environment. • Climate and soils do not have a deterministic effect on the distribution of savanna. Over the range of savanna, some proportion of the land is always 'not-savanna'. We reconciled previous contradictory views of savanna limits by developing a new conceptual framework for understanding these limits by categorizing environmental factors into whether they had a positive or negative effect on woody growth and the frequency of disturbance.}, number={1}, journal={NEW PHYTOLOGIST}, author={Lehmann, Caroline E. R. and Archibald, Sally A. and Hoffmann, William A. and Bond, William J.}, year={2011}, pages={197–209} }
 @article{geiger_gotsch_damasco_haridasan_franco_hoffmann_2011, title={Distinct roles of savanna and forest tree species in regeneration under fire suppression in a Brazilian savanna}, volume={22}, ISSN={["1654-1103"]}, DOI={10.1111/j.1654-1103.2011.01252.x}, abstractNote={Questions: Has fire suppression relaxed barriers to the exchange of species between savanna and forest? Do all species or a subset of species participate in this exchange? Would current vegetation structure persist if fire suppression were to cease? Location: A gallery forest edge in the Cerrado region of central Brazil that burned only once in the past 35 years. Methods: Density of tree seedlings, saplings and adults, leaf area index (LAI), tree basal area and diameter were surveyed in 12, 10m � 70m transects centred on and perpendicular to the forest‐savanna boundary. Community composition was assessed using non-metric multi-dimensional scaling (NMDS). Results: Basal area and LAI declined substantially from forest to savanna, with an associated shift in species composition. Savanna tree species were nearly absent in the forest, but accounted for the majority of stems in the savanna. In contrast, forest species comprised 14% of adults and more than one-third of juveniles in the savanna. Despite the high diversity of trees (85 species) in the forest, five species play a particularly large role in this initial phase of forest expansion. Reintroduction of fire, however, would result in widespread topkill of juveniles and the majority of adult forest trees, thereby interrupting the succession towards forest. Conclusions: After 35 years during which the site burned only once, the savanna still remains dominated by savanna species. Nevertheless, the dominance of forest juveniles in border and savanna tree communities suggests that with a continued policy of fire suppression, the forest will continue to expand.}, number={2}, journal={JOURNAL OF VEGETATION SCIENCE}, author={Geiger, Erika L. and Gotsch, Sybil G. and Damasco, Gabriel and Haridasan, M. and Franco, Augusto C. and Hoffmann, William A.}, year={2011}, month={Apr}, pages={312–321} }
 @article{hoffmann_marchin_abit_lau_2011, title={Hydraulic failure and tree dieback are associated with high wood density in a temperate forest under extreme drought}, volume={17}, ISSN={["1365-2486"]}, DOI={10.1111/j.1365-2486.2011.02401.x}, abstractNote={Catastrophic hydraulic failure will likely be an important mechanism contributing to large‐scale tree dieback caused by increased frequency and intensity of droughts under global climate change. To compare the susceptibility of 22 temperate deciduous tree and shrub species to hydraulic failure during a record drought in the southeastern USA, we quantified leaf desiccation, native embolism, wood density, stomatal conductance and predawn and midday leaf water potential at four sites with varying drought intensities. At the two driest sites, there was widespread leaf wilting and desiccation, and most species exhibited predawn leaf water potentials of ≤3 MPa and >60% loss of xylem conductivity in branches. Although species with high wood density were more resistant to cavitation, they had higher levels of native embolism and greater canopy dieback than species with low wood density. This unexpected result can be explained by the failure of species with dense wood to avert a decline in water potential to dangerous levels during the drought. Leaf water potential was negatively correlated with wood density, and the relationship was strongest under conditions of severe water deficit. Species with low wood density avoided catastrophic embolism by relying on an avoidance strategy that involves partial drought deciduousness, higher sensitivity of stomata to leaf water potential and perhaps greater rooting depth. These species therefore maintained water potential at levels that ensured a greater margin of safety against embolism. These differences among species may mediate rapid shifts in species composition of temperate forests if droughts intensify due to climate change.}, number={8}, journal={GLOBAL CHANGE BIOLOGY}, author={Hoffmann, William A. and Marchin, Renee M. and Abit, Pamela and Lau, On Lee}, year={2011}, month={Aug}, pages={2731–2742} }
 @article{wall_wentworth_shelingoski_stucky_leblond_hoffmann_2011, title={Lost and Found: Remnants of the Big Savannah and Their Relationship to Wet Savannas in North Carolina}, volume={76}, ISSN={["1938-4386"]}, DOI={10.2179/10-046.1}, abstractNote={Abstract Conversion to agriculture and plantations, development, and fire suppression have reduced the extent of savannas in the southeastern United States, and there is a need to catalog and classify the remaining savannas for both restoration and resource management purposes. The Big Savannah was a wet savanna in North Carolina that was destroyed in the 1950s, and subsequent vegetation classifications have generally not accommodated well the unique natural plant community of the Big Savannah. Vegetation reminiscent of that described for the Big Savannah was discovered north of the original site and designated as Wells Savannah. To evaluate the uniqueness of the savanna vegetation at Wells Savannah, we compiled a data set from permanent quadrats with information on vegetation and environmental variables from other Outer Coastal Plain savannas to compare with similar data from the natural community at Wells Savannah. We also inventoried an additional 26 quadrats on a tract adjacent to Wells Savannah that had experienced fire suppression. Results from multivariate analyses demonstrated clear differences between the Wells Savannah quadrats and other regional wet savanna quadrats based on both vegetation and soils. A number of species and several soil characteristics (higher clay percentage, and higher available iron and boron) distinguish Wells Savannah from other wet savannas. Although the fire suppressed quadrats near Wells Savannah had lower species richness, typical savanna species such as Ctenium aromaticum and Calamovilfa brevipilis were still present. Further exploration of fire-suppressed tracts in the area may yield more wet savanna inclusions similar to the former Big Savannah.}, number={4}, journal={CASTANEA}, author={Wall, W. A. and Wentworth, T. R. and Shelingoski, S. and Stucky, J. M. and LeBlond, R. J. and Hoffmann, W. A.}, year={2011}, month={Dec}, pages={348–363} }
 @article{douglas_wall_xiang_hoffmann_wentworth_gray_hohmann_2011, title={Recent vicariance and the origin of the rare, edaphically specialized Sandhills lily, Lilium pyrophilum (Liliaceae): evidence from phylogenetic and coalescent analyses}, volume={20}, ISSN={["0962-1083"]}, DOI={10.1111/j.1365-294x.2011.05151.x}, abstractNote={Abstract}, number={14}, journal={MOLECULAR ECOLOGY}, author={Douglas, Norman A. and Wall, Wade A. and Xiang, Qiu-Yun and Hoffmann, William A. and Wentworth, Thomas R. and Gray, Janet B. and Hohmann, Matthew G.}, year={2011}, month={Jul}, pages={2901–2915} }
 @article{ratnam_bond_fensham_hoffmann_archibald_lehmann_anderson_higgins_sankaran_2011, title={When is a 'forest' a savanna, and why does it matter?}, volume={20}, ISSN={["1466-8238"]}, DOI={10.1111/j.1466-8238.2010.00634.x}, abstractNote={Savannas are defined based on vegetation structure, the central concept being a discontinuous tree cover in a continuous grass understorey. However, at the high-rainfall end of the tropical savanna biome, where heavily wooded mesic savannas begin to structurally resemble forests, or where tropical forests are degraded such that they open out to structurally resemble savannas, vegetation structure alone may be inadequate to distinguish mesic savanna from forest. Additional knowledge of the functional differences between these ecosystems which contrast sharply in their evolutionary and ecological history is required. Specifically, we suggest that tropical mesic savannas are predominantly mixed tree–C4 grass systems defined by fire tolerance and shade intolerance of their species, while forests, from which C4 grasses are largely absent, have species that are mostly fire intolerant and shade tolerant. Using this framework, we identify a suite of morphological, physiological and life-history traits that are likely to differ between tropical mesic savanna and forest species. We suggest that these traits can be used to distinguish between these ecosystems and thereby aid their appropriate management and conservation. We also suggest that many areas in South Asia classified as tropical dry forests, but characterized by fire-resistant tree species in a C4 grass-dominated understorey, would be better classified as mesic savannas requiring fire and light to maintain the unique mix of species that characterize them.}, number={5}, journal={GLOBAL ECOLOGY AND BIOGEOGRAPHY}, author={Ratnam, Jayashree and Bond, William J. and Fensham, Rod J. and Hoffmann, William A. and Archibald, Sally and Lehmann, Caroline E. R. and Anderson, Michael T. and Higgins, Steven I. and Sankaran, Mahesh}, year={2011}, month={Sep}, pages={653–660} }
 @article{gotsch_geiger_franco_goldstein_meinzer_hoffmann_2010, title={Allocation to leaf area and sapwood area affects water relations of co-occurring savanna and forest trees}, volume={163}, ISSN={["1432-1939"]}, DOI={10.1007/s00442-009-1543-2}, abstractNote={Water availability is a principal factor limiting the distribution of closed-canopy forest in the seasonal tropics, suggesting that forest tree species may not be well adapted to cope with seasonal drought. We studied 11 congeneric species pairs, each containing one forest and one savanna species, to test the hypothesis that forest trees have a lower capacity to maintain seasonal homeostasis in water relations relative to savanna species. To quantify this, we measured sap flow, leaf water potential (Psi(L)), stomatal conductance (g (s)), wood density, and Huber value (sapwood area:leaf area) of the 22 study species. We found significant differences in the water relations of these two species types. Leaf area specific hydraulic conductance of the soil/root/leaf pathway (G (t)) was greater for savanna species than forest species. The lower G (t) of forest trees resulted in significantly lower Psi(L) and g (s) in the late dry season relative to savanna trees. The differences in G (t) can be explained by differences in biomass allocation of savanna and forest trees. Savanna species had higher Huber values relative to forest species, conferring greater transport capacity on a leaf area basis. Forest trees have a lower capacity to maintain homeostasis in Psi(L) due to greater allocation to leaf area relative to savanna species. Despite significant differences in water relations, relationships between traits such as wood density and minimum Psi(L) were indistinguishable for the two species groups, indicating that forest and savanna share a common axis of water-use strategies involving multiple traits.}, number={2}, journal={OECOLOGIA}, author={Gotsch, Sybil G. and Geiger, Erika L. and Franco, Augusto C. and Goldstein, Guillermo and Meinzer, Frederick C. and Hoffmann, William A.}, year={2010}, month={Jun}, pages={291–301} }
 @article{marchin_zeng_hoffmann_2010, title={Drought-deciduous behavior reduces nutrient losses from temperate deciduous trees under severe drought}, volume={163}, ISSN={["1432-1939"]}, DOI={10.1007/s00442-010-1614-4}, abstractNote={Nutrient resorption from senescing leaves is an important mechanism of nutrient conservation in temperate deciduous forests. Resorption, however, may be curtailed by climatic events that cause rapid leaf death, such as severe drought, which has been projected to double by the year 2100 in the eastern United States. During a record drought in the southeastern US, we studied 18 common temperate winter-deciduous trees and shrubs to understand how extreme drought affects nutrient resorption of the macronutrients N, P, K, and Ca. Four species exhibited drought-induced leaf senescence and maintained higher leaf water potentials than the remaining 14 species (here called drought-evergreen species). This strategy prevented extensive leaf desiccation during the drought and successfully averted large nutrient losses caused by leaf desiccation. These four drought-deciduous species were also able to resorb N, P, and K from drought-senesced leaves, whereas drought-evergreen species did not resorb any nutrients from leaves lost to desiccation during the drought. For Oxydendrum arboreum, the species most severely affected by the drought, our results indicate that trees lost 50% more N and P due to desiccation than would have been lost from fall senescence alone. For all drought-deciduous species, resorption of N and P in fall-senesced leaves was highly proficient, whereas resorption was incomplete for drought-evergreen species. The lower seasonal nutrient losses of drought-deciduous species may give them a competitive advantage over drought-evergreen species in the years following the drought, thereby impacting species composition in temperate deciduous forests in the future.}, number={4}, journal={OECOLOGIA}, author={Marchin, Renee and Zeng, Hainian and Hoffmann, William}, year={2010}, month={Aug}, pages={845–854} }
 @article{wall_hilton_wentworth_gray_hohmann_hoffmann_2010, title={Effects of light and temperature on germination of Pyxidanthera brevifolia Wells (Diapensiaceae)}, volume={137}, ISSN={["1940-0616"]}, DOI={10.3159/10-ra-023.1}, abstractNote={Abstract Pyxidanthera brevifolia is an evergreen semi-woody cushion plant endemic to the Sandhills of North and South Carolina, with the majority of populations occurring on Fort Bragg Military Reservation in North Carolina. Currently the species is listed as Endangered in North Carolina and is designated as a Species at Risk (SAR) by the US Department of Defense. Previous studies have suggested that seeds may not be viable because they failed to germinate under controlled conditions. Our objectives in this study were to attempt germination of Pyxidanthera brevifolia seeds, determine the best temperature conditions for germination, and understand more about germination requirements to aid in future restoration efforts. Using seeds that had been stored at room temperature for six months, we performed a germination experiment at the NCSU Phytotron with six treatments, all combinations of three temperature regimes (low (18 °C day / 14 °C night), medium (22/18 °C), and high (26/22 °C)) and two light conditions (light and dark). We monitored the experiment for 13 weeks, recording the number of seeds germinating per dish and the number of days to germination for seeds in each treatment. We found that Pxyidanthera brevifolia produces germinable seeds and that there are significant effects of light and temperature on germination. Highest germination occurred under low temperature and high light conditions (78%); the combination of high temperature and no light produced the lowest germination (6%). Seeds exposed to light germinated significantly earlier at the coolest temperature, compared to medium and high temperatures. These results indicate that it is possible to germinate seeds of this rare plant and suggest that germination of Pyxidanthera brevifolia likely occurs in late fall and is dependent on adequate light availability.}, number={4}, journal={JOURNAL OF THE TORREY BOTANICAL SOCIETY}, author={Wall, Wade A. and Hilton, Jacob L. and Wentworth, Thomas R. and Gray, Janet B. and Hohmann, Matthew G. and Hoffmann, William A.}, year={2010}, pages={348–354} }
 @article{scholz_bucci_hoffmann_meinzer_goldstein_2010, title={Hydraulic lift in a Neotropical savanna: Experimental manipulation and model simulations}, volume={150}, ISSN={["1873-2240"]}, DOI={10.1016/j.agrformet.2010.02.001}, abstractNote={The objective of this study was to assess the magnitude of hydraulic lift in Brazilian savannas (Cerrado) and to test the hypothesis that hydraulic lift by herbaceous plants contributes substantially to slowing the decline of water potential and water storage in the upper soil layers during the dry season. To this effect, field observations of soil water content and water potentials, an experimental manipulation, and model simulations, were used. Savannas of central Brazil exhibit consistent changes in tree density along shallow topographic gradients, from open savannas with relatively few trees in the lower portions of the topographic gradients to woodlands with a relatively high density of trees in the upper portions of the gradient. Herbaceous plant abundance follows the opposite trend. The diel recovery of soil water storage was higher in sites with more abundant herbaceous vegetation. Experimental removal of the above ground portion of herbaceous plants in a site with low tree density, during the dry season, substantially enhanced diel recovery of water potentials in the upper soil layers, consistent with the release of hydraulically lifted water from their shallow roots. In a site with high tree density, the release of hydraulically lifted water by woody plants contributed only 2% to the partial daily recovery of soil water storage, whereas the herbaceous layer contributed the remaining 98%. Non saturated water flow in the same savanna type contributed 8% to the partial daily recovery of soil water potential at the beginning of the dry season, decreasing to near 0% after 20 days of drought. During a 70-day rainless period the soil water potential dropped to −2.0 MPa near the soil surface. The simulation model predicted that without hydraulic lift, water potential in the upper soil layers in relatively dense savannas would have dropped to −3.8 MPa. The maximum contribution of hydraulic lift to the upper 100 cm of soil was 0.7 mm day−1 near the middle of the dry season. During the peak of the dry season, hydraulic lift can replace 23% of the ecosystem evapotranspiration in a site with high tree density and consequently greatly influences the water economy and other ecosystem processes in the Cerrado.}, number={4}, journal={AGRICULTURAL AND FOREST METEOROLOGY}, author={Scholz, Fabian G. and Bucci, Sandra J. and Hoffmann, William A. and Meinzer, Frederick C. and Goldstein, Guillermo}, year={2010}, month={Apr}, pages={629–639} }
 @article{silva_haridasan_sternberg_franco_hoffmann_2010, title={Not all forests are expanding over central Brazilian savannas}, volume={333}, ISSN={["1573-5036"]}, DOI={10.1007/s11104-010-0358-6}, number={1-2}, journal={PLANT AND SOIL}, author={Silva, Lucas C. R. and Haridasan, Mundayatan and Sternberg, Leonel S. L. and Franco, Augusto C. and Hoffmann, William A.}, year={2010}, month={Aug}, pages={431–442} }
 @article{marchin_bhandari_wall_hohmann_gray_hoffmann_2009, title={Are rare species less shade tolerant than common species in fire-prone environments? A test with seven Amorpha (Fabaceae) species}, volume={205}, ISSN={["1573-5052"]}, DOI={10.1007/s11258-009-9614-3}, number={2}, journal={PLANT ECOLOGY}, author={Marchin, Renee M. and Bhandari, Rohit K. and Wall, Wade A. and Hohmann, Matthew G. and Gray, Janet B. and Hoffmann, William A.}, year={2009}, month={Dec}, pages={249–260} }
 @article{rossatto_hoffmann_franco_2009, title={Differences in growth patterns between co-occurring forest and savanna trees affect the forest-savanna boundary}, volume={23}, ISSN={["1365-2435"]}, DOI={10.1111/j.1365-2435.2009.01568.x}, abstractNote={Summary}, number={4}, journal={FUNCTIONAL ECOLOGY}, author={Rossatto, Davi Rodrigo and Hoffmann, William Arthur and Franco, Augusto Cesar}, year={2009}, month={Aug}, pages={689–698} }
 @article{giambelluca_scholz_bucci_meinzer_goldstein_hoffmann_franco_buchert_2009, title={Evapotranspiration and energy balance of Brazilian savannas with contrasting tree density}, volume={149}, ISSN={["1873-2240"]}, DOI={10.1016/j.agrformet.2009.03.006}, abstractNote={Tropical savannas including those of central Brazil (Cerrado) serve as an important regulator of energy and mass exchange with the atmosphere. However, the effects of interactions between grasses and deeply rooted trees and shrubs on energy and water fluxes of savannas are not well understood. In this study, eddy-covariance measurements were used to observe and compare the energy balance and evapotranspiration at two adjacent Cerrado sites differing in woody plant density. Latent energy flux (LE) and evapotranspiration (ET) for Cerrado Denso (8–10-m trees with a cover density of approximately 50% and an understory of grass and shrubs) were generally similar to or greater than for Campo Cerrado (widely scattered 3–4-m trees with a cover density of about 5% and an understory of grasses, shrubs, and sedges), with the greatest difference between the sites occurring during the dry season. Mean annual ET was 823 and 689 mm yr−1, and LE averaged 63.9 ± 11.7 and 53.5 ± 14.3 W m−2 at Cerrado Denso and Campo Cerrado, respectively. At both sites, ET reached a minimum at near the end of the dry season, indicating that reduced water availability constrained dry season ET at both sites. The Bowen ratio at both sites increased as soil moisture decreased, but with a stronger dependency at the Campo Cerrado site, presumably due to the dominance of shallow-rooted plants and the larger contribution of soil evaporation there. Energy partitioning, as indicated by the Bowen ratio, was also strongly influenced by variations in leaf area index (LAI). The strong similarity in the Bowen ratio–LAI relationship for the two sites suggests that LAI can be used to explain much of the observed temporal and spatial ET variability across seasons and variations in woody plant density in the Cerrado.}, number={8}, journal={AGRICULTURAL AND FOREST METEOROLOGY}, author={Giambelluca, Thomas W. and Scholz, Fabian G. and Bucci, Sandra J. and Meinzer, Frederick C. and Goldstein, Guillermo and Hoffmann, William A. and Franco, Augusto C. and Buchert, Martin P.}, year={2009}, month={Aug}, pages={1365–1376} }
 @article{zhang_meinzer_hao_scholz_bucci_takahashi_villalobos-vega_giraldo_cao_hoffmann_et al._2009, title={Size-dependent mortality in a Neotropical savanna tree: the role of height-related adjustments in hydraulic architecture and carbon allocation}, volume={32}, ISSN={["1365-3040"]}, DOI={10.1111/j.1365-3040.2009.02012.x}, abstractNote={ABSTRACT}, number={10}, journal={PLANT CELL AND ENVIRONMENT}, author={Zhang, Yong-Jiang and Meinzer, Frederick C. and Hao, Guang-You and Scholz, Fabian G. and Bucci, Sandra J. and Takahashi, Frederico S. C. and Villalobos-Vega, Randol and Giraldo, Juan P. and Cao, Kun-Fang and Hoffmann, William A. and et al.}, year={2009}, month={Oct}, pages={1456–1466} }
 @article{rossatto_hoffmann_franco_2009, title={Stomatal traits of cerrado and gallery forest congeneric pairs growing in a transitional region in Central Brazil}, volume={23}, ISSN={["1677-941X"]}, DOI={10.1590/S0102-33062009000200021}, abstractNote={Em áreas protegidas do fogo, espécies arbóreas predominantemente florestais conseguem se estabelecer no cerrado e crescer lado a lado com espécies do mesmo gênero que são características destas formações savânicas. Devido às condições ambientais diferenciadas encontradas nas formações de cerrado e de mata, estas espécies congenéricas podem se comportar como grupos funcionais distintos. Neste trabalho foi realizado um estudo comparativo da anatomia dos estômatos e da condutância estomática e taxas de transpiração foliar em 10 pares de espécies congenéricas do cerrado e de mata de galeria e das relações entre as características estomáticas selecionadas e a condutância estomática, já que a morfologia dos estômatos e sua densidade afetam a área para difusão dos gases e sua trajetória através dos poros estomáticos. Cada par foi de uma família diferente. Para a maioria das espécies, a área do poro estomático foi o fator de maior influencia no processo de trocas gasosas, pela sua correlação com a condutância estomática, enquanto a densidade estomática mostrou uma correlação negativa significativa tanto com o comprimento da célula-guarda quanto com a área do poro estomático. As espécies do cerrado apresentaram valores significativamente maiores do comprimento da célula-guarda e do poro estomático. No entanto, para a maioria dos outros parâmetros estomáticos examinados, a maior parte da variação pode ser atribuída a diferenças entre os gêneros, sendo que a variação entre as espécies dos dois ambientes parece ser um produto da historia evolutiva de cada grupo e não reflete uma especialização ao ambiente de cerrado ou de mata de galeria.}, number={2}, journal={ACTA BOTANICA BRASILICA}, author={Rossatto, Davi Rodrigo and Hoffmann, William Arthur and Franco, Augusto Cesar}, year={2009}, pages={499–508} }
 @article{hoffmann_adasme_haridasan_carvalho_geiger_pereira_gotsch_franco_2009, title={Tree topkill, not mortality, governs the dynamics of savanna-forest boundaries under frequent fire in central Brazil}, volume={90}, ISSN={["1939-9170"]}, DOI={10.1890/08-0741.1}, abstractNote={Tropical savanna and forest are recognized to represent alternate stable states, primarily determined by feedbacks with fire. Vegetation–fire dynamics in each of these vegetation types are largely determined by the influence of the vegetation on fire behavior, as well as the effects of fire behavior on tree mortality, topkill (defined here as complete death of the aerial biomass, regardless of whether the plant recovers by resprouting), and rate of growth of resprouts. We studied the effect of fire on three savanna–forest boundaries in central Brazil. Fire intensity was greater in savanna than forest, as inferred by a twofold greater height of stem charring. Despite lower fire intensity, forest tree species exhibited higher rates of topkill, which was best explained by their thinner bark, relative to savanna species. Following topkill, there was no tendency for sprouts of savanna trees to grow faster than those of forest species, contrary to expectations, nor was whole‐plant mortality higher in forest than in savanna. This contrasts with observations of high rates of postburn mortality in many other tropical forests. The low tree mortality in these transitional forests suggests that the dynamic of these natural savanna–forest boundaries is fundamentally different from that of forest boundaries originating from deforestation in the humid tropics. The forests studied here appear to be much more resilient to occasional incursion of fire from the savanna, despite being unable to invade frequently burned savanna. The thin bark of forest species makes them particularly susceptible to the “fire trap,” whereby repeated topkill of small trees prevents recruitment into adult size classes. Rapid growth will be particularly important for forest species to escape the fire trap, so we predict that, where fire is frequent, forests should be restricted to high‐resource sites. Here, Mg2+ and Ca2+ concentrations had particularly strong effects on postburn growth rates, suggesting that these elements may most strongly limit the distribution of forest in these fire‐prone savannas.}, number={5}, journal={ECOLOGY}, author={Hoffmann, William A. and Adasme, Ryan and Haridasan, M. and Carvalho, Marina T. and Geiger, Erika L. and Pereira, Mireia A. B. and Gotsch, Sybil G. and Franco, Augusto C.}, year={2009}, month={May}, pages={1326–1337} }
 @article{bucci_scholz_goldstein_hoffmann_meinzer_franco_giambelluca_miralles-wilhelm_2008, title={Controls on stand transpiration and soil water utilization along a tree density gradient in a Neotropical savanna}, volume={148}, ISSN={["1873-2240"]}, DOI={10.1016/j.agrformet.2007.11.013}, abstractNote={Environmental controls of stand-level tree transpiration (E) and seasonal patterns of soil water utilization were studied in five central Brazilian savanna (Cerrado) sites differing in tree density. Tree density of Cerrado vegetation in the study area consistently changes along topographic gradients from ∼1000 trees ha−1 in open savannas (campo sujo) at the lower end of the topographic gradient to >3000 trees ha−1 in woodlands (cerradão) at the upper end of the gradient. Tree canopy resistance (rC) increased linearly with increasing daily mean air saturation deficit (D) at all sites, but cerradão and cerrado denso sites with higher tree density and higher tree leaf area index (LAI) had lower rC values at all values of D compared to physiognomies with lower tree density, suggesting that rC was less sensitive to changes in D in physiognomies with high tree density and LAI. During the peak of the dry season, mean soil water potential at 0.20 m depth was most negative in the sites with the lowest tree basal area and increased linearly with basal area across sites. In contrast, soil water storage in the 0.10–2.50 m layer decreased exponentially with increasing basal area, consistent with trees in higher density sites utilizing a larger proportion of available soil water at depth during the dry season. Maximum tree transpiration was highest in the cerradão and cerrado denso (∼0.81 mm day−1). Despite higher evaporative demand during the dry season, E was similar between the dry and wet seasons within each study site, which was associated with lower LAI and canopy conductance (gC) during the dry season compared to the wet season. Leaf area index was a good predictor of E and gC. For both dry and wet season data combined, E increased asymptotically with increasing LAI across all physiognomic types, allowing LAI to be used as a predictor of spatial variation of E. The lack of seasonality in E across the Cerrado physiognomies studied could not be explained by individual constraining variables such as D or soil water potential near the surface, but was consistent with the influence of multiple regulatory effects of D and soil water potential on seasonal changes in leaf area and gC.}, number={6-7}, journal={AGRICULTURAL AND FOREST METEOROLOGY}, author={Bucci, Sandra J. and Scholz, Fabian G. and Goldstein, Guillermo and Hoffmann, William A. and Meinzer, Frederick C. and Franco, Augusto C. and Giambelluca, Thomas and Miralles-Wilhelm, Fernando}, year={2008}, month={Jun}, pages={839–849} }
 @article{silva_sternberg_haridasan_hoffmann_miralles-wilhelm_franco_2008, title={Expansion of gallery forests into central Brazilian savannas}, volume={14}, ISSN={["1365-2486"]}, DOI={10.1111/j.1365-2486.2008.01637.x}, abstractNote={Abstract}, number={9}, journal={GLOBAL CHANGE BIOLOGY}, author={Silva, Lucas C. R. and Sternberg, Leonel and Haridasan, Mundayatan and Hoffmann, William A. and Miralles-Wilhelm, Fernando and Franco, Augusto C.}, year={2008}, month={Sep}, pages={2108–2118} }
 @article{hao_hoffmann_scholz_bucci_meinzer_franco_cao_goldstein_2008, title={Stem and leaf hydraulics of congeneric tree species from adjacent tropical savanna and forest ecosystems}, volume={155}, ISSN={["1432-1939"]}, DOI={10.1007/s00442-007-0918-5}, abstractNote={Leaf and stem functional traits related to plant water relations were studied for six congeneric species pairs, each composed of one tree species typical of savanna habitats and another typical of adjacent forest habitats, to determine whether there were intrinsic differences in plant hydraulics between these two functional types. Only individuals growing in savanna habitats were studied. Most stem traits, including wood density, the xylem water potential at 50% loss of hydraulic conductivity, sapwood area specific conductivity, and leaf area specific conductivity did not differ significantly between savanna and forest species. However, maximum leaf hydraulic conductance (K (leaf)) and leaf capacitance tended to be higher in savanna species. Predawn leaf water potential and leaf mass per area were also higher in savanna species in all congeneric pairs. Hydraulic vulnerability curves of stems and leaves indicated that leaves were more vulnerable to drought-induced cavitation than terminal branches regardless of genus. The midday K (leaf) values estimated from leaf vulnerability curves were very low implying that daily embolism repair may occur in leaves. An electric circuit analog model predicted that, compared to forest species, savanna species took longer for their leaf water potentials to drop from predawn values to values corresponding to 50% loss of K (leaf) or to the turgor loss points, suggesting that savanna species were more buffered from changes in leaf water potential. The results of this study suggest that the relative success of savanna over forest species in savanna is related in part to their ability to cope with drought, which is determined more by leaf than by stem hydraulic traits. Variation among genera accounted for a large proportion of the total variance in most traits, which indicates that, despite different selective pressures in savanna and forest habitats, phylogeny has a stronger effect than habitat in determining most hydraulic traits.}, number={3}, journal={OECOLOGIA}, author={Hao, Guang-You and Hoffmann, William A. and Scholz, Fabian G. and Bucci, Sandra J. and Meinzer, Frederick C. and Franco, Augusto C. and Cao, Kun-Fang and Goldstein, Guillermo}, year={2008}, month={Mar}, pages={405–415} }
 @article{hoffmann_haridasan_2008, title={The invasive grass, Melinis minutiflora, inhibits tree regeneration in a Neotropical savanna}, volume={33}, ISSN={["1442-9985"]}, DOI={10.1111/j.1442-9993.2007.01787.x}, abstractNote={Abstract  Exotic grasses are becoming increasingly abundant in Neotropical savannas, with Melinis minutiflora Beauv. being particularly invasive. To better understand the consequences for the native flora, we performed a field study to test the effect of this species on the establishment, survival and growth of seedlings of seven tree species native to the savannas and forests of the Cerrado region of Brazil. Seeds of the tree species were sown in 40 study plots, of which 20 were sites dominated by M. minutiflora, and 20 were dominated by native grasses. The exotic grass had no discernable effect on initial seedling emergence, as defined by the number of seedlings present at the end of the first growing season. Subsequent seedling survival in plots dominated by M. minutiflora was less than half that of plots dominated by native species. Consequently, at the end of the third growing season, invaded plots had only 44% as many seedlings as plots with native grasses. Above‐ground grass biomass of invaded plots was more than twice that of uninvaded plots, while seedling survival was negatively correlated with grass biomass, suggesting that competition for light may explain the low seedling survival where M. minutiflora is dominant. Soils of invaded plots had higher mean Ca, Mg and Zn, but these variables did not account for the higher grass biomass or the lower seedling survival in invaded plots. The results indicate that this exotic grass is having substantial effects on the dynamics of the tree community, with likely consequences for ecosystem structure and function.}, number={1}, journal={AUSTRAL ECOLOGY}, author={Hoffmann, William A. and Haridasan, M.}, year={2008}, month={Feb}, pages={29–36} }
 @article{goldstein_meinzer_bucci_scholz_franco_hoffmann_2008, title={Water economy of Neotropical savanna trees: six paradigms revisited}, volume={28}, ISSN={["1758-4469"]}, DOI={10.1093/treephys/28.3.395}, abstractNote={Biologists have long been puzzled by the striking morphological and anatomical characteristics of Neotropical savanna trees which have large scleromorphic leaves, allocate more than half of their total biomass to belowground structures and produce new leaves during the peak of the dry season. Based on results of ongoing interdisciplinary projects in the savannas of central Brazil (cerrado), we reassessed the validity of six paradigms to account for the water economy of savanna vegetation. (1) All savanna woody species are similar in their ability to take up water from deep soil layers where its availability is relatively constant throughout the year. (2) There is no substantial competition between grasses and trees for water resources during the dry season because grasses exclusively explore upper soil layers, whereas trees access water in deeper soil layers. (3) Tree species have access to abundant groundwater, their stomatal control is weak and they tend to transpire freely. (4) Savanna trees experience increased water deficits during the dry season despite their access to deep soil water. (5) Stomatal conductance of savanna species is low at night to prevent nocturnal transpiration, particularly during the dry season. (6) Savanna tree species can be classified into functional groups according to leaf phenology. We evaluated each paradigm and found differences in the patterns of water uptake between deciduous and evergreen tree species, as well as among evergreen tree species, that have implications for regulation of tree water balance. The absence of resource interactions between herbaceous and woody plants is refuted by our observation that herbaceous plants use water from deep soil layers that is released by deep-rooted trees into the upper soil layer. We obtained evidence of strong stomatal control of transpiration and show that most species exhibit homeostasis in maximum water deficit, with midday water potentials being almost identical in the wet and dry seasons. Although stomatal control is strong during the day, nocturnal transpiration is high during the dry season. Our comparative studies showed that the grouping of species into functional categories is somewhat arbitrary and that ranking species along continuous functional axes better represents the ecological complexity of adaptations of cerrado woody species to their seasonal environment.}, number={3}, journal={TREE PHYSIOLOGY}, author={Goldstein, Guillermo and Meinzer, Frederick C. and Bucci, Sandra J. and Scholz, Fabian G. and Franco, Augusto C. and Hoffmann, William A.}, year={2008}, month={Mar}, pages={395–404} }
 @article{lahoreau_barot_gignoux_hoffmann_setterfield_williams_2006, title={Positive effect of seed size on seedling survival in fire-prone savannas of Australia, Brazil and West Africa}, volume={22}, ISSN={["1469-7831"]}, DOI={10.1017/S026646740600349X}, abstractNote={All plant species face a fundamental reproductive trade-off: for a given investment in seed mass, they can produce either many small seeds or few large seeds. Whereas small seeds favour the germination of numerous seedlings, large seeds favour the survival of seedlings in the face of common stresses such as herbivory, drought or shade (Leishman et al. 2000). One mechanism explaining the better survival of large-seeded species is the seedling size effect (SSE) (Westoby et al. 1996): because seeds with large reserves result in bigger seedlings, seedlings from large-seeded species would have better access to light and/or to reliable water supply than seedlings from small-seeded species.}, journal={JOURNAL OF TROPICAL ECOLOGY}, author={Lahoreau, Gaelle and Barot, Sebastien and Gignoux, Jacques and Hoffmann, William A. and Setterfield, Samantha A. and Williams, Paul R.}, year={2006}, month={Nov}, pages={719–722} }
 @article{sternberg_bucci_franco_goldstein_hoffman_meinzer_moreira_scholz_2005, title={Long range lateral root activity by neo-tropical savanna trees}, volume={270}, ISSN={["0032-079X"]}, DOI={10.1007/s11104-004-1334-9}, number={1-2}, journal={PLANT AND SOIL}, author={Sternberg, LDL and Bucci, S and Franco, A and Goldstein, G and Hoffman, WA and Meinzer, FC and Moreira, MZ and Scholz, F}, year={2005}, month={Mar}, pages={169–178} }
 @article{hoffmann_silva_machado_bucci_scholz_goldstein_meinzer_2005, title={Seasonal leaf dynamics across a tree density gradient in a Brazilian savanna}, volume={145}, ISSN={["1432-1939"]}, DOI={10.1007/s00442-005-0129-x}, abstractNote={Interactions between trees and grasses that influence leaf area index (LAI) have important consequences for savanna ecosystem processes through their controls on water, carbon, and energy fluxes as well as fire regimes. We measured LAI, of the groundlayer (herbaceous and woody plants <1-m tall) and shrub and tree layer (woody plants >1-m tall), in the Brazilian cerrado over a range of tree densities from open shrub savanna to closed woodland through the annual cycle. During the dry season, soil water potential was strongly and positively correlated with grass LAI, and less strongly with tree and shrub LAI. By the end of the dry season, LAI of grasses, groundlayer dicots and trees declined to 28, 60, and 68% of mean wet-season values, respectively. We compared the data to remotely sensed vegetation indices, finding that field measurements were more strongly correlated to the enhanced vegetation index (EVI, r (2)=0.71) than to the normalized difference vegetation index (NDVI, r (2)=0.49). Although the latter has been more widely used in quantifying leaf dynamics of tropical savannas, EVI appears better suited for this purpose. Our ground-based measurements demonstrate that groundlayer LAI declines with increasing tree density across sites, with savanna grasses being excluded at a tree LAI of approximately 3.3. LAI averaged 4.2 in nearby gallery (riparian) forest, so savanna grasses were absent, thereby greatly reducing fire risk and permitting survival of fire-sensitive forest tree species. Although edaphic conditions may partly explain the larger tree LAI of forests, relative to savanna, biological differences between savanna and forest tree species play an important role. Overall, forest tree species had 48% greater LAI than congeneric savanna trees under similar growing conditions. Savanna and forest species play distinct roles in the structure and dynamics of savanna-forest boundaries, contributing to the differences in fire regimes, microclimate, and nutrient cycling between savanna and forest ecosystems.}, number={2}, journal={OECOLOGIA}, author={Hoffmann, WA and Silva, ER and Machado, GC and Bucci, SJ and Scholz, FG and Goldstein, G and Meinzer, FC}, year={2005}, month={Sep}, pages={307–316} }
 @article{hoffmann_franco_moreira_haridasan_2005, title={Specific leaf area explains differences in leaf traits between congeneric savanna and forest trees}, volume={19}, ISSN={["0269-8463"]}, DOI={10.1111/j.1365-2435.2005.01045.x}, abstractNote={Summary}, number={6}, journal={FUNCTIONAL ECOLOGY}, author={Hoffmann, WA and Franco, AC and Moreira, MZ and Haridasan, M}, year={2005}, month={Dec}, pages={932–940} }
 @article{hoffmann_orthen_franco_2004, title={Constraints to seedling success of savanna and forest trees across the savanna-forest boundary}, volume={140}, ISSN={["1432-1939"]}, DOI={10.1007/s00442-004-1595-2}, abstractNote={Tropical savannas and closed forests are characterized by distinct tree communities, with most species occurring almost exclusively in only one of the two environments. The ecology of these two groups of species will largely determine the structure and dynamics of the savanna-forest boundary, but little is known about the ecological and physiological differences that might control their distributions. We performed field and nursery experiments to compare seedling establishment success, predawn leaf water potential, biomass allocation, and root carbohydrate concentration of congeneric species, each composed of one savanna species and one forest species. Seedling establishment of savanna and forest species responded differently to vegetation cover, with forest species having lowest establishment success in the open savanna and savanna species having lowest success in forest. Subsequent survival followed similar patterns, resulting in even greater differences in cumulative success. The low survival of forest species in the savanna appears related to drought stress, as seedlings of forest species had lower predawn leaf water potential than savanna species. Seedlings of savanna species had greater root: shoot ratios and root total nonstructural carbohydrate (TNC) concentration, particularly among evergreen genera. Among evergreen genera, root TNC per shoot mass, which may largely determine resprout capacity, was seven times higher in savanna species than forest species. Although water availability and microclimate may reduce the success of forest species, these factors appear unable to completely exclude forest seedling establishment in savanna. Fire, on the other hand, appears to be a much more absolute constraint to success of forest species in savanna.}, number={2}, journal={OECOLOGIA}, author={Hoffmann, WA and Orthen, B and Franco, AC}, year={2004}, month={Jul}, pages={252–260} }
 @article{hoffmann_lucatelli_silva_azeuedo_marinho_albuquerque_lopes_moreira_2004, title={Impact of the invasive alien grass Melinis minutiflora at the savanna-forest ecotone in the Brazilian Cerrado}, volume={10}, ISSN={["1366-9516"]}, DOI={10.1111/j.1366-9516.2004.00063.x}, abstractNote={ABSTRACT}, number={2}, journal={DIVERSITY AND DISTRIBUTIONS}, author={Hoffmann, WA and Lucatelli, VMPC and Silva, FJ and Azeuedo, INC and Marinho, MD and Albuquerque, AMS and Lopes, AD and Moreira, SP}, year={2004}, month={Mar}, pages={99–103} }
 @article{hoffmann_orthen_do nascimento_2003, title={Comparative fire ecology of tropical savanna and forest trees}, volume={17}, ISSN={["1365-2435"]}, DOI={10.1111/j.1365-2435.2003.00796.x}, abstractNote={Summary}, number={6}, journal={FUNCTIONAL ECOLOGY}, author={Hoffmann, WA and Orthen, B and Do Nascimento, PKV}, year={2003}, month={Dec}, pages={720–726} }
 @article{hoffmann_schroeder_jackson_2003, title={Regional feedbacks among fire, climate, and tropical deforestation}, volume={108}, number={D23}, journal={Journal of Geophysical Research. Atmospheres}, author={Hoffmann, W. A. and Schroeder, W. and Jackson, R. B.}, year={2003} }