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Works (16)
Updated: October 21st, 2024 08:34
2024 journal article
Mixed-mode size-exclusion silica resin for polishing human antibodies in flow-through mode
Journal of Chromatography A, 1720, 464772.
author keywords: Antibody fragments; Product polishing; Mixed -mode ligands; Silica resin; Flow -through chromatography
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 29, 2024
2024 journal article
Peptide ligands for the affinity purification of adenovirus from HEK293 and vero cell lysates
JOURNAL OF CHROMATOGRAPHY A, 1736.
author keywords: Affinity chromatography; Adenovirus; Viral vectors; Gene therapy; Peptide ligands
Sources: Web Of Science, NC State University Libraries
Added: October 14, 2024
2024 article
Peptide ligands for the universal purification of exosomes by affinity chromatography
Kilgore, R. E., Moore, B. D., Sripada, S. A., Chu, W., Shastry, S., Barbieri, E., … Menegatti, S. (2024, August 4). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 8.
author keywords: affinity chromatography; bioseparations; exosomes; gene delivery; peptide ligands
open access via doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 14, 2024
2024 journal article
Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins
JOURNAL OF CHROMATOGRAPHY A, 1734.
author keywords: Gene therapy; Adeno-associated virus; Peptide ligands; Affinity chromatography; Transduction activity
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 1, 2024
2023 journal article
Molecular Engineering of Cyclic Azobenzene‐Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo‐Affinity Chromatography
Advanced Functional Materials, 33(14).
author keywords: affinity ligands; azobenzene-peptide hybrids; biomolecular recognition; factor VIII; photo-affinity chromatography
TL;DR:
The cyclic azobenzene‐peptide ligands introduced in this report demonstrate a novel route integrating gentle operational conditions in a rapid and efficient bioprocess for the purification of life‐saving biotherapeutics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: February 20, 2023
2023 article
Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates
Chu, W., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, July 12). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 7.
author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
TL;DR:
Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 7, 2023
2023 article
Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography
Shastry, S., Chu, W., Barbieri, E., Greback-Clarke, P., Smith, W. K., Cummings, C., … Menegatti, S. (2023, September 28). BIOTECHNOLOGY JOURNAL, Vol. 9.
author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
TL;DR:
This study introduces a cohort of peptide ligands that mimic the biorecognition activity of the AAV receptor (AAVR) and anti-AAV antibody A20, enable product elution under near-physiological conditions (pH 6.0) and grant extended reusability by withstanding multiple regenerations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 10, 2023
2023 journal article
The downstream bioprocess toolbox for therapeutic viral vectors
JOURNAL OF CHROMATOGRAPHY A, 1709.
author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
TL;DR:
This study presents a comprehensive overview of the downstream bioprocess toolbox for viral vectors established in the last decade, and discusses present efforts and future directions contributing to the success of this promising class of biological medicines.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023
2023 article
Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy
2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS.
author keywords: adeno-associated virus; AAV2; immunosensor; electrochemical impedance spectroscopy; viral vector
TL;DR:
The preliminary development of an immunosensor for measuring the titer of adeno-associated virus 2 (AAV2), which may be deployed for rapid quantification of product yield during AAV biomanufacturing.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 4, 2023
2022 journal article
<p>De novo discovery of peptide-based affinity ligands for the fab fragment of human immunoglobulin G</p>
JOURNAL OF CHROMATOGRAPHY A, 1669.
author keywords: Affinity ligands; Ligand design; Biorecognition; Protein chromatography; Fragment antigen binding
MeSH headings : Humans; Immunoglobulin Fab Fragments / chemistry; Immunoglobulin G; Ligands; Peptides; Tissue Distribution
TL;DR:
An integrated computational and experimental scheme is described to de novo design 9-mer peptides that bind to Fab fragments that lay the groundwork for future development of these ligands towards biomanufacturing translation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 2, 2022
2022 journal article
Development of peptide affinity ligands for the purification of polyclonal and monoclonal Fabs from recombinant fluids
JOURNAL OF CHROMATOGRAPHY A, 1687.
author keywords: Peptide ligands; Affinity chromatography; Fragment antigen binding; Monoclonal antibodies; Cell culture harvests
MeSH headings : Cricetinae; Animals; Humans; Cricetulus; Ligands; CHO Cells; Molecular Docking Simulation; Protein Binding; Peptides / chemistry; Antibodies, Monoclonal; Chromatography, Affinity
TL;DR:
The de novo discovery of peptide ligands that target different regions of human Fab and enable product release under mild conditions are discovered and afforded values of yield and purity comparable to those provided by Protein L resins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 17, 2023
2022 journal article
Development of peptide ligands for the purification of a-1 antitrypsin from cell culture fluids
JOURNAL OF CHROMATOGRAPHY A, 1679.
author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022
2022 article
Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents
Sripada, S. A., Chu, W., Williams, T. I., Teten, M. A., Mosley, B. J., Carbonell, R. G., … Menegatti, S. (2022, April 22). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 119.
Contributors: S. Sripada n, n, T. Williams n , M. Teten n, B. Mosley n, R. Carbonell n, A. Lenhoff *, S. Cramer *
author keywords: CHO; flow-through chromatography; host cell proteins; monoclonal antibodies; peptide-based adsorbents
MeSH headings : Animals; Antibodies, Monoclonal / chemistry; CHO Cells; Cell Culture Techniques; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Peptides / chemistry; Proteomics / methods
TL;DR:
An ensemble of peptide ligands that target the HCPs in Chinese hamster ovary (CHO) cell culture fluids and enable mAb purification via flow‐through affinity chromatography are introduced and their integration into LigaGuard™, an affinity adsorbent featuring an equilibrium binding capacity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: April 10, 2022
2021 article
Purification of polyclonal immunoglobulin G from human serum using peptide-based adsorbents
Chu, W., Sripada, S. A., Reese, H. R., Bhandari, D., Adams, A., Sly, J., … Menegatti, S. (2021, October 20). AICHE JOURNAL, Vol. 10.
author keywords: affinity ligands; LigaGuard (TM); LigaTrap (TM); polyclonal antibodies; protein chromatography
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 1, 2021
2020 journal article
Peptides and pseudopeptide ligands: a powerful toolbox for the affinity purification of current and next-generation biotherapeutics
JOURNAL OF CHROMATOGRAPHY A, 1635.
author keywords: Affinity ligands; peptides; peptoids; pseudopeptides; biotherapeutics
MeSH headings : Antibodies / isolation & purification; Biological Products / isolation & purification; Chemistry, Pharmaceutical / methods; Chromatography, Affinity; Family Characteristics; Humans; Ligands; Peptides / isolation & purification; Peptoids / chemistry; Proteins / isolation & purification
TL;DR:
This work presents a comprehensive and systematic review of the literature on peptide-based ligands and their use in the affinity purification of established and upcoming biological drugs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 25, 2021
2020 journal article
Removal of host cell proteins from cell culture fluids by weak partitioning chromatography using peptide-based adsorbents
SEPARATION AND PURIFICATION TECHNOLOGY, 257.
Contributors: R. Lavoie n, n, J. Lavoie n, Z. Hetzler n, T. Williams n , R. Carbonell n, S. Menegatti n
author keywords: Host cell proteins; Monoclonal antibodies; Flow-through chromatography; Weak partitioning mode; Polishing; Proteomics; Peptide ligands
TL;DR:
Proteomic analysis of the effluents demonstrated that 6HP+4MP-Toyopearl adsorbent removes HCPs known for their immunogenicity or IgG co-elution or degradation, demonstrating the potential of these peptide-based resins as HCP scrubbers in mAb purification processes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 11, 2021
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