Wenning Chu

Works (12)

Updated: December 6th, 2023 05:00
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2023 journal article

Development of peptide affinity ligands for the purification of polyclonal and monoclonal Fabs from recombinant fluids

JOURNAL OF CHROMATOGRAPHY A, 1687.

By: R. Kilgore n, W. Chu n, D. Bhandari*, D. Fischler*, R. Carbonell n, M. Crapanzano*, S. Menegatti n 

co-author countries: United States of America 🇺🇸
author keywords: Peptide ligands; Affinity chromatography; Fragment antigen binding; Monoclonal antibodies; Cell culture harvests
MeSH headings : Cricetinae; Animals; Humans; Cricetulus; Ligands; CHO Cells; Molecular Docking Simulation; Protein Binding; Peptides / chemistry; Antibodies, Monoclonal; Chromatography, Affinity
Sources: Web Of Science, ORCID
Added: January 17, 2023

2023 article

Molecular Engineering of Cyclic Azobenzene-Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo-Affinity Chromatography

Prodromou, R., Moore, B. D., Chu, W., Deal, H., San Miguel, A., Brown, A. C., … Menegatti, S. (2023, January 25). ADVANCED FUNCTIONAL MATERIALS, Vol. 1.

By: R. Prodromou n, B. Moore n, W. Chu n, H. Deal n, A. San Miguel n , A. Brown n, M. Daniele n, V. Pozdin*, S. Menegatti n 

co-author countries: United States of America 🇺🇸
author keywords: affinity ligands; azobenzene-peptide hybrids; biomolecular recognition; factor VIII; photo-affinity chromatography
Sources: Web Of Science, ORCID
Added: February 20, 2023

2023 article

Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

Chu, W., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, July 12). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 7.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

co-author countries: United States of America 🇺🇸
author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
Sources: Web Of Science, ORCID
Added: August 7, 2023

2023 article

Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography

Shastry, S., Chu, W., Barbieri, E., Greback-Clarke, P., Smith, W. K., Cummings, C., … Menegatti, S. (2023, September 28). BIOTECHNOLOGY JOURNAL, Vol. 9.

By: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback-Clarke n, W. Smith n, C. Cummings n, A. Minzoni n, J. Pancorbo n ...

co-author countries: United Kingdom of Great Britain and Northern Ireland 🇬🇧 United States of America 🇺🇸
author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
Sources: Web Of Science, ORCID
Added: October 10, 2023

2023 journal article

The downstream bioprocess toolbox for therapeutic viral vectors

JOURNAL OF CHROMATOGRAPHY A, 1709.

By: R. Kilgore n, A. Minzoni n, S. Shastry n, W. Smith n, E. Barbieri n, Y. Wu n, J. Lebarre n, W. Chu n, J. O'Brien n, S. Menegatti n 

co-author countries: United States of America 🇺🇸
author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
Sources: Web Of Science, ORCID
Added: October 30, 2023

2023 article

Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy

2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS.

By: J. Wang, M. Hosseini, S. Shastry, E. Barbieri, W. Chu, S. Menegatti , M. Daniele

author keywords: adeno-associated virus; AAV2; immunosensor; electrochemical impedance spectroscopy; viral vector
Sources: Web Of Science, ORCID
Added: December 4, 2023

2022 journal article

De novo discovery of peptide-based affinity ligands for the fab fragment of human immunoglobulin G

JOURNAL OF CHROMATOGRAPHY A, 1669.

By: X. Xiao n, R. Kilgore n, S. Sarma n, W. Chu n, S. Menegatti n  & C. Hall n 

co-author countries: United States of America 🇺🇸
author keywords: Affinity ligands; Ligand design; Biorecognition; Protein chromatography; Fragment antigen binding
MeSH headings : Humans; Immunoglobulin Fab Fragments / chemistry; Immunoglobulin G; Ligands; Peptides; Tissue Distribution
Sources: Web Of Science, ORCID
Added: May 2, 2022

2022 journal article

Development of peptide ligands for the purification of a-1 antitrypsin from cell culture fluids

JOURNAL OF CHROMATOGRAPHY A, 1679.

By: W. Chu n, R. Prodromou n, B. Moore n, D. Elhanafi n, R. Kilgore n, S. Shastry n, S. Menegatti n 

co-author countries: United States of America 🇺🇸
author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
Sources: Web Of Science, ORCID
Added: October 11, 2022

2022 article

Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents

Sripada, S. A., Chu, W., Williams, T. I., Teten, M. A., Mosley, B. J., Carbonell, R. G., … Menegatti, S. (2022, April 22). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 119.

By: S. Sripada n, W. Chu n, T. Williams n , M. Teten n, B. Mosley n, R. Carbonell n, A. Lenhoff *, S. Cramer * ...

co-author countries: United States of America 🇺🇸

Contributors: S. Sripada n, W. Chu n, T. Williams n , M. Teten n, B. Mosley n, R. Carbonell n, A. Lenhoff *, S. Cramer * ...

author keywords: CHO; flow-through chromatography; host cell proteins; monoclonal antibodies; peptide-based adsorbents
MeSH headings : Animals; Antibodies, Monoclonal / chemistry; CHO Cells; Cell Culture Techniques; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Peptides / chemistry; Proteomics / methods
Sources: Web Of Science, ORCID
Added: April 10, 2022

2021 journal article

Peptides and pseudopeptide ligands: a powerful toolbox for the affinity purification of current and next-generation biotherapeutics

JOURNAL OF CHROMATOGRAPHY A, 1635.

By: W. Chu n, R. Prodromou n, K. Day n, J. Schneible n, K. Bacon n, J. Bowen n, R. Kilgore n, C. Catella n ...

co-author countries: China 🇨🇳 United States of America 🇺🇸
author keywords: Affinity ligands; peptides; peptoids; pseudopeptides; biotherapeutics
MeSH headings : Antibodies / isolation & purification; Biological Products / isolation & purification; Chemistry, Pharmaceutical / methods; Chromatography, Affinity; Family Characteristics; Humans; Ligands; Peptides / isolation & purification; Peptoids / chemistry; Proteins / isolation & purification
Sources: Web Of Science, ORCID
Added: January 25, 2021

2021 article

Purification of polyclonal immunoglobulin G from human serum using peptide-based adsorbents

Chu, W., Sripada, S. A., Reese, H. R., Bhandari, D., Adams, A., Sly, J., … Menegatti, S. (2021, October 20). AICHE JOURNAL, Vol. 10.

By: W. Chu n, S. Sripada n, H. Reese n, D. Bhandari, A. Adams n, J. Sly, M. Crapanzano, S. Menegatti n 

co-author countries: United States of America 🇺🇸
author keywords: affinity ligands; LigaGuard (TM); LigaTrap (TM); polyclonal antibodies; protein chromatography
Sources: Web Of Science, ORCID
Added: November 1, 2021

2021 journal article

Removal of host cell proteins from cell culture fluids by weak partitioning chromatography using peptide-based adsorbents

SEPARATION AND PURIFICATION TECHNOLOGY, 257.

By: R. Lavoie n, W. Chu n, J. Lavoie n, Z. Hetzler n, T. Williams n , R. Carbonell n, S. Menegatti n 

co-author countries: United States of America 🇺🇸

Contributors: R. Lavoie n, W. Chu n, J. Lavoie n, Z. Hetzler n, T. Williams n , R. Carbonell n, S. Menegatti n 

author keywords: Host cell proteins; Monoclonal antibodies; Flow-through chromatography; Weak partitioning mode; Polishing; Proteomics; Peptide ligands
Sources: Web Of Science, ORCID
Added: January 11, 2021