William F. Thompson

Also known as: Bill Thompson, W. F. Thompson, William F. Thompson

Plant genomics, gene expression, transformation, chromatin, DNA replication

Works (178)

Updated: April 6th, 2024 12:15

2021 journal article

A protocol for genome-wide analysis of DNA replication timing in intact root tips

Methods in Molecular Biology Series.

By: L. Mickelson-Young, E. Wear, J. Song, G. Zynda, L. Hanley-Bowdoin & W. Thompson

Source: NC State University Libraries
Added: December 18, 2020

2021 journal article

Loss of Small-RNA-Directed DNA Methylation in the Plant Cell Cycle Promotes Germline Reprogramming and Somaclonal Variation

CURRENT BIOLOGY, 31(3), 591-+.

MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Cell Cycle; Cytosine; DNA Methylation; DNA, Plant; Gene Expression Regulation, Plant; Germ Cells / metabolism; Histones / genetics; Histones / metabolism; Plants / metabolism; RNA, Small Interfering / genetics
TL;DR: Comparative analysis with methylomes of various tissues and cell types suggests that loss of small-RNA-directed non-CG methylation during DNA replication promotes germline reprogramming and epigenetic variation in plants propagated as clones. (via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: December 4, 2020

2020 journal article

Arabidopsis DNA Replication Initiates in Intergenic, AT-Rich Open Chromatin(1)([OPEN])

PLANT PHYSIOLOGY, 183(1), 206–220.

By: E. Wheeler n, A. Brooks n, L. Concia n, D. Vera*, E. Wear n, C. LeBlanc*, U. Ramu*, M. Vaughn ...

MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Chromatin / metabolism; DNA Replication / genetics; DNA Replication / physiology; DNA, Plant / metabolism; DNA, Plant / physiology; Replication Origin / genetics; Replication Origin / physiology
TL;DR: A new functional assay to label and map very early replicating loci that must, by definition, include at least a subset of replication origins, and indicates that IRs are associated with accessible chromatin. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 15, 2020

2020 journal article

Comparing DNA replication programs reveals large timing shifts at centromeres of endocycling cells in maize roots

PLOS GENETICS, 16(10).

By: E. Wear n, J. Song, G. Zynda, L. Mickelson-Young n, C. LeBlanc*, T. Lee n, D. Deppong n, G. Allen n ...

MeSH headings : Cell Nucleus / drug effects; Cell Nucleus / genetics; Centromere / drug effects; Centromere / genetics; DNA Replication / drug effects; DNA Replication / genetics; DNA Replication Timing / drug effects; DNA Replication Timing / genetics; DNA, Plant / drug effects; DNA, Plant / genetics; Deoxyuridine / analogs & derivatives; Deoxyuridine / pharmacology; Endocytosis / drug effects; Meristem / drug effects; Meristem / genetics; Mitosis / drug effects; Mitosis / genetics; Nucleosomes / drug effects; Plant Roots / drug effects; Plant Roots / genetics; Plant Roots / growth & development; S Phase / genetics; Zea mays / genetics; Zea mays / growth & development
Sources: Web Of Science, NC State University Libraries
Added: November 9, 2020

2018 article

Chromatin structure profile data from DNS-seq: Differential nuclease sensitivity mapping of four reference tissues of B73 maize (Zea mays L)

Turpin, Z. M., Vera, D. L., Savadel, S. D., Lung, P.-Y., Wear, E. E., Mickelson-Young, L., … Bass, H. W. (2018, October). DATA IN BRIEF, Vol. 20, pp. 358–363.

By: Z. Turpin*, D. Vera*, S. Savadel*, P. Lung*, E. Wear n, L. Mickelson-Young n, W. Thompson n, L. Hanley-Bowdoin n ...

TL;DR: Data from Next-Generation Sequencing of differential micrococcal nuclease digestions of formaldehyde-crosslinked chromatin in selected tissues of maize (Zea mays) inbred line B73 are presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 3, 2018

2018 journal article

Genome-Wide Analysis of the Arabidopsis Replication Timing Program

PLANT PHYSIOLOGY, 176(3), 2166–2185.

By: L. Concia n, A. Brooks n, E. Wheeler n, G. Zynda, E. Wear n, C. LeBlanc*, J. Song, T. Lee n ...

MeSH headings : Arabidopsis / genetics; Chromatin / genetics; Chromatin / metabolism; Chromosomes, Plant; DNA Replication Timing; DNA Transposable Elements; Flow Cytometry; Genome, Plant; Genome-Wide Association Study; S Phase / genetics; Sequence Analysis, DNA / methods
TL;DR: The thymidine analog, 5-ethynyl-2′-deoxyuridine, is used in combination with flow sorting and Repli-Seq to describe the genome-wide replication timing program for Arabidopsis (Arabidopsis thaliana) Col-0 suspension cells, and genomic regions that replicate predominantly during early, mid, and late S phase are identified. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 journal article

Genomic Analysis of the DNA Replication Timing Program during Mitotic S Phase in Maize (Zea mays) Root Tips

PLANT CELL, 29(9), 2126–2149.

By: E. Wear n, J. Song, G. Zynda, C. LeBlanc*, T. Lee n, L. Mickelson-Young n, L. Concia n, P. Mulvaney n ...

MeSH headings : Base Sequence; Chromosomes, Plant / genetics; DNA Replication Timing / genetics; DNA Transposable Elements / genetics; Genes, Plant; Genomics; Meristem / cytology; Meristem / genetics; Mitosis / genetics; Models, Genetic; S Phase / genetics; Tandem Repeat Sequences / genetics; Time Factors; Transcription, Genetic; Zea mays / cytology; Zea mays / genetics
TL;DR: A novel adaptation of the “Repli-seq” assay for use in intact root tips of maize (Zea mays) that includes several different cell lineages and present whole-genome replication timing profiles from cells in early, mid, and late S phase of the mitotic cell cycle is reported. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 journal article

Repliscan: a tool for classifying replication timing regions

BMC BIOINFORMATICS, 18, 1–14.

By: G. Zynda, J. Song, L. Concia n, E. Wear n, L. Hanley-Bowdoin n, W. Thompson n, M. Vaughn

author keywords: DNA replication; Repli-seq; Classification
MeSH headings : DNA Replication Timing; Genome; Genome Size; High-Throughput Nucleotide Sequencing / methods; Sequence Analysis, DNA / methods; Software
TL;DR: To accurately detect and classify regions of replication across the genome, Repliscan is presented, which robustly normalizes, automatically removes outlying and uninformative data points, and classifies Repli-seq signals into discrete combinations of replication signatures. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 journal article

A flow cytometric method for estimating S-phase duration in plants

JOURNAL OF EXPERIMENTAL BOTANY, 67(21), 6077–6087.

By: L. Mickelson-Young n, E. Wear n, P. Mulvaney n, T. Lee n, E. Szymanski n, G. Allen n, L. Hanley-Bowdoin n, W. Thompson n

author keywords: Arabidopsis; barley; EdU; flow cytometry; maize; replication timing; rice; S-phase duration; wheat
MeSH headings : Arabidopsis / cytology; Arabidopsis / growth & development; DNA, Plant / metabolism; Deoxyuridine / analogs & derivatives; Deoxyuridine / metabolism; Flow Cytometry / methods; G1 Phase / physiology; G2 Phase / physiology; Hordeum / cytology; Hordeum / growth & development; Meristem / cytology; Meristem / growth & development; Oryza / cytology; Oryza / growth & development; S Phase / physiology; Triticum / cytology; Triticum / growth & development; Zea mays / cytology; Zea mays / growth & development
TL;DR: This work estimated S-phase duration for several plant species by following EdU-labeled nuclei from G1 to G2 using bivariate flow cytometry, finding that S- phase duration is relatively consistent over a range of genome sizes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 chapter

Isolation of Plant Nuclei at Defined Cell Cycle Stages Using EdU Labeling and Flow Cytometry

In Methods in Molecular Biology (pp. 69–86).

By: E. Wear n, L. Concia n, A. Brooks n, E. Markham n, T. Lee n, G. Allen n, W. Thompson n, L. Hanley-Bowdoin n

author keywords: 5-Ethynyl-2 '-deoxyuridine; Flow cytometry; Nuclei sorting; Arabidopsis; Maize; Percoll gradient; Cell cycle; DNA replication; Chromatin
MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Cell Cycle; Cell Fractionation / methods; Cell Nucleus / genetics; Click Chemistry / methods; DNA Replication; DNA, Plant / analysis; DNA, Plant / genetics; Deoxyuridine / analogs & derivatives; Deoxyuridine / analysis; Flow Cytometry / methods; Fluorescent Dyes / analysis; Zea mays / cytology; Zea mays / genetics
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: ORCID, Crossref, NC State University Libraries
Added: February 14, 2019

2015 journal article

Defining multiple, distinct, and shared spatiotemporal patterns of DNA replication and endoreduplication from 3D image analysis of developing maize (Zea mays L.) root tip nuclei

PLANT MOLECULAR BIOLOGY, 89(4-5), 339–351.

By: H. Bass*, G. Hoffman*, T. Lee n, E. Wear n, S. Joseph*, G. Allen n, L. Hanley-Bowdoin n, W. Thompson n

author keywords: Cell cycle; DNA synthesis; Grass
MeSH headings : Cell Nucleus / genetics; Cell Nucleus / metabolism; DNA Replication / genetics; DNA Replication Timing / genetics; DNA, Plant / biosynthesis; DNA, Plant / genetics; Endoreduplication / genetics; Genes, Plant; Imaging, Three-Dimensional; Meristem / genetics; Meristem / growth & development; Meristem / metabolism; Models, Biological; S Phase / genetics; Zea mays / genetics; Zea mays / growth & development; Zea mays / metabolism
TL;DR: 3D quantitative analysis of DNA replication and endoreduplication in nuclei from pulse-labeled developing maize root tips revealed that maize euchromatin exists as an intermingled mixture of two components distinguished by their condensation state and replication timing. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 review

A maize root tip system to study DNA replication programmes in somatic and endocycling nuclei during plant development

[Review of ]. JOURNAL OF EXPERIMENTAL BOTANY, 65(10), 2747–2756.

By: H. Bass*, E. Wear n, T. Lee n, G. Hoffman*, H. Gumber*, G. Allen n, W. Thompson n, L. Hanley-Bowdoin n

author keywords: DNA replication; EdU; endocycle; flow cytometry; high-resolution microscopy; maize; mitotic cell cycle; root development; S phase
MeSH headings : Base Sequence; Cell Nucleus / genetics; DNA Replication; DNA, Plant / biosynthesis; In Situ Hybridization, Fluorescence; Oligonucleotide Probes; Plant Roots / growth & development; Zea mays / genetics; Zea mays / growth & development
TL;DR: A promising new system that can be used to visualize DNA replication in isolated maize root tip nuclei after in planta pulse labelling with the thymidine analogue, 5-ethynyl-2'-deoxyuridine (EdU). (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 journal article

In Vivo Mapping of Arabidopsis Scaffold/Matrix Attachment Regions Reveals Link to Nucleosome-Disfavoring Poly(dA:dT) Tracts

PLANT CELL, 26(1), 102–120.

By: P. Pascuzzi n, M. Flores-Vergara n, T. Lee n, B. Sosinski n, M. Vaughn, L. Hanley-Bowdoin n, W. Thompson n, G. Allen n

MeSH headings : Arabidopsis / genetics; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / physiology; Chromatin / metabolism; Gene Expression Regulation, Plant; Matrix Attachment Regions; Nucleosomes / metabolism; Nucleotide Motifs; Poly dA-dT / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription Factors / physiology
TL;DR: This work uses tiling microarrays to map S/MARs on Arabidopsis chromosome 4, found to be spaced more closely than in the large plant and animal genomes studied to date and preferentially enriched in poly(dA:dT) tracts, sequences that resist nucleosome formation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Starch Self-Processing in Transgenic Sweet Potato Roots Expressing a Hyperthermophilic alpha-Amylase

BIOTECHNOLOGY PROGRESS, 27(2), 351–359.

By: M. Santa-Maria n, C. Yencho n, C. Haigler n, W. Thompson n, R. Kelly n & B. Sosinski n

author keywords: hyperthermophilic enzymes; starch conversion; transgenic plants; sweet potato; biofuels
MeSH headings : Crops, Agricultural / genetics; Hot Temperature; Ipomoea batatas / genetics; Ipomoea batatas / metabolism; Plant Roots / metabolism; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / metabolism; Southeastern United States; Starch / metabolism; Thermotoga maritima / enzymology; alpha-Amylases / genetics; alpha-Amylases / physiology
TL;DR: The results presented here demonstrate that engineering plants with hyperthermophilic glycoside hydrolases can facilitate cost effective starch conversion to fermentable sugars and the use of sweet potato as an alternative near‐term energy crop should be considered. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2010 journal article

Arabidopsis thaliana Chromosome 4 Replicates in Two Phases That Correlate with Chromatin State

PLoS Genetics, 6(6), e1000982.

By: T. Lee n, P. Pascuzzi n, S. Settlage n, R. Shultz n, M. Tanurdzic*, P. Rabinowicz*, M. Menges*, P. Zheng* ...

Ed(s): G. Copenhaver

MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Chromatin / genetics; Chromosomes, Plant; DNA Replication; Epigenesis, Genetic; Flow Cytometry; Oligonucleotide Array Sequence Analysis; Replicon; S Phase
TL;DR: The similarity of the replication profiles for early and mid S phase cells indicates that replication origin activation in euchromatin is stochastic, and distinct patterns of association of H3K56ac with gene expression and early replication provide evidence that H3k56ac may be associated with initiation zones and replication origins. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref, NC State University Libraries
Added: August 6, 2018

2009 journal article

Dynamic Localization of the DNA Replication Proteins MCM5 and MCM7 in Plants

PLANT PHYSIOLOGY, 150(2), 658–669.

By: R. Shultz n, T. Lee n, G. Allen n, W. Thompson n & L. Hanley-Bowdoin n

MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Cell Cycle; Cell Cycle Proteins / metabolism; Cell Nucleus / metabolism; Chromatin / metabolism; DNA Replication; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Minichromosome Maintenance Complex Component 7; Multiprotein Complexes / genetics; Multiprotein Complexes / metabolism; Origin Recognition Complex / metabolism; Plant Roots / cytology; Plant Roots / metabolism; Protein Binding; Protein Subunits / genetics; Protein Subunits / metabolism; Protein Transport; Tobacco / cytology; Tobacco / metabolism
TL;DR: It is demonstrated that subunits of the MCM2-7 complex are coordinately expressed during Arabidopsis (Arabidopsis thaliana) development and are abundant in proliferating and endocycling tissues, indicative of a role in DNA replication. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2009 chapter

Plant Genome Engineering Using Zinc Finger Nucleases

In Molecular Techniques in Crop Improvement (pp. 579–590).

By: S. Kumar & W. Thompson*

TL;DR: ZFN-mediated gene targeting is likely to become a powerful technique for precise modification and directed mutagenesis of plant and mammalian genomes. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

2009 journal article

Plant cell calcium-rich environment enhances thermostability of recombinantly produced α-amylase from the hyperthermophilic bacterium Thermotoga maritime

Biotechnology and Bioengineering, 104(5), 947–956.

By: M. Santa-Maria n, C. Chou n, G. Yencho n, C. Haigler n, W. Thompson n, R. Kelly n, B. Sosinski n

author keywords: hyperthermophilic enzymes; starch processing; transgenic plants; tobacco NT1 Cell cultures; biofuel
MeSH headings : Calcium / pharmacology; Coenzymes / pharmacology; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Hot Temperature; Plants, Genetically Modified / enzymology; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Tobacco / enzymology; Tobacco / genetics; alpha-Amylases / chemistry; alpha-Amylases / genetics; alpha-Amylases / metabolism
TL;DR: In a proof of concept study, tobacco cell cultures were used as model system to test in planta production of a hyperthermophilic α‐amylase from Thermotoga maritima, demonstrating the importance of a calcium‐rich environment for thermostability, as well as the advantage of producing this enzyme directly in plant cells where calcium is readily available. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref, NC State University Libraries
Added: August 6, 2018

2009 journal article

Simultaneous excision of two transgene flanking sequences and resolution of complex integration loci

PLANT MOLECULAR BIOLOGY, 69(1-2), 23–32.

By: S. Kumar n & W. Thompson n

author keywords: Site specific recombination; Sequence excision; Complex transgene locus resolution; Transgene expression; Plant transformation
MeSH headings : Electrophoresis, Polyacrylamide Gel; Escherichia coli / genetics; Genetic Vectors; Plants, Genetically Modified; Recombination, Genetic; Transgenes
TL;DR: It is shown that a single SSR reaction can simultaneously carry out double excisions and resolve complex transgene loci at high frequency. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Epigenomic Consequences of Immortalized Plant Cell Suspension Culture

PLoS Biology, 6(12), e302.

By: M. Tanurdzic*, M. Vaughn*, H. Jiang*, T. Lee n, R. Slotkin*, B. Sosinski n, W. Thompson n, R. Doerge*, R. Martienssen*

Ed(s): W. Reik

MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis / growth & development; Base Sequence; Cells, Cultured; Chromatin Immunoprecipitation; Chromosomes, Plant / physiology; DNA Methylation; DNA Transposable Elements / genetics; Epigenesis, Genetic / genetics; Gene Expression; Genes, Plant / genetics; Genome, Plant; Histones / metabolism; Plant Leaves / chemistry; RNA, Plant / chemistry; RNA, Small Interfering / metabolism
TL;DR: It is found that euchromatin becomes hypermethylated in culture and that a small percentage of the hyper methylated genes become associated with heterochromatic marks. (via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: February 14, 2019

2008 review

Epigenomic consequences of immortalized plant cell suspension culture

[Review of ]. PLoS Biology, 6(12), 2880–2895.

By: M. Tanurdzic, M. Vaughn, H. Jiang, T. Lee, R. Slotkin, B. Sosinski, W. Thompson, R. Doerge, R. Martienssen

Source: NC State University Libraries
Added: August 6, 2018

2007 review

Genome-wide analysis of the core DNA replication machinery in the higher plants Arabidopsis and rice(1[W][OA])

[Review of ]. Plant Physiology, 144(4), 1697–1714.

By: R. Shultz, V. Tatineni, L. Hanley-Bowdoin & W. Thompson

Source: NC State University Libraries
Added: August 6, 2018

2006 journal article

A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide

NATURE PROTOCOLS, 1(5), 2320–2325.

By: G. Allen n, M. Flores-Vergara n, S. Krasnyanski, S. Kumar n & W. Thompson n

MeSH headings : Cetrimonium; Cetrimonium Compounds; DNA, Plant / isolation & purification; Magnoliopsida / chemistry; Plant Leaves / chemistry; Time Factors
TL;DR: The modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 review

Gene targeting in plants: fingers on the move

[Review of ]. TRENDS IN PLANT SCIENCE, 11(4), 159–161.

By: S. Kumar n, G. Allen n & W. Thompson n

MeSH headings : Chromatin Assembly and Disassembly; DNA Repair; Endodeoxyribonucleases / chemistry; Endodeoxyribonucleases / metabolism; Gene Targeting / methods; Genome, Plant; Plants / genetics; Recombination, Genetic; Zinc Fingers / physiology
TL;DR: Zinc-finger endonucleases can be harnessed to stimulate homologous recombination and gene targeting in plants, which represents a major step towards modifying the plant genome more precisely. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 patent

High efficiency gene targeting in plants

Washington, DC: U.S. Patent and Trademark Office.

By: G. Helmer, G. Allen & W. Thompson

Source: NC State University Libraries
Added: August 6, 2018

2006 journal article

In situ methods to localize transgenes and transcripts in interphase nuclei: a tool for transgenic plant research

Plant Methods, 2, 18.

By: A. Santos*, E. Wegel*, G. Allen n, W. Thompson n, E. Stoger*, P. Shaw*, R. Abranches*

TL;DR: In situ methods to visualize transgenes and their transcripts during interphase from different tissues and plant species are developed, which reduce the time necessary for characterization of transgene integration by eliminating the need for time-consuming segregation analysis, and extend characterization to the interphase nucleus, thus increasing the likelihood of accurate prediction of transGene activity. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: April 21, 2019

2005 journal article

A trichloroacetic acid-acetone method greatly reduces infrared autofluorescence of protein extracts from plant tissue

PLANT MOLECULAR BIOLOGY REPORTER, 23(4), 405–409.

By: R. Shultz n, S. Settlage n, L. Hanley-Bowdoin n & W. Thompson n

author keywords: Arabidopsis; autofluorescence; immunoblot; infrared; Odyssey; plant
TL;DR: A trichloroacetic acid-acetone method is reported that effectively eliminates autofluorescence while retaining the immunogenicity of a target protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Matrix attachment regions and regulated transcription increase and stabilize transgene expression

PLANT BIOTECHNOLOGY JOURNAL, 3(5), 535–543.

By: R. Abranches n, R. Shultz n, W. Thompson n & G. Allen n

author keywords: PTGS; TGS; RNAi; MARs; transgene expression; induction
TL;DR: Transgene silencing has been shown to be associated with strong promoters, but it is not known whether the propensity for silencing is caused by the level of transcription, or some other property of the promoter, so transgenes with inducible promoters may be less susceptible to silencing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to Tomato Spotted Wilt Virus in transgenic tobacco

TRANSGENIC RESEARCH, 14(2), 193–206.

By: J. Levin n, W. Thompson n, A. Csinos*, M. Stephenson* & A. Weissinger n

author keywords: gene silencing; matrix attachment regions; RNA-mediated virus resistance; Tomato Spotted Wilt Virus
MeSH headings : Breeding; DNA Methylation; Gene Expression Regulation; Gene Silencing; Immunity, Innate; Matrix Attachment Regions; Plants, Genetically Modified; Promoter Regions, Genetic; Tobacco / genetics; Tobacco / virology; Tospovirus / genetics; Tospovirus / pathogenicity; Transcription, Genetic
TL;DR: Results are consistent with the concept that MARs may prevent transcriptional silencing, and short interfering 21– 25 nt RNAs from the transgene that are characteristic of post-transcriptional gene silencing (PTGS) were present in the resistant R1 plants, but not the susceptible R4 plants, indicating that virus resistance was associated with PTGS of thetransgene. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

The rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells: A flow cytometric study

PLANT CELL, 17(2), 418–429.

By: C. Halweg n, W. Thompson n & S. Spiker n

MeSH headings : Agrobacterium tumefaciens / genetics; DNA, Bacterial / genetics; Flow Cytometry / methods; Gene Expression Regulation, Plant; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Matrix Attachment Regions; Plants, Genetically Modified; Promoter Regions, Genetic / genetics; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Tobacco / cytology; Tobacco / genetics; Transformation, Genetic; Transgenes / genetics
TL;DR: Flow cytometry is used to measure green fluorescent protein (GFP) expression in individual tobacco cells from lines transformed by Agrobacterium tumefaciens to conclude that MAR-mediated overall increases in transgene expression involve both likelihood and magnitude. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Establishment of rapidly proliferating rice cell suspension culture and its characterization by fluorescence-activated cell sorting analysis

PLANT MOLECULAR BIOLOGY REPORTER, 22(3), 259–267.

By: T. Lee n, R. Shultz n, L. Hanley-Bowdoin n & W. Thompson n

author keywords: cell suspension culture; FACS; nuclei isolation; Oryza sativa L.; pectinase
TL;DR: The studies described here demonstrate that NB2P culture can be a powerful tool for studying many important plant processes, including DNA replication and cell cycle-related pathways. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 chapter

Transgene Integration: Use of Matrix Attachment Regions

In Transgenic Plants (pp. 313–326).

By: G. Allen n, S. Spiker n & W. Thompson n

MeSH headings : Biolistics / methods; Cells, Cultured; Crops, Agricultural / genetics; Genetic Markers; Genetic Vectors; Nuclear Matrix / genetics; Plants, Genetically Modified / genetics; Protoplasts / physiology; Rhizobium / genetics; Tobacco / genetics; Tobacco / growth & development
TL;DR: This chapter describes methods for the isolation of MARs and the subsequent methods allowing the investigator to incorporate MARS into transformation strategies that can both improve transformation frequency and result in predictable, stable expression of the transgenic trait. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 24, 2019

2003 journal article

Analysis of trans-silencing interactions using transcriptional silencers of varying strength and targets with and without flanking nuclear matrix attachment regions

TRANSGENIC RESEARCH, 12(3), 305–318.

By: R. Ascenzi n, B. Ulker n, J. Todd n, D. Sowinski n, C. Schimeneck n, G. Allen n, A. Weissinger n, W. Thompson n

author keywords: matrix attachment region; tobacco; transcriptional gene silencing; transgene expression; trans-silencing
MeSH headings : Gene Silencing; Inheritance Patterns; Matrix Attachment Regions / genetics; Seedlings / genetics; Sequence Homology; Silencer Elements, Transcriptional; Tobacco / genetics; Transcription, Genetic
TL;DR: The results are consistent with a model in which transcriptional silencing is triggered by transcription of sequences during the early stages of embryo or seedling development. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3 ' MAR

TRANSGENIC RESEARCH, 12(1), 3–12.

By: S. Mankin n, G. Allen*, T. Phelan n, S. Spiker n & W. Thompson n

author keywords: gene silencing; matrix attachment regions; transformation; transgene expression
MeSH headings : 3' Untranslated Regions / genetics; DNA Primers / chemistry; Gene Expression Regulation, Plant; Genes, Plant / physiology; Glucuronidase / genetics; Glucuronidase / metabolism; Hot Temperature; Nuclear Matrix / physiology; Plant Leaves / genetics; Plant Leaves / growth & development; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Polymerase Chain Reaction; Promoter Regions, Genetic / genetics; Tobacco / genetics; Tobacco / growth & development; Transformation, Genetic; Transgenes / physiology; Up-Regulation
TL;DR: Most transgenes flanked by RB7 MAR elements showed a large reduction in the number of low expressing GUS transformants relative to control constructs without MARs, which is important for understanding the role of matrix attachment region in transgene expression. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Differential Top10 promoter regulation by six tetracycline analogues in plant cells

JOURNAL OF EXPERIMENTAL BOTANY, 53(376), 1871–1877.

By: J. Love*, G. Allen*, C. Gatz & W. Thompson*

author keywords: tetracycline analogues; tobacco; Top10 promoter activity; tissue culture
MeSH headings : Agrobacterium tumefaciens / genetics; Cell Line; Doxycycline / pharmacology; Gene Expression Regulation, Plant / drug effects; Glucuronidase / genetics; Glucuronidase / metabolism; Minocycline / pharmacology; Oxytetracycline / pharmacology; Plants, Genetically Modified; Promoter Regions, Genetic / genetics; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Repressor Proteins / genetics; Tetracyclines / chemistry; Tetracyclines / pharmacology; Tobacco / cytology; Tobacco / drug effects; Tobacco / genetics; Transfection
TL;DR: Different tetracycline analogues can therefore be used to regulate the Top10 promoter in plant cells and this property may be exploited in planning an optimum course of transgene regulation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

High-throughput transgene copy number estimation by competitive PCR

PLANT MOLECULAR BIOLOGY REPORTER, 20(3), 265–277.

By: A. Callaway n, R. Abranches n, J. Scroggs n, G. Allen n & W. Thompson n

author keywords: competitive; copy number; high throughput; PCR; quantitative; transgenic
TL;DR: This work applies mathematical modeling to create a rapid, simple, and inexpensive copy number determination method that retains the robustness of competitive PCR. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Up-regulation of phosphoinositide metabolism in tobacco cells constitutively expressing the human type I inositol polyphosphate 5-phosphatase

PLANT PHYSIOLOGY, 129(4), 1795–1806.

By: I. Perera n, J. Love n, I. Heilmann n, W. Thompson n & W. Boss n

MeSH headings : Cells, Cultured; Gene Expression Regulation, Enzymologic; Humans; Inositol 1,4,5-Trisphosphate / metabolism; Inositol Polyphosphate 5-Phosphatases; Intercellular Signaling Peptides and Proteins; Peptides / metabolism; Phosphatidylinositol 4,5-Diphosphate / metabolism; Phosphatidylinositols / metabolism; Phosphoric Monoester Hydrolases / genetics; Phosphoric Monoester Hydrolases / metabolism; Phosphorus Radioisotopes; Plants, Genetically Modified; Tobacco / cytology; Tobacco / genetics; Tobacco / metabolism; Up-Regulation; Wasp Venoms / chemistry; Wasp Venoms / metabolism
TL;DR: These studies show that the constitutive expression of the human type I InsP 5-ptase in tobacco cells leads to an up-regulation of the phosphoinositide pathway and highlight the importance of PtdInsP2 synthesis as a regulatory step in this system. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

When a day makes a difference. Interpreting data from endoplasmic reticulum-targeted green fluorescent protein fusions in cells grown in suspension culture

Plant Physiology, 128(2), 341–344.

By: S. Persson n, J. Love n, P. Tsou n, D. Robertson n, W. Thompson n & W. Boss n

MeSH headings : Calcium / metabolism; Calcium-Binding Proteins / genetics; Calcium-Binding Proteins / metabolism; Calreticulin; Cells, Cultured; Endoplasmic Reticulum / metabolism; Green Fluorescent Proteins; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Plasmids / genetics; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Ribonucleoproteins / genetics; Ribonucleoproteins / metabolism; Time Factors; Tobacco / cytology; Tobacco / genetics; Tobacco / metabolism
TL;DR: The stability of the self-contained structure of green fluorescent protein (GFP) has made it the most widely utilized fluorescent marker for gene expression and subcellular localization studies. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries, Crossref
Added: August 6, 2018

2001 journal article

Generation of Intron-Containing, ER-Localized, Soluble-Modified Green Fluorescent Protein Genes for use in Plant Transformation

Plant Molecular Biology Reporter, 19(1), 13–26.

By: S. Mankin n & W. Thompson n

TL;DR: Transient expression of theerGFPINTanderGFP genes is comparable in tobacco and maize suspension culture protoplasts, indicating that the PIV2 intron is spliced effectively in both monocotyledonous and dicotylingonous plant species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2001 patent

Method for reducing expression variability of transgenes in plant cells

Washington, DC: U.S. Patent and Trademark Office.

By: W. Thompson

Source: NC State University Libraries
Added: August 6, 2018

2001 journal article

The 5 ' end of the pea ferredoxin-1 mRNA mediates rapid and reversible light-directed changes in translation in tobacco

PLANT PHYSIOLOGY, 125(2), 770–778.

By: E. Hansen n, M. Petracek n, L. Dickey* & W. Thompson n

MeSH headings : 5' Untranslated Regions / genetics; Darkness; Ferredoxins / genetics; Light; Peas / genetics; Peas / radiation effects; Plant Leaves / genetics; Plant Leaves / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / radiation effects; Polyribosomes / genetics; Protein Biosynthesis / radiation effects; RNA, Messenger / genetics; Tobacco / genetics; Tobacco / radiation effects
TL;DR: Observations support a model in which Fed-1 mRNA in illuminated leaves is stabilized by its association with polyribosomes, and/or by translation, and that translation of other mRNAs is also rapidly down-regulated in response to darkness. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

The Ca2+ status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants

PLANT PHYSIOLOGY, 126(3), 1092–1104.

By: S. Persson n, S. Wyatt n, J. Love n, W. Thompson n, D. Robertson n & W. Boss n

MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Calcium / metabolism; Calcium-Binding Proteins / biosynthesis; Calreticulin; Cell Line; Endoplasmic Reticulum / metabolism; Heat-Shock Response; Phenotype; Plant Proteins / metabolism; Plants, Genetically Modified; Plants, Toxic; Ribonucleoproteins / biosynthesis; Tobacco / genetics; Tobacco / metabolism; Zea mays / genetics; Zea mays / metabolism
TL;DR: The level of CRT in heat shock-induced CRT transgenic plants correlated positively with the retention of chlorophyll when the plants were transferred from Ca( 2+)-containing medium to Ca(2+)-depleted medium, consistent with the hypothesis that increasingCRT in the ER increases the ERCa(2+) stores and thereby enhances the survival of plants grown in low Ca(1+) medium. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions

TRANSGENIC RESEARCH, 10(5), 465–470.

By: R. Ascenzi n, J. Ingram n, M. Massel n, W. Thompson n, S. Spiker n & A. Weissinger n

author keywords: cell differentiation and proliferation; chromatin; matrix; scaffold attachment region (MAR/SAR); plant high mobility group-I/Y protein (HMG-I/Y); transgene expression; tobacco
MeSH headings : Cell Differentiation; Cells, Cultured; Gene Expression; HMGA1a Protein / genetics; HMGA1a Protein / metabolism; Plants, Genetically Modified; Tobacco / cytology; Tobacco / genetics
TL;DR: It appears that tobacco nuclear matrix attachment region function is not enhanced simply because cells in culture divide rapidly and are not differentiated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Heat shock protein HSP101 binds to the Fed-1 internal light regulatory element and mediates its high translational activity

PLANT CELL, 12(7), 1213–1227.

By: J. Ling*, D. Wells*, R. Tanguay*, L. Dickey n, W. Thompson n & D. Gallie*

MeSH headings : Binding Sites; Ferredoxins / genetics; Ferredoxins / metabolism; Heat-Shock Proteins / metabolism; Light; Plants / metabolism; Protein Binding; Protein Biosynthesis; RNA-Binding Proteins / metabolism
TL;DR: The characterized RNA binding activities associated with the Fed-1 iLRE are identified as the heat shock protein HSP101, a protein shown to bind the 5′ leader of tobacco mosaic virus, and data suggest thatFed-1 mRNA may use the HSP 101 regulatory mechanism as a means of ensuring a high level of translation required for the light-mediated regulation of Fed- 1 mRNA stability. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 patent

Increasing expression of transgenes in plant cells using insulator elements

Washington, DC: U.S. Patent and Trademark Office.

By: W. Thompson, G. Allen & S. Mankin

Source: NC State University Libraries
Added: August 6, 2018

2000 patent

Method for reducing expression variability of transgenes in plant cells

Washington, DC: U.S. Patent and Trademark Office.

By: W. Thompson, G. Allen & S. Mankin

Source: NC State University Libraries
Added: August 6, 2018

2000 chapter

Post-Transcriptional Light Regulation of Nuclear-Encoded Genes

In Genetic Engineering (pp. 1–10).

By: M. Petracek* & W. Thompson n

MeSH headings : Cell Nucleus / genetics; Gene Expression Regulation, Plant; Light; Plants / genetics; RNA Processing, Post-Transcriptional
TL;DR: It is likely that with careful experimental design, reports of nuclear-encoded post-transcriptional gene regulation will become increasingly prevalent, because of possible changes in translation rates and protein turnover. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

2000 journal article

Premature termination codons destabilize ferredoxin-1 mRNA when ferredoxin-1 is translated

PLANT JOURNAL, 21(6), 563–569.

By: M. Petracek n, T. Nuygen n, W. Thompson n & L. Dickey*

MeSH headings : Cells, Cultured; Codon, Terminator; Ferredoxins / genetics; Mutation; Peas / genetics; Peas / metabolism; Protein Biosynthesis; RNA, Messenger / metabolism
TL;DR: It is shown that the nonsense codon effect results primarily from lowering the Fed-1 mRNA stability in light-treated leaf tissue and in rapidly growing tobacco cell cultures, but not in dark- treated leaf tissue, suggesting that nonsense codons trigger a decay pathway distinct from that seen for Fed- 1 mRNA in the dark. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Stringent control of transgene expression in Arabidopsis thaliana using the Top10 promoter system

PLANT JOURNAL, 21(6), 579–588.

By: J. Love*, A. Scott n & W. Thompson n

MeSH headings : Arabidopsis / genetics; Arabidopsis / virology; Gene Expression Regulation, Plant; Green Fluorescent Proteins; Luminescent Proteins / biosynthesis; Models, Genetic; Plants, Genetically Modified; Promoter Regions, Genetic; Recombinant Fusion Proteins / biosynthesis; Repressor Proteins / genetics; Simplexvirus / genetics; Tetracycline / pharmacology; Transgenes
TL;DR: GFP expression was repressed by 100 ng ml-1 tetracycline, an order of magnitude lower than the concentration used previously to repress expression in Nicotiana tabacum, allowing a titred regulation of transgenic activity that was previously unavailable in A. thaliana. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 review

Use of matrix attachment regions (MARs) to minimize transgene silencing

[Review of ]. PLANT MOLECULAR BIOLOGY, 43(2-3), 361–376.

By: G. Allen*, S. Spiker* & W. Thompson n

author keywords: chromatin structure; gene silencing; MAR; nuclear matrix; nuclear scaffold; SAR
MeSH headings : Animals; DNA / genetics; DNA / metabolism; Gene Expression Regulation, Plant; Gene Silencing; Humans; Nuclear Matrix / metabolism; Nuclear Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Protein Binding; Transgenes / genetics
TL;DR: This review explores current data and presents several plausible models to explain MAR effects on transgene expression and results in higher and more stable expression in transgenic plants or cell lines. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1999 journal article

A tobacco matrix attachment region reduces the loss of transgene expression in the progeny of transgenic tobacco plants

PLANT JOURNAL, 18(3), 253–263.

By: B. Ulker, G. Allen*, W. Thompson*, S. Spiker* & A. Weissinger*

TL;DR: In primary transgenic plants, the average uidA expression in plants with MARs was twofold greater than in control plants without MARs, but there was no effect on variation of expression. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1999 journal article

Characterization of randomly-obtained matrix attachment regions (MARs) from higher plants

BIOCHEMISTRY, 38(39), 12795–12804.

By: S. Michalowski n, G. Allen n, G. Hall n, W. Thompson n & S. Spiker n

MeSH headings : Base Sequence; Cloning, Molecular; DNA, Plant; Molecular Sequence Data; Nuclear Matrix / metabolism; Plants / metabolism
TL;DR: Binding strength is a function of both numbers of binding sites and affinity for the sites, and a newly identified motif, the "90%AT Box," correlates better with binding strength than any of the previously identified motifs investigated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1999 journal article

Matrix attachment regions increase transgene expression levels and stability in transgenic rice plants and their progeny

PLANT JOURNAL, 18(3), 233–242.

By: P. Vain*, B. Worland*, A. Kohli*, J. Snape*, P. Christou*, G. Allen n, W. Thompson n

Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1999 journal article

Transposon tagging of the sulfur gene of tobacco using engineered maize Ac/Ds elements

Genetics, 153(4), 1919–1928.

By: W. Fitzmaurice, L. Nguyen, E. Wernsman, W. Thompson & M. Conkling

Source: NC State University Libraries
Added: August 6, 2018

1998 journal article

Ferredoxin-1 mRNA is destabilized by changes in photosynthetic electron transport

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 95(15), 9009–9013.

By: M. Petracek n, L. Dickey n, T. Nguyen n, C. Gatz n, D. Sowinski n, G. Allen n, W. Thompson n

MeSH headings : Diuron / pharmacology; Electron Transport; Ferredoxins / genetics; Half-Life; Histones / genetics; Photosynthesis / drug effects; Plants, Genetically Modified / genetics; Plants, Toxic; Promoter Regions, Genetic; RNA, Messenger / metabolism; Tetracycline / pharmacology; Tobacco / genetics
TL;DR: The tetracycline repressible Top10 promoter system is used to specifically shut off transcription of the Fed-1 transgene, and data indicate that cessation of photosynthesis, either by darkness or DCMU results in a destabilization of theFed-1 mRNA. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1998 journal article

Gene silencing from plant DNA carried by a Geminivirus

PLANT JOURNAL, 14(1), 91–100.

By: S. Kjemtrup n, K. Sampson n, C. Peele n, L. Nguyen n, M. Conkling n, W. Thompson n, D. Robertson n

TL;DR: The results establish that homologous DNA sequences localized in nuclear episomes can modulate the expression of active chromosomal genes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1998 journal article

Individual members of the light-harvesting complex II chlorophyll a/b binding protein gene family in pea (Pisum sativum) show differential responses to ultraviolet-B radiation

Physiologia Plantarum, 103(3), 377–384.

By: S. MacKerness, L. Liu, B. Thomas, W. Thompson*, B. Jordan & M. White

TL;DR: The stage during de-etiolation at which supplementary UV-B began to inhibit Lhcb gene expression was determined, suggesting that the developmental stage of chloroplasts may be important in determining the responses of the LhCB genes to supplementaryUV-B radiation in pea seedlings. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1998 journal article

Light regulation of Fed-1 mRNA requires an element in the 5' untranslated region and correlates with differential polyribosome association

Plant Cell, 10(3), 475–484.

By: L. Dickey*, M. Petracek, T. Nguyen*, E. Hansen* & W. Thompson*

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1998 patent

Method of increasing expression for foreign genes in plant cells

Washington, DC: U.S. Patent and Trademark Office.

George Allen; Steven Spiker; William Thompson

Source: NC State University Libraries
Added: August 6, 2018

1998 patent

Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells

Washington, DC: U.S. Patent and Trademark Office.

William Thompson; George Allen; Steven Spiker

Source: NC State University Libraries
Added: August 6, 2018

1998 chapter

The dependence of Fed-1 light regulation on translation

In J. Bailey-Serres & D. R. Gallie (Eds.), A look beyond transcription: Mechanisms determining mRNAstability and translation in plants (pp. 96–101). Washington, DC: American Society of Plant Physiologists.

By: M. Petracek, L. Dickey, E. Hansen, D. Sowinski, T. Nguyen, G. Allen, G. Thompson

Ed(s): . J. Bailey-Serres & D. Gallie

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Characterization of post transcriptionally suppressed transgene expression that confers resistance to tobacco etch virus infection in tobacco

Plant Cell, 9(8), 1411–1423.

By: M. Tanzer n, W. Thompson n, M. Law n, E. Wernsman n & S. Uknes n

TL;DR: It is shown that at early times in development, immune lines are susceptible to TEV infection and accumulate full-length CP mRNA, and a hypothesis for transgene/viral RNA degradation in which RNA degradation occurs in the cytoplasm while in association with polyribosomes is presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1997 journal article

Introduction of a plant intron into the luciferase gene of Photinus pyralis

Plant Molecular Biology Reporter, 15(2), 186–196.

By: S. Mankin n, G. Allen n & W. Thompson n

TL;DR: A new luciferase reporter gene,lucINT, is described, allowing earlier detection of gene expression in the presence of Agrobacterium during transformations in tobacco leaf discs. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1997 journal article

Light-regulated changes in abundance and polyribosome association of ferredoxin mRNA are dependent on photosynthesis

Plant Cell, 9(12), 2291–2300.

By: M. Petracek, L. Dickey*, S. Huber* & W. Thompson*

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1997 journal article

Sites of rDNA transcription are widely

Plant Journal, 12(1997), 571–581.

By: W. Thompson, A. Bevan, B. Wells & P. Shaw

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

The dark-adaptation response of the de-etiolated pea mutant lip1 is modulated by external signals and endogenous programs

PLANT PHYSIOLOGY, 115(1), 23–28.

By: S. Frances n & W. Thompson n

MeSH headings : Adaptation, Physiological; Darkness; Genes, Plant; Light; Mutation; Peas / genetics; Peas / physiology; Peas / radiation effects; Phenotype; Plant Proteins / genetics
TL;DR: It is concluded that environmental signals (light) and endogenous programs (developmental and circadian) regulate gene expression in both WT and mutant plants, however, mutant seedlings exhibit a developmentally regulated and exaggerated response to light. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1996 journal article

High-Level Transgene Expression in Plant Cells: Effects of a Strong Scaffold Attachment Region from Tobacco

The Plant Cell, 8(5), 899.

By: G. Allen*, G. Hall, S. Michalowski, W. Newman, S. Spiker, A. Weissinger, W. Thompson*

Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1996 chapter

Matrix Attachment Regions and Transgene Expression

In Genomes of Plants and Animals (pp. 243–269).

By: W. Thompson n, G. Allen n, G. Hall n & S. Spiker n

TL;DR: Many of the questions the authors have about how biological systems work are ultimately questions about the regulation of gene expression, and the control of transcription is fundamental and has received well-deserved attention. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1996 journal article

Nuclear Matrix Attachment Regions and Transgene Expression in Plants

Plant Physiology, 110(1), 15–21.

By: S. Spiker n & W. Thompson*

TL;DR: The hypothesized role of MARs in chromatin structure, how MARs are isolated and characterized, what effects MARs have had on the expression of transgenes and the models that have been evoked to explain those effects are discussed. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

1996 chapter

Post-transcriptional regulation by light

In W. R. Briggs, R. L. Heath, & E. M. Tobin (Eds.), Regulation of Plant Growth and Development by Light. Rockville, MD: American Society of Plant Physiologists.

By: M. Petracek, L. Dickey, T. Nguyen, G. Allen, D. Sowinski, E. Hansen, W. Thompson

Ed(s): W. Briggs, R. Heath & E. Tobin

Source: NC State University Libraries
Added: April 11, 2019

1995 conference paper

A nuclear scaffold attachment region from tobacco greatly increases transgene expression in plant cells

In R. Phillips & K. Oono (Eds.), Proceedings of the US-Japan Symposium on Modification of Gene Expression and Non-Mendelian Inheritance (pp. 281–295).

By: W. Thompson, G. Allen, G. Hall, S. Michalowski, W. Newman, S. Spiker, A. Weissinger

Ed(s): R. Phillips & K. Oono

Source: NC State University Libraries
Added: April 11, 2019

1995 journal article

Individual Members of the Cab Gene Family Differ Widely in Fluence Response

Plant Physiology, 107(1), 161–165.

By: M. White*, L. Kaufman*, B. Horwitz*, W. Briggs* & W. Thompson*

TL;DR: Very low fluence responses and high irradiance responses occur in the same gene family of chlorophyll a/b-binding protein genes. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1995 journal article

Light-regulated expression of the Arabidopsis thaliana ferredoxin gene requires sequences upstream and downstream of the transcription initiation site

Plant Molecular Biology, 27(1), 27–39.

By: A. Bovy*, C. Van Den Berg*, G. De Vrieze*, W. Thompson n, P. Weisbeek* & S. Smeekens*

author keywords: ARABIDOPSIS THALIANA; CHIMERIC GENES; FERREDOXIN; LIGHT REGULATION; NICOTIANA TABACUM
MeSH headings : Arabidopsis / genetics; Arabidopsis / radiation effects; Base Sequence; DNA, Plant / genetics; DNA, Recombinant / genetics; Ferredoxins / genetics; Gene Expression Regulation, Plant; Genes, Plant / genetics; Light; Molecular Sequence Data; Peas / genetics; Plants, Genetically Modified; Plants, Toxic; Promoter Regions, Genetic / genetics; RNA, Messenger / analysis; Regulatory Sequences, Nucleic Acid / genetics; Tobacco / genetics; Transcription, Genetic / genetics
TL;DR: Test of chimeric genes containing various parts of the fedA gene for light-dependent expression in mature tobacco plants suggest that for full light-regulated expression of thefedA gene, both the promoter region and sequences downstream of the transcription start site are required. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1994 journal article

Light modulation of ferredoxin mRNA abundance requires an open reading frame

Plant Cell, 6, 1171–1176.

By: L. Dickey, F. Nguyen, G. Allen & W. Thompson*

UN Sustainable Development Goal Categories
4. Quality Education (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: April 21, 2019

1994 journal article

Spotlight on phytochrome nomenclature

Plant Cell, 6, 468–471.

By: P. Quail, W. Briggs, J. Chory, R. Hangarter, N. Harberd, R. Kendrick, M. Koorneef, B. Parks ...

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 21, 2019

1993 chapter

Ferredoxin gene expression and its regulation by light

In D. P. S. Verma (Ed.), Control of Plant Gene Expression (pp. 211–222). Boca Raton, FL: CRC Press.

By: L. Dickey, M. Gallo-Meagher & W. Thompson

Ed(s): D. Verma

Source: NC State University Libraries
Added: April 11, 2019

1993 journal article

Scaffold attachment regions increase reporter gene expresssion in stably transformed plant cells

Plant Cell, 5(6), 603–613.

By: G. Allen*, G. Hall, L. Childs, A. Weissinger, S. Spiker & W. Thompson*

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1993 journal article

Sequence of Lhcb3∗1, a gene encoding a Photosystem II chlorophyll protein in Pisum

Biochimica Et Biophysica Acta (BBA) - Gene Structure and Expression, 1173(3), 333–336.

By: D. Falconet*, C. Godon*, M. White n & W. Thompson n

author keywords: LHCB GENE; CHLOROPHYLL A/B-BINDING PROTEIN; NUCLEOTIDE SEQUENCE; (PISUM)
MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; Fabaceae; Genes, Plant; Light-Harvesting Protein Complexes; Molecular Sequence Data; Photosynthetic Reaction Center Complex Proteins / chemistry; Photosynthetic Reaction Center Complex Proteins / genetics; Photosystem II Protein Complex; Plants, Medicinal
TL;DR: Cloned and sequenced a pea Lhcb3 gene, encoding a Photosystem II chlorophyll a/b-binding protein, indicates that the gene contains two introns and predicts a polypeptide of 265 amino acids, which is highly homologous to the polypePTide sequences deduced from LhCB3 genes previously characterized in tomato and barley. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1992 journal article

Both internal and external elements control expression of the pea Fed-1 gene in etiolated seedlings

Plant Cell, 4, 389–395.

By: M. Gallo-Meagher, D. Sowinski, R. Elliott & W. Thompson*

Sources: NC State University Libraries, NC State University Libraries
Added: April 21, 2019

1992 journal article

Development and characterization of a generalized gene tagging system for higher plants using an engineered maize transposon Ac

Plant Molecular Biology, 20(2), 177–198.

By: W. Fitzmaurice n, L. Lehman n, L. Nguyen n, W. Thompson n, E. Wernsman n & M. Conkling n

author keywords: AC; DS; BETA-GLUCURONIDASE; METHOTREXATE RESISTANCE; TOBACCO; TRANSPOSON TAGGING
MeSH headings : Base Sequence; Blotting, Southern; Cloning, Molecular / methods; Crosses, Genetic; DNA; DNA Transposable Elements; Drug Resistance / genetics; Genetic Engineering; Genetic Markers; Glucuronidase / genetics; Methotrexate / pharmacology; Molecular Sequence Data; Nucleotidyltransferases / genetics; Nucleotidyltransferases / metabolism; Plants / genetics; Plants, Toxic; Polymerase Chain Reaction; Promoter Regions, Genetic; Rhizobium; Seeds / genetics; Tobacco; Transformation, Genetic; Transposases; Zea mays / genetics
TL;DR: This binary system includes the transposase (Ts) and the tagging element (Ds) on separate T-DNA vectors for dicotyledonous plants and can generate a sufficient number of events to be useful in gene tagging. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

1992 journal article

Expression of the chlorophyll-a/b-protein multigene family in pea (Pisum sativum L.)

Planta, 188(2), 190–198.

By: M. White n, B. Fristensky*, D. Falconet*, L. Childs n, J. Watson*, L. Alexander*, B. Roe*, W. Thompson n

author keywords: CHLOROPHYLL A/B PROTEIN; GENE EXPRESSION (CAB); LIGHT AND GENE EXPRESSION; LIGHT-HARVESTING
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

1992 chapter

Ferredoxin gene expression and its regulation by light

In D. P. S. Verma (Ed.), Control of Gene Expression (pp. 211–222). CRC Press.

By: L. Dickey, M. Gallo-Meagher & W. Thompson

Ed(s): D. Verma

Source: NC State University Libraries
Added: April 21, 2019

1992 journal article

Initial characterization of a pea mutant with light-independent photomorphogenesis

Plant Cell, 4(12), 1519–1530.

By: S. Frances, M. White, M. Edgerton, A. Jones, R. Elliott & W. Thompson*

Sources: NC State University Libraries, NC State University Libraries
Added: June 8, 2019

1992 journal article

Light regulatory sequences are located within the 5' portion of the Fed-1 message sequence

EMBO Journal, 11(6), 2311–2317.

By: L. Dickey n, M. Gallo-Meagher n & W. Thompson n

author keywords: CHIMERIC GENES; CIS-ACTING ELEMENTS; FERREDOXIN; LIGHT REGULATION; NICOTIANA-TABACUM; PISUM-SATIVUM
MeSH headings : Chromosome Deletion; Cloning, Molecular; Genes; Light; Plants, Genetically Modified; Plants, Toxic; Polymerase Chain Reaction; Protein Sorting Signals / genetics; RNA, Messenger / genetics; RNA, Messenger / metabolism; Regulatory Sequences, Nucleic Acid; Tobacco / genetics; Tobacco / radiation effects; Transcription, Genetic
TL;DR: It is reported here that cis‐acting elements capable of mediating a 5‐fold light‐induced increase in the abundance of this mRNA are located within a region comprising the 5′ leader and first third of the Fed‐1 coding sequence. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1992 journal article

The pea ferredoxin I gene exhibits different light responses in etiolated seedlings of pea and tobacco

Plant Cell, 4, 383–388.

By: M. Gallo-Meagher, D. Sowinski & W. Thompson*

Sources: NC State University Libraries, NC State University Libraries
Added: April 21, 2019

1991 journal article

Concatemer chain reaction: A taq DNA polymerase-mediated mechanism for generating long tandemly repetitive DNA sequences

Analytical Biochemistry, 199(2), 184–190.

By: M. White n, B. Fristensky n & W. Thompson n

MeSH headings : Base Sequence; DNA / metabolism; DNA, Single-Stranded / biosynthesis; DNA-Directed DNA Polymerase / metabolism; Dideoxynucleosides / metabolism; Gene Amplification; Molecular Sequence Data; Nucleic Acid Conformation; Oligonucleotides; Repetitive Sequences, Nucleic Acid; Taq Polymerase
TL;DR: The concatemer chain reaction (CCR) uses Taq DNA polymerase to synthesize double- or single-stranded DNA concatemers whose length and yield can be controlled by varying the number of thermal cycling steps. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1991 journal article

High mobility group chromosomal proteins bind to AT-rich tracts flanking plant genes

Plant Molecular Biology, 16(1), 95–104.

By: T. Pedersen n, L. Arwood n, S. Spiker n, M. Guiltinan* & W. Thompson n

author keywords: AT-RICH SEQUENCES; DNA-BINDING PROTEINS; FERREDOXIN-I; HMG; HIGH MOBILITY GROUP PROTEINS
MeSH headings : Adenine; Base Composition; Base Sequence; DNA / chemistry; DNA / metabolism; Ferredoxins / genetics; High Mobility Group Proteins / metabolism; Molecular Sequence Data; Oligodeoxyribonucleotides / metabolism; Oligonucleotide Probes / metabolism; Plants / genetics; Poly dA-dT / metabolism; Thymine
TL;DR: HMG binding to the Fed-1 DNA was localized to a region containing AT-rich sequences; very similar sequences are present 5′ to Em and several other plants genes, suggesting a role for HMG binding which is consistent with previous evidence linking HMGs with transcriptionally competent chromatin. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

1991 journal article

Identification of the mitochondrial genome in the chrysophyte alga Ochromonas danica

Journal of Protozoology, 38(2), 129–135.

By: A. Coleman*, W. Thompson n & L. Goff

author keywords: DNA; OCHROMONAS-MINUTA; PLASTID DNA; POTERIOCHROMONAS-MALHAMENSIS; RDNA; SPECIATION
TL;DR: Analysis of total DNA isolated from the Chrysophyte alga Ochromonas danica revealed, in addition to nuclear DNA, two genomes present as numerous copies per cell, which suggest that all classes of eukaryote algae may have mitochondrial genomes < 100 kbp in size, more like other protistans than land plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1991 journal article

Nuclear scaffold and scaffold attachment regions (SARs) in higher plants

Proceedings of the National Academy of Sciences of United States of America, 88(20), 9320–9324.

By: G. Hall n, G. Allen n, D. Loer n, W. Thompson n & S. Spiker n

author keywords: CHROMATIN; CHROMOSOMES; NUCLEI; TOBACCO
TL;DR: This work has used Southern hybridization to show that the DNA remaining bound to the scaffolds after nuclease digestion includes SARs flanking three root-specific tobacco genes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1991 journal article

Nucleotide sequence ofCab-215, a Type II gene encoding a photosystem II chlorophylla/b-binding protein inPisum

Plant Molecular Biology, 17(1), 135–139.

By: D. Falconet n, M. White n, B. Fristensky n, M. Dobres n & W. Thompson n

author keywords: CAB GENES; CHLOROPHYLL A/B-BINDING PROTEIN; PISUM-SATIVUM; NUCLEOTIDE SEQUENCE
MeSH headings : Amino Acid Sequence; Base Sequence; Chlorophyll / metabolism; Chlorophyll A; DNA; Fabaceae / genetics; Light-Harvesting Protein Complexes; Molecular Sequence Data; Photosynthetic Reaction Center Complex Proteins / genetics; Photosystem II Protein Complex; Plants, Medicinal
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

1991 journal article

Nucleotide sequence ofCab-8, a new type I gene encoding a chlorophylla/b-binding protein of LHC II inPisum

Plant Molecular Biology, 17(3), 523–526.

By: L. Alexander n, D. Falconet n, B. Fristensky n, M. White n, J. Watson*, B. Roe*, W. Thompson n

author keywords: CAB GENES; CHLOROPHYLL-A/B-BINDING PROTEIN; PISUM-SATIVUM; NUCLEOTIDE SEQUENCE
MeSH headings : Amino Acid Sequence; Base Sequence; Chlorophyll / metabolism; Chlorophyll A; Consensus Sequence; DNA; Fabaceae / genetics; Light-Harvesting Protein Complexes; Molecular Sequence Data; Photosynthetic Reaction Center Complex Proteins / genetics; Photosynthetic Reaction Center Complex Proteins / metabolism; Plants, Medicinal
TL;DR: This work aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about the phytochemical properties of E.coli found in fruits and vegetables cultivated in North Carolina. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

1991 review

PHYSIOLOGICAL AND MOLECULAR STUDIES OF LIGHT-REGULATED NUCLEAR GENES IN HIGHER-PLANTS

[Review of ]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 42, 423–466.

By: W. Thompson* & M. White

author keywords: GENE EXPRESSION; PHOTOBIOLOGY; TRANSCRIPTION; POSTTRANSCRIPTIONAL CONTROL; TRANSLATIONAL CONTROL; POSTTRANSLATIONAL CONTROL
TL;DR: Comparing Transcription and mRNA Abundance and In Vitro Experiments, and the Ferredoxin System, are compared. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1991 journal article

The Inheritance and Linkage Mapping of Ferredoxin-1 in Pea

Journal of Heredity, 82(3), 259–261.

By: N. Polans, K. Folta, R. Elliott & W. Thompson*

Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1991 chapter

Unusual Features of the Light Response System Regulating Ferredoxin Gene Expression

In Phytochrome Properties and Biological Action (pp. 201–216).

By: W. Thompson n, R. Elliott n, L. Dickey n, M. Gallo n, T. Pedersen n & D. Sowinski n

TL;DR: The light responses of this gene — which is designated Fed-1 — differ strikingly from those of RbcS and Cab which have so far served as the main models for light responsive genes, providing an opportunity to significantly broaden the understanding of light regulatory mechanisms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1990 journal article

Light regulated expression of the Fed-1 gene in pea involves cis-acting elements within the transcription unit

Monograph (British Society for Plant Growth Regulation), (20), 3.

By: W. Thompson, R. Elliott, L. Dickey, B. Fristensky & M. White

Source: NC State University Libraries
Added: August 6, 2018

1990 chapter

Light regulated expression of the Fed-1 gene in pea involves cis-acting elements within the transcription unit

In T. Thomas & A. Smith (Eds.), British Society for Plant Growth Regulation, Monograph 20, Mechanisms of Plant Perception and Response to Environmental Stimuli (pp. 3–17).

By: W. Thompson, R. Elliott, L. Dickey, B. Fristensky & M. White

Ed(s): T. Thomas & A. Smith

Source: NC State University Libraries
Added: April 11, 2019

1989 journal article

A developmentally regulated bud specific mRNA sequence in pea has sequence similarity to seed lectins

Plant Physiology, 89(3), 833–838.

By: M. Dobres* & W. Thompson*

TL;DR: A transcript to a previously isolated cDNA clone, pEA207 is identified which accumulates in the actively growing bud of the pea plant and is either absent or present at very low levels in the expanded leaves below the bud. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1989 journal article

Characterization of a single copy gene encoding ferredoxin I from pea

The Plant Cell, 1, 681–690.

By: R. Elliott, T. Pedersen, B. Fristensky, M. White, L. Dickey & W. Thompson

Source: NC State University Libraries
Added: April 11, 2019

1989 journal article

Chloroplast gene expression in lettuce grown under different irradiances

Planta, 178(1), 69–75.

By: B. Jordan*, J. Hopley* & W. Thompson n

TL;DR: Three days after transfer from low-light into high-light, increased transcript levels were found for atp BE, although no change was detected for the psa A or psb A transcripts, and with increasing leaf age the RNA per chlorophyll becomes similar for both light conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1989 journal article

Cis-acting elements for light regulation of pea ferredoxin I gene expression are located within transcribed sequences

The Plant Cell, 1, 691–698.

By: R. Elliott, L. Dickey, M. White & W. Thompson

Source: NC State University Libraries
Added: April 11, 2019

1989 journal article

The identification and localization of 33 pea chloroplast transcription initiation sites

Current Genetics, 16(5-6), 433–445.

By: N. Woodbury*, M. Dobres* & W. Thompson*

MeSH headings : Base Sequence; Blotting, Northern; Chloroplasts / metabolism; DNA / genetics; Fabaceae / genetics; Genes, Plant; Guanosine Triphosphate / metabolism; Immunoblotting; Molecular Sequence Data; Multigene Family; Nucleotidyltransferases / metabolism; Plants, Medicinal; RNA / genetics; RNA Caps; Restriction Mapping; Ribonucleases / metabolism; Transcription, Genetic
TL;DR: A novel approach to produce a comprehensive transcription initiation map of the pea chloroplast genome by measuring the ability of DNA probes to protect 5′ ends of transcripts that have been capped in vitro. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1988 journal article

A transcription map of the pea chloroplast genome

Current Genetics, 14(1), 75–89.

By: N. Woodbury*, L. Roberts*, J. Palmer* & W. Thompson*

TL;DR: This study indicates that chloroplast genes are often grouped into multigene transcriptional units which can be cotranscribed, and that light-stimulated plastid development involves changes in the relative abundance of the overlapping RNAs of different length that result from transcription of these genes or gene clusters. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1988 chapter

Chromatin structure and expression of plant ribosomal RNA genes

In G. Kahl (Ed.), Architecture of Eukaryotic Genes (pp. 385–396). Weinheim: VCH Verlagsgesellschaft.

By: W. Thompson, R. Flavell, J. Watson & L. Kaufman

Ed(s): G. Kahl

Source: NC State University Libraries
Added: April 11, 2019

1988 journal article

Cytosine methylation in ribosomal DNA and nucleolus organiser expression in wheat

Journal of Molecular Biology, 204, 500–534.

By: R. Flavell, M. O'Dell & W. Thompson*

MeSH headings : Binding Sites; Cytosine / metabolism; Gene Expression Regulation; Genes; Methylation; Nucleolus Organizer Region; RNA, Ribosomal / metabolism; Repetitive Sequences, Nucleic Acid; Restriction Mapping; Triticum
TL;DR: It is concluded that cytosine methylation in rDNA is regulated and that the methylation pattern correlates with the transcription potential of an rRNA gene. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1988 journal article

DNase I sensitivity of ribosomal RNA Genes in chromatin and nucleolar dominance in wheat

Journal of Molecular Biology, 204(3), 535–548.

By: W. Thompson* & R. Flavell

MeSH headings : Binding Sites; Chromatin / genetics; Deoxyribonuclease I / genetics; Gene Expression Regulation; Genes; Methylation; Nucleolus Organizer Region; RNA, Ribosomal / genetics; Restriction Mapping; Triticum
TL;DR: It is shown that rRNA genes at dominant loci are organized in a chromatin conformation that renders them more sensitive to DNase I digestion than other r RNA genes. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1988 journal article

Inversions in legume chloroplast DNAs

Current Genetics, 14, 65–74.

By: J. Palmer, B. Osorio & W. Thompson

Source: NC State University Libraries
Added: April 11, 2019

1988 journal article

Light Effects on Several Chloroplast Components in Norflurazon-Treated Pea Seedlings

PLANT PHYSIOLOGY, 88(2), 340–347.

By: A. Sagar*, B. Horwitz, R. Elliott, W. Thompson* & W. Briggs

TL;DR: Results suggest that accumulation of the protein and its mRNA are coordinated with chlorophyll abundance at several steps, and post-transcriptional regulation at the level of mRNA and protein stability, there may exist coordination at the transcriptional stage. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1988 book

Molecular Plant Development

Englewood Cliffs, NJ: Prentice Hall.

By: T. Murphy & W. Thompson

Source: NC State University Libraries
Added: April 11, 2019

1988 journal article

Nuclear-Cytoplasmic Partitioning of Phytochrome-Regulated Transcripts in Pisum sativum

PLANT PHYSIOLOGY, 88(4), 1397–1402.

By: A. Sagar, W. Briggs & W. Thompson*

Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1988 chapter

Patterns of phytochrome-induced gene expression in etiolated pea buds

In Biomechanisms Regulating Growth and Development (pp. 269–284).

By: W. Thompson n, L. Kaufman*, B. Horwitz*, A. Sagar*, J. Watson* & W. Briggs*

TL;DR: The response diversity observed is evidence that several different signal transduction pathways are operative in regulating the levels of different mRNAs and suggests that the molecular mechanisms by which light affects gene expression differ for different genes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1988 journal article

Photoregulation: diverse gene responses in greening seedlings

Plant, Cell and Environment, 11(5), 319–328.

By: W. Thompson n

TL;DR: Light effects on the expression of nuclear genes for plastid proteins and for the 18S, 5.8S and 25S ribosomal RNAs are discussed and evidence for light effects at both transcriptional and post-transcriptional levels is given. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1988 journal article

Phytochrome Regulation of Greening in Pisum: Chlorophyll Accumulation and Abundance of mRNA for the Light-Harvesting Chlorophyll a/b Binding Proteins

PLANT PHYSIOLOGY, 86(1), 299–305.

By: B. Horwitz*, W. Thompson* & W. Briggs

TL;DR: The level of Cab mRNA, as measured by the presence of sequences hybridizing to a cDNA probe, does not limit the rate of chlorophyll accumulation after transfer of pea seedlings to white light, and the Cab mRNA level in the buds of seedlings grown under continuous red light remained high even when the red fluence rate was too low to allow significant greening. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1988 chapter

Purification and restriction endonuclease analysis of plant nuclear DNA

In A. Weissbach & H. Weissbach (Eds.), Methods for Plant Molecular Biology. Orlando FL: Academic Press.

By: J. Watson & W. Thompson

Ed(s): A. Weissbach & H. Weissbach

Source: NC State University Libraries
Added: April 11, 2019

1987 journal article

A phytochrome-regulated transcript encodes ferredoxin in Pisum sativum

Plant Molecular Biology, 8, 53–59.

By: M. Dobres*, R. Elliot*, J. Watson* & W. Thompson*

TL;DR: Genomic blotting analysis indicates that ferredoxin I is encoded by one or two genes, and the deduced amino acid sequence includes part of an hydrophobic transit sequence that shows only very limited homology to that of Silene pratensis ferred toxin. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1987 journal article

Chloroplast DNA evolution among legumes: Loss of a large inverted repeat occurred prior to other sequence rearrangements

Current Genetics, 11(4), 275–286.

By: J. Palmer*, B. Osorio*, J. Aldrich & W. Thompson*

TL;DR: It is concluded that the inverted repeat has been lost only once among legumes and that this loss occurred prior to all the other rearrangements observed in subclover, broad bean and pea. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1987 journal article

Developmental regulation of cytosine methylation in the nuclear ribosomal RNA genes of Pisum sativum

Journal of Molecular Biology, 193(1), 15–26.

By: J. Watson*, L. Kaufman* & W. Thompson*

MeSH headings : Cell Nucleus; Cytosine; DNA Restriction Enzymes; DNA, Ribosomal; Gene Expression Regulation; Genes; Methylation; Nucleic Acid Hybridization; Plant Development; Plants / genetics; RNA, Ribosomal / genetics
TL;DR: Data indicate that cytosine methylation levels may be related to the regulated expression of the nuclear rRNA genes in pea, and DNA obtained from the apical buds of pea seedlings is highly methylated. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1987 journal article

Light-regulated changes in DNase I hypersensitive sites in the rRNA genes of Pisum sativum

Proceedings of the National Academy of Sciences, 84(6), 1550–1554.

By: L. Kaufman n, J. Watson n & W. Thompson n

TL;DR: The rDNA chromatin of Pisum sativum plants grown with or without exposure to light is examined for the presence of DNase I hypersensitive sites and possible developmental changes in their distribution to find that several sites exist in both the coding and noncoding regions of rDNA repeating units. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1987 journal article

Specific mRNA and rRNA Levels in Greening Pea Leaves during Recovery from Iron Stress

PLANT PHYSIOLOGY, 84(2), 409–414.

By: S. Spiller*, L. Kaufman, W. Thompson* & W. Briggs

TL;DR: Kinetic considerations suggest that the changes observed represent a coordinate series of events initiated by readdition of Fe and occurring in parallel, and that a rhythm in greening leaves was hard to detect and was prominent in the Fe-sufficient controls, persisting undamped through three full cycles for both mRNAs and hence is probably circadian. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1987 journal article

Structure and variation in ribosomal RNA genes of pea: Characterization of a cloned rDNA repeat and chromosomal rDNA variants

Plant Molecular Biology, 8(1), 3–12.

By: R. Jorgensen*, R. Cuellar* & W. Thompson*

TL;DR: Comparison of rDNA restriction maps for two pea genotypes separated for hundreds or perhaps thousands of generations reveals that they contain many rDNA identical repeat units, consistent with the view that new rDNA variants are fixed only infrequently in the evolution of a species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1986 journal article

Distribution, inheritance and linkage relationships of ribosomal DNA spacer length variants in pea

Theoretical and Applied Genetics, 72(3), 289–295.

By: N. Polans*, N. Weeden* & W. Thompson*

TL;DR: It is suggested that Rrn2 and the isozyme loci linked to it constitute a new linkage group on chromosome 7, which indicates that little or no genetic exchange occurs between the nonhomologous nucleolar organizer regions. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1986 journal article

Phytochrome Control of Specific mRNA levels in Developing Pea Buds : Kinetics of Accumulation, Reciprocity, and Escape Kinetics of the Low Fluence Response

PLANT PHYSIOLOGY, 81(4), 1033–1038.

By: L. Kaufman*, L. Roberts*, W. Briggs* & W. Thompson*

TL;DR: A comparison of the time course and escape kinetic data indicates that relatively rapid turnover of the RNA must occur in pea buds after irradiating dark grown seedlings with a single pulse low fluence red light. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1986 journal article

Purification and restriction endonuclease analysis of plant nuclear DNA

Methods in Enzymology, 118, 57–75.

By: J. Watson & W. Thompson*

TL;DR: The rationale of the methods for the isolation of DNA from purified nuclei have the features, such as a pretreatment of the tissue to enhance cell disruption, homogenization in the presence of membrane stabilizing agents, filtration to remove whole cells and large debris, and purification of the nuclei by density gradient centrifugation. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: June 8, 2019

1986 journal article

Structural evolution and flip-flop recombination of chloroplast DNA in the fern genus Osmunda

Current Genetics, 10(11), 835–841.

By: D. Stein*, J. Palmer* & W. Thompson*

TL;DR: Each of the three fern chloroplast genomes exists as an equimolar population of two isomeric circles differing only in the relative orientation of their two single copy regions, inferred to result from high frequency intramolecular recombination between paired inverted repeat segments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1986 journal article

The differential expression of ribosomal RNA genes

Philosophical Transactions of Royal Society of London, B314(1166), 385–397.

By: R. Flavell, M. O'Dell, W. Thompson*, M. Vincentz, R. Sardana & R. Barker

TL;DR: From comparison of this gene structure with that of Xenopus ribosomal RNA genes it can be concluded that these short sequences are likely to act as enhancers of transcription by binding to specific regulatory proteins that function to stimulate the attachment of polymerase I complexes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1986 journal article

The watermelon mitochondrial URF-1 gene: evidence for a complex structure

Current Genetics, 10(11), 857–869.

By: D. Stern*, A. Bang* & W. Thompson*

MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; Codon / genetics; DNA, Mitochondrial / genetics; Exons; Genes; Introns; Molecular Sequence Data; Plant Proteins / genetics; Plants / genetics; Transcription, Genetic
TL;DR: This is the first description of a plant mitochondrial gene with multiple introns, and it is postulate that three additional exons, selected by their map location and amino acid homology to other URF-1 sequences, encode the remainder of the polypeptide. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1985 journal article

Chloroplast DNA variation and evolution in Pisum: patterns of change and phylogenetic analysis

Genetics, 109, 195–213.

By: J. Palmer, R. Jorgensen & W. Thompson

Source: NC State University Libraries
Added: April 11, 2019

1985 chapter

Chromosome architecture: the distribution of recombination sites, the structure of ribosomal DNA loci, and the multiplicity of sequences containing inverted repeats

In L. van Vloten-Doting, G. S. P. Groot, & T. C. Hall (Eds.), Molecular Form and Function of the Plant Genome. New York: Plenum Press.

By: R. Flavell, M. O'Dell, D. Smith & W. Thompson

Ed(s): L. van Vloten-Doting, G. Groot & T. Hall

Source: NC State University Libraries
Added: June 8, 2019

1985 journal article

Induction of plant gene expression by light

BioEssays, 3(4), 153–159.

By: W. Thompson*, L. Kaufman* & J. Watson*

TL;DR: In vitro mutagenesis and gene transfer experiments with the gene for the small subunit of RuBP carboxylase indicate that sequences involved in regulating the level of expression in the light are located 5′ to the gene within about 1 kb of the start of transcription. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1985 journal article

Inheritance, organization, and mapping of rbcS and cab multigene families in pea

Proceedings of the National Academy of Sciences, 82(15), 5083–5087.

By: N. Polans*, N. Weeden* & W. Thompson*

TL;DR: The results, which indicate a high level of DNA restriction fragment length polymorphism in pea, suggest sufficient variation to permit the construction of a highly detailed linkage map. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1985 chapter

Photoregulation of nuclear-encoded transcripts: blue light regulation of specific transcript abundance

In K. E. Steinback, S. Bonitz, C. J. Arntzen, & L. Bogorad (Eds.), The Molecular Biology of the Photosynthetic Aparatus (pp. 367–376).

By: L. Kaufman, J. Watson, W. Briggs & W. Thompson

Ed(s): K. Steinback, S. Bonitz, C. Arntzen & L. Bogorad

TL;DR: Linkage analysis of familial data using RFLPs is making it possible to locate the chromosome segments carrying particular genes in cases where the molecular pathology of the defect is quite unknown and this is the first essential step in analysis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: June 8, 2019

1985 journal article

Phytochrome Control of Specific mRNA Levels in Developing Pea Buds : The Presence of Both Very Low Fluence and Low Fluence Responses

PLANT PHYSIOLOGY, 78(2), 388–393.

By: L. Kaufman*, W. Briggs* & W. Thompson*

TL;DR: Examination of phytochrome regulated changes in transcript abundance for 11 different light regulated mRNAs in developing pea buds found the very low fluence response is not far red reversible and in fact can be induced by far red light. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1985 chapter

Phytochrome Regulation of Plant Development at the Whole Plant, Physiological, and Molecular Levels

In Sensory Perception and Transduction in Aneural Organisms (pp. 265–280).

By: W. Briggs*, D. Mandoli*, J. Shinkle*, L. Kaufman*, J. Watson* & W. Thompson*

TL;DR: The effects of light, in which it provides an environmental cue to trigger a given response, rather than providing a direct energy source for the response itself, collectively define the field of photomorphogenesis. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1984 journal article

Different red light requirements for phytochrome-induced accumulation of Cab RNA and RbcS RNA

Science, 226(4681), 1447–1449.

By: L. Kaufman*, W. Thompson* & W. Briggs*

TL;DR: It is possible, therefore, that developing pea buds accumulate cab RNA before rbcS and that cab RNA is not subject to the normal end-of-day signals affecting many phytochrome responses. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1984 chapter

Evolutionary aspects of chloroplast genome expression and organization

In R. Hallick, L. A. Staehelin, J. P. Thornber, & A. R. Liss (Eds.), Biosynthesis of the Photosynthetic Apparatus: Molecular Biology, Development and Regulation (Vol. 14, pp. 273–283). New York: UCLA Symposia on Molecular and Cellular Biology, New Series.

By: J. Palmer, B. Osorio, J. Watson, H. Edwards, J. Dodd & W. Thompson

Ed(s): R. Hallick, L. Staehelin, J. Thornber & A. Liss

Source: NC State University Libraries
Added: April 11, 2019

1983 chapter

Chloroplast genes for components of the translational apparatus

In Carnegie Institution Washington Year Book (Vol. 82, pp. 24–26).

By: J. Watson, J. Palmer & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1983 conference paper

Cytosine methylation of ribosomal RNA genes and nucleolus organizer activity in wheat

Proceedings of the Kew Chromosome Conference II, 11–17.

By: R. Flavell, M. O'Dell & W. Thompson

Source: NC State University Libraries
Added: June 8, 2019

1983 journal article

DNase I sensitivity of transcriptionally active genes in intact nuclei and isolated chromatin of plants.

Proceedings of the National Academy of Sciences, 80(3), 815–819.

By: S. Spiker, M. Murray & W. Thompson*

MeSH headings : Cell Nucleus / ultrastructure; Chromosomal Proteins, Non-Histone / physiology; Deoxyribonucleases; Gene Expression Regulation; High Mobility Group Proteins; Nucleosomes / ultrastructure; Transcription, Genetic; Triticum
TL;DR: The chromatin structure that confers preferential DNase I sensitivity to transcriptionally active genes appears to be lost when the higher-ionic-strength method of preparation is used, and it is suggested that treatment of wheat nuclei with DN enzyme I causes the release of four prominent nonhistone chromosomal proteins that comigrate with wheat high mobility group proteins on NaDodSO4 gels. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1983 journal article

Mitochondrial DNA sequence evolution and homology to chloroplast DNA in angiosperms

UCLA Symposia on Molecular and Cellular Biology, 12, 467–477.

By: D. Stern, J. Palmer, W. Thompson & D. Lonsdale

Source: NC State University Libraries
Added: April 11, 2019

1983 journal article

Phytochrome control of RNA levels in developing pea and mung-bean leaves

Planta, 158(6), 487–500.

By: W. Thompson*, M. Everett*, N. Polans*, R. Jorgensen* & J. Palmer*

TL;DR: The results are consistent with the notion that chloroplast development is arrested at a later stage in dark-grown mung-bean leaves than in etiolated pea buds. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1982 journal article

Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost

Cell, 29(2), 537–550.

By: J. Palmer* & W. Thompson*

MeSH headings : Base Sequence; Biological Evolution; Chloroplasts; DNA / genetics; DNA Restriction Enzymes; Fabaceae / genetics; Nucleic Acid Hybridization; Plants / classification; Plants / genetics; Plants, Medicinal; Repetitive Sequences, Nucleic Acid
TL;DR: There is a relatively stable arrangement of sequences in those genomes with the inverted repeat and a much more dynamic arrangement in those that have lost it, including the possibility that the invertedrepeat may play a direct role in maintaining a conserved arrangement of chloroplast DNA sequences. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1982 chapter

Modes and tempos in the evolution of nuclear-encoded ribosomal RNA genes in legumes

In Carnegie Institution of Washington Year Book (Vol. 81, pp. 98–101).

By: R. Jorgensen, R. Cuellar & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1982 journal article

Novel evolutionary variation in transcription and location of two chloroplast genes

Nucleic Acids Research, 10(21), 6819–6832.

By: J. Palmer*, H. Edwards*, R. Jorgensen* & W. Thompson*

MeSH headings : Biological Evolution; Carboxy-Lyases / genetics; Chloroplasts / metabolism; Cloning, Molecular; Genes; Photosynthetic Reaction Center Complex Proteins; Photosystem II Protein Complex; Plant Proteins / genetics; Plants / genetics; Ribulose-Bisphosphate Carboxylase / genetics; Species Specificity; Transcription, Genetic
TL;DR: Major evolutionary changes in the types of transcripts produced by specific chloroplast genes, in particular those encoding the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase and a photosystem II polypeptide (PII). (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1982 journal article

Repeat sequence interspersion in coding DNA of peas does not reflect that in total pea DNA

Plant Molecular Biology, 1(2), 143–153.

By: M. Murray* & W. Thompson*

TL;DR: The data presented here demonstrate that sequences which code for mRNA are enriched in the small fraction of fragments which do not contain highly repetitive sequences, and indicate that those repeats which are closely associated with mRNA coding sequences belong to low copy number families characterized by an unusually low degree of sequence divergence. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: June 8, 2019

1981 journal article

Ancient repeated sequences in the pea and mung bean genomes and implications for genome evolution

Journal of Molecular Evolution, 17(1), 31–42.

By: M. Murray*, D. Peters* & W. Thompson*

TL;DR: An extension of a simple evolutionary model is proposed in which differences in genome organization are considered to reflect different rates of sequence amplification or genome turnover during evolution and accounts for some of the differences between typical plant and animal genomes. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1981 journal article

Clone banks of the mung bean, pea and spinach chloroplast genomes

Gene, 15(1), 21–26.

By: J. Palmer* & W. Thompson*

MeSH headings : Base Sequence; Chloroplasts / ultrastructure; Chromosome Mapping; DNA / genetics; DNA Restriction Enzymes / metabolism; DNA, Recombinant / analysis; Escherichia coli / genetics; Fabaceae; Plants, Medicinal; Plasmids; Vegetables
TL;DR: All but one of the PstI restriction fragments from mung bean, pea, and spinach chloroplast DNAs have been stably cloned into pBR322 and the identity of the cloned fragments to nativechloroplast DNA restriction fragments is demonstrated by restriction analysis and the ability to construct detailed restriction maps of the mungbean and pea chloropleft genomes. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1981 chapter

Complex organization of repetitive DNA families as analyzed with cloned DNA fragments

In Carnegie Institution of Washington Year Book (Vol. 80, pp. 81–82).

By: R. Cuellar & W. Thompson

Source: NC State University Libraries
Added: June 8, 2019

1981 chapter

DNA hybridization techniques for the study of plant evolution

In D. A. Young & D. S. Seigler (Eds.), Phytochemistry and Angiosperm Phylogeny (pp. 1–18). NY: Praeger Scientific.

By: H. Belford, W. Thompson & D. Stein

Ed(s): D. Young & D. Seigler

Source: NC State University Libraries
Added: April 11, 2019

1981 journal article

Evolutionary sequence divergence within repeated DNA families of higher plant genomes

Journal of Molecular Evolution, 17(2), 85–93.

By: R. Preisler* & W. Thompson*

MeSH headings : Biological Evolution; DNA / genetics; Escherichia coli / genetics; Fabaceae / genetics; Gene Amplification; Genes; Hot Temperature; Models, Genetic; Nucleic Acid Denaturation; Plants, Medicinal; Repetitive Sequences, Nucleic Acid
TL;DR: The melting data for DNA reassociated at two different temperatures provided strong evidence that Pisum sativum repeated families are mostly heterogeneous, while homogeneous families predominate in Vigna radiata, suggesting that the rate of sequence amplification has been higher in the evolutionary history of Pisum DNA. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1981 journal article

Evolutionary sequence divergence within repeated DNA families of higher plant genomes

Journal of Molecular Evolution, 17(2), 78–84.

By: R. Preisler* & W. Thompson*

MeSH headings : Biological Evolution; DNA / genetics; Fabaceae / genetics; Gene Amplification; Genes; Kinetics; Models, Genetic; Nucleic Acid Renaturation; Plants, Medicinal; Repetitive Sequences, Nucleic Acid
TL;DR: Evaluation of the kinetic data in light of the great diversity of repeated family copy numbers in both genomes enabled an interpretation of the results as consistent with hetereogenous families in Pisum and homogeneous families in Vigna, supported by the results of a thermal denaturation (melting) assay. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1981 journal article

Rearrangements in the chloroplast genomes of mung bean and pea

Proceedings of the National Academy of Sciences, 78(9), 5533–5537.

By: J. Palmer* & W. Thompson*

UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1981 journal article

Single copy DNA homologies in Atriplex. I. Cross reactivity estimates and the role of deletions in genome evolution

Heredity, 46(1), 91–108.

By: H. Belford* & W. Thompson*

TL;DR: The data show a large variation in cross reactivity for different species pairs which is best explained by postulating that these genomes were subject to extensive deletion during evolution of different lineages. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1981 journal article

Single copy DNA homologies in Atriplex. II. Hybrid thermal stabilities and molecular phylogeny

Heredity, 46(1), 109–122.

By: H. Belford* & W. Thompson*

TL;DR: The molecular data do not support the classical subgeneric distinction, and it is no longer necessary to postulate a polyphyletic origin for C4 photosynthesis within Atriplex. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1981 chapter

The nuclear genome: Structure and Function

In A. Marcus (Ed.), The Biochemistry of Plants (Vol. 6, pp. 1–81).

By: W. Thompson & M. Murray

Ed(s): A. Marcus

Source: NC State University Libraries
Added: April 11, 2019

1980 chapter

Contrasting Patterns of DNA Sequence Organization in Plants

In Genome Organization and Expression in Plants (pp. 1–15).

By: W. Thompson*, M. Murray* & R. Cuellar*

TL;DR: It seems logical to view plant genomes as particularly favorable material for studies on the origin and evolution of repeated sequences in eukaryotic genomes. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1980 chapter

Patterns of DNA sequence repetition and interspersion in higher plants

In I. Rubenstein, R. L. Phillips, C. E. Green, & B. G. Gegenbach (Eds.), Genetic Improvement of Crops (pp. 47–75). University of Minnesota Press.

By: W. Thompson, M. Murray, H. Belford & R. Cuellar

Ed(s): I. Rubenstein, R. Phillips, C. Green & B. Gegenbach

Source: NC State University Libraries
Added: April 11, 2019

1980 journal article

Rapid isolation of high molecular weight plant DNA

Nucleic Acids Research, 8(19), 4321–4326.

By: M. Murray* & W. Thompson*

MeSH headings : DNA / isolation & purification; Indicators and Reagents; Methods; Molecular Weight; Plants / analysis
TL;DR: A method is presented for the rapid isolation of high molecular weight plant DNA which is free of contaminants which interfere with complete digestion by restriction endonucleases, and which yields total cellular DNA. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1980 chapter

Sequence organization in pea and mung bean DNA and a model for genome evolution

In D. R. Davies & D. A. Hopwood (Eds.), The Plant Genome (pp. 31–45). Norwich, England: John Innes Charity.

By: W. Thompson & M. Murray

Ed(s): D. Davies & D. Hopwood

Source: NC State University Libraries
Added: April 11, 2019

1980 chapter

Studies on higher plant chloroplast and mitochondrial DNA

In Carnegie Institution of Washington Year Book (Vol. 79, pp. 120–123).

By: J. Palmer & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1979 chapter

Cloning of plant DNA. Comparison of five host strains of Escherichia coli

In Carnegie Institution of Washington Year Book (Vol. 78, pp. 215–217).

By: R. Cuellar, E. Selker & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1979 journal article

DNA sequence organization in the mung bean genome

Biochemistry, 18(23), 5259–5266.

By: M. Murray, J. Palmer, R. Cuellar & W. Thompson*

MeSH headings : Base Sequence; Chloroplasts / analysis; DNA; Genes; Kinetics; Nucleic Acid Renaturation; Plants / analysis
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1979 journal article

Studies on DNA sequence in the Osmundaceae

Journal of Molecular Evolution, 13(3), 215–232.

By: D. Stein*, W. Thompson* & H. Belford*

MeSH headings : Base Sequence; DNA / isolation & purification; Kinetics; Nucleic Acid Hybridization; Plants / analysis; Species Specificity
TL;DR: The data obtained suggest that the threeOsmunda species most likely arose more or less simultaneously from a common ancestor, and the rate of single copy sequence divergence in the ferns is comparable to that in the primates, although slower than that observed in other animal taxa. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1979 chapter

Studies on chloroplast DNA of mung bean and pea

In Carnegie Institution of Washington Year Book (Vol. 78, pp. 226–231).

By: J. Palmer, M. Murray & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1978 journal article

Application of higher derivative techniques to analysis of high-resolution thermal denaturation profiles of reassociated repetitive DNA.

Proceedings of the National Academy of Sciences, 75(12), 6026–6030.

By: R. Cuellar, G. Ford, W. Briggs & W. Thompson*

MeSH headings : Base Sequence; Computers; DNA / genetics; DNA, Bacterial / genetics; Escherichia coli; Hot Temperature; Hydrogen Bonding; Nucleic Acid Denaturation; Plants; Tetraethylammonium Compounds / pharmacology
TL;DR: High-resolution denaturation profiles of reassociated repetitive DNA sequences are analyzed by using a combination of higher derivative analysis and curve-fitting techniques to find at least five classes can be reproducibly distinguished in pea and mung bean DNAs. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1978 journal article

DNA sequence organization in the pea genome

Biochemistry, 17(26), 5781–5790.

By: M. Murray, R. Cuellar & W. Thompson*

MeSH headings : DNA / isolation & purification; Drug Stability; Genes; Hydroxyapatites; Kinetics; Molecular Weight; Nucleic Acid Renaturation; Plants / analysis
TL;DR: It is concluded that the major class of single-copy sequences in pea DNA has a modal length of about 300 nucleotides, which is in good agreement with previous chemical and cytophotometric measurements. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1978 chapter

Perspectives on the evolution of plant DNA

In Carnegie Institution of Washington Year Book (Vol. 77, pp. 310–316).

By: W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1977 chapter

Contaminants affecting plant DNA reassociation

In Carnegie Institution of Washington Year Book (Vol. 76, pp. 255–259).

By: M. Murray, H. Belford & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1977 chapter

In vitro labeling of single stranded DNA

In Carnegie Institution of Washington Year Book (Vol. 76, pp. 262–267).

By: M. Murray, H. Belford & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1977 chapter

Interspecific hybridization of fern DNA

In Carnegie Institution of Washington Year Book (Vol. 76, pp. 252–255).

By: D. Stein & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1977 journal article

Isolation of DNA from tannin-containing plants

Plant Science Letters, 11(3-4), 323–328.

By: D. Stein & W. Thompson*

TL;DR: A combination of techniques has been developed which permits purification of large amounts of DNA from plants containing high levels of tannins and brown pigments, permitting storage of field collections and eliminating the need for fresh material. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1977 chapter

Restriction endonuclease analysis of Agrobacterium plasmids

In Carnegie Institution of Washington Year Book (Vol. 76, pp. 267–273).

By: C. Rogler, S. Cohen & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1977 chapter

Single-strand DNA fragment length determination by alkaline agarose electrophoresis: Applications for DNA renaturation studies

In Carnegie Institution of Washington Year Book (Vol. 76, pp. 259–262).

By: M. Murray & W. Thompson

Source: NC State University Libraries
Added: April 21, 2019

1976 journal article

Aggregate Formation from Short Fragments of Plant DNA

PLANT PHYSIOLOGY, 57(4), 617–622.

By: W. Thompson*

TL;DR: It is suggested, as a working hypothesis, that much of the repetitive sequence DNA in peas is contained in regions considerably longer than 300 base pairs, analogous to the large hyperpolymers which have been observed in extensively reassociated prokaryotic DNA. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1976 chapter

Plasmid modification associated with loss and acquisition of virulence in Agrobacterium tumefaciens

In Carnegie Institution of Washington Year Book (Vol. 75, pp. 367–373).

By: C. Rogler & W. Thompson

Source: NC State University Libraries
Added: June 8, 2019

1975 journal article

DNA Hybridization and Evolutionary Relationships in Three Osmunda Species

Science, 189(4206), 888–890.

By: D. Stein* & W. Thompson*

TL;DR: Findings under conditions permitting extensive reassociation, measurements of the extent of inter-specific reaction and the thermal stability of the hybrid molecules indicated that O. claytoniana L.interrupted fern shares more DNA homology with O. cinnamomea L. (cinnamon fern) than it does with O., regalis L. regalis (royal fern). (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1974 journal article

Differential reactivity of the red-and far-red-absorbing forms of phytochrome to [14C] N-ethyl maleimide

Planta, 117(4), 367–372.

By: G. Gardner*, W. Thompson* & W. Briggs*

TL;DR: The red-absorbing form (Pr) and the far-red absorbing form (Pfr) of undergraded, high-molecular-weight phytochrome from rye seedlings were examined for their reactivity toward N-ethyl-[14C]maleimide ([14C]-NEM). (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1972 journal article

Effect of light and gibberellin on RNA synthesis in pea stem tissue as studied by DNA/RNA hybridization

Plant Physiology, 50, 289–292.

By: W. Thompson* & R. Cleland*

TL;DR: Gibberellin is similar to auxin in its ability to induce stem growth without causing detectable changes in hybridizable RNA. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2019

1971 journal article

Auxin and Ribonucleic Acid Synthesis in Pea Stem Tissue as Studied by Deoxyribonucleic Acid-Ribonucleic Acid Hybridization

PLANT PHYSIOLOGY, 48(6), 663–670.

By: W. Thompson* & R. Cleland*

TL;DR: No change in hybridizable RNA of stem sections in response to auxin could be detected over a 2- to 24-hour period, regardless of the auxin level employed, and when large doses of auxin were applied to intact pea seedlings, definite changes in the hybridizableRNA of stem tissue were detected both 8 and 24 hours after treatment. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: March 2, 2019

1971 chapter

Macromolecule synthesis and wall extensibility in relation to the mechanism of auxin-induced cell elongation

In Plant Growth Substances 1970, VII International Congress on Plant Growth Substances.

By: R. Cleland, W. Thompson, P. Haughton & D. Rayle

Event: at Canberra, Australia

Source: NC State University Libraries
Added: April 11, 2019

Employment

Updated: June 4th, 2018 11:10

1986 - present

North Carolina State University Raleigh, NC, US
Professor Plant and Microbial Biology

1974 - 1986

Carnegie Institution of Washington Stanford, CA, US
Faculty Member Plant Biology

1972 - 1974

University of Massachusetts Amherst Amherst, MA, US
Assistant Professor Botany

Education

Updated: June 4th, 2018 11:04

1970 - 1972

Harvard University Cambridge, MA, US
(postdoc) Biology

1966 - 1970

University of Washington Seattle, WA, US
PhD Botany

1963 - 1966

Princeton University Princeton, NJ, US
A. B. Biology

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