Wesley Legant

Works (10)

Updated: April 5th, 2024 11:23

2023 journal article

Patient-derived extracellular matrix demonstrates role of COL3A1 in blood vessel mechanics


By: E. Doherty n, W. Aw n, E. Warren n, M. Hockenberry*, C. Whitworth*, G. Krohn n, S. Howell*, B. Diekman n ...

author keywords: Vascular Ehlers-Danlos syndrome; Cell derived matrix; Collagen III; Mechanotransduction; ECM mechanics; Viscoelasticity
TL;DR: This work generates fibrous, collagen-rich ECM from primary donor cells from patients with vascular Ehlers-Danlos syndrome, a disease caused by mutations in the gene that encodes collagen III, and observes that ECM grown from vEDS patients is characterized by unique mechanical signatures, including altered viscoelastic properties. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: August 7, 2023

2023 journal article

u-track3D: Measuring, navigating, and validating dense particle trajectories in three dimensions


TL;DR: U-track3D is described, a software package that extends the versatile u-track framework established in 2D to address the specific challenges of 3D particle tracking and presents the concept of dynamic region of interest (dynROI), which allows an experimenter to interact with dynamic 3D processes in 2D views amenable to visual inspection. (via Semantic Scholar)
Source: Web Of Science
Added: February 12, 2024

2022 journal article

A quantitative analysis of various patterns applied in lattice light sheet microscopy


By: Y. Shi n, T. Daugird* & W. Legant*

MeSH headings : Microscopy; Normal Distribution; Photobleaching
Source: Web Of Science
Added: August 29, 2022

2022 article

Cells in the mechanical spotlight

Hockenberry, M. A., & Legant, W. R. (2022, October 4). BIOPHYSICAL JOURNAL, Vol. 121, pp. 3571–3572.

By: M. Hockenberry* & W. Legant n

UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Web Of Science
Added: November 28, 2022

2022 journal article

Superresolution microscopy reveals actomyosin dynamics in medioapical arrays


By: R. Moore n, S. Fogerson*, U. Tulu*, J. Yu*, A. Cox*, M. Sican*, D. Li*, W. Legant n ...

MeSH headings : Actin Cytoskeleton / metabolism; Actins / metabolism; Actomyosin / metabolism; Animals; Drosophila / metabolism; Microscopy; Myosins / metabolism
TL;DR: These images offer unparalleled resolution of F-actin in embryonic tissue, show that medioapical arrays are tightly apposed to the plasma membrane and are continuous with meshworks of lamellar F-actsin, and constitute modified cell cortex. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: October 24, 2022

2021 article

A multi-functional microfluidic device compatible with widefield and light sheet microscopy

Moore, R. P., Ellen C. O'Shaughnessy, Shi, Y., Nogueira, A. T., Heath, K. M., Hahn, K. M., & Legant, W. R. (2021, November 22). LAB ON A CHIP.

By: R. Moore n, . Ellen C. O'Shaughnessy*, Y. Shi n, A. Nogueira*, K. Heath n, K. Hahn*, W. Legant n

MeSH headings : Lab-On-A-Chip Devices; Lenses; Microfluidics; Microscopy; Refractometry
Source: Web Of Science
Added: December 13, 2021

2021 article

Deep learning enables fast and dense single-molecule localization with high accuracy

Speiser, A., Mueller, L.-R., Hoess, P., Matti, U., Obara, C. J., Legant, W. R., … Turaga, S. C. (2021, September 3). NATURE METHODS.

MeSH headings : Animals; COS Cells; Chlorocebus aethiops; Databases, Factual; Deep Learning; Image Processing, Computer-Assisted / methods; Single Molecule Imaging / methods; Software
TL;DR: DECODE (deep context dependent), a computational tool that can localize single emitters at high density in three dimensions with highest accuracy for a large range of imaging modalities and conditions, which outperforms available methods and enables fast live-cell SMLM of dynamic processes. (via Semantic Scholar)
Source: Web Of Science
Added: September 13, 2021

2021 article

Phase separation drives aberrant chromatin looping and cancer development

Ahn, J. H., Davis, E. S., Daugird, T. A., Zhao, S., Quiroga, I. Y., Uryu, H., … Wang, G. G. (2021, June 23). NATURE.

By: J. Ahn*, E. Davis*, T. Daugird*, S. Zhao*, I. Quiroga*, H. Uryu*, J. Li*, A. Storey* ...

MeSH headings : Animals; Carcinogenesis; Chromatin / genetics; Female; HEK293 Cells; HeLa Cells; Homeodomain Proteins / genetics; Humans; Intrinsically Disordered Proteins / genetics; Mice; Mice, Inbred BALB C; Neoplasms / genetics; Neoplasms / pathology; Nuclear Pore Complex Proteins / genetics; Oncogene Proteins, Fusion / genetics; Transcription Factors / genetics; Transcriptional Activation; Translocation, Genetic
TL;DR: A proof-of-principle example in which cancer acquires mutation to establish oncogenic transcription factor condensates via phase separation, which simultaneously enhances their genomic targeting and induces organization of aberrant three-dimensional chromatin structure during tumourous transformation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: July 6, 2021

2019 journal article

High-resolution imaging reveals how the spindle midzone impacts chromosome movement

JOURNAL OF CELL BIOLOGY, 218(8), 2529–2544.

By: M. Pamula*, L. Carlini*, S. Forth*, P. Verma*, S. Suresh*, W. Legant n, A. Khodjakoy, E. Betzig*, T. Kapoor*

MeSH headings : Anaphase; Cell Cycle Proteins / metabolism; Chromosome Segregation; Chromosomes, Human / metabolism; Fluorescence Recovery After Photobleaching; HeLa Cells; Humans; Imaging, Three-Dimensional; Microtubule-Associated Proteins / metabolism; Microtubules / metabolism; Movement; Mutation / genetics; Spindle Apparatus / metabolism
TL;DR: The assembly dynamics of midzone microtubule bundles during anaphase are examined and how chromosome segregation is impacted by aberrant bundle assembly is examined. (via Semantic Scholar)
Source: Web Of Science
Added: August 19, 2019

2018 article

Improving probes for super-resolution

Moore, R. P., & Legant, W. R. (2018, September). NATURE METHODS, Vol. 15, pp. 659–660.

By: R. Moore n & W. Legant n

MeSH headings : DNA; Microscopy, Fluorescence; Oligonucleotides
TL;DR: Chemically modified DNA aptamers enable quantitative super-resolution imaging and improve the quality of high-resolution X-ray diffraction analysis in the nucleus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: October 19, 2018

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