Works (9)

Updated: July 5th, 2023 15:48

2018 article

Sequential Phosphopeptide Enrichment for Phosphoproteome Analysis of Filamentous Fungi: A Test Case Using Magnaporthe oryzae

PLANT PATHOGENIC FUNGI AND OOMYCETES: METHODS AND PROTOCOLS, Vol. 1848, pp. 81–91.

By: Y. Oh n, W. Franck n & R. Dean n

author keywords: Mass spectrometry; Phosphopeptide enrichment; Magnaporthe oryzae; Iron-IMAC; TiO2; Fungus
MeSH headings : Chromatography, Affinity; Chromatography, Liquid; Computational Biology / methods; Data Analysis; Fungal Proteins; Humans; Magnaporthe / metabolism; Phosphopeptides / chemistry; Phosphopeptides / isolation & purification; Phosphopeptides / metabolism; Phosphoproteins / chemistry; Phosphoproteins / isolation & purification; Phosphoproteins / metabolism; Proteome; Proteomics / methods; Tandem Mass Spectrometry; Titanium / chemistry
TL;DR: An effective protocol for phosphopeptide enrichment using an Iron-IMAC resin in combination with titanium dioxide (TiO2) beads from trypsin digested protein samples of the filamentous fungus Magnaporthe oryzae is described. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: May 13, 2019

2017 journal article

Identification and characterization of suppressors of plant cell death (SPD) effectors from Magnaporthe oryzae

MOLECULAR PLANT PATHOLOGY, 18(6), 850–863.

By: W. Sharpee n, Y. Oh n, M. Yi*, W. Franck n, A. Eyre n, L. Okagaki n, B. Valent*, R. Dean n

author keywords: effector; rice blast; suppressor
MeSH headings : Fungal Proteins / genetics; Fungal Proteins / metabolism; Host-Pathogen Interactions; Magnaporthe / metabolism; Magnaporthe / pathogenicity; Plant Diseases / genetics; Plant Diseases / microbiology; Plant Leaves / metabolism; Plant Leaves / microbiology; Tobacco / metabolism; Tobacco / microbiology
TL;DR: A series of effectors that probably act at different stages of the infection process and contribute to the virulence of M. oryzae are identified, some of which were previously unknown or whose function was unknown. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Comparative transcriptomic analysis of symbiotic Bradyrhizobium japonicum

Symbiosis, 63(3), 123–135.

By: S. Franck, W. Franck, S. Birke, W. Chang, D. Sangurdekar, E. Cytryn, T. Joshi, M. Sadowsky ...

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Soybean ureases, but not that of Bradyrhizobium japonicum, are involved in the process of soybean root nodulation

Journal of Agricultural and Food Chemistry, 62(16), 3517–3524.

By: M. Medeiros-Silva, W. Franck, M. Borba, S. Pizzato, K. Strodtman, D. Emerich, G. Stacey, J. Poacco, C. Carlini

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

Temporal Analysis of theMagnaporthe OryzaeProteome During Conidial Germination and Cyclic AMP (cAMP)-mediated Appressorium Formation

Molecular & Cellular Proteomics, 12(8), 2249–2265.

By: W. Franck n, E. Gokce n, Y. Oh n, D. Muddiman n & R. Dean n

MeSH headings : Cyclic AMP / metabolism; Fungal Proteins / metabolism; Magnaporthe / growth & development; Magnaporthe / metabolism; Mitochondrial Proteins / metabolism; Peptide Hydrolases / metabolism; Proteome; Spores, Fungal / growth & development; Spores, Fungal / metabolism
TL;DR: The data presented here provide the first comprehensive view of the M. oryzae proteome during early infection-related development and highlight biological processes important for pathogenicity. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2012 journal article

In-Depth Analysis of the Magnaporthe oryzae Conidial Proteome

JOURNAL OF PROTEOME RESEARCH, 11(12), 5827–5835.

By: E. Gokce n, W. Franck n, Y. Oh n, R. Dean n & D. Muddiman n

author keywords: proteomics; mass spectrometry; Magnaporthe oryzae; FASP; statistical tests; differential protein expression
MeSH headings : Cell Nucleus / chemistry; Chemical Fractionation / methods; Chromatography, Liquid / methods; Cytoplasm / chemistry; Fungal Proteins / analysis; Fungal Proteins / chemistry; Magnaporthe / chemistry; Mass Spectrometry / methods; Nanotechnology; Nuclear Proteins / analysis; Nuclear Proteins / chemistry; Peptides / analysis; Peptides / chemistry; Proteome / analysis; Proteome / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spores, Fungal / chemistry; Time Factors
TL;DR: The identification of 2912 conidial proteins at a 1% protein false discovery rate (FDR) is reported and the most extensive study performed on M. oryzae conidia to date is presented, suggesting the detected proteins build a representative portion of the predicted proteome. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Polyubiquitin Is Required for Growth, Development and Pathogenicity in the Rice Blast Fungus Magnaporthe oryzae

PLoS ONE, 7(8), e42868.

By: Y. Oh n, W. Franck n, S. Han*, A. Shows n, E. Gokce n, D. Muddiman n, R. Dean n

Ed(s): K. Nielsen

MeSH headings : Boronic Acids / pharmacology; Bortezomib; Cluster Analysis; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Germination / genetics; Magnaporthe / genetics; Magnaporthe / growth & development; Magnaporthe / pathogenicity; Mutation; Nitrogen / metabolism; Oryza / microbiology; Plant Diseases / microbiology; Polyubiquitin / genetics; Polyubiquitin / metabolism; Proteasome Inhibitors / pharmacology; Proteolysis / drug effects; Proteomics; Pyrazines / pharmacology; Ubiquitination
TL;DR: This study suggests that ubiquitination of target proteins plays an important role in nutrient assimilation, development and pathogenicity of M. oryzae. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2011 journal article

Evaluation of Normalization Methods on GeLC-MS/MS Label-Free Spectral Counting Data to Correct for Variation during Proteomic Workflows

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(12), 2199–2208.

By: E. Gokce n, C. Shuford n, W. Franck n, R. Dean n & D. Muddiman n

author keywords: Label-free; Proteomics; Mass spectrometry; Total spectral count normalization; Normalized spectral abundance factor; Quantification; Spectral counting; Significance test; Fold-change; GeLC-MS/MS; nanoLC-MS; Orbitrap XL
TL;DR: The correlation between SpCs of the same proteins across the different data sets was investigated and it was reported that TSpC normalization and NSAF normalization yielded almost ideal slopes of unity for normalized SpC versus average normalized SpCs, while NSP did not afford effective corrections of the unnormalized data. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics

ANALYTICAL CHEMISTRY, 82(20), 8696–8702.

By: T. Collier n, P. Sarkar n, W. Franck n, B. Rao n, R. Dean n & D. Muddiman n

MeSH headings : Amino Acids / analysis; Animals; Cell Line; Humans; Isotope Labeling / methods; Mice; Proteomics / methods
TL;DR: A direct comparison of SILAC to label-free quantification by spectral counting of an identical set of data from the bottom-up proteomic analysis of human embryonic stem cells, finding that both strategies result in a similar number of protein identifications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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