William L. Franck
Works (9)
Updated: July 5th, 2023 15:48
2018 article
Sequential Phosphopeptide Enrichment for Phosphoproteome Analysis of Filamentous Fungi: A Test Case Using Magnaporthe oryzae
PLANT PATHOGENIC FUNGI AND OOMYCETES: METHODS AND PROTOCOLS, Vol. 1848, pp. 81–91.
author keywords: Mass spectrometry; Phosphopeptide enrichment; Magnaporthe oryzae; Iron-IMAC; TiO2; Fungus
MeSH headings : Chromatography, Affinity; Chromatography, Liquid; Computational Biology / methods; Data Analysis; Fungal Proteins; Humans; Magnaporthe / metabolism; Phosphopeptides / chemistry; Phosphopeptides / isolation & purification; Phosphopeptides / metabolism; Phosphoproteins / chemistry; Phosphoproteins / isolation & purification; Phosphoproteins / metabolism; Proteome; Proteomics / methods; Tandem Mass Spectrometry; Titanium / chemistry
TL;DR:
An effective protocol for phosphopeptide enrichment using an Iron-IMAC resin in combination with titanium dioxide (TiO2) beads from trypsin digested protein samples of the filamentous fungus Magnaporthe oryzae is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 13, 2019
2016 journal article
Identification and characterization of suppressors of plant cell death (SPD) effectors from Magnaporthe oryzae
MOLECULAR PLANT PATHOLOGY, 18(6), 850–863.
author keywords: effector; rice blast; suppressor
MeSH headings : Fungal Proteins / genetics; Fungal Proteins / metabolism; Host-Pathogen Interactions; Magnaporthe / metabolism; Magnaporthe / pathogenicity; Plant Diseases / genetics; Plant Diseases / microbiology; Plant Leaves / metabolism; Plant Leaves / microbiology; Tobacco / metabolism; Tobacco / microbiology
TL;DR:
A series of effectors that probably act at different stages of the infection process and contribute to the virulence of M. oryzae are identified, some of which were previously unknown or whose function was unknown.
(via Semantic Scholar)
open access via onlinelibrary.wiley.com (publisher PDF)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
Comparative transcriptomic analysis of symbiotic Bradyrhizobium japonicum
Symbiosis, 63(3), 123–135.
Source: NC State University Libraries
Added: August 6, 2018
2014 journal article
Soybean ureases, but not that of Bradyrhizobium japonicum, are involved in the process of soybean root nodulation
Journal of Agricultural and Food Chemistry, 62(16), 3517–3524.
Source: NC State University Libraries
Added: August 6, 2018
2013 journal article
Temporal Analysis of theMagnaporthe OryzaeProteome During Conidial Germination and Cyclic AMP (cAMP)-mediated Appressorium Formation
Molecular & Cellular Proteomics, 12(8), 2249–2265.
MeSH headings : Cyclic AMP / metabolism; Fungal Proteins / metabolism; Magnaporthe / growth & development; Magnaporthe / metabolism; Mitochondrial Proteins / metabolism; Peptide Hydrolases / metabolism; Proteome; Spores, Fungal / growth & development; Spores, Fungal / metabolism
TL;DR:
The data presented here provide the first comprehensive view of the M. oryzae proteome during early infection-related development and highlight biological processes important for pathogenicity.
(via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
2012 journal article
In-Depth Analysis of the Magnaporthe oryzae Conidial Proteome
JOURNAL OF PROTEOME RESEARCH, 11(12), 5827–5835.
author keywords: proteomics; mass spectrometry; Magnaporthe oryzae; FASP; statistical tests; differential protein expression
MeSH headings : Cell Nucleus / chemistry; Chemical Fractionation / methods; Chromatography, Liquid / methods; Cytoplasm / chemistry; Fungal Proteins / analysis; Fungal Proteins / chemistry; Magnaporthe / chemistry; Mass Spectrometry / methods; Nanotechnology; Nuclear Proteins / analysis; Nuclear Proteins / chemistry; Peptides / analysis; Peptides / chemistry; Proteome / analysis; Proteome / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spores, Fungal / chemistry; Time Factors
TL;DR:
The identification of 2912 conidial proteins at a 1% protein false discovery rate (FDR) is reported and the most extensive study performed on M. oryzae conidia to date is presented, suggesting the detected proteins build a representative portion of the predicted proteome.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Polyubiquitin Is Required for Growth, Development and Pathogenicity in the Rice Blast Fungus Magnaporthe oryzae
PLoS ONE, 7(8), e42868.
Ed(s): K. Nielsen
MeSH headings : Boronic Acids / pharmacology; Bortezomib; Cluster Analysis; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Germination / genetics; Magnaporthe / genetics; Magnaporthe / growth & development; Magnaporthe / pathogenicity; Mutation; Nitrogen / metabolism; Oryza / microbiology; Plant Diseases / microbiology; Polyubiquitin / genetics; Polyubiquitin / metabolism; Proteasome Inhibitors / pharmacology; Proteolysis / drug effects; Proteomics; Pyrazines / pharmacology; Ubiquitination
TL;DR:
This study suggests that ubiquitination of target proteins plays an important role in nutrient assimilation, development and pathogenicity of M. oryzae.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
2011 journal article
Evaluation of Normalization Methods on GeLC-MS/MS Label-Free Spectral Counting Data to Correct for Variation during Proteomic Workflows
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(12), 2199–2208.
author keywords: Label-free; Proteomics; Mass spectrometry; Total spectral count normalization; Normalized spectral abundance factor; Quantification; Spectral counting; Significance test; Fold-change; GeLC-MS/MS; nanoLC-MS; Orbitrap XL
TL;DR:
The correlation between SpCs of the same proteins across the different data sets was investigated and it was reported that TSpC normalization and NSAF normalization yielded almost ideal slopes of unity for normalized SpC versus average normalized SpCs, while NSP did not afford effective corrections of the unnormalized data.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics
ANALYTICAL CHEMISTRY, 82(20), 8696–8702.
MeSH headings : Amino Acids / analysis; Animals; Cell Line; Humans; Isotope Labeling / methods; Mice; Proteomics / methods
TL;DR:
A direct comparison of SILAC to label-free quantification by spectral counting of an identical set of data from the bottom-up proteomic analysis of human embryonic stem cells, finding that both strategies result in a similar number of protein identifications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
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