Works (46)

Updated: April 3rd, 2024 20:01

2023 article

Differential SW16.1 allelic effects and genetic backgrounds contributed to increased seed weight after soybean domestication

Chen, X., Liu, C., Guo, P., Hao, X., Pan, Y., Zhang, K., … Gai, J. (2023, May 12). JOURNAL OF INTEGRATIVE PLANT BIOLOGY, Vol. 5.

By: X. Chen*, C. Liu*, P. Guo*, X. Hao*, Y. Pan*, K. Zhang*, W. Liu n, L. Zhao* ...

author keywords: domestication; natural variation; positional cloning; QTL; seed weight; soybean; SW16; 1
TL;DR: The results suggest that eliminating negative alleles from G. soja led to a higher frequency of positive alleles and changed genetic backgrounds in G. max, which contributed to larger seeds in cultivated soybean after domestication from wild soybean. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, ORCID
Added: May 22, 2023

2023 journal article

Engineered Cleistogamy in Camelina sativa for bioconfinement

HORTICULTURE RESEARCH, 10(2).

By: D. Huang n, L. Gao n, J. McAdams n, F. Zhao n, H. Lu n, Y. Wu n, J. Martin n, S. Sherif* ...

TL;DR: The engineered cleistogamy using the overexpressed PpJAZ1 is a highly effective bioconfinement strategy to limit PMGF from transgenic camelina, and could be used for bioconFinement in other dicot species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: March 13, 2023

2023 journal article

Plant Promoters and Terminators for High-Precision Bioengineering

BioDesign Research.

TL;DR: The function and features of plant core promoters, proximal and distal promoters, and terminators are reviewed, and their effects on and benchmarking strategies for regulating gene expression are reviewed. (via Semantic Scholar)
Source: ORCID
Added: July 8, 2023

2022 article

BrABF3 promotes flowering through the direct activation of CONSTANS transcription in pak choi

Zhang, C., Zhou, Q., Liu, W., Wu, X., Li, Z., Xu, Y., … Liu, T. (2022, May 6). PLANT JOURNAL, Vol. 5.

author keywords: abscisic acid; BrABF3; CO; flowering time; pak choi
MeSH headings : Abscisic Acid / metabolism; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Brassica rapa / genetics; Brassica rapa / metabolism; DNA-Binding Proteins / metabolism; Gene Expression Regulation, Plant / genetics; Transcription Factors / genetics; Transcription Factors / metabolism
TL;DR: Yeast one-hybrid assay suggests that ABA could accelerate the floral transition by directly activating BrCO transcription through BrABF3 in Pak choi. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: ORCID, Web Of Science
Added: April 21, 2022

2022 journal article

Coordinated transcriptional regulation of the carotenoid biosynthesis contributes to fruit lycopene content in high-lycopene tomato genotypes

Horticulture Research, 9.

By: J. Duduit n, P. Kosentka n, M. Miller n, B. Blanco-Ulate*, M. Lenucci*, D. Panthee n, P. Perkins-Veazie n, W. Liu n

TL;DR: This is the first systematic investigation of the role of the complete pathway genes in regulating fruit lycopene biosynthesis across many HLYs, and enables tomato breeding and gene editing for increased fruit Lycopene content. (via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref
Added: August 1, 2022

2022 journal article

Functional analysis of soybean cyst nematode-inducible synthetic promoters and their regulation by biotic and abiotic stimuli in transgenic soybean (Glycine max)

FRONTIERS IN PLANT SCIENCE, 13.

By: M. Sultana*, M. Mazarei*, R. Millwood*, W. Liu n, T. Hewezi* & C. Stewart Jr

author keywords: transgenic soybean; synthetic promoters; plant pathogenic nematodes; biotic and abiotic stresses; GUS
TL;DR: Histochemical GUS analyses of transgenic soybean plants containing the individual synthetic promoter::GUS construct revealed that under unstressed condition, no GUS activity is present in leaves and roots, and insights are provided into the potential applications of synthetic promoter engineering for conditional expression of transgenes leading to transgenic crop development for resistance improvement in plant. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: October 31, 2022

2022 review

Genotype-independent plant transformation

[Review of ]. HORTICULTURE RESEARCH, 9.

By: N. Maren n, H. Duan*, K. Da n, G. Yencho n, T. Ranney n & W. Liu n

TL;DR: This review will discuss the recent advances in the use of regulatory genes in plant transformation and regeneration, and their potential to facilitate genotype-independent plant regeneration and regeneration. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 1, 2022

2022 journal article

Identification of Novel Genomic Regions for Bacterial Leaf Pustule (BLP) Resistance in Soybean (Glycine max L.) via Integrating Linkage Mapping and Association Analysis

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(4).

By: F. Zhao*, W. Cheng*, Y. Wang*, X. Gao*, D. Huang n, J. Kong*, A. Antwi-Boasiako*, L. Zheng* ...

author keywords: linkage mapping; GWAS; qPCR; Xanthornonas axonopodis pv; glycines; bacterial leaf pustule; soybean
MeSH headings : Chromosome Mapping / methods; Chromosomes, Plant / genetics; Disease Resistance / genetics; Genes, Plant / genetics; Genome-Wide Association Study / methods; Genomics / methods; Genotype; Phenotype; Plant Breeding / methods; Plant Diseases / genetics; Polymorphism, Single Nucleotide / genetics; Quantitative Trait Loci / genetics; Soybeans / genetics
TL;DR: Results indicate that Glyma.17G086300 is a potential candidate gene for rxp and the QTLs and QTNs identified in this study will be useful for marker development for the breeding of Xag-resistant soybean cultivars. (via Semantic Scholar)
Sources: ORCID, Web Of Science
Added: February 19, 2022

2022 journal article

The genomic and bulked segregant analysis of Curcuma alismatifolia revealed its diverse bract pigmentation

ABIOTECH, 3(3), 178–196.

By: X. Liao*, Y. Ye*, X. Zhang*, D. Peng*, M. Hou*, G. Fu*, J. Tan*, J. Zhao* ...

author keywords: Anthocyanin synthesis; Siam tulip; Floriculture; Zingiberaceae; Genome evolution
TL;DR: This work provides data for understanding the mechanism of bract pigmentation and will accelerate breeding in developing novel cultivars with richly colored bracts in C. alismatifolia and related species and understand the variation in the evolution of the Zingiberaceae family. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: January 17, 2023

2022 journal article

Tissue Culture and Regeneration of Three Rose Cultivars

HORTSCIENCE, 57(11), 1430–1435.

By: D. Harmon n, D. Touchell n, T. Ranney n, K. Da n & W. Liu n

author keywords: auxin; carbohydrate; cytokinin; plant regeneration; micropropagation; somatic embryogenesis; Rosa ?hybrida
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, ORCID
Added: November 7, 2022

2021 journal article

An optimized protocol for stepwise optimization of real-time RT-PCR analysis

HORTICULTURE RESEARCH, 8(1).

By: F. Zhao*, N. Maren n, P. Kosentka n, Y. Liao n, H. Lu n, J. Duduit n, D. Huang n, H. Ashrafi n ...

TL;DR: This work proposed an optimized approach to sequentially optimizing primer sequences, annealing temperatures, primer concentrations, and cDNA concentration range for each reference (and target) gene and validated their utility under varying abiotic stress conditions. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 1, 2021

2021 article

FaMYB9 is involved in the regulation of C6 volatile biosynthesis in strawberry (vol 293, 110422, 2020)

Lu, H., Luo, Z., Wang, L., Liu, W., Li, D., Belwal, T., … Li, L. (2021, December). PLANT SCIENCE, Vol. 313.

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID
Added: November 29, 2021

2021 journal article

Rational design and testing of abiotic stress-inducible synthetic promoters from poplar cis-regulatory elements

PLANT BIOTECHNOLOGY JOURNAL, 19(7), 1354–1369.

author keywords: synthetic biology; synthetic promoters; de novo motif analysis; computational design; abiotic stress; cis‐ regulatory elements; poplar; bioenergy
MeSH headings : Gene Expression Regulation, Plant / genetics; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Promoter Regions, Genetic / genetics; Stress, Physiological / genetics
TL;DR: The synthetic biology design pipeline resulted in five synthetic promoters that outperformed endogenous promoters in transgenic plants, and these five promoters were stably transformed into Arabidopsis thaliana to study inducibility in whole plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: ORCID, Web Of Science
Added: January 21, 2021

2021 journal article

Reproductive developmental transcriptome analysis of Tripidium ravennae (Poaceae)

BMC GENOMICS, 22(1).

By: N. Maren n, F. Zhao n, R. Aryal n, D. Touchell n, W. Liu n, T. Ranney n, H. Ashrafi n

author keywords: Tripidium ravennae; Transcriptome sequencing; Differential gene expression; Next-generation sequencing; Floral transition; Flowering; Inflorescence development; Reproduction; RNA sequencing; Seed development
MeSH headings : Gene Expression Profiling; Gene Expression Regulation, Plant; Inflorescence; Plant Breeding; Poaceae
TL;DR: This research identified differentially expressed transcripts associated with floral induction, inflorescence development, and seed development in T. ravennae, providing insights into the molecular regulation of reproductive development and providing a foundation for future investigations and analyses. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: July 19, 2021

2021 review

Synthetic biology approaches in regulation of targeted gene expression

[Review of ]. CURRENT OPINION IN PLANT BIOLOGY, 63.

By: D. Huang n, P. Kosentka n & W. Liu n

author keywords: Synthetic biology; Targeted gene expression; Transcriptional regulation; Post-transcriptional regulation; Translational regulation
MeSH headings : Gene Expression Regulation; Plants / genetics; Plants / metabolism; Promoter Regions, Genetic; Synthetic Biology; Transcription Factors / metabolism; Transcription, Genetic
TL;DR: Recent advances in plant synthetic biology for the regulation of gene expression at multiple levels are discussed, and their future directions are highlighted. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science
Added: May 24, 2021

2021 journal article

Synthetic biology approaches in regulation of targeted gene expression

Current Opinion in Plant Biology.

Wusheng Liu

Source: ORCID
Added: May 9, 2021

2020 journal article

FaMYB9 is involved in the regulation of C6 volatile biosynthesis in strawberry

PLANT SCIENCE, 293.

author keywords: C6 volatiles; FaMYB9; LOX pathway; Metabolomics; Proteomics; Ripening
MeSH headings : Biosynthetic Pathways / genetics; Fragaria / genetics; Fragaria / metabolism; Fruit / chemistry; Fruit / genetics; Fruit / growth & development; Fruit / metabolism; Gene Expression Regulation, Plant; Genes, Plant / genetics; Metabolomics; Plant Proteins / genetics; Plant Proteins / metabolism; Proteomics; RNA, Messenger / metabolism; Transcriptome; Volatile Organic Compounds / chemistry; Volatile Organic Compounds / metabolism
TL;DR: The large-scale untargeted proteomic and metabolomic studies were conducted in strawberry (Fragaria × ananassa) cv. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: April 6, 2020

2020 journal article

Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells

Plant Cell Reports, 39(2), 245–257.

By: W. Liu n, M. Rudis*, M. Cheplick*, R. Millwood*, J. Yang*, C. Ondzighi-Assoume*, G. Montgomery*, K. Burris n ...

author keywords: Protein delivery; Cas9; gRNA ribonucleoprotein; Lipofection; Biolistics; Genome editing; Protoplast; Tobacco; BY2
MeSH headings : Agrobacterium; Biolistics / methods; CRISPR-Cas Systems; Cell Line; DNA; Gene Editing / methods; Gene Expression Regulation, Plant; Genome, Plant; Mutagenesis; Plant Cells / metabolism; Plants, Genetically Modified; Protoplasts; RNA, Guide, Kinetoplastida / metabolism; Ribonucleoproteins / genetics; Ribonucleoproteins / metabolism; Tobacco / genetics
TL;DR: The results showed that the newly developed lipofection-mediated transfection approach is robust for the use of the DNA-free Cas9/gRNA technology for genome editing in plant cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: ORCID
Added: January 5, 2021

2020 journal article

Transcription factor LkWOX4 is involved in adventitious root development in Larix kaempferi

GENE, 758.

By: H. Wang*, Y. Xie*, W. Liu n, G. Tao*, C. Sun*, X. Sun*, S. Zhang*

author keywords: Larch; Adventitious root; LkWOX4; RNA-Seq; Interaction protein
MeSH headings : Abscisic Acid / metabolism; Cloning, Molecular; Cyclopentanes / metabolism; Gene Expression Regulation, Developmental / genetics; Gene Expression Regulation, Plant / genetics; Homeodomain Proteins / genetics; Indoleacetic Acids / metabolism; Larix / genetics; Larix / growth & development; Meristem / genetics; Meristem / growth & development; Organogenesis, Plant / genetics; Oxylipins / metabolism; Signal Transduction / genetics; Transcription Factors / genetics
TL;DR: Cloning the WOX4 from the AR of Larix kaempferi found LkWOX4 involves in the AR initiation and development, which might be regulated through the IAA, JA and ABA signaling pathways. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: September 21, 2020

2019 journal article

Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Panicum virgatum L.)

Biotechnology for Biofuels, 12(1).

By: C. Ondzighi-Assoume*, J. Willis*, W. Ouma*, S. Allen*, Z. King*, W. Parrott*, W. Liu*, J. Burris*, S. Lenaghan*, C. Stewart*

author keywords: Agrobacterium tumefaciens; Bioenergy; Cell suspension culture; Genetic engineering; Switchgrass; Transformation
TL;DR: A new cell suspension-based protocol enables high-throughput Agrobacterium-mediated transformation and regeneration of switchgrass in which plants are recovered within 6–7 months from culture establishment. (via Semantic Scholar)
Source: ORCID
Added: January 6, 2021

2019 journal article

Transcription Coactivator ANGUSTIFOLIA3 (AN3) Regulates Leafy Head Formation in Chinese Cabbage

Frontiers in Plant Science.

Wusheng Liu

author keywords: AN3; BRM; leafy head formation; VIGS; transcriptome analysis; Chinese cabbage
TL;DR: This study analyzed the expression patterns of the Chinese cabbage AN3 gene (BrAN3) before and after leafy head formation, and produced BrAN3 gene silencing plants by using the turnip yellow mosaic virus (TYMV)-derived vector in order to explore its potential function in leafyHead formation in Chinese cabbage. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: ORCID
Added: May 13, 2019

2018 journal article

A profilin gene promoter from switchgrass (Panicum virgatum L.) directs strong and specific transgene expression to vascular bundles in rice

PLANT CELL REPORTS, 37(4), 587–597.

By: W. Xu*, W. Liu*, R. Ye*, M. Mazarei*, D. Huang*, X. Zhang*, C. Stewart*

Contributors: W. Xu*, W. Liu*, R. Ye*, M. Mazarei*, D. Huang*, X. Zhang*, C. Stewart*

author keywords: PvPfn2; Vascular bundle-specific promoter; Serial promoter deletion; Stable rice transformation; Switchgrass; Rice
MeSH headings : Amino Acid Sequence; Flowers / genetics; Flowers / metabolism; Gene Expression Profiling; Gene Expression Regulation, Plant; Glucuronidase / genetics; Glucuronidase / metabolism; Oryza / genetics; Oryza / metabolism; Panicum / genetics; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Roots / genetics; Plant Roots / metabolism; Plant Vascular Bundle / genetics; Plant Vascular Bundle / metabolism; Plants, Genetically Modified; Profilins / genetics; Promoter Regions, Genetic / genetics; Sequence Homology, Amino Acid; Transgenes / genetics
TL;DR: Results indicated that the PvPfn2 promoter variants are new tools to direct transgene expression specifically to vascular tissues in monocots, especially in roots, nodes and inflorescences. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2018 journal article

Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass

BIOTECHNOLOGY FOR BIOFUELS, 11.

By: W. Liu*, M. Mazarei*, R. Ye*, Y. Peng*, Y. Shao*, H. Baxter*, R. Sykes*, G. Turner* ...

author keywords: Green tissue-specific promoter; PvLhcb; PvPEPC; PvPsbR; PvMYB4; Transgenic rice; Transgenic switchgrass
TL;DR: Three switchgrass green tissue-specific promoters are identified and functionally characterized and used to direct the expression of the switchgrass MYB4 (PvMYB4) transcription factor gene in transgenic switchgrass to endow reduced recalcitrance in aboveground biomass and show utility to change cell wall biosynthesis exclusively inAboveground harvestable biomass without altering root systems. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Synthetic promoters for precise control of gene expression in plants

Chemical Engineering Progress, 113, 36–39.

By: C. Stewart & W. Liu

Source: NC State University Libraries
Added: July 28, 2019

2016 journal article

Field Studies on Dynamic Pollen Production, Deposition, and Dispersion of Glyphosate-Resistant Horseweed (Conyza canadensis)

Weed Science, 64(1), 101–111.

By: R. Ye*, H. Huang*, J. Alexander*, W. Liu*, R. Millwood*, J. Wang*, C. Stewart*

Contributors: R. Ye*, H. Huang*, J. Alexander*, W. Liu*, R. Millwood*, J. Wang*, C. Stewart*

author keywords: Pollen release rate; pollen release pattern; pollen dispersion pattern
TL;DR: Glyphosate-resistant horseweed has become an especially problematic weed in different crop production systems across the United States and the world and the first result of a systematic, direct examination of the release rate (emission and deposition), release pattern (daily and seasonal), and dispersion pattern of GR horseweed pollen is found. (via Semantic Scholar)
Source: ORCID
Added: May 13, 2019

2016 journal article

Plant synthetic promoters and transcription factors

Current Opinion in Biotechnology, 37, 36–44.

By: W. Liu* & C. Stewart*

Contributors: W. Liu* & C. Stewart*

MeSH headings : Humans; Metabolic Engineering; Plants / genetics; Plants / metabolism; Promoter Regions, Genetic; Transcription Factors / genetics; Transcription Factors / metabolism; Transgenes
TL;DR: The recent advances in synthetic promoters and TFs in plants are reviewed and a speculate on their future is speculated. (via Semantic Scholar)
Source: ORCID
Added: May 13, 2019

2016 chapter

Plant systems biology

In C. N. Stewart Jr (Ed.), Plant Biotechnology and Genetics: Principles, Techniques and Applications (2nd edition). New Jersey, USA: Wiley and Sons.

By: W. Liu & C. Stewart

Ed(s): C. Stewart

Source: NC State University Libraries
Added: July 28, 2019

2016 chapter

Promoters and Marker genes

In C. N. Stewart Jr (Ed.), Plant Biotechnology and Genetics: Principles, Techniques and Applications (2nd edition). New Jersey, USA: Wiley and Sons.

By: W. Liu, B. Miki & C. Stewart

Ed(s): C. Stewart

Source: NC State University Libraries
Added: July 28, 2019

2016 chapter

The Future: advanced plant biotechnology, genome editing and synthetic biology

In C. N. Stewart Jr (Ed.), Plant Biotechnology and Genetics: Principles, Techniques and Applications (2nd edition). New Jersey, USA: Wiley and Sons.

By: W. Liu & C. Stewart

Ed(s): C. Stewart

Source: NC State University Libraries
Added: July 28, 2019

2015 journal article

Plant synthetic biology

Trends in Plant Science, 20(5), 309–317.

By: W. Liu* & C. Stewart*

Contributors: W. Liu* & C. Stewart*

author keywords: plant synthetic biology; design cycle; enabling tools; engineering principles; pioneering applications
MeSH headings : Genetic Engineering / methods; Plants / metabolism; Synthetic Biology / methods
TL;DR: The design cycles of synthetic biology as well as key engineering principles, genetic parts, and computational tools that can be utilized in plant synthetic biology are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: ORCID
Added: May 13, 2019

2014 journal article

Computational discovery of soybean promoter cis-regulatory elements for the construction of soybean cyst nematode-inducible synthetic promoters

Plant Biotechnology Journal, 12(8), 1015–1026.

By: W. Liu*, M. Mazarei*, Y. Peng*, M. Fethe*, M. Rudis*, J. Lin*, R. Millwood*, P. Arelli, C. Stewart*

Contributors: W. Liu*, M. Mazarei*, Y. Peng*, M. Fethe*, M. Rudis*, J. Lin*, R. Millwood*, P. Arelli, C. Stewart*

author keywords: de novo motif discovery; plant synthetic biology; pporRFP; transgenic soybean hairy system; soybean cyst nematode; soybean
MeSH headings : Amino Acid Motifs; Animals; Biotechnology; Computational Biology; Crops, Agricultural; Gene Expression Regulation, Plant; Plant Proteins / genetics; Plant Roots / genetics; Plant Roots / parasitology; Plants, Genetically Modified; Promoter Regions, Genetic / genetics; Regulatory Sequences, Nucleic Acid / genetics; Soybeans / genetics; Soybeans / parasitology; Synthetic Biology; Tylenchida / genetics; Tylenchida / physiology
TL;DR: An ensemble strategy for de novo soybean cyst nematode (SCN)-inducible motif discovery among promoters of 18 co-expressed soybean genes that were selected from six reported microarray studies involving a compatible soybean-SCN interaction offers great potential for novel motif discovery and synthetic promoter engineering for any plant and trait in crop biotechnology. (via Semantic Scholar)
Source: ORCID
Added: May 13, 2019

2014 journal article

Synthetic TAL effectors for targeted enhancement of transgene expression in plants

Plant Biotechnology Journal, 12(4), 436–446.

By: W. Liu*, M. Rudis*, Y. Peng*, M. Mazarei*, R. Millwood*, J. Yang*, W. Xu*, J. Chesnut*, C. Stewart*

Contributors: W. Liu*, M. Rudis*, Y. Peng*, M. Mazarei*, R. Millwood*, J. Yang*, W. Xu*, J. Chesnut*, C. Stewart*

author keywords: targeted transgene activation; synthetic TALEs; additive effects; pporRFP; AtPAP1; transgenic plants
MeSH headings : Arabidopsis Proteins / metabolism; Base Sequence; Binding Sites; DNA, Plant / metabolism; Gene Expression Regulation, Plant; Genes, Reporter; Genetic Engineering / methods; Pigmentation; Plant Leaves / physiology; Plants, Genetically Modified; Promoter Regions, Genetic / genetics; Protein Binding; Protein Structure, Tertiary; Tobacco / genetics; Trans-Activators / chemistry; Trans-Activators / metabolism; Transcription Factors / metabolism; Transcriptional Activation; Transgenes / genetics
TL;DR: It is demonstrated that transient expression of de novo-engineered TALEs using agroinfiltration could significantly increase reporter gene expression in stable transgenic tobacco expressing the orange fluorescent protein reporter gene pporRFP under the control of synthetic inducible, minimal or full-length 35S promoters. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: May 13, 2019

2014 journal article

The performance of pathogenic bacterial phytosensing transgenic tobacco in the field

Plant Biotechnology Journal, 12(6), 755–764.

By: M. Fethe*, W. Liu*, J. Burris*, R. Millwood*, M. Mazarei*, M. Rudis*, D. Yeaman*, M. Dubosquielle*, C. Stewart*

Contributors: M. Fethe*, W. Liu*, J. Burris*, R. Millwood*, M. Mazarei*, M. Rudis*, D. Yeaman*, M. Dubosquielle*, C. Stewart*

author keywords: transgenic plants; phytosensor; synthetic promoters; phytopathogen sensing; phytobacterial pathogens; field tested
MeSH headings : Cyclopentanes / pharmacology; Ethylenes / pharmacology; Luminescent Proteins / metabolism; Oxylipins / pharmacology; Plant Diseases / microbiology; Plants, Genetically Modified; Promoter Regions, Genetic; Pseudomonas syringae / drug effects; Pseudomonas syringae / growth & development; Pseudomonas syringae / physiology; Salicylic Acid / pharmacology; Time Factors; Tobacco / drug effects; Tobacco / genetics; Tobacco / microbiology; Transcription, Genetic / drug effects; Transgenes
TL;DR: Transgenic tobacco harbouring a synthetic inducible promoter-driven OFP could be used to facilitate monitoring and early-warning reporting of phytopathogen infections in agricultural fields. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: May 13, 2019

2013 journal article

Advanced genetic tools for plant biotechnology

Nature Reviews Genetics, 14(11), 781–793.

Contributors: W. Liu*, J. Yuan* & C. Stewart Jr.

MeSH headings : Agriculture; Biotechnology; Chromosomes, Artificial; Crops, Agricultural / genetics; DNA Nucleotidyltransferases / genetics; DNA Nucleotidyltransferases / metabolism; Gene Transfer Techniques; Genetic Engineering / methods; Genome, Plant; Mutagenesis, Site-Directed; Plants / genetics; Plants, Genetically Modified; Promoter Regions, Genetic; Transcription Factors / genetics; Transcription Factors / metabolism
TL;DR: Advanced tools that are currently available for use in plant biotechnology to produce new products in plants and to generate plants with new functions are discussed, including synthetic promoters, 'tunable' transcription factors, genome-editing tools and site-specific recombinases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: ORCID
Added: May 13, 2019

2013 journal article

Bacterial pathogen phytosensing in transgenic tobacco and Arabidopsis plants

Plant Biotechnology Journal, 11(1), 43–52.

By: W. Liu*, M. Mazarei*, M. Rudis*, M. Fethe*, Y. Peng*, R. Millwood*, G. Schoene*, J. Burris*, C. Stewart*

Contributors: W. Liu*, M. Mazarei*, M. Rudis*, M. Fethe*, Y. Peng*, R. Millwood*, G. Schoene*, J. Burris*, C. Stewart Jr.

author keywords: Inducible regulatory element; synthetic promoter; transgenic plants; phytosensor; phytohormone; bacterial pathogen; fluorescent protein reporter
MeSH headings : Arabidopsis / genetics; Arabidopsis / microbiology; Crops, Agricultural / microbiology; Cyclopentanes / metabolism; Disease Resistance / genetics; Ethylenes / metabolism; Gene Expression Regulation, Bacterial; Genes, Plant; Genes, Reporter; Green Fluorescent Proteins / genetics; Host-Pathogen Interactions / genetics; Oxylipins / metabolism; Plant Diseases / microbiology; Plant Growth Regulators / metabolism; Plants, Genetically Modified / metabolism; Plants, Genetically Modified / microbiology; Promoter Regions, Genetic; Regulatory Elements, Transcriptional; Salicylic Acid / metabolism; Tobacco / genetics; Tobacco / microbiology; Transgenes
TL;DR: Insight is provided into the potential applications of transgenic plants as phytosensors and the implementation of emerging technologies for monitoring plant disease outbreaks in agricultural fields. (via Semantic Scholar)
Sources: ORCID, Crossref
Added: May 13, 2019

2013 journal article

Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode

Plant Biotechnology Journal, 11(9), 1135–1145.

Contributors: J. Lin*, M. Mazarei*, N. Zhao*, J. Zhu*, X. Zhuang*, W. Liu*, V. Pantalone*, P. Arelli*, C. Stewart*, F. Chen*

author keywords: methyl salicylate; transgenic hairy roots; soybean; Heterodera glycines
MeSH headings : Amino Acid Sequence; Animals; DNA, Complementary / genetics; Disease Resistance; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genotype; Kinetics; Methyltransferases / genetics; Methyltransferases / metabolism; Molecular Sequence Data; Phylogeny; Plant Diseases / immunology; Plant Diseases / parasitology; Plant Proteins / genetics; Plant Proteins / metabolism; Plant Roots / enzymology; Plant Roots / genetics; Plant Roots / immunology; Plants, Genetically Modified; Salicylic Acid / metabolism; Sequence Alignment; Signal Transduction; Soybeans / enzymology; Soybeans / genetics; Soybeans / immunology; Tylenchoidea / physiology
TL;DR: It is reported that a SAMT gene from soybean (GmSAMT1) plays a role in soybean defence against soybean cyst nematode (Heterodera glycines Ichinohe, SCN). (via Semantic Scholar)
Source: ORCID
Added: May 13, 2019

2011 journal article

Gene expression profiling of resistant and susceptible soybean lines infected with soybean cyst nematode

Theoretical and Applied Genetics, 123(7), 1193–1206.

Contributors: M. Mazarei*, W. Liu*, H. Al-Ahmad*, P. Arelli*, V. Pantalone* & C. Stewart Jr.

MeSH headings : Animals; False Positive Reactions; Gene Expression Profiling / methods; Gene Expression Regulation, Plant; Genes, Plant; Genetic Techniques; Models, Genetic; Nematoda; Oligonucleotide Array Sequence Analysis; Plant Diseases / genetics; RNA, Messenger / metabolism; Soybeans / genetics; Tylenchoidea / metabolism
TL;DR: Microarray analyses examining two genetically related soybean lines against the same SCN population provided additional insights into the specific changes in gene expression of a susceptible and a resistant reaction beneficial for identification of genes involved in defense. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: ORCID
Added: May 13, 2019

2011 journal article

Rapid in vivo analysis of synthetic promoters for plant pathogen phytosensing

BMC Biotechnology, 11.

Contributors: W. Liu*, M. Mazarei*, M. Rudis*, M. Fethe* & C. Stewart Jr

MeSH headings : Agrobacterium / genetics; Agrobacterium / metabolism; Genes, Reporter / genetics; Genetic Engineering / methods; Host-Pathogen Interactions; Luminescent Proteins / metabolism; Plant Leaves / metabolism; Plant Leaves / microbiology; Plants, Genetically Modified; Plasmids / genetics; Promoter Regions, Genetic / genetics; Pseudomonas / growth & development; Pseudomonas / pathogenicity; Tobacco / genetics; Tobacco / microbiology
TL;DR: These observations demonstrate that the Agrobacterium-mediated transient expression is an efficient method for in vivo assays of promoter constructs in less than one week, and provide the opportunity to gain further insights into the versatility of the expression system as a potential tool for high-throughput in planta expression screening. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: May 13, 2019

2011 journal article

Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation

BMC Biotechnology, 11(1).

By: D. Mann*, Z. King*, W. Liu*, B. Joyce*, R. Percifield*, J. Hawkins*, P. LaFayette*, B. Artelt* ...

MeSH headings : Genes, Plant; Genetic Techniques; Molecular Sequence Data; Oryza / genetics; Panicum / genetics; Plants, Genetically Modified; Polyubiquitin / genetics; Promoter Regions, Genetic; Tobacco / genetics; Transformation, Genetic; Transgenes
TL;DR: The PvUBI1 and PvUbi2 promoters drive expression in switchgrass, rice and tobacco and are strong constitutive promoter candidates that will be useful in genetic transformation of monocots and dicots. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 28, 2019

2011 journal article

The presence of multiple introns is essential for ERECTA expression in Arabidopsis

RNA, 17(10), 1907–1921.

Contributors: R. Karve*, W. Liu*, S. Willet*, K. Torii* & E. Shpak*

author keywords: gene expression; intron-mediated enhancement; splicing; intron; ERECTA
MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Exons; Gene Expression Regulation, Plant; Introns; Mutation; Poly A / genetics; Promoter Regions, Genetic; Protein Biosynthesis; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; RNA, Messenger / metabolism; Receptors, Cell Surface / genetics; Receptors, Cell Surface / metabolism
TL;DR: It is demonstrated that introns are essential for ERECTA mRNA accumulation and, to a lesser extent, for mRNA utilization in translation. (via Semantic Scholar)
Source: ORCID
Added: May 13, 2019

2005 journal article

The tortoise and the hare II: Relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis

American Journal of Botany, 92(1), 142–166.

By: J. Shaw*, E. Lickey*, J. Beck*, S. Farmer*, W. Liu*, J. Miller*, K. Siripun*, C. Winder*, E. Schilling*, R. Small*

Contributors: J. Shaw*, E. Lickey*, J. Beck*, S. Farmer*, W. Liu*, J. Miller*, K. Siripun*, C. Winder*, E. Schilling*, R. Small*

TL;DR: The results of this study show that a survey using as few as three representative taxa can be predictive of the amount of phylogenetic information offered by a cpDNA region and that rate heterogeneity exists among noncoding cpDNA regions. (via Semantic Scholar)
Source: ORCID
Added: May 13, 2019

1999 journal article

Wild fruit resources and exploitation in Xiaoxing’an Mountains

Journal of Forestry Research, 10(1), 31–33.

By: Z. Lihuan*, W. Ling*, C. Quanshe* & L. Wusheng*

TL;DR: The ornamental characters, nutritious composition, edible value and medical value of thirteen kinds of major wild fruit resources in Xiaoxing’an Mountains Region were synthetically analyzed to get rid of economic crisis early in this region. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, ORCID
Added: July 28, 2019

1998 journal article

Investigation on the Medical Plant Resources of Beizhang Experimental Area in the Upper Reaches of Miyun Reservoir

Quarterly of Forest By-Product And Speciality in China, 2, 45–46.

By: G. Li, Y. Zhang, J. Wang & W. Liu

Source: NC State University Libraries
Added: March 7, 2021

1997 journal article

The preliminary study on the vegetation qualities of Beizhuang experimental area in the upper reaches of Miyun reservoir

Journal of Beijing Forestry University, 19, 39–44.

By: Y. Zhang, J. Wang, W. Liu, G. Li & F. Li

Source: NC State University Libraries
Added: March 7, 2021

1994 chapter

Early spring wild herbal flower resources and its utilization in urban landscaping in Heilongjiang Province

In C. X. Yang & W. Q. Wen (Eds.), New Studies on Forest Management and Forest Resource Exploitation in Northeast China-Zhong Guo Dong Bei Lin Qu (1990-1994) (pp. 286–290). Harbin, P.R. China: Heilongjiang Science & Technology Press.

By: L. Zhuo, W. Yang & W. Liu

Ed(s): C. Yang & W. Wen

Source: NC State University Libraries
Added: March 8, 2021

1994 chapter

Investigation and utilization of the wild fruit resources in Xiaoxing’an Mountains

In C. X. Yang & W. Q. Wen (Eds.), New Studies on Forest Management and Forest Resource Exploitation in Northeast China-Zhong Guo Dong Bei Lin Qu (1990-1994) (pp. 266–270). Harbin, P.R. China: Heilongjiang Science & Technology Press.

By: L. Zhuo, W. Liu & W. Yang

Ed(s): C. Yang & W. Wen

Source: NC State University Libraries
Added: March 8, 2021

Employment

Updated: May 8th, 2019 12:40

2018 - present

North Carolina State University Raleigh, NC, US
Assistant Professor in Translational Genomics Horticultural Science

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