William Polacheck

Works (13)

Updated: April 5th, 2024 11:30

2024 article

Donor-Derived Engineered Microvessels for Cardiovascular Risk Stratification of Patients with Kidney Failure

Rathod, M. L., Aw, W. Y., Huang, S., Lu, J., Doherty, E. L., Whithworth, C. P., … Polacheck, W. J. (2024, January 7). SMALL.

By: M. Rathod n, W. Aw n, S. Huang n, J. Lu  n, E. Doherty  n, C. Whithworth*, G. Xi, P. Roy-Chaudhury *, W. Polacheck n

author keywords: blood-derived endothelial cell; cardiovascular; chronic kidney disease; indoxyl sulfate; microfluidics; organs-on-chip; uremic toxin
TL;DR: The engineered microvessels presented here have the potential to be utilized to risk stratify and to direct personalized treatments for patients with chronic kidney disease and an increase in sensitivity compared to traditional 2D assays. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: January 29, 2024

2023 journal article

Microphysiological model of PIK3CA-driven vascular malformations reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation

SCIENCE ADVANCES, 9(7).

By: W. Aw  n, C. Cho  n, H. Wang  n, A. Cooper n, E. Doherty  n, D. Rocco *, S. Huang n, S. Kubik n ...

MeSH headings : Humans; TOR Serine-Threonine Kinases / metabolism; Mechanistic Target of Rapamycin Complex 1 / metabolism; Sirolimus / pharmacology; Human Umbilical Vein Endothelial Cells / metabolism; Mitogen-Activated Protein Kinase Kinases / metabolism; Class I Phosphatidylinositol 3-Kinases / genetics; Class I Phosphatidylinositol 3-Kinases / metabolism; Vascular Malformations / metabolism; rac1 GTP-Binding Protein / metabolism
TL;DR: These findings demonstrate an in vitro platform for VMs and establish a role of dysregulated Rac1/PAK and mTORC1/2 signaling in PIK3CA-driven VMs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: March 27, 2023

2023 journal article

Patient-derived extracellular matrix demonstrates role of COL3A1 in blood vessel mechanics

ACTA BIOMATERIALIA, 166, 346–359.

By: E. Doherty  n, W. Aw n, E. Warren  n, M. Hockenberry *, C. Whitworth *, G. Krohn  n, S. Howell *, B. Diekman n ...

author keywords: Vascular Ehlers-Danlos syndrome; Cell derived matrix; Collagen III; Mechanotransduction; ECM mechanics; Viscoelasticity
TL;DR: This work generates fibrous, collagen-rich ECM from primary donor cells from patients with vascular Ehlers-Danlos syndrome, a disease caused by mutations in the gene that encodes collagen III, and observes that ECM grown from vEDS patients is characterized by unique mechanical signatures, including altered viscoelastic properties. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: August 7, 2023

2023 article

Vascular organs-on-chip made with patient-derived endothelial cells: technologies to transform drug discovery and disease modeling

Whitworth, C. P., & Polacheck, W. J. (2023, December 22). EXPERT OPINION ON DRUG DISCOVERY.

By: C. Whitworth * & W. Polacheck n

author keywords: Blood outgrowth endothelial cell; induced pluripotent stem cells; disease modeling; drug discovery; endothelial colony forming cell; organ-on-chip; Vascular disease
TL;DR: The authors review induced pluripotent stem cells and blood outgrowth endothelial cells as two sources for patient-derived endothelial cells (EC) as two sources for patient-derived endothelial cells (EC) with a focus on applications for drug discovery. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: January 8, 2024

2022 journal article

A vascularized model of the human liver mimics regenerative responses

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(28).

By: A. Chhabra*, H. Song*, K. Grzelak*, W. Polacheck n, H. Fleming *, C. Chen *, S. Bhatia *

author keywords: hepatocyte; regeneration; vascular
MeSH headings : Cell Culture Techniques, Three Dimensional; Cytokines; Endothelial Cells; Hepatocytes; Humans; Liver / blood supply; Liver Regeneration / physiology
TL;DR: A microfluidic device is developed that allows us to manipulate fluid flow, circulating cytokines, and/or paracrine interactions between liver and vascular cells, in order to model multicellular aspects of human liver regeneration and found that physiologically relevant shear stresses increased the secretion of angiogenesis- and regeneration-associated factors. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: September 29, 2022

2022 journal article

Multilayer microfluidic platform for the study of luminal, transmural, and interstitial flow

BIOFABRICATION, 14(2).

By: G. Lee n, S. Huang n, W. Aw  n, M. Rathod  n, C. Cho  n, F. Ligler n, W. Polacheck n

author keywords: microfluidics; vascular biology; interstitial flow; hemodynamics; biofluid mechanics; mechanotransduction
MeSH headings : Lymphatic Vessels; Microfluidics / methods; Stress, Mechanical
TL;DR: A multi-layer microfluidic platform (MμLTI-Flow) is introduced that enables the culture of engineered blood and lymphatic microvessels and independent control of blood, lymphatic, and IFPs and will provide for analysis of key fluid mechanical parameters and cellular mechanisms that contribute to diseases in which fluid imbalances play a role in progression, including lymphedema and solid cancer. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: January 31, 2022

2021 journal article

Direct comparison of angiogenesis in natural and synthetic biomaterials reveals that matrix porosity regulates endothelial cell invasion speed and sprout diameter

ACTA BIOMATERIALIA, 135, 260–273.

By: W. Wang *, R. Kent *, S. Huang n, E. Jarman*, E. Shikanov*, C. Davidson*, H. Hiraki *, D. Lin* ...

author keywords: Angiogenesis; Cell migration; Cell proliferation; Microfluidics; ECM; Endothelial cells; Chemotaxis; Hydrogels; Microvasculature; Sprouting Morphogenesis; Matrix porosity
MeSH headings : Animals; Biocompatible Materials / pharmacology; Endothelial Cells; Extracellular Matrix; Hydrogels / pharmacology; Mice; Neovascularization, Physiologic; Porosity
TL;DR: Characterization of matrix properties and sprout morphometrics across these materials points to matrix porosity as a critical regulator of sprout invasion speed and diameter, supported by the observation that nanoporous DexVS hydrogels yielded endothelial cell sprouts that were not perfusable. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: November 29, 2021

2021 review

Malignant Ascites in Ovarian Cancer: Cellular, Acellular, and Biophysical Determinants of Molecular Characteristics and Therapy Response

[Review of ]. CANCERS, 13(17).

By: B. Rickard  n, C. Conrad*, A. Sorrin*, M. Ruhi  n, J. Reader *, S. Huang n, W. Franco *, G. Scarcelli * ...

author keywords: malignant ascites; epithelial ovarian cancer; chemoresistance; mechanical stress; flow-induced shear stress; photodynamic therapy; tumor microenvironment; transcoelomic metastases; tumor heterogeneity; epithelial to mesenchymal transition
TL;DR: The current understanding of malignant ascites is summarized, starting with an overview of ovarian cancer and ascites, followed by a description of the tools used to analyze the components of ascites and how these components modulate ovarian cancer biology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: September 20, 2021

2021 journal article

Microfluidic model of monocyte extravasation reveals the role of hemodynamics and subendothelial matrix mechanics in regulating endothelial integrity

BIOMICROFLUIDICS, 15(5).

By: S. Perez-Rodriguez n, S. Huang n, C. Borau  n, J. Manuel Garcia-Aznar & W. Polacheck n

TL;DR: A microfluidic approach is presented that enables the recreation of a three-dimensional, perfused endothelial vessel formed by human endothelial cells embedded within a collagen-rich matrix and demonstrates that extravasation of monocytes is highly regulated by the structural integrity of the endothelial monolayer. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: October 4, 2021

2020 journal article

A Bile Duct-on-a-Chip With Organ-Level Functions

HEPATOLOGY, 71(4), 1350–1363.

By: Y. Du *, G. Khandekar*, J. Llewellyn*, W. Polacheck n, C. Chen * & R. Wells *

MeSH headings : Animals; Bile Ducts / physiopathology; Cell Line; Epithelial Cells; Lab-On-A-Chip Devices; Mice; Mice, Inbred BALB C; Models, Animal
TL;DR: This bile duct-on-a-chip represents a novel in vitro platform to study the pathophysiology of the biliary duct using cholangiocytes from a variety of sources and captures essential features of a simplified biles duct in structure and organ-level functions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: November 11, 2019

2020 journal article

Functional angiogenesis requires microenvironmental cues balancing endothelial cell migration and proliferation

LAB ON A CHIP, 20(6), 1153–1166.

By: W. Wang *, D. Lin*, E. Jarman*, W. Polacheck n & B. Baker *

MeSH headings : Cell Movement; Cell Proliferation; Cues; Endothelial Cells; Humans; Neovascularization, Pathologic; Neovascularization, Physiologic
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: April 20, 2020

2020 journal article

Microfluidics for the study of mechanotransduction

JOURNAL OF PHYSICS D-APPLIED PHYSICS, 53(22).

By: C. Griffith  n, S. Huang n, C. Cho  n, T. Khare  n, M. Rich  n, G. Lee*, F. Ligler n, B. Diekman n, W. Polacheck n

author keywords: microfluidics; mechanotransduction; mechanobiology; microfabrication; cell adhesion; biofluids; microdevices
TL;DR: An overview of the main mechanotransduction pathways that function at sites of cell adhesion are given, and examples of how microfluidics can be used to apply both solid forces and fluid forces to cells are presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: April 20, 2020

2019 journal article

Microfabricated blood vessels for modeling the vascular transport barrier

NATURE PROTOCOLS, 14(5), 1425–1454.

By: W. Polacheck n, M. Kutys *, J. Tefft * & C. Chen *

MeSH headings : Capillary Permeability / physiology; Cell Culture Techniques / instrumentation; Cells, Cultured; Endothelial Cells / cytology; Equipment Design; Humans; Microfluidic Analytical Techniques / instrumentation; Microvessels / cytology; Microvessels / physiology; Models, Cardiovascular; Stromal Cells / cytology; Tissue Engineering / instrumentation
TL;DR: This protocol describes how to design and fabricate the silicon hEMV device master molds and apply calibrated fluid shear stress to hEMVs, and how to assess vascular barrier function and perform immunofluorescence imaging. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: June 4, 2019

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