Yev Brudno

Works (34)

Updated: February 10th, 2025 16:40

2024 journal article

A biomaterial platform for T cell-specific gene delivery

ACTA BIOMATERIALIA, 177, 157–164.

By: S. Pandit n, B. Smith *, M. Birnbaum * & Y. Brudno n 

author keywords: CAR T cell therapy; Pseudotyped lentivirus; Targeting; T cell activation; Transduction; Alginate; Macroporous scaffolds; T cell engineering; Gene delivery
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Sources: Web Of Science, NC State University Libraries
Added: May 28, 2024

2024 journal article

Implantable CAR T cell factories enhance solid tumor treatment

BIOMATERIALS, 308.

By: S. Pandit n, P. Agarwalla n, F. Song *, A. Jansson*, G. Dotti* & Y. Brudno n 

author keywords: Biomaterials; CAR T cell; immunotherapy; Cell therapy; implant; porous matrix
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Sources: Web Of Science, NC State University Libraries
Added: August 26, 2024

2024 journal article

Toxicology study of a tissue anchoring paclitaxel prodrug

BMC PHARMACOLOGY & TOXICOLOGY, 25(1).

By: R. Chinthapatla n, J. Stephens n, I. Neumann-Rivera n, N. Henderson n, M. Nie n, H. Haynes  n, J. Pierce n , D. Meritet n, Y. Brudno n , A. Oh n 

author keywords: Tissue-Reactive Anchoring Pharmaceuticals (TRAPs); Paclitaxel; Drug delivery; Cremophor; Local chemotherapy
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Sources: Web Of Science, NC State University Libraries
Added: December 16, 2024

2023 article

Absorption rate governs cell transduction in dry macroporous scaffolds

VanBlunk, M., Srikanth, V., Pandit, S. S., Kuznetsov, A. V., & Brudno, Y. (2023, January 26). BIOMATERIALS SCIENCE, Vol. 1.

By: M. VanBlunk n, V. Srikanth n, S. Pandit n, A. Kuznetsov n & Y. Brudno n 

MeSH headings : Tissue Scaffolds; Biocompatible Materials; Cell Differentiation; Porosity; Tissue Engineering / methods
TL;DR: The rate of liquid flow through the scaffolds, rather than pore size or stiffness, serves as a central regulator for efficient Drydux transduction, which provides a fast and inexpensive method for transducing cells for cellular therapy, including for the production of CAR T cells. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: March 13, 2023

2023 journal article

Loading Intracranial Drug-Eluting Reservoirs Across the Blood-Brain Barrier With Focused Ultrasound

ULTRASOUND IN MEDICINE AND BIOLOGY, 49(7), 1679–1685.

By: C. Moody n, P. Durham  n, P. Dayton n & Y. Brudno n 

author keywords: Blood-brain barrier; Brain delivery; Click chemistry; Drug delivery; Focused ultrasound
MeSH headings : Female; Mice; Animals; Blood-Brain Barrier; Drug Delivery Systems / methods; Neurodegenerative Diseases; Brain / diagnostic imaging; Microbubbles; Magnetic Resonance Imaging / methods
TL;DR: The ability to target and retain small molecules at predetermined intracranial sites with pinpoint accuracy provides opportunities to continuously deliver drugs to the brain over weeks and months without excessive BBB opening and with minimal off-target side effects. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 19, 2023

2022 article

Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells

Agarwalla, P., Ogunnaike, E. A., Ahn, S., Froehlich, K. A., Jansson, A., Ligler, F. S., … Brudno, Y. (2022, March 24). NATURE BIOTECHNOLOGY, Vol. 3.

By: P. Agarwalla n, E. Ogunnaike *, S. Ahn*, K. Froehlich n, A. Jansson n, F. Ligler n, G. Dotti*, Y. Brudno n 

MeSH headings : Animals; Antigens, CD19; B-Lymphocytes; Humans; Immunotherapy, Adoptive / methods; Leukocytes, Mononuclear / metabolism; Mice; Receptors, Antigen, T-Cell; T-Lymphocytes
TL;DR: An implantable Multifunctional Alginate Scaffold for T Cell Engineering and Release (MASTER) that streamlines in vivo CAR-T cell manufacturing and reduces processing time to a single day is described. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: April 18, 2022

2022 journal article

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 9(187).

By: M. VanBlunk n, P. Agarwalla n, S. Pandit n & Y. Brudno n 

MeSH headings : Alginates; Calcium; Immunotherapy, Adoptive; Receptors, Chimeric Antigen; T-Lymphocytes; Tissue Engineering; Tissue Scaffolds
TL;DR: Dry macroporous alginate scaffolds serve as a cheaper and more convenient alternative to the conventional transduction method for CAR-T cells and are comparable to the transduction efficiency of spinoculation on retronectin-coated plates. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 30, 2023

2022 journal article

Tissue-reactive drugs enable materials-free local depots

JOURNAL OF CONTROLLED RELEASE, 343, 142–151.

By: S. Pandit n, S. Palvai n, N. Massaro n, J. Pierce n  & Y. Brudno n 

author keywords: Sustained release; Materials-free delivery; Paclitaxel; Pancreatic cancer; Extracellular matrix; N-hydroxysuccinimide ester
MeSH headings : Animals; Cell Line, Tumor; Drug Delivery Systems; Drug Liberation; Hydrogels; Mice; Paclitaxel; Pancreatic Neoplasms / drug therapy
TL;DR: It is demonstrated that locally injected TRAPs create dispersed, stable intratumoral depots deep within mouse and human pancreatic tumor tissues, and had better solubility than free paclitaxel and enabled sustained in vitro and in vivo drug release. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: May 2, 2022

2021 journal article

On-Demand Drug Release from Click-Refillable Drug Depots

MOLECULAR PHARMACEUTICS, 18(10), 3920–3925.

By: S. Palvai n, C. Moody n, S. Pandit n & Y. Brudno n 

author keywords: drug delivery; bioorthogonal; stimuli-responsive; photocleavage; click chemistry
MeSH headings : Animals; Antineoplastic Agents / administration & dosage; Antineoplastic Agents / pharmacokinetics; Cell Line, Tumor; Click Chemistry / methods; Delayed-Action Preparations; Doxorubicin / administration & dosage; Doxorubicin / pharmacokinetics; Drug Delivery Systems / methods; Drug Liberation; Fluorescent Dyes; Humans; Mice
TL;DR: An on- demand drug delivery paradigm that combines bioorthogonal click chemistry to locally enrich protodrugs at a prelabeled site and light-triggered drug release at the target tissue is reported, establishing a novel method for in vivo targeting and on-demand delivery of cytotoxic drugs at target tissues. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 26, 2021

2021 journal article

Restoring Carboxylates on Highly Modified Alginates Improves Gelation, Tissue Retention and Systemic Capture

Acta Biomaterialia, 138, 208–217.

By: C. Moody n, A. Brown n , N. Massaro n, A. Patel n, P. Agarwalla n, A. Simpson  n, A. Brown n, H. Zheng n , J. Pierce n , Y. Brudno n 

author keywords: Alginate; Click chemistry; Bioconjugation; Ionic cross-linking; Drug capture
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 31, 2021

2020 journal article

Click cross-linking improves retention and targeting of refillable alginate depots

Acta Biomaterialia, 112, 112–121.

By: C. Moody n, S. Palvai n & Y. Brudno n 

MeSH headings : Alginates; Biocompatible Materials; Click Chemistry; Cross-Linking Reagents; Hydrogels; Tissue Engineering
Source: ORCID
Added: July 8, 2023

2020 journal article

In Vivo Targeting Using Arylboronate/Nopoldiol Click Conjugation

BIOCONJUGATE CHEMISTRY, 31(10), 2288–2292.

By: S. Palvai n, J. Bhangu*, B. Akgun*, C. Moody n, D. Hall * & Y. Brudno n 

MeSH headings : Animals; Boronic Acids / chemical synthesis; Boronic Acids / chemistry; Click Chemistry / methods; Crystallography, X-Ray; Magnetic Resonance Spectroscopy; Mice; Models, Molecular; Semicarbazides / chemical synthesis; Semicarbazides / chemistry
TL;DR: A detailed structural analysis of the arylboronate/nopoldiol adduct is reported that the bioorthogonal reactants form, unexpectedly, a tetracyclic adduct through the cyclization of the distal nitrogen into the semithiocarbazone leading to a strong B-N dative bond and two new 5-membered rings. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 21, 2020

2020 journal article

Regenerating Antithrombotic Surfaces through Nucleic Acid Displacement

ACS BIOMATERIALS SCIENCE & ENGINEERING, 6(4), 2159–2166.

By: S. McNamara *, Y. Brudno n , A. Miller *, H. Ham*, M. Aizenberg, E. Chaikof *, D. Mooney *

author keywords: blood-contacting devices; antithrombotic; refillable surface coatings; oligonucleotides; toehold exchange
MeSH headings : Fibrin; Fibrinogen; Fibrinolytic Agents / pharmacology; Nucleic Acids; Regeneration
TL;DR: This study presents a unique strategy for achieving a continuous antithrombotic state for blood-contacting devices using an ODN-based regeneration method using oligodeoxynucleotide toehold exchange. (via Semantic Scholar)
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Added: May 8, 2020

2020 journal article

Scaffold-Mediated Static Transduction of T Cells for CAR-T Cell Therapy

ADVANCED HEALTHCARE MATERIALS, 9(14).

By: P. Agarwalla n, E. Ogunnaike *, S. Ahn*, F. Ligler n, G. Dotti * & Y. Brudno n 

author keywords: alginate scaffold; CAR-T cells; cell therapy; immunotherapy; viral transduction
MeSH headings : Antigens, CD19; Cell- and Tissue-Based Therapy; Humans; Immunotherapy, Adoptive; Receptors, Chimeric Antigen / genetics; T-Lymphocytes
TL;DR: The data show that macroporous alginate scaffolds serve as an attractive alternative to current transduction protocols and have high potential for clinical translation to genetically modify T cells for adoptive cellular therapy. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: June 29, 2020

2019 journal article

Biomimetics: Platelet-Inspired Nanocells for Targeted Heart Repair After Ischemia/Reperfusion Injury (Adv. Funct. Mater. 4/2019)

Advanced Functional Materials, 29(4), 1970019.

By: T. Su  n, K. Huang  n, H. Ma *, H. Liang  n, P. Dinh  n, J. Chen  n, D. Shen  n, T. Allen  n ...

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Sources: Crossref, NC State University Libraries
Added: February 21, 2020

2019 journal article

Clickable, acid labile immunosuppressive prodrugs for in vivo targeting

Biomaterials Science, 8(1), 266–277.

By: H. Wang *, M. Sobral*, T. Snyder, Y. Brudno* , V. Gorantla * & D. Mooney *

TL;DR: Dibenzocyclooctyne-modified prodrugs of the immunosuppressive drugs tacrolimus, rapamycin and mycophenolic acid are developed and targeted conjugation both in vitro and in vivo to azido-modified hydrogels via Click chemistry is demonstrated. (via Semantic Scholar)
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Source: ORCID
Added: July 8, 2023

2019 journal article

Extracellular-Matrix-Anchored Click Motifs for Specific Tissue Targeting

Molecular Pharmaceutics, 17(2), 392–403.

By: M. Adams n, C. Moody n, J. Sollinger n & Y. Brudno n 

author keywords: drug delivery; click chemistry; pancreatic cancer; brain delivery; extracellular matrix; computer modeling
MeSH headings : Animals; Antineoplastic Agents / administration & dosage; Antineoplastic Agents / pharmacokinetics; Brain / metabolism; Cell Line, Tumor; Click Chemistry / methods; Computational Biology / methods; Computer Simulation; Disease Models, Animal; Drug Delivery Systems / methods; Esters / administration & dosage; Esters / chemistry; Esters / pharmacokinetics; Extracellular Matrix / drug effects; Female; Hydrogels / administration & dosage; Mice; Mice, Inbred C57BL; Pancreatic Neoplasms / drug therapy; Pancreatic Neoplasms / pathology; Succinimides; Tissue Distribution
TL;DR: ECM-anchored click groups do not elicit any noticeable local or systemic toxicity or immune response and specifically capture systemically-circulating molecules at intradermal, intratumoral, and intracranial sites for multiple months. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: April 20, 2020

2018 journal article

Platelet-Inspired Nanocells for Targeted Heart Repair After Ischemia/Reperfusion Injury

ADVANCED FUNCTIONAL MATERIALS, 29(4).

By: T. Su n, K. Huang n , H. Ma *, H. Liang n, P. Dinh n, J. Chen n, D. Shen n, T. Allen  n ...

author keywords: biomimetics; drug delivery; ischemic injury; platelets; stromal cell secretome
TL;DR: A platelet‐inspired nanocell (PINC) that incorporates both prostaglandin E2 (PGE2)‐modified platelet membrane and cardiac stromal cell‐secreted factors to target the heart after I/R injury is introduced, which represents a promising therapeutic delivery platform for treating myocardial ischemia/reperfusion injury. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: February 4, 2019

2018 journal article

Replenishable drug depot to combat post-resection cancer recurrence

BIOMATERIALS, 178, 373–382.

By: Y. Brudno n , M. Pezone, T. Snyder, O. Uzun, C. Moody n, M. Aizenberg, D. Mooney *

author keywords: Biomaterials; Drug delivery; Cancer therapy; Drug depot; Sustained release
MeSH headings : Alginates / chemistry; Animals; Antineoplastic Agents / chemistry; Antineoplastic Agents / pharmacology; Antineoplastic Agents / therapeutic use; Cell Line, Tumor; Cell Proliferation / drug effects; Click Chemistry; Doxorubicin / chemistry; Doxorubicin / pharmacology; Doxorubicin / therapeutic use; Female; Hydrogels / chemistry; Kinetics; Mice; Neoplasm Recurrence, Local / drug therapy; Neoplasm Recurrence, Local / pathology; Neoplasms / pathology; Neoplasms / surgery; Prodrugs / chemical synthesis; Prodrugs / pharmacology; Prodrugs / therapeutic use; Prodrugs / toxicity; Subcutaneous Tissue / drug effects
TL;DR: A refillable hydrogel depot system based on bioorthogonal click chemistry that captures prodrug refills from the blood and subsequently releases active drugs locally in a sustained manner to improve cancer therapy while eliminating systemic side effects is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 19, 2018

2016 journal article

Improving wound healing through the use of tetrazine-modified alginate

The FASEB Journal, 30(Supplement 1), 1177.10–1177.10.

By: E. De Leon Peralta*, Y. Brudno* , B. Kwee * & D. Mooney *

Event: Experimental Biology 2016 Meeting

TL;DR: The hypothesis is that tetrazine acts directly on endothelial cells to induce angiogenesis and also activates immune cells that are proangiogenic, which may lead to a novel method for treating people with ischemic diseases. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: July 22, 2023

2015 journal article

In Vivo Targeting through Click Chemistry

ChemMedChem, 10(4), 617–620.

By: Y. Brudno* , R. Desai*, B. Kwee *, N. Joshi *, M. Aizenberg * & D. Mooney *

author keywords: bioorthogonal chemistry; click chemistry; drug delivery; drug targeting; gels
MeSH headings : Alginates / chemistry; Alkynes / administration & dosage; Alkynes / chemistry; Animals; Azides; Benzene Derivatives / administration & dosage; Benzene Derivatives / chemistry; Click Chemistry / methods; Cyclooctanes / administration & dosage; Cyclooctanes / chemistry; Drug Delivery Systems / methods; Fluorescent Dyes / chemistry; Glucuronic Acid / chemistry; Heterocyclic Compounds, 1-Ring / chemistry; Hexuronic Acids / chemistry; Hydrogels / chemistry; Mice; Muscles / metabolism
TL;DR: It is demonstrated that bioorthogonal click chemistry can be used to target circulating small molecules to hydrogels resident intramuscularly in diseased tissues and that small molecules can be repeatedly targeted to the diseased area over the course of at least one month. (via Semantic Scholar)
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Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2015 journal article

On-demand drug delivery from local depots

Journal of Controlled Release, 219, 8–17.

By: Y. Brudno*  & D. Mooney *

author keywords: Drug delivery; Depot; Polymer; Therapeutic; On-demand; Ultrasound; Magnetic; Electric; Light; Pharmaceutical; Nucleic acid; Stimulated
MeSH headings : Delayed-Action Preparations / administration & dosage; Delayed-Action Preparations / chemistry; Drug Delivery Systems; Humans; Pharmaceutical Preparations / administration & dosage; Pharmaceutical Preparations / chemistry; Polymers / administration & dosage; Polymers / chemistry
TL;DR: This review highlights recent advances in the development of polymeric systems engineered for releasing therapeutic molecules through physical and pharmacological stimulation. (via Semantic Scholar)
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Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2014 journal article

Comparison of biomaterial delivery vehicles for improving acute retention of stem cells in the infarcted heart

Biomaterials, 35(25), 6850–6858.

By: E. Roche *, C. Hastings*, S. Lewin, D. Shvartsman *, Y. Brudno* , N. Vasilyev *, F. O'Brien *, C. Walsh * ...

author keywords: Alginate; Collagen; Cell viability; Heart; Hydrogel
MeSH headings : Alginates / chemistry; Animals; Biocompatible Materials / chemistry; Cell Survival / drug effects; Cells, Cultured; Cells, Immobilized; Chitosan / chemistry; Collagen / chemistry; Female; Glucuronic Acid / chemistry; Glycerophosphates / chemistry; Hexuronic Acids / chemistry; Humans; Hydrogels / chemistry; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells / cytology; Myocardial Infarction / blood; Myocardial Infarction / therapy; Rats; Rats, Sprague-Dawley; Tissue Engineering
TL;DR: All four biomaterials were demonstrated to more efficiently deliver and retain cells when compared to a saline control, and biomaterial-based delivery approaches show promise for future development of efficient in vivo delivery techniques. (via Semantic Scholar)
Sources: Crossref, ORCID, NC State University Libraries
Added: August 28, 2020

2014 article proceedings

Pro-angiogenic factors enhance pericyte function during angiogenesis

Presented at the 2014 40th Annual Northeast Bioengineering Conference (NEBEC).

By: Y. Brudno* , M. Aizenberg * & D. Mooney *

Event: 2014 40th Annual Northeast Bioengineering Conference (NEBEC)

TL;DR: The results demonstrate the importance of temporal control over signaling in promoting vascular growth and vessel maturation and could be useful to promote healing after acute wounds and in chronic conditions such as diabetic ulcers and peripheral artery disease. (via Semantic Scholar)
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Sources: Crossref, NC State University Libraries
Added: July 9, 2023

2014 journal article

Refilling drug delivery depots through the blood

Proceedings of the National Academy of Sciences, 111(35), 12722–12727.

By: Y. Brudno* , E. Silva *, C. Kearney *, S. Lewin*, A. Miller *, K. Martinick*, M. Aizenberg *, D. Mooney *

author keywords: nanoparticle; targeting; DNA nanotechnology; controlled release; biomaterials
MeSH headings : Alginates / pharmacokinetics; Animals; Antibiotics, Antineoplastic / blood; Antibiotics, Antineoplastic / pharmacokinetics; Breast Neoplasms / drug therapy; Disease Models, Animal; Doxorubicin / blood; Doxorubicin / pharmacokinetics; Drug Delivery Systems / methods; Glucuronic Acid / blood; Glucuronic Acid / pharmacokinetics; Hexuronic Acids / blood; Hexuronic Acids / pharmacokinetics; Humans; Hydrazones / blood; Hydrazones / pharmacokinetics; Hydrogels / pharmacokinetics; Injections, Intralesional; Injections, Intravenous; Melanoma, Experimental / drug therapy; Mice; Mice, Inbred C57BL; Mice, Nude; Neoplasm Transplantation; Oligodeoxyribonucleotides / blood; Oligodeoxyribonucleotides / pharmacokinetics; Tumor Cells, Cultured; Xenograft Model Antitumor Assays
TL;DR: A new paradigm for nanotherapeutic drug delivery is suggested, and this concept is expected to have applications in refilling drug depots in cancer therapy, wound healing, and drug-eluting vascular grafts and stents. (via Semantic Scholar)
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Sources: Crossref, ORCID, NC State University Libraries
Added: August 28, 2020

2014 journal article

Sustained Delivery of VEGF Maintains Innervation and Promotes Reperfusion in Ischemic Skeletal Muscles Via NGF/GDNF Signaling

Molecular Therapy, 22(7), 1243–1253.

By: D. Shvartsman *, H. Storrie-White*, K. Lee *, C. Kearney *, Y. Brudno* , N. Ho*, C. Cezar*, C. McCann* ...

MeSH headings : Animals; Glial Cell Line-Derived Neurotrophic Factor / metabolism; Mice; Muscle, Skeletal / innervation; Nerve Growth Factor / metabolism; Nerve Regeneration / genetics; Nerve Regeneration / physiology; Signal Transduction / genetics; Signal Transduction / physiology; Vascular Endothelial Growth Factor A / administration & dosage; Vascular Endothelial Growth Factor A / therapeutic use; Wound Healing
TL;DR: It is shown that the delivery of vascular endothelial growth factor from alginate hydrogels ameliorates loss of skeletal muscle innervation after ischemic injury by promoting both maintenance and regrowth of damaged axons in mice. (via Semantic Scholar)
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Sources: Crossref, ORCID, NC State University Libraries
Added: August 28, 2020

2014 journal article

Three-Dimensional Human Tissue Models That Incorporate Diabetic Foot Ulcer-Derived Fibroblasts Mimic <i>In Vivo</i> Features of Chronic Wounds

Tissue Engineering Part C: Methods, 21(5), 499–508.

By: A. Maione*, Y. Brudno* , O. Stojadinovic*, L. Park*, A. Smith *, A. Tellechea *, E. Leal *, C. Kearney * ...

MeSH headings : Animals; Cell Culture Techniques; Cytokines / metabolism; Diabetic Foot / physiopathology; Extracellular Matrix / metabolism; Fibroblasts / cytology; Fibroblasts / metabolism; Fibroblasts / pathology; Humans; In Vitro Techniques; Keratinocytes / cytology; Male; Mice; Mice, Inbred C57BL; Neovascularization, Pathologic; Tissue Engineering / methods; Wound Healing
TL;DR: The reported panel of 3D DFU models provides a more biologically relevant platform for elucidating the cell-cell and cell-matrix-related mechanisms responsible for chronic wound pathogenesis and may improve translation of in vitro findings into efficacious clinical applications. (via Semantic Scholar)
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Sources: Crossref, NC State University Libraries
Added: July 9, 2023

2013 journal article

Enhancing microvascular formation and vessel maturation through temporal control over multiple pro-angiogenic and pro-maturation factors

Biomaterials, 34(36), 9201–9209.

By: Y. Brudno* , A. Ennett-Shepard*, R. Chen*, M. Aizenberg *, D. Mooney * & Biomaterials

author keywords: Controlled drug release; Angiogenesis; Growth factors; Drug delivery; Endothelial cell; Smooth muscle cell
MeSH headings : Angiogenesis Inducing Agents / pharmacology; Angiopoietin-1 / pharmacology; Angiopoietin-2 / pharmacology; Animals; Endothelium, Vascular / growth & development; Human Umbilical Vein Endothelial Cells / drug effects; Human Umbilical Vein Endothelial Cells / metabolism; Humans; Mice; Mice, Inbred C57BL; Microvessels / drug effects; Microvessels / growth & development; Neovascularization, Physiologic / drug effects; Pericytes / drug effects; Pericytes / metabolism; Platelet Endothelial Cell Adhesion Molecule-1 / metabolism; Platelet-Derived Growth Factor / pharmacology; Time Factors; Vascular Endothelial Growth Factor A / pharmacology
TL;DR: The results demonstrate the importance of temporal control over signaling in promoting vascular growth, vessel maturation and vascular remodeling as well as creating tissue engineered constructs and therapies aimed at promoting healing after acute wounds and in chronic conditions such as diabetic ulcers and peripheral artery disease. (via Semantic Scholar)
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Sources: Crossref, ORCID, NC State University Libraries
Added: August 28, 2020

2013 journal article

Fibroblasts Derived from Human Pluripotent Stem Cells Activate Angiogenic Responses In Vitro and In Vivo

PLoS ONE, 8(12), e83755.

By: Y. Shamis*, E. Silva *, K. Hewitt *, Y. Brudno* , S. Levenberg *, D. Mooney *, J. Garlick*

Ed(s): C. Egles

MeSH headings : Angiogenesis Inducing Agents / metabolism; Angiogenesis Inducing Agents / pharmacology; Animals; Biomarkers / metabolism; Cell Differentiation; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endothelial Cells / drug effects; Endothelial Cells / metabolism; Extremities / blood supply; Extremities / pathology; Fibroblasts / cytology; Fibroblasts / metabolism; Humans; Induced Pluripotent Stem Cells / cytology; Induced Pluripotent Stem Cells / metabolism; Ischemia / metabolism; Ischemia / pathology; Ischemia / therapy; Mice; Neovascularization, Physiologic; Pericytes / metabolism; Pluripotent Stem Cells / cytology; Pluripotent Stem Cells / metabolism; Proteome; Stem Cell Transplantation
TL;DR: It is demonstrated that stable populations of pericyte-like angiogenic cells can be generated with high efficiency from hESC and hiPSC using a directed differentiation approach, which provides new cell sources and opportunities for vascular tissue engineering and for the development of novel strategies in regenerative medicine. (via Semantic Scholar)
Source: ORCID
Added: July 8, 2023

2011 journal article

Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells

Nature, 473(7347), 394–397.

By: W. Pastor *, U. Pape *, Y. Huang *, H. Henderson *, R. Lister *, M. Ko *, E. McLoughlin*, Y. Brudno*  ...

MeSH headings : 5-Methylcytosine / analogs & derivatives; Animals; Biotinylation; Cell Line; Cytosine / analogs & derivatives; Cytosine / analysis; Cytosine / isolation & purification; Cytosine / metabolism; DNA Methylation; Embryonic Stem Cells / metabolism; Exons / genetics; Gene Expression Regulation, Developmental / genetics; Genome / genetics; Glucose / metabolism; Mice; Periodic Acid / metabolism; Promoter Regions, Genetic / genetics; Sequence Analysis, DNA / methods; Transcription Initiation Site; Transcription, Genetic / genetics
TL;DR: The results indicate that 5hmC has a probable role in transcriptional regulation, and suggest a model in which5hmC contributes to the ‘poised’ chromatin signature found at developmentally-regulated genes in ES cells. (via Semantic Scholar)
Sources: Crossref, ORCID, NC State University Libraries
Added: August 28, 2020

2009 journal article

An in vitro translation, selection and amplification system for peptide nucleic acids

Nature Chemical Biology, 6(2), 148–155.

By: Y. Brudno* , M. Birnbaum *, R. Kleiner * & D. Liu *

MeSH headings : Base Sequence; Databases, Nucleic Acid; Genetic Code; Genetic Techniques; Molecular Sequence Data; Nucleic Acid Amplification Techniques / methods; Nucleic Acid Conformation; Peptide Nucleic Acids / chemistry; Peptide Nucleic Acids / genetics
TL;DR: The development of such a system for peptide nucleic acids (PNAs) using a set of twelve PNA pentamer building blocks is reported, providing an experimental foundation for PNA evolution in the laboratory. (via Semantic Scholar)
Sources: Crossref, ORCID, NC State University Libraries
Added: October 29, 2020

2009 journal article

Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1

Science, 324(5929), 930–935.

By: M. Tahiliani *, K. Koh *, Y. Shen*, W. Pastor *, H. Bandukwala*, Y. Brudno* , S. Agarwal *, L. Iyer * ...

MeSH headings : 5-Methylcytosine / metabolism; Amino Acid Sequence; Animals; Cell Line; Cytosine / analogs & derivatives; Cytosine / analysis; Cytosine / metabolism; DNA / chemistry; DNA / metabolism; DNA Methylation; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Dinucleoside Phosphates / metabolism; Embryonic Stem Cells / chemistry; Embryonic Stem Cells / metabolism; Humans; Hydroxylation; Mass Spectrometry; Mice; Mixed Function Oxygenases; Molecular Sequence Data; Proto-Oncogene Proteins / chemistry; Proto-Oncogene Proteins / genetics; Proto-Oncogene Proteins / metabolism; RNA Interference; Sequence Alignment; Transfection
TL;DR: It is shown here that TET1, a fusion partner of the MLL gene in acute myeloid leukemia, is a 2-oxoglutarate (2OG)- and Fe(II)-dependent enzyme that catalyzes conversion of 5mC to 5-hydroxymethylcytosine (hmC) in cultured cells and in vitro. (via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: July 8, 2023

2009 journal article

Recent Progress Toward the Templated Synthesis and Directed Evolution of Sequence-Defined Synthetic Polymers

Chemistry & Biology, 16(3), 265–276.

By: Y. Brudno*  & D. Liu *

MeSH headings : Base Sequence; Directed Molecular Evolution / methods; Directed Molecular Evolution / trends; Nucleic Acids / chemical synthesis; Nucleic Acids / chemistry; Nucleotides / chemical synthesis; Nucleotides / chemistry; Phosphates / chemical synthesis; Phosphates / chemistry; Polymers / chemical synthesis; Polymers / chemistry; Ribose / chemical synthesis; Ribose / chemistry; Ribosomes / metabolism
TL;DR: This review covers recent advances in the enzymatic and nonenzymatic templated polymerization of nonnatural polymers and their potential applications in the directed evolution of sequence-defined synthetic polymers. (via Semantic Scholar)
Sources: Crossref, ORCID, NC State University Libraries
Added: August 28, 2020

2008 journal article

DNA-Templated Polymerization of Side-Chain-Functionalized Peptide Nucleic Acid Aldehydes

Journal of the American Chemical Society, 130(14), 4646–4659.

By: R. Kleiner *, Y. Brudno* , M. Birnbaum * & D. Liu *

MeSH headings : Aldehydes / chemical synthesis; Aldehydes / chemistry; DNA / chemical synthesis; DNA / chemistry; Kinetics; Magnetic Resonance Spectroscopy; Molecular Conformation; Nucleic Acid Conformation; Peptide Nucleic Acids / chemistry; Polymers / chemical synthesis; Spectrometry, Mass, Electrospray Ionization
TL;DR: The findings represent a significant step toward the evolution of sequence-defined synthetic polymers and also demonstrate that enzyme-free nucleic acid-templated polymerization can occur efficiently using substrates with a wide range of side-chain structures, functionalization positions within each building block, and functionalization densities. (via Semantic Scholar)
Sources: Crossref, ORCID, NC State University Libraries
Added: August 28, 2020

Employment

Updated: June 1st, 2021 20:14

2017 - present

University of North Carolina and North Carolina State University Raleigh, NC, US
Assistant Professor Joint Department of Biomedical Engineering

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