@article{hoque_mahmood_ali_sefat_huang_petersen_harrington_fang_gluck_2023, title={Development of a Pneumatic-Driven Fiber-Shaped Robot Scaffold for Use as a Complex 3D Dynamic Culture System}, volume={8}, ISSN={["2313-7673"]}, url={https://doi.org/10.3390/biomimetics8020170}, DOI={10.3390/biomimetics8020170}, abstractNote={Cells can sense and respond to different kinds of continuous mechanical strain in the human body. Mechanical stimulation needs to be included within the in vitro culture system to better mimic the existing complexity of in vivo biological systems. Existing commercial dynamic culture systems are generally two-dimensional (2D) which fail to mimic the three-dimensional (3D) native microenvironment. In this study, a pneumatically driven fiber robot has been developed as a platform for 3D dynamic cell culture. The fiber robot can generate tunable contractions upon stimulation. The surface of the fiber robot is formed by a braiding structure, which provides promising surface contact and adequate space for cell culture. An in-house dynamic stimulation using the fiber robot was set up to maintain NIH3T3 cells in a controlled environment. The biocompatibility of the developed dynamic culture systems was analyzed using LIVE/DEAD™ and alamarBlue™ assays. The results showed that the dynamic culture system was able to support cell proliferation with minimal cytotoxicity similar to static cultures. However, we observed a decrease in cell viability in the case of a high strain rate in dynamic cultures. Differences in cell arrangement and proliferation were observed between braided sleeves made of different materials (nylon and ultra-high molecular weight polyethylene). In summary, a simple and cost-effective 3D dynamic culture system has been proposed, which can be easily implemented to study complex biological phenomena in vitro.}, number={2}, journal={BIOMIMETICS}, author={Hoque, Muh Amdadul and Mahmood, Nasif and Ali, Kiran M. and Sefat, Eelya and Huang, Yihan and Petersen, Emily and Harrington, Shane and Fang, Xiaomeng and Gluck, Jessica M.}, year={2023}, month={Jun} }
 @article{huang_cadet_king_cole_2022, title={Comparison of the mechanical properties and anchoring performance of polyvinylidene fluoride and polypropylene barbed sutures for tendon repair}, volume={6}, ISSN={["1552-4981"]}, url={https://doi.org/10.1002/jbm.b.35074}, DOI={10.1002/jbm.b.35074}, abstractNote={Abstract Polyvinylidene fluoride (PVDF) has been considered as an alternative suture material to replace polypropylene (PP) due to its superior biocompatibility and mechanical properties, but it has never been examined for use in barbed sutures, particularly for tendon repair. This study fabricated size 2–0 PVDF and PP bidirectional barbed sutures and compared their mechanical properties and anchoring performance in patellar tendons. The mechanical properties were evaluated via tensile testing, and the anchoring performance of the barbed sutures was assessed by a tendon suture pullout test. Sixty porcine patellar tendons were harvested, transected to mimic a full‐thickness injury, and repaired using a cross‐locked cruciate suturing technique. The ultimate tensile force was 60% higher for the PVDF barbed sutures (22.4 ± 2.1 N) than for the PP barbed sutures (14.0 ± 1.7 N). The maximum pullout force was 35% higher for PVDF barbed sutures (70.8 ± 7.8 N) than for PP barbed sutures (52.4 ± 5.8 N). The force needed to form a 2‐mm gap, indicative of repair failure, was similar between the PVDF (29.2 ± 5.0 N) and PP (25.6 ± 3.1 N) barbed sutures, but both were greater than the 2‐mm‐gap forces for non‐barbed sutures of the same size. In this study, PVDF barbed sutures provided better mechanical properties and improved tissue anchoring performance compared to the barbed PP sutures for porcine patellar tendon repair, demonstrating that PVDF monofilament sutures can be barbed and used effectively for tendon repair.}, journal={JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS}, author={Huang, Yihan and Cadet, Edwin R. and King, Martin W. and Cole, Jacqueline H.}, year={2022}, month={Jun} }
 @article{ali_huang_amanah_mahmood_suh_gluck_2022, title={In Vitro Biocompatibility and Degradation Analysis of Mass-Produced Collagen Fibers}, volume={14}, ISSN={["2073-4360"]}, url={https://doi.org/10.3390/polym14102100}, DOI={10.3390/polym14102100}, abstractNote={Automation and mass-production are two of the many limitations in the tissue engineering industry. Textile fabrication methods such as electrospinning are used extensively in this field because of the resemblance of the extracellular matrix to the fiber structure. However, electrospinning has many limitations, including the ability to mass-produce, automate, and reproduce products. For this reason, this study evaluates the potential use of a traditional textile method such as spinning. Apart from mass production, these methods are also easy, efficient, and cost-effective. This study uses bovine-derived collagen fibers to create yarns using the traditional ring spinning method. The collagen yarns are proven to be biocompatible. Enzymatic biodegradability was also confirmed for its potential use in vivo. The results of this study prove the safety and efficacy of the material and the fabrication method. The material encourages higher cell proliferation and migration compared to tissue culture-treated plastic plates. The process is not only simple but is also streamlined and replicable, resulting in standardized products that can be reproduced.}, number={10}, journal={POLYMERS}, author={Ali, Kiran M. and Huang, Yihan and Amanah, Alaowei Y. and Mahmood, Nasif and Suh, Taylor C. and Gluck, Jessica M.}, year={2022}, month={May} }
 @article{mahmood_suh_ali_sefat_jahan_huang_gilger_gluck_2022, title={Induced Pluripotent Stem Cell-Derived Corneal Cells: Current Status and Application}, volume={8}, ISSN={["2629-3277"]}, url={https://doi.org/10.1007/s12015-022-10435-8}, DOI={10.1007/s12015-022-10435-8}, abstractNote={Deficiency and dysfunction of corneal cells leads to the blindness observed in corneal diseases such as limbal stem cell deficiency (LSCD) and bullous keratopathy. Regenerative cell therapies and engineered corneal tissue are promising treatments for these diseases [1]. However, these treatments are not yet clinically feasible due to inadequate cell sources. The discovery of induced pluripotent stem cells (iPSCs) by Shinya Yamanaka has provided a multitude of opportunities in research because iPSCs can be generated from somatic cells, thus providing an autologous and unlimited source for corneal cells. Compared to other stem cell sources such as mesenchymal and embryonic, iPSCs have advantages in differentiation potential and ethical concerns, respectively. Efforts have been made to use iPSCs to model corneal disorders and diseases, drug testing [2], and regenerative medicine [1]. Autologous treatments based on iPSCs can be exorbitantly expensive and time-consuming, but development of stem cell banks with human leukocyte antigen (HLA)- homozygous cell lines can provide cost- and time-efficient allogeneic alternatives. In this review, we discuss the early development of the cornea because protocols differentiating iPSCs toward corneal lineages rely heavily upon recapitulating this development. Differentiation of iPSCs toward corneal cell phenotypes have been analyzed with an emphasis on feeder-free, xeno-free, and well-defined protocols, which have clinical relevance. The application, challenges, and potential of iPSCs in corneal research are also discussed with a focus on hurdles that prevent clinical translation.}, journal={STEM CELL REVIEWS AND REPORTS}, author={Mahmood, Nasif and Suh, Taylor Cook and Ali, Kiran M. and Sefat, Eelya and Jahan, Ummay Mowshome and Huang, Yihan and Gilger, Brian C. and Gluck, Jessica M.}, year={2022}, month={Aug} }