@article{lai_ducoste_de los reyes iii_2023, title={Growth of Dunaliella viridis in multiple cycles of reclaimed media after repeated high pH-induced flocculation and harvesting}, volume={891}, ISSN={["1879-1026"]}, url={https://publons.com/wos-op/publon/61632805/}, DOI={10.1016/j.scitotenv.2023.164087}, abstractNote={Minimizing the use of water for growing microalgae is crucial for lowering the energy and costs of animal feed, food, and biofuel production from microalgae. Dunaliella spp., a haloterant species that can accumulate high intracellular levels of lipids, carotenoids, or glycerol can be harvested effectively using low-cost and scalable high pH-induced flocculation. However, the growth of Dunaliella spp. in reclaimed media after flocculation and the impact of recycling on the flocculation efficiency have not been explored. In this study, repeated cycles of growth of Dunaliella viridis in repeatedly reclaimed media from high pH-induced flocculation were studied by evaluating cell concentrations, cellular components, dissolved organic matter (DOM), and bacterial community shifts in the reclaimed media. In reclaimed media, D. viridis grew to the same concentrations of cells and intracellular components as fresh media-107 cells/mL with cellular composition of 3 % lipids, 40 % proteins, and 15 % carbohydrates-even though DOM accumulated and the dominant bacterial populations changed. There was a decrease in the maximum specific growth rate and flocculation efficiency from 0.72 d-1 to 0.45 d-1 and from 60 % to 48 %, respectively. This study shows the potential of repeated (at least five times) flocculation and reuse of media as a possible way of reducing the costs of water and nutrients with some tradeoffs in growth rate and flocculation efficiency.}, journal={SCIENCE OF THE TOTAL ENVIRONMENT}, author={Lai, Yi-Chun and Ducoste, Joel J. and de los Reyes III, Francis L.}, year={2023}, month={Sep} }
 @article{kotlarz_holcomb_pasha_reckling_kays_lai_daly_palani_bailey_guidry_et al._2023, title={Timing and Trends for Municipal Wastewater, Lab-Confirmed Case , and Syndromic Case Surveillance of COVID-19 in Raleigh, North Carolina}, volume={113}, ISSN={["1541-0048"]}, url={https://publons.com/wos-op/publon/54927766/}, DOI={10.2105/AJPH.2022.307108}, abstractNote={Objectives. To compare 4 COVID-19 surveillance metrics in a major metropolitan area. Methods. We analyzed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater influent and primary solids in Raleigh, North Carolina, from April 10 through December 13, 2020. We compared wastewater results with lab-confirmed COVID-19 cases and syndromic COVID-like illness (CLI) cases to answer 3 questions: (1) Did they correlate? (2) What was the temporal alignment of the different surveillance systems? (3) Did periods of significant change (i.e., trends) align? Results. In the Raleigh sewershed, wastewater influent, wastewater primary solids, lab-confirmed cases, and CLI were strongly or moderately correlated. Trends in lab-confirmed cases and wastewater influent were observed earlier, followed by CLI and, lastly, wastewater primary solids. All 4 metrics showed sustained increases in COVID-19 in June, July, and November 2020 and sustained decreases in August and September 2020. Conclusions. In a major metropolitan area in 2020, the timing of and trends in municipal wastewater, lab-confirmed case, and syndromic case surveillance of COVID-19 were in general agreement. Public Health Implications. Our results provide evidence for investment in SARS-CoV-2 wastewater and CLI surveillance to complement information provided through lab-confirmed cases. (Am J Public Health. Published online ahead of print November 10, 2022:e1-e11. https://doi.org/10.2105/AJPH.2022.307108).}, number={1}, journal={AMERICAN JOURNAL OF PUBLIC HEALTH}, publisher={American Public Health Association}, author={Kotlarz, Nadine and Holcomb, David A. and Pasha, Tanvir and Reckling, Stacie and Kays, Judith and Lai, Yi-Chun and Daly, Sean and Palani, Sivaranjani and Bailey, Erika and Guidry, Virginia T. and et al.}, year={2023}, month={Jan}, pages={79–88} }
 @article{karam_lai_reyes_ducoste_2021, title={Chlorophyll a and non-pigmented biomass are sufficient predictors for estimating light attenuation during cultivation of Dunaliella viridis}, volume={55}, ISSN={["2211-9264"]}, url={https://publons.com/wos-op/publon/46639302/}, DOI={10.1016/j.algal.2021.102283}, abstractNote={Characterizing light in microalgal cultivation vessels is needed for modeling and optimizing microalgal growth for large-scale cultivation. Dynamic changes in light intensity over space due to geometry, refraction/reflection, and the interactive impacts of algal growth and their biocomponents with light make this characterization challenging. Understanding which biocomponents within microalgal cultures are key variables in accurately estimating light attenuation is fundamentally important, yet, inconsistent and wide-ranging applications of the Beer-Lambert law are often used to estimate light attenuation. This research rigorously evaluated which biocomponents (total biomass, cell count, and chl a, chl b, and total photosynthesizing pigments), or biocomponent combinations, serve as best predictors for light attenuation when modeling with the Beer-Lambert law. Calibration and validation experiments were performed using salt-water species Dunaliella viridis microalgal cultures grown in 3-L flat-plate PBRs with continuous light monitoring. Results at the various light and nitrogen levels tested showed Beer-Lambert's law predicted photosynthetic light attenuation well when both biomass and chlorophyll a were considered as distinct attenuating components, providing light estimates with less than 6% error on average over validation experiments. If the model included only one component as a predictor for attenuation, pigments were best, with a 20% error in estimating light, as compared to ~70%, 60%, 40% for models that used solely biomass, cells, or chlorophyll a as an attenuating component., respectively. These results suggest that when using the Beer-Lambert's law to estimate photosynthetic light attenuation in microalgal cultures, both a chlorophyll a and biomass component should be consistently included.}, journal={ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS}, author={Karam, Amanda L. and Lai, Yi-Chun and Reyes, Francis L. I. I. I. I. I. I. and Ducoste, Joel J.}, year={2021}, month={May} }
 @article{karam_mcmillan_lai_reyes_sederoff_grunden_ranjithan_levis_ducoste_2017, title={Construction and setup of a bench-scale algal photosynthetic bioreactor with temperature, light, and ph monitoring for kinetic growth tests}, number={124}, journal={Jove-Journal of Visualized Experiments}, author={Karam, A. L. and McMillan, C. C. and Lai, Y. C. and Reyes, F. L. and Sederoff, H. W. and Grunden, A. M. and Ranjithan, R. S. and Levis, J. W. and Ducoste, J. J.}, year={2017} }